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The map label for this gene is nadE-2 [H]

Identifier: 197117472

GI number: 197117472

Start: 1259909

End: 1260895

Strand: Direct

Name: nadE-2 [H]

Synonym: Gbem_1083

Alternate gene names: 197117472

Gene position: 1259909-1260895 (Clockwise)

Preceding gene: 197117471

Following gene: 197117473

Centisome position: 27.3

GC content: 62.41

Gene sequence:

>987_bases
ATGAATGGTGGGATATTTTCCAAAGAAGTGCTCCGCCTGGACCCGGAACGGGAAGTGGAGCGTATTTGCGCCAGGGTGAA
AGAGCTGATGCTGACCCAGGTAAAGCGGCGCGGGTTGGTGGTGGCGCTTTCGGGAGGGATCGACAGCAGCGTTACTGCGG
CCCTGGCAGTGCGGGCGATCGGTAAGGAGCGGGTGATCGGCCTCGAGATGCCCGAGCGGCACTCGTCCGGCGAGAGCCAG
AAGCTAAGCGGCAAGCTGGCCGCCGCCCTCGGCATCGAGACGGTCCTGGAGGAGATCTCTGCAGCCCTGGAAGCGGTGGG
GTGCTATCGCAAGTACGATGAGGCGGTCAGGATGGTCGTCCCCGGCTACGGGGAGGGGTGGAAGTCCAAGATCGTGATCT
CCAACAACATGGAGCATCCCGGCTTCACCTCCTTTTACCTGGTGGCCCAGGACGGGGCCCAGGGCACCACCCGGGTCCGC
CTCCCCTTCAAGCCCTACCTGCAGATCGTTGCCGCCACCAACTTCAAACAGCGCATCCGCAAGATGCTGGAGTACTACCA
CGCCGATCGGCTCAACTTCGCCGTGGCAGGCACCCCCAACCGACTGGAGTACGACCAGGGGTTTTTTGTGAAACTCGGGG
ACGGAGCGGCCGACATCAAGCCGATCGCGCACCTGTACAAGTCGCAGGTGTACCAGTTGGCCGAATACCTGGGGGTGCCT
GAGGAGATCCGCCGCAGAACGCCCACCACGGACACCTATTCCCTGGCGCAAGGGCAGGACGAGTTCTACTTCTCGCTTCC
CTACCCGGAGATGGATCTCTGCCTTTTCGCCAAAAACAATGGAGTCGCGCCAGAAAGCGTGGCGGCAGCCCTCGGGCTCA
CCCCAGAGCAGGTCCGGCTGGTATTCCAGGACATCGAAGTAAAAAGGAGCAGCACCCGGTACCTGCACCTGCCTCCCTTG
CTGGTGGAGGCGGTCGACTGGCAGTGA

Upstream 100 bases:

>100_bases
CAAGATTGACCGCGCCGCCTGTTTAGAGGCGCCACCCCTGGCCGGCCCATGTTGTAGCGGCCGCCAGGGAGACTGAAGAA
AGGGTGAAAAGGGTGAACAT

Downstream 100 bases:

>100_bases
GGCCGGCATATCGAGACCAAAAAGGAGACGCACATGAGCCTGCAAGAAGATATCAGAAGCTTCATCGTCGACAATTTCCT
CTTCGGGGACGCCGGAACGC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MNGGIFSKEVLRLDPEREVERICARVKELMLTQVKRRGLVVALSGGIDSSVTAALAVRAIGKERVIGLEMPERHSSGESQ
KLSGKLAAALGIETVLEEISAALEAVGCYRKYDEAVRMVVPGYGEGWKSKIVISNNMEHPGFTSFYLVAQDGAQGTTRVR
LPFKPYLQIVAATNFKQRIRKMLEYYHADRLNFAVAGTPNRLEYDQGFFVKLGDGAADIKPIAHLYKSQVYQLAEYLGVP
EEIRRRTPTTDTYSLAQGQDEFYFSLPYPEMDLCLFAKNNGVAPESVAAALGLTPEQVRLVFQDIEVKRSSTRYLHLPPL
LVEAVDWQ

Sequences:

>Translated_328_residues
MNGGIFSKEVLRLDPEREVERICARVKELMLTQVKRRGLVVALSGGIDSSVTAALAVRAIGKERVIGLEMPERHSSGESQ
KLSGKLAAALGIETVLEEISAALEAVGCYRKYDEAVRMVVPGYGEGWKSKIVISNNMEHPGFTSFYLVAQDGAQGTTRVR
LPFKPYLQIVAATNFKQRIRKMLEYYHADRLNFAVAGTPNRLEYDQGFFVKLGDGAADIKPIAHLYKSQVYQLAEYLGVP
EEIRRRTPTTDTYSLAQGQDEFYFSLPYPEMDLCLFAKNNGVAPESVAAALGLTPEQVRLVFQDIEVKRSSTRYLHLPPL
LVEAVDWQ
>Mature_328_residues
MNGGIFSKEVLRLDPEREVERICARVKELMLTQVKRRGLVVALSGGIDSSVTAALAVRAIGKERVIGLEMPERHSSGESQ
KLSGKLAAALGIETVLEEISAALEAVGCYRKYDEAVRMVVPGYGEGWKSKIVISNNMEHPGFTSFYLVAQDGAQGTTRVR
LPFKPYLQIVAATNFKQRIRKMLEYYHADRLNFAVAGTPNRLEYDQGFFVKLGDGAADIKPIAHLYKSQVYQLAEYLGVP
EEIRRRTPTTDTYSLAQGQDEFYFSLPYPEMDLCLFAKNNGVAPESVAAALGLTPEQVRLVFQDIEVKRSSTRYLHLPPL
LVEAVDWQ

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 36564; Mature: 36564

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGGIFSKEVLRLDPEREVERICARVKELMLTQVKRRGLVVALSGGIDSSVTAALAVRAI
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHH
GKERVIGLEMPERHSSGESQKLSGKLAAALGIETVLEEISAALEAVGCYRKYDEAVRMVV
CCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
PGYGEGWKSKIVISNNMEHPGFTSFYLVAQDGAQGTTRVRLPFKPYLQIVAATNFKQRIR
CCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCEEEECCHHHHHHHHHHCCHHHHHH
KMLEYYHADRLNFAVAGTPNRLEYDQGFFVKLGDGAADIKPIAHLYKSQVYQLAEYLGVP
HHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
EEIRRRTPTTDTYSLAQGQDEFYFSLPYPEMDLCLFAKNNGVAPESVAAALGLTPEQVRL
HHHHHCCCCCCCCHHCCCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHH
VFQDIEVKRSSTRYLHLPPLLVEAVDWQ
HHHHHHHHCCCCCEEECCHHHHHHCCCC
>Mature Secondary Structure
MNGGIFSKEVLRLDPEREVERICARVKELMLTQVKRRGLVVALSGGIDSSVTAALAVRAI
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHH
GKERVIGLEMPERHSSGESQKLSGKLAAALGIETVLEEISAALEAVGCYRKYDEAVRMVV
CCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
PGYGEGWKSKIVISNNMEHPGFTSFYLVAQDGAQGTTRVRLPFKPYLQIVAATNFKQRIR
CCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCEEEECCHHHHHHHHHHCCHHHHHH
KMLEYYHADRLNFAVAGTPNRLEYDQGFFVKLGDGAADIKPIAHLYKSQVYQLAEYLGVP
HHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
EEIRRRTPTTDTYSLAQGQDEFYFSLPYPEMDLCLFAKNNGVAPESVAAALGLTPEQVRL
HHHHHCCCCCCCCHHCCCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHH
VFQDIEVKRSSTRYLHLPPLLVEAVDWQ
HHHHHHHHCCCCCEEECCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]