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The map label for this gene is tuf-1 [H]

Identifier: 197117308

GI number: 197117308

Start: 1080005

End: 1081195

Strand: Direct

Name: tuf-1 [H]

Synonym: Gbem_0918

Alternate gene names: 197117308

Gene position: 1080005-1081195 (Clockwise)

Preceding gene: 197117306

Following gene: 197117309

Centisome position: 23.4

GC content: 61.88

Gene sequence:

>1191_bases
ATGGCTAAAGCTAAATTCGAAAGAACTAAACCGCATGTGAACATAGGGACCATCGGTCACGTCGACCACGGCAAGACCAC
CCTGACCGCAGCAATCACCAAGGTACTCGCAGGCAAAGGCCAGGCCGAGTTCAAGGCGTTCGACCAGATCGACAACGCAC
CGGAAGAGCGCGAGCGTGGTATCACCATCGCTACCGCCCATGTCGAGTACGAGACCGACAAGCGTCACTACGCTCACGTC
GACTGCCCGGGTCACGCCGACTACGTTAAGAACATGATCACCGGTGCGGCGCAGATGGACGGCGCCATCCTGGTTGTTTC
CGCAGCTGACGGCCCGATGCCGCAGACCCGCGAGCACATCCTGCTTGCGCGTCAGGTCGGCGTCCCCTACATCGTCGTGT
TCCTGAACAAGGCCGACATGGTGGACGACGAGGAGCTCCTCGAGCTGGTCGAGCTGGAAGTTCGCGAACTCCTCTCCTCC
TACGACTTCCCGGGCGACGATATCCCCATCATCAAGGGTTCCGCTCTCAAAGGGCTCGAAGGCGACGCCGGCGAGCTGGG
CGAGCAGGCAATCATGAAACTGATGGAAGCTGTCGACACCTACATCCCGGAGCCGGTTCGCGCCATCGACAAGCCGTTCC
TGATGCCGGTCGAGGACGTGTTCTCCATCTCCGGTCGCGGCACCGTCGCAACCGGTCGTGTCGAGCGCGGCATCGTGAAA
GTCGGCGAGGAGGTCGAGATCGTCGGCATGAAGGCCACCGCCAAGACCACCGTCACCGGCGTCGAGATGTTCCGTAAGCT
CCTCGACGAAGGTCGCGCAGGCGACAACATCGGCGCGCTGCTGCGCGGCGTGAAGCGTGAAGAGATCGAGCGCGGCCAGG
TTCTGGCCAAGCCGGGCTCCATCACCCCGCACACCAAGTTCAAGGCAGAAGCCTACATCCTCTCCAAAGAGGAAGGCGGC
CGTCACACCCCGTTCTTCAACGGCTATCGCCCGCAGTTCTACTTCAGGACCACCGACGTGACCGGGATCGTTGACCTCGA
GGCAGGCGTCGAGATGGTTATGCCTGGCGACAACGTCTCGGTGACCGTCAACCTGATCACCCCGATCGCGATGGACGAAG
GTCTGCGTTTCGCAATCCGCGAAGGCGGCCGCACCGTCGGCGCCGGTGTCGTAGCCTCCATCATCGAGTAA

Upstream 100 bases:

>100_bases
GGTCACCGGTTCGAATCCGGTTGTGGGCTCCACTCTCTTTTTTAAAAAACAAACAAAAGCTATCCATAAAAAGCTGTCGA
TACATCAGGGAGGATTCCTC

Downstream 100 bases:

>100_bases
GGTACATCGAGCGCGCGTTTCGTTGCCGGTTTCCCCGGTGCGAAACGCGATGCTCTTTTTAAATTAGTTTGAAAAGGACA
CAAAGACATGCCTAGAGACA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERGITIATAHVEYETDKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVELEVRELLSS
YDFPGDDIPIIKGSALKGLEGDAGELGEQAIMKLMEAVDTYIPEPVRAIDKPFLMPVEDVFSISGRGTVATGRVERGIVK
VGEEVEIVGMKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREEIERGQVLAKPGSITPHTKFKAEAYILSKEEGG
RHTPFFNGYRPQFYFRTTDVTGIVDLEAGVEMVMPGDNVSVTVNLITPIAMDEGLRFAIREGGRTVGAGVVASIIE

Sequences:

>Translated_396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERGITIATAHVEYETDKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVELEVRELLSS
YDFPGDDIPIIKGSALKGLEGDAGELGEQAIMKLMEAVDTYIPEPVRAIDKPFLMPVEDVFSISGRGTVATGRVERGIVK
VGEEVEIVGMKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREEIERGQVLAKPGSITPHTKFKAEAYILSKEEGG
RHTPFFNGYRPQFYFRTTDVTGIVDLEAGVEMVMPGDNVSVTVNLITPIAMDEGLRFAIREGGRTVGAGVVASIIE
>Mature_395_residues
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERGITIATAHVEYETDKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVELEVRELLSSY
DFPGDDIPIIKGSALKGLEGDAGELGEQAIMKLMEAVDTYIPEPVRAIDKPFLMPVEDVFSISGRGTVATGRVERGIVKV
GEEVEIVGMKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREEIERGQVLAKPGSITPHTKFKAEAYILSKEEGGR
HTPFFNGYRPQFYFRTTDVTGIVDLEAGVEMVMPGDNVSVTVNLITPIAMDEGLRFAIREGGRTVGAGVVASIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=58.6294416243655, Blast_Score=491, Evalue=1e-139,
Organism=Homo sapiens, GI4503475, Length=454, Percent_Identity=30.6167400881057, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=30.1339285714286, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=30.7865168539326, Blast_Score=174, Evalue=9e-44,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=30.7865168539326, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI194097354, Length=438, Percent_Identity=28.5388127853881, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI194018522, Length=438, Percent_Identity=28.5388127853881, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI194018520, Length=438, Percent_Identity=28.5388127853881, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI46094014, Length=439, Percent_Identity=28.4738041002278, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=28.9855072463768, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI4503507, Length=274, Percent_Identity=29.9270072992701, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI157426893, Length=370, Percent_Identity=25.6756756756757, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI45593140, Length=421, Percent_Identity=22.5653206650831, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=80, Blast_Score=623, Evalue=1e-180,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=80, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI2367247, Length=378, Percent_Identity=30.4232804232804, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI48994988, Length=252, Percent_Identity=33.7301587301587, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789108, Length=326, Percent_Identity=28.8343558282209, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788922, Length=145, Percent_Identity=34.4827586206897, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.6290726817043, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.9948320413437, Blast_Score=350, Evalue=6e-97,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=31.6964285714286, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=31.6964285714286, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=30.2325581395349, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=26.9911504424779, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=445, Percent_Identity=27.6404494382022, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=445, Percent_Identity=27.6404494382022, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=29.0969899665552, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=40.6896551724138, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=281, Percent_Identity=26.6903914590747, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=29.3617021276596, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=68.0904522613065, Blast_Score=547, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=30.4545454545455, Blast_Score=172, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=30.4545454545455, Blast_Score=172, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6322937, Length=452, Percent_Identity=24.7787610619469, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=340, Percent_Identity=31.1764705882353, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=282, Percent_Identity=28.3687943262411, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=30.8641975308642, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=200, Percent_Identity=32, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.5419847328244, Blast_Score=496, Evalue=1e-140,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.5419847328244, Blast_Score=496, Evalue=1e-140,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=50.5208333333333, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=30.0668151447661, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=30.0668151447661, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=30.0668151447661, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=30.0668151447661, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=30.3571428571429, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=30.3571428571429, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=32.1311475409836, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI45550900, Length=338, Percent_Identity=29.585798816568, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI17137380, Length=337, Percent_Identity=29.080118694362, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24647046, Length=281, Percent_Identity=28.4697508896797, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24647048, Length=281, Percent_Identity=28.4697508896797, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28572034, Length=232, Percent_Identity=32.7586206896552, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42997; Mature: 42866

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCC
ITIATAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDEELLELVELEVRELLSSYDFPGDDIPIIKGSALKGLE
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
GDAGELGEQAIMKLMEAVDTYIPEPVRAIDKPFLMPVEDVFSISGRGTVATGRVERGIVK
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHCCCCCCEEEECHHHHHHHH
VGEEVEIVGMKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREEIERGQVLAKPGS
CCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCC
ITPHTKFKAEAYILSKEEGGRHTPFFNGYRPQFYFRTTDVTGIVDLEAGVEMVMPGDNVS
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCEEEECCCCCE
VTVNLITPIAMDEGLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEECCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCC
ITIATAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDEELLELVELEVRELLSSYDFPGDDIPIIKGSALKGLE
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
GDAGELGEQAIMKLMEAVDTYIPEPVRAIDKPFLMPVEDVFSISGRGTVATGRVERGIVK
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHCCCCCCEEEECHHHHHHHH
VGEEVEIVGMKATAKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREEIERGQVLAKPGS
CCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEECCCC
ITPHTKFKAEAYILSKEEGGRHTPFFNGYRPQFYFRTTDVTGIVDLEAGVEMVMPGDNVS
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCEEEECCCCCE
VTVNLITPIAMDEGLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEECCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA