Click here to switch to the map view.

The map label for this gene is eno [H]

Identifier: 197117386

GI number: 197117386

Start: 1152478

End: 1153767

Strand: Reverse

Name: eno [H]

Synonym: Gbem_0996

Alternate gene names: 197117386

Gene position: 1153767-1152478 (Counterclockwise)

Preceding gene: 308535210

Following gene: 197117385

Centisome position: 25.0

GC content: 60.23

Gene sequence:

>1290_bases
ATGAGCCAGATAACCGACGTTTATGCCAGAGAGATCCTTGATTCCAGGGGGAATCCTACGCTTGAAGTAGAGGTATTCCT
GGATTCCGGCGTTATGGGAAGGGCTGCGGTTCCGTCCGGCGCATCGACCGGCGAGCGCGAAGCACTGGAGCTGCGTGACG
GCGACAAGGGGCGCTACCTCGGCAAAGGCGTGGAACAGGCTGTCTCCAACGTTAACGACATCATCGCCGACGAGATCACC
GGCATGGATGCGACCGACCAGGTTGGGATCGACAAGAAGATGCTGGAGCTCGACGGCACCGAATTCAAGAGCCGCCTGGG
CGCCAACGCCATCCTCGGCGTTTCCCTGGCCGTAGCCAAGGCCGCAGCGGAAGAAGTCGGAGTGCCGCTGTACCAGTACA
TCGGCGGATGCAACGCAAAAGAGCTGCCGCTGCCCATGATGAACATCATCAACGGCGGGGCCCACGCCGACAACAACGTC
GACATCCAGGAATTCATGATCATGCCCGCCGGCGCCGCCAACTTCAAGGAAGCTCTCAGGATGGGCGCGGAGATTTTTCA
CGCGCTGAAGAGCGTCCTCAAGGGCAAAGGGTACAACACGGCGGTCGGCGACGAAGGGGGCTTCGCGCCGAACCTCAAGT
CCAATGAGGAAGCGCTGGAAGTCATCATGGAAGCCATCGTCAAGGCCGGCTACAAGCCGGGCGAAGAAGTACTTTTGGCG
CTCGACGTCGCGTCTTCCGAACTCTTCGAAAACGGCGTCTACACCCTCGAGAACGAGGCGGAGTCCAAGAAGACCGCGGA
CCAGCTGGTCGACTTCTACGAGAACCTCGTCAACAAGTACCCCATCGTCTCCATCGAAGACGGCATGGCCGAAAACGACT
GGGACGGCTGGAAAAAGCTCACCGACCGCCTCGGCAAACGCATCCAGATCGTGGGCGACGACCTGTTCGTCACCAACCCC
TCCATCCTCAAGGAAGGGATCAAGAAAGGGATCGCCAACTCCATCCTGATCAAGCTGAACCAGATCGGCACCCTGACCGA
GACCCTCGACGCCATCGAGATGGCCAAGCGCGCCGGCTACACCTGCGTCATCTCGCACCGCTCCGGCGAGACCGAGGACA
CCACCCTTGCCGACCTGGCAGTGGCGGTTAACGCGGGCCAGATCAAGACCGGTTCGCTCTGCCGCACCGACCGCGTCTGC
AAGTACAACCAGCTCCTCAGGATCGAGGACGAGCTGGACGATGTTGCGCAGTTCCGCGGGCACGAGGTCTTCTACAACAT
CAAGAAGTAA

Upstream 100 bases:

>100_bases
AAGTCCCTTCCATTTAGGTGCAGAAATGTTATATAACGGAACTCAATTAAGAGGGGGCGTCGTAGTCTCTTCGTAATTTA
ATTCAACCTAGGGGGAACAG

Downstream 100 bases:

>100_bases
TCGGGTTCCATTCATAGCTGACAAAAGGGGCGCCTCCAGACGGGGGGCGCCCTTTTGTTTTCCGCCCGCGGCGTTTTTGC
AGCAGCGGCTCGCCCGGGCT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSQITDVYAREILDSRGNPTLEVEVFLDSGVMGRAAVPSGASTGEREALELRDGDKGRYLGKGVEQAVSNVNDIIADEIT
GMDATDQVGIDKKMLELDGTEFKSRLGANAILGVSLAVAKAAAEEVGVPLYQYIGGCNAKELPLPMMNIINGGAHADNNV
DIQEFMIMPAGAANFKEALRMGAEIFHALKSVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMEAIVKAGYKPGEEVLLA
LDVASSELFENGVYTLENEAESKKTADQLVDFYENLVNKYPIVSIEDGMAENDWDGWKKLTDRLGKRIQIVGDDLFVTNP
SILKEGIKKGIANSILIKLNQIGTLTETLDAIEMAKRAGYTCVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVC
KYNQLLRIEDELDDVAQFRGHEVFYNIKK

Sequences:

