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The map label for this gene is ydaP [H]

Identifier: 197116687

GI number: 197116687

Start: 347111

End: 348904

Strand: Direct

Name: ydaP [H]

Synonym: Gbem_0287

Alternate gene names: 197116687

Gene position: 347111-348904 (Clockwise)

Preceding gene: 197116686

Following gene: 197116688

Centisome position: 7.52

GC content: 65.38

Gene sequence:

>1794_bases
ATGGCAACGACAACGAGCGATTACCTGGTGCAGCGGCTTTACGATTGGGGGGTGCGGCGGGTGTACGGCTATCCTGGCGA
CGGGATCAACGGCGTCATGGCCGCTCTGCGCAAGCAGGTGGAGCGCGTCGAGTTCATCCAGGCGCGCCACGAGGAGGAGG
CGGCCTTCCTCGCCTGCGCCCATGCCAAGTTCACCGGCGAGGTCGGGATCTGCATCGCGACTTCCGGACCGGGCGCCATA
CATCTCTTAAACGGCCTCTATGACGCGAAACTCGACCACCAGCCGGTGGTGGCAATCGTGGGGCAGCAGGGGACCACGGC
GCTGGGGTCCGATTACCAGCAGGAGGTTGACCTCATCTCGCTTTTCAAGGACGTCGCGCACCACTACGTGCACATGGCGA
GCGATCCTTCCCAGATCCGCCACCTGATCGACCGGGCCATCCGGATCGCCCTCGCGGAGCGGACCGTGACCTGCGTCATC
CTCCCCGACAACGTGCAGGAGATGGAGGCGGTGGAGTCGCCGGAGCGAAAACACGGCACCACCTTCACCGGCCTTGGCTT
CAGCCGGCCCGACGTGACGCCTGCGCGCGACGACCTGGCACGGGCGGCGGAGGTCTTGAACGCGGGCAAGAAAGTCGCGA
TGCTGGTAGGCGCCGGGGCGCTGGGCGCTTCGGACGAAGTTGCCATCGCCGCCGACATCCTCGGTGCCGGAGTCGCCAAG
GCGCTCCTAGGCAAAGCGGTCGTCCCGGACGATCTCCCCTTCGTCACAGGCTCCATCGGGCTTTTGGGTACGAAGCCCAG
CTGGGAGATGATGATGGAGTGCGACACGCTCCTCATGGTCGGCAGCGGCTTCCCGTACTCCGAGTTCCTCCCCAAGGAAG
GGCAGGCGCGCGGCGTGCAAATCGACATCAGCCCGCGCATGCTGGGGCTGCGCTACCCCATGGAGGTGAACCTGCAGGGG
GATAGTCTCCTTACCCTGAGGGCGCTGATTCCGCTATTGGAAAGAAAAAGCGACCGCGGCTGGCGGCAGGGGATAGAGAA
GAACGTCCGCGAGTGGTGGGAGATCATGCAGGCGCGCGCCATGCTCTCGGCCAACCCGATCAACCCGCAGCGCCTTTTCT
GGGAGCTCTCCTCGCGCCTCCCCGACAACTGCATCCTCACCTGCGACTCGGGCTCGGCGGCCAACTGGTACGCCCGCGAC
GTTCGCATCCGCTCGGGAATGATGGCGTCTCTGTCCGGCGGGCTCGCCACCATGTGCCCCGGCGTCCCCTATGCGACGGC
GGCGAAGATAAACCACCCGGACCGCACGGTCGTCGCCATGGTGGGAGACGGCGCCATGCAGATGCTCGGCATCAACGGGC
TCGTCACCATCTCCAAGAACTGGAAGAGCTGGAGCAACCCGCGCCTGGCGATCCTGGTTCTGGACAACAAGGACCTGAAC
CAGGTTACCTGGGAGCAGCGGGTCATGAGCGGCGACCGCAAGTTCAGCGGCTCCCAGGACATCCCTCCCTTCCCGTACGC
GCGCTACGCGGAGATGCTGGGGTTGCAGGGGATCGAGCTGGACGACCCGCAACTGATCGGGGCCGCCTGGGATCACGCAC
TGAACGCGGACCGGCCGGTGGTTATCGACGCGCATTGCGACCCGGACGTGCCGCCCCTTCCTCCGCACATCACCTTCGAG
CAGGCCAAGGGCTTCATGTTCTCCCTGGCCAAGGGGGACCCCAACCTAGGCGGCATCATCAGCCAATCGGTGAAGCAGAT
GATGTCGACCCTGCTGCCGCGAAGAGAAGAGTAG

Upstream 100 bases:

>100_bases
AATTAGCGTCTGTTAATAAATACGAGCAGGAGCTAGCGACCCTTTCGCACCCTTTAGCTATACCGAGGCCTTGCGAAACG
GGACCGCACATGGAGGCGTC

Downstream 100 bases:

>100_bases
CCGACGGGGAGGCGCCATGGCAGCGGATGCGAAGATAGAGCAGGTTGAAGCGCAGGCCTACAGGATCCCGACCGAAGACC
CGGAAAGCGACGGAAGTTAC

Product: pyruvate decarboxylase

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MATTTSDYLVQRLYDWGVRRVYGYPGDGINGVMAALRKQVERVEFIQARHEEEAAFLACAHAKFTGEVGICIATSGPGAI
HLLNGLYDAKLDHQPVVAIVGQQGTTALGSDYQQEVDLISLFKDVAHHYVHMASDPSQIRHLIDRAIRIALAERTVTCVI
LPDNVQEMEAVESPERKHGTTFTGLGFSRPDVTPARDDLARAAEVLNAGKKVAMLVGAGALGASDEVAIAADILGAGVAK
ALLGKAVVPDDLPFVTGSIGLLGTKPSWEMMMECDTLLMVGSGFPYSEFLPKEGQARGVQIDISPRMLGLRYPMEVNLQG
DSLLTLRALIPLLERKSDRGWRQGIEKNVREWWEIMQARAMLSANPINPQRLFWELSSRLPDNCILTCDSGSAANWYARD
VRIRSGMMASLSGGLATMCPGVPYATAAKINHPDRTVVAMVGDGAMQMLGINGLVTISKNWKSWSNPRLAILVLDNKDLN
QVTWEQRVMSGDRKFSGSQDIPPFPYARYAEMLGLQGIELDDPQLIGAAWDHALNADRPVVIDAHCDPDVPPLPPHITFE
QAKGFMFSLAKGDPNLGGIISQSVKQMMSTLLPRREE

Sequences:

>Translated_597_residues
MATTTSDYLVQRLYDWGVRRVYGYPGDGINGVMAALRKQVERVEFIQARHEEEAAFLACAHAKFTGEVGICIATSGPGAI
HLLNGLYDAKLDHQPVVAIVGQQGTTALGSDYQQEVDLISLFKDVAHHYVHMASDPSQIRHLIDRAIRIALAERTVTCVI
LPDNVQEMEAVESPERKHGTTFTGLGFSRPDVTPARDDLARAAEVLNAGKKVAMLVGAGALGASDEVAIAADILGAGVAK
ALLGKAVVPDDLPFVTGSIGLLGTKPSWEMMMECDTLLMVGSGFPYSEFLPKEGQARGVQIDISPRMLGLRYPMEVNLQG
DSLLTLRALIPLLERKSDRGWRQGIEKNVREWWEIMQARAMLSANPINPQRLFWELSSRLPDNCILTCDSGSAANWYARD
VRIRSGMMASLSGGLATMCPGVPYATAAKINHPDRTVVAMVGDGAMQMLGINGLVTISKNWKSWSNPRLAILVLDNKDLN
QVTWEQRVMSGDRKFSGSQDIPPFPYARYAEMLGLQGIELDDPQLIGAAWDHALNADRPVVIDAHCDPDVPPLPPHITFE
QAKGFMFSLAKGDPNLGGIISQSVKQMMSTLLPRREE
>Mature_596_residues
ATTTSDYLVQRLYDWGVRRVYGYPGDGINGVMAALRKQVERVEFIQARHEEEAAFLACAHAKFTGEVGICIATSGPGAIH
LLNGLYDAKLDHQPVVAIVGQQGTTALGSDYQQEVDLISLFKDVAHHYVHMASDPSQIRHLIDRAIRIALAERTVTCVIL
PDNVQEMEAVESPERKHGTTFTGLGFSRPDVTPARDDLARAAEVLNAGKKVAMLVGAGALGASDEVAIAADILGAGVAKA
LLGKAVVPDDLPFVTGSIGLLGTKPSWEMMMECDTLLMVGSGFPYSEFLPKEGQARGVQIDISPRMLGLRYPMEVNLQGD
SLLTLRALIPLLERKSDRGWRQGIEKNVREWWEIMQARAMLSANPINPQRLFWELSSRLPDNCILTCDSGSAANWYARDV
RIRSGMMASLSGGLATMCPGVPYATAAKINHPDRTVVAMVGDGAMQMLGINGLVTISKNWKSWSNPRLAILVLDNKDLNQ
VTWEQRVMSGDRKFSGSQDIPPFPYARYAEMLGLQGIELDDPQLIGAAWDHALNADRPVVIDAHCDPDVPPLPPHITFEQ
AKGFMFSLAKGDPNLGGIISQSVKQMMSTLLPRREE

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=590, Percent