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The map label for this gene is truA

Identifier: 197117301

GI number: 197117301

Start: 1073431

End: 1074183

Strand: Direct

Name: truA

Synonym: Gbem_0911

Alternate gene names: 197117301

Gene position: 1073431-1074183 (Clockwise)

Preceding gene: 197117300

Following gene: 197117302

Centisome position: 23.26

GC content: 64.01

Gene sequence:

>753_bases
ATGCGTAACATCAAGCTGATCATAGAATACGATGGCACCGCCTATTGCGGCTGGCAGGTGCAGCCGAACGGCCGCACGGT
CCAGGAGGTATTGCAGGAGGCCTTGGCGGCCATGCTGGGGGAGAAGACCCCCCTGCACGGCTCCGGCCGCACCGATGCGG
GCGTGCATGCCCGCGGCATGGTGGCCTGCTTTAAAACCGATAAGGCCATGCCGCTGCGCGCCTTCCGGGAGGGGCTCAAT
TGCCTCCTTCCAGGCGACATCGCGGTGCGGGAGGCGTGCGAGGTCCCGCTGGAATTCCATCCCCGCTTCGACGCGCACGC
GAAGCACTACCGCTACACCATTCTCTTGGACGACCTGCGCTCCCCGCTGTCGCGCCTCACGGTCTGGCGCCTTAAGGGAA
AGCTCGACATCCAGGCGATGAGAGCCGCCTGCGCCGCTTTCGTCGGCGAGCACGACTTCGCCGCCTTCCGTGCTTCCAAC
TGCGCCGCCAAGACCACGGTGCGCAGGATCTACAGCATGGACCTGGTGCAGGAGGGGTGCTTGCTGCATCTGGACGTGAA
GGGGAGCGGCTTTCTGAAGAACATGGTCCGCATCATCACCGGGACCCTGATCGAGGTGGGGCAGGGGAAGAAAAGCGTCG
AGGACGTGGCGCGACTGCTTCAAGGGGGCGACCGGCAGCAGAACTCCGGCATGACTGTGCCTCCGCAGGGGCTTTGCCTG
ATGCAGGTCTACTATCAGGAAAAGTGCGACTGA

Upstream 100 bases:

>100_bases
GCTGTCGGACAGGTCAGACAGGTCAGACAGGTCAGACAGGTCAGACAGGTCAGACAGGTCAGACAGGTCAGACAGGTCAG
ACAGGTCCGCGGAAAGAGTT

Downstream 100 bases:

>100_bases
TACTGCTTTTTATAAACCGTTAATCTTTCCTTTCGGTTTTTTGTCTTGACATGAAGTGGGGAATGGATTAGATTCTCAAA
CTTCCGTAAGTTAATGTGCT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MRNIKLIIEYDGTAYCGWQVQPNGRTVQEVLQEALAAMLGEKTPLHGSGRTDAGVHARGMVACFKTDKAMPLRAFREGLN
CLLPGDIAVREACEVPLEFHPRFDAHAKHYRYTILLDDLRSPLSRLTVWRLKGKLDIQAMRAACAAFVGEHDFAAFRASN
CAAKTTVRRIYSMDLVQEGCLLHLDVKGSGFLKNMVRIITGTLIEVGQGKKSVEDVARLLQGGDRQQNSGMTVPPQGLCL
MQVYYQEKCD

Sequences:

>Translated_250_residues
MRNIKLIIEYDGTAYCGWQVQPNGRTVQEVLQEALAAMLGEKTPLHGSGRTDAGVHARGMVACFKTDKAMPLRAFREGLN
CLLPGDIAVREACEVPLEFHPRFDAHAKHYRYTILLDDLRSPLSRLTVWRLKGKLDIQAMRAACAAFVGEHDFAAFRASN
CAAKTTVRRIYSMDLVQEGCLLHLDVKGSGFLKNMVRIITGTLIEVGQGKKSVEDVARLLQGGDRQQNSGMTVPPQGLCL
MQVYYQEKCD
>Mature_250_residues
MRNIKLIIEYDGTAYCGWQVQPNGRTVQEVLQEALAAMLGEKTPLHGSGRTDAGVHARGMVACFKTDKAMPLRAFREGLN
CLLPGDIAVREACEVPLEFHPRFDAHAKHYRYTILLDDLRSPLSRLTVWRLKGKLDIQAMRAACAAFVGEHDFAAFRASN
CAAKTTVRRIYSMDLVQEGCLLHLDVKGSGFLKNMVRIITGTLIEVGQGKKSVEDVARLLQGGDRQQNSGMTVPPQGLCL
MQVYYQEKCD

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=248, Percent_Identity=30.6451612903226, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI1788657, Length=247, Percent_Identity=39.2712550607287, Blast_Score=171, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_GEOBB (B5EFM8)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137728.1
- GeneID:   6783748
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0911
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27770; Mature: 27770

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIKLIIEYDGTAYCGWQVQPNGRTVQEVLQEALAAMLGEKTPLHGSGRTDAGVHARGM
CCCEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
VACFKTDKAMPLRAFREGLNCLLPGDIAVREACEVPLEFHPRFDAHAKHYRYTILLDDLR
EEEEECCCCCCHHHHHCCCCEECCCHHHHHHHHCCCCEECCCCCCCCCCEEEEEEHHHHH
SPLSRLTVWRLKGKLDIQAMRAACAAFVGEHDFAAFRASNCAAKTTVRRIYSMDLVQEGC
HHHHHHEEHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
LLHLDVKGSGFLKNMVRIITGTLIEVGQGKKSVEDVARLLQGGDRQQNSGMTVPPQGLCL
EEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
MQVYYQEKCD
HHHHHHHCCC
>Mature Secondary Structure
MRNIKLIIEYDGTAYCGWQVQPNGRTVQEVLQEALAAMLGEKTPLHGSGRTDAGVHARGM
CCCEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
VACFKTDKAMPLRAFREGLNCLLPGDIAVREACEVPLEFHPRFDAHAKHYRYTILLDDLR
EEEEECCCCCCHHHHHCCCCEECCCHHHHHHHHCCCCEECCCCCCCCCCEEEEEEHHHHH
SPLSRLTVWRLKGKLDIQAMRAACAAFVGEHDFAAFRASNCAAKTTVRRIYSMDLVQEGC
HHHHHHEEHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
LLHLDVKGSGFLKNMVRIITGTLIEVGQGKKSVEDVARLLQGGDRQQNSGMTVPPQGLCL
EEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
MQVYYQEKCD
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA