Click here to switch to the map view.

The map label for this gene is 197117268

Identifier: 197117268

GI number: 197117268

Start: 1031721

End: 1033142

Strand: Direct

Name: 197117268

Synonym: Gbem_0878

Alternate gene names: NA

Gene position: 1031721-1033142 (Clockwise)

Preceding gene: 308535201

Following gene: 197117269

Centisome position: 22.36

GC content: 62.87

Gene sequence:

>1422_bases
ATGAAGCCGCTATTTCTGAACCCGCCGACCTTCGAGGACTTCGACGGGGGGGCCGGTGCGCGCTACCAAGCCTCCCGCGA
GGTTACCTCCTTCTGGTTTCCCGGCTGGCTTACCTATCCCGCAGGGATGATCCCCGGAGCCCGGGTCGTGGATGCCCCGG
TGCAGCGGCTCGATCTCGATGCCTGCCTGAAGATCGCCAAAGAGTTCGACATGGTGGTGATGTACACCTCCACCCCGACG
CTCGCCATCGACGTAGAAACCGCCCGCCGCGTCAAGGCCCAGAACCCCGCCACCGTCACGGTGCTGACCGGCCCCCACGT
GAGCGTCCTCCCCGAGGAATCGCTTCGCTTCGCCGCCGGCGCCGTCGACATAGTCTGCCGCGGCGAGTTCGACTACTCCA
CCAAGGAGCTTTGCGAAGGGAAGCCGCGTGCGGAGGTGGAGGGCATCAGCTTTCTGCAGGACGGGAAGGTGGTGCACACC
AAGGACCGCCCCCCCATCGCCGACCTCGACAGCCTCCCCTTTGCAAGCCAGGTCTACCATCGCGACCTTCCCATCGATGA
GTACGTCATCCCGCATTTCCGGCACCCTTACGTCTCCATCTACGCCTCCCGCGGCTGCCCCTCGCGCTGCATCTACTGCC
TGTGGCCCCAGACCTTCTCCGGGCGGACCCTGAGGAAGAGGAGCCCGCAGAACGTGTATGAAGAGGTGCGCTGGATCAAG
GAGAACCTCCCCCAGGTGAAGGAGATCTCCTTCGACGACGACACCTTCACCGCCGACCGCGAGCACGCCAAGGCGATTGC
CCGGGCCATCAAGCCGCTCAACGTCTCCTGGGTGATCAACGCGCGGGCCAACTGCGACTACGAGACGCTCAAGGAGTTGC
GCGACGCCGGGATGCACCACGTGGTGGTCGGCTACGAGACGGGGAACGAGCAGATCCTGAAGAACATCAAGAAAGGGGTC
ACCAAGGCGCAGGCCATCGAGTTCACCCGCAACTGCCACAAGCTCGGCCTCACCATACATGGCGCTTTCGTGCTGGGGCT
CCCCGGGGAGACCAGGGAGACCATCAAGGAGACCATCGCCTACGCCATAGATCTCAACCTCACCTCGATCCAGGTCTCGC
TCGCCTCCCCCTATCCCGGCACCGAGTTCTACGATATGGCGCTCAAGGAGGGGTGGATCGCCTCGGACAGCTTCCTGGAT
GCCAGCGGGCACCAGAAATGCGTCATCAACTATCCCGACCTCTCCAATAAGGAGATCTTCGACGCGGTCGAACTCTTCTA
CAACAAGTTCTATTTCCGCCCCCGTTACATCGCGCGCAGCCTCTTCAGCATGCTGGTCGACTCCGCCCAGCGCAAGAAGC
TTCTCAAGGAGGGGGCGCAGTACCTAAGCTACATGAGAAAGCGCAAGCAGACCTGCGCCTAG

Upstream 100 bases:

>100_bases
TGCCGCAATGTCAAAGAGGGTTAACTTGACAAACTGGATGCGCCGACATATAGTCAGGCCTCTAATTTTTGTGTGATACT
GCTAATCTGGAGGGTGAATC

Downstream 100 bases:

>100_bases
GAAGTCGATGAAAGAGCTAGTGATCAACGCGGACGACTTCGGTCTTTCCAATGGCGCCAACGAGGGCGTCTATCGGGGGT
GGGAGGACGGGGTACTGACC

Product: radical SAM protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKPLFLNPPTFEDFDGGAGARYQASREVTSFWFPGWLTYPAGMIPGARVVDAPVQRLDLDACLKIAKEFDMVVMYTSTPT
LAIDVETARRVKAQNPATVTVLTGPHVSVLPEESLRFAAGAVDIVCRGEFDYSTKELCEGKPRAEVEGISFLQDGKVVHT
KDRPPIADLDSLPFASQVYHRDLPIDEYVIPHFRHPYVSIYASRGCPSRCIYCLWPQTFSGRTLRKRSPQNVYEEVRWIK
ENLPQVKEISFDDDTFTADREHAKAIARAIKPLNVSWVINARANCDYETLKELRDAGMHHVVVGYETGNEQILKNIKKGV
TKAQAIEFTRNCHKLGLTIHGAFVLGLPGETRETIKETIAYAIDLNLTSIQVSLASPYPGTEFYDMALKEGWIASDSFLD
ASGHQKCVINYPDLSNKEIFDAVELFYNKFYFRPRYIARSLFSMLVDSAQRKKLLKEGAQYLSYMRKRKQTCA

Sequences:

>Translated_473_residues
MKPLFLNPPTFEDFDGGAGARYQASREVTSFWFPGWLTYPAGMIPGARVVDAPVQRLDLDACLKIAKEFDMVVMYTSTPT
LAIDVETARRVKAQNPATVTVLTGPHVSVLPEESLRFAAGAVDIVCRGEFDYSTKELCEGKPRAEVEGISFLQDGKVVHT
KDRPPIADLDSLPFASQVYHRDLPIDEYVIPHFRHPYVSIYASRGCPSRCIYCLWPQTFSGRTLRKRSPQNVYEEVRWIK
ENLPQVKEISFDDDTFTADREHAKAIARAIKPLNVSWVINARANCDYETLKELRDAGMHHVVVGYETGNEQILKNIKKGV
TKAQAIEFTRNCHKLGLTIHGAFVLGLPGETRETIKETIAYAIDLNLTSIQVSLASPYPGTEFYDMALKEGWIASDSFLD
ASGHQKCVINYPDLSNKEIFDAVELFYNKFYFRPRYIARSLFSMLVDSAQRKKLLKEGAQYLSYMRKRKQTCA
>Mature_473_residues
MKPLFLNPPTFEDFDGGAGARYQASREVTSFWFPGWLTYPAGMIPGARVVDAPVQRLDLDACLKIAKEFDMVVMYTSTPT
LAIDVETARRVKAQNPATVTVLTGPHVSVLPEESLRFAAGAVDIVCRGEFDYSTKELCEGKPRAEVEGISFLQDGKVVHT
KDRPPIADLDSLPFASQVYHRDLPIDEYVIPHFRHPYVSIYASRGCPSRCIYCLWPQTFSGRTLRKRSPQNVYEEVRWIK
ENLPQVKEISFDDDTFTADREHAKAIARAIKPLNVSWVINARANCDYETLKELRDAGMHHVVVGYETGNEQILKNIKKGV
TKAQAIEFTRNCHKLGLTIHGAFVLGLPGETRETIKETIAYAIDLNLTSIQVSLASPYPGTEFYDMALKEGWIASDSFLD
ASGHQKCVINYPDLSNKEIFDAVELFYNKFYFRPRYIARSLFSMLVDSAQRKKLLKEGAQYLSYMRKRKQTCA

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53479; Mature: 53479

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLFLNPPTFEDFDGGAGARYQASREVTSFWFPGWLTYPAGMIPGARVVDAPVQRLDLD
CCCCCCCCCCCCCCCCCCCCCEECCCCHHHEECCCHHHCCCCCCCCCHHHHHHHHHCCHH
ACLKIAKEFDMVVMYTSTPTLAIDVETARRVKAQNPATVTVLTGPHVSVLPEESLRFAAG
HHHHHHHHCCEEEEEECCCEEEEECHHHHHHHCCCCCEEEEEECCCEEECCHHHHHHHCC
AVDIVCRGEFDYSTKELCEGKPRAEVEGISFLQDGKVVHTKDRPPIADLDSLPFASQVYH
CEEEEEECCCCCCHHHHHCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCCCHHHHHH
RDLPIDEYVIPHFRHPYVSIYASRGCPSRCIYCLWPQTFSGRTLRKRSPQNVYEEVRWIK
CCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCHHCCCHHHHHHHHHHHH
ENLPQVKEISFDDDTFTADREHAKAIARAIKPLNVSWVINARANCDYETLKELRDAGMHH
HCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCEE
VVVGYETGNEQILKNIKKGVTKAQAIEFTRNCHKLGLTIHGAFVLGLPGETRETIKETIA
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHH
YAIDLNLTSIQVSLASPYPGTEFYDMALKEGWIASDSFLDASGHQKCVINYPDLSNKEIF
HHEEEEEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHH
DAVELFYNKFYFRPRYIARSLFSMLVDSAQRKKLLKEGAQYLSYMRKRKQTCA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPLFLNPPTFEDFDGGAGARYQASREVTSFWFPGWLTYPAGMIPGARVVDAPVQRLDLD
CCCCCCCCCCCCCCCCCCCCCEECCCCHHHEECCCHHHCCCCCCCCCHHHHHHHHHCCHH
ACLKIAKEFDMVVMYTSTPTLAIDVETARRVKAQNPATVTVLTGPHVSVLPEESLRFAAG
HHHHHHHHCCEEEEEECCCEEEEECHHHHHHHCCCCCEEEEEECCCEEECCHHHHHHHCC
AVDIVCRGEFDYSTKELCEGKPRAEVEGISFLQDGKVVHTKDRPPIADLDSLPFASQVYH
CEEEEEECCCCCCHHHHHCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCCCCCHHHHHH
RDLPIDEYVIPHFRHPYVSIYASRGCPSRCIYCLWPQTFSGRTLRKRSPQNVYEEVRWIK
CCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCHHCCCHHHHHHHHHHHH
ENLPQVKEISFDDDTFTADREHAKAIARAIKPLNVSWVINARANCDYETLKELRDAGMHH
HCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCEE
VVVGYETGNEQILKNIKKGVTKAQAIEFTRNCHKLGLTIHGAFVLGLPGETRETIKETIA
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHH
YAIDLNLTSIQVSLASPYPGTEFYDMALKEGWIASDSFLDASGHQKCVINYPDLSNKEIF
HHEEEEEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHH
DAVELFYNKFYFRPRYIARSLFSMLVDSAQRKKLLKEGAQYLSYMRKRKQTCA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]