>Translated_429_residues
MSQITDVYAREILDSRGNPTLEVEVFLDSGVMGRAAVPSGASTGEREALELRDGDKGRYLGKGVEQAVSNVNDIIADEIT
GMDATDQVGIDKKMLELDGTEFKSRLGANAILGVSLAVAKAAAEEVGVPLYQYIGGCNAKELPLPMMNIINGGAHADNNV
DIQEFMIMPAGAANFKEALRMGAEIFHALKSVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMEAIVKAGYKPGEEVLLA
LDVASSELFENGVYTLENEAESKKTADQLVDFYENLVNKYPIVSIEDGMAENDWDGWKKLTDRLGKRIQIVGDDLFVTNP
SILKEGIKKGIANSILIKLNQIGTLTETLDAIEMAKRAGYTCVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVC
KYNQLLRIEDELDDVAQFRGHEVFYNIKK
>Mature_428_residues
SQITDVYAREILDSRGNPTLEVEVFLDSGVMGRAAVPSGASTGEREALELRDGDKGRYLGKGVEQAVSNVNDIIADEITG
MDATDQVGIDKKMLELDGTEFKSRLGANAILGVSLAVAKAAAEEVGVPLYQYIGGCNAKELPLPMMNIINGGAHADNNVD
IQEFMIMPAGAANFKEALRMGAEIFHALKSVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMEAIVKAGYKPGEEVLLAL
DVASSELFENGVYTLENEAESKKTADQLVDFYENLVNKYPIVSIEDGMAENDWDGWKKLTDRLGKRIQIVGDDLFVTNPS
ILKEGIKKGIANSILIKLNQIGTLTETLDAIEMAKRAGYTCVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVCK
YNQLLRIEDELDDVAQFRGHEVFYNIKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=53.0232558139535, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=53.0232558139535, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI301897477, Length=434, Percent_Identity=52.073732718894, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI301897469, Length=434, Percent_Identity=52.073732718894, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI301897479, Length=432, Percent_Identity=47.9166666666667, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI169201331, Length=350, Percent_Identity=26.8571428571429, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI169201757, Length=350, Percent_Identity=26.8571428571429, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI239744207, Length=350, Percent_Identity=26.8571428571429, Blast_Score=112, Evalue=9e-25,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=63.849765258216, Blast_Score=533, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=52.4361948955916, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=52.4361948955916, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=45.5497382198953, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=437, Percent_Identity=50.5720823798627, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=48.2678983833718, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=48.2678983833718, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=48.2678983833718, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=49.8855835240275, Blast_Score=376, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=429, Percent_Identity=51.5151515151515, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580916, Length=429, Percent_Identity=51.5151515151515, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580920, Length=429, Percent_Identity=51.5151515151515, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580914, Length=429, Percent_Identity=51.5151515151515, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281360527, Length=429, Percent_Identity=51.5151515151515, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=429, Percent_Identity=51.5151515151515, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46492; Mature: 46361

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQITDVYAREILDSRGNPTLEVEVFLDSGVMGRAAVPSGASTGEREALELRDGDKGRYL
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHH
GKGVEQAVSNVNDIIADEITGMDATDQVGIDKKMLELDGTEFKSRLGANAILGVSLAVAK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHH
AAAEEVGVPLYQYIGGCNAKELPLPMMNIINGGAHADNNVDIQEFMIMPAGAANFKEALR
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHEEECCCCHHHHHHHH
MGAEIFHALKSVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMEAIVKAGYKPGEEVLLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEEE
LDVASSELFENGVYTLENEAESKKTADQLVDFYENLVNKYPIVSIEDGMAENDWDGWKKL
EEHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHH
TDRLGKRIQIVGDDLFVTNPSILKEGIKKGIANSILIKLNQIGTLTETLDAIEMAKRAGY
HHHHCCEEEEEECCEEECCHHHHHHHHHHHCHHHHEEEEHHCCCHHHHHHHHHHHHHCCC
TCVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVCKYNQLLRIEDELDDVAQFRG
EEEEECCCCCCCCHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHEEEHHHHHHHHHHCC
HEVFYNIKK
CEEEEEECC
>Mature Secondary Structure 
SQITDVYAREILDSRGNPTLEVEVFLDSGVMGRAAVPSGASTGEREALELRDGDKGRYL
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHH
GKGVEQAVSNVNDIIADEITGMDATDQVGIDKKMLELDGTEFKSRLGANAILGVSLAVAK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHH
AAAEEVGVPLYQYIGGCNAKELPLPMMNIINGGAHADNNVDIQEFMIMPAGAANFKEALR
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHEEECCCCHHHHHHHH
MGAEIFHALKSVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMEAIVKAGYKPGEEVLLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEEE
LDVASSELFENGVYTLENEAESKKTADQLVDFYENLVNKYPIVSIEDGMAENDWDGWKKL
EEHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHH
TDRLGKRIQIVGDDLFVTNPSILKEGIKKGIANSILIKLNQIGTLTETLDAIEMAKRAGY
HHHHCCEEEEEECCEEECCHHHHHHHHHHHCHHHHEEEEHHCCCHHHHHHHHHHHHHCCC
TCVISHRSGETEDTTLADLAVAVNAGQIKTGSLCRTDRVCKYNQLLRIEDELDDVAQFRG
EEEEECCCCCCCCHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHEEEHHHHHHHHHHCC
HEVFYNIKK
CEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA