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The map label for this gene is cheB-4 [H]

Identifier: 197117427

GI number: 197117427

Start: 1208298

End: 1208867

Strand: Reverse

Name: cheB-4 [H]

Synonym: Gbem_1037

Alternate gene names: 197117427

Gene position: 1208867-1208298 (Counterclockwise)

Preceding gene: 197117428

Following gene: 197117425

Centisome position: 26.19

GC content: 65.61

Gene sequence:

>570_bases
ATGAGCGCTAGAAACCTGGTGGTCATCGGAGTTTCCACCGGTGGGCCGCTGACCCTCAAGGCCTTGTTCCGGGAGCTGCC
GGCGCTCGATGCGGCCTTCGTCGTGGTGCTCCACATCACGCCGCAGATGGACTACCGCATAGCCCAGGGGCTAGACGCGG
TCGCCTCCATGCCGGTAACGCTCGCCGAGGACCAGGAGTTCCTGAGAAGCGGCAGGATCTACCTGGCCCCCGGCGGGTTT
CACCTGAGGCTGAACGGCAACCAGCGGGTCGTGCTCTGCGAGGGGCCGCGCGTCAACTACGTCCAGCCGGCCGCCGACGT
CGCCATGCTCTCGCTCACCCGGCAGCTCAAAGGGAAGCTGGTCGGCATCGTCCTGACCGGCATGGGAAAGGACGGCGCCG
AGGGGATCCGGCACATCAAGCGGCTAGGAGGGGTCACCATGGCGCAGGACCAGCAATCCTCCACCATCTACGGCATGCCG
AAAGCGGCCGCCGACACAGGCGCCGTCGACTTCGTGCTCCCCCCGCAAAAGATCGCCGCCAAATTAAAGGAGATCCTGGA
CCCGCTTTAG

Upstream 100 bases:

>100_bases
CAGGAATACGTCTCGGACTACATGACCAAGCCCTTCGGCCCGGACCAGCTGATCGAATCGCTTGAGTACTACCTTACCCT
TTTGAACACCCCGGTGGACT

Downstream 100 bases:

>100_bases
CCGCTCAGCCGGTTTCCGCCTGGCTGCGCCACTCCTCCGCCGCATGCTGCAGCAGGAAGTCGACGAAGGCGCGGTTGGCA
AGGGAGAGATAGGTATCCTT

Product: chemotaxis protein-glutamate methylesterase CheB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MSARNLVVIGVSTGGPLTLKALFRELPALDAAFVVVLHITPQMDYRIAQGLDAVASMPVTLAEDQEFLRSGRIYLAPGGF
HLRLNGNQRVVLCEGPRVNYVQPAADVAMLSLTRQLKGKLVGIVLTGMGKDGAEGIRHIKRLGGVTMAQDQQSSTIYGMP
KAAADTGAVDFVLPPQKIAAKLKEILDPL

Sequences:

>Translated_189_residues
MSARNLVVIGVSTGGPLTLKALFRELPALDAAFVVVLHITPQMDYRIAQGLDAVASMPVTLAEDQEFLRSGRIYLAPGGF
HLRLNGNQRVVLCEGPRVNYVQPAADVAMLSLTRQLKGKLVGIVLTGMGKDGAEGIRHIKRLGGVTMAQDQQSSTIYGMP
KAAADTGAVDFVLPPQKIAAKLKEILDPL
>Mature_188_residues
SARNLVVIGVSTGGPLTLKALFRELPALDAAFVVVLHITPQMDYRIAQGLDAVASMPVTLAEDQEFLRSGRIYLAPGGFH
LRLNGNQRVVLCEGPRVNYVQPAADVAMLSLTRQLKGKLVGIVLTGMGKDGAEGIRHIKRLGGVTMAQDQQSSTIYGMPK
AAADTGAVDFVLPPQKIAAKLKEILDPL

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR [H]

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=185, Percent_Identity=32.4324324324324, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789 [H]

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg [H]

EC number: =3.1.1.61 [H]

Molecular weight: Translated: 20171; Mature: 20040

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS00120 LIPASE_SER ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARNLVVIGVSTGGPLTLKALFRELPALDAAFVVVLHITPQMDYRIAQGLDAVASMPVT
CCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHCHHHHHHCCEE
LAEDQEFLRSGRIYLAPGGFHLRLNGNQRVVLCEGPRVNYVQPAADVAMLSLTRQLKGKL
ECCCHHHHHCCCEEEECCCEEEEECCCCEEEEECCCCCCEECCHHHHHHHHHHHHHCCEE
VGIVLTGMGKDGAEGIRHIKRLGGVTMAQDQQSSTIYGMPKAAADTGAVDFVLPPQKIAA
EEEEEEECCCCHHHHHHHHHHHCCCEEECCCCCCEEECCCCCCCCCCCEEEEECCHHHHH
KLKEILDPL
HHHHHHCCC
>Mature Secondary Structure 
SARNLVVIGVSTGGPLTLKALFRELPALDAAFVVVLHITPQMDYRIAQGLDAVASMPVT
CCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHCHHHHHHCCEE
LAEDQEFLRSGRIYLAPGGFHLRLNGNQRVVLCEGPRVNYVQPAADVAMLSLTRQLKGKL
ECCCHHHHHCCCEEEECCCEEEEECCCCEEEEECCCCCCEECCHHHHHHHHHHHHHCCEE
VGIVLTGMGKDGAEGIRHIKRLGGVTMAQDQQSSTIYGMPKAAADTGAVDFVLPPQKIAA
EEEEEEECCCCHHHHHHHHHHHCCCEEECCCCCCEEECCCCCCCCCCCEEEEECCHHHHH
KLKEILDPL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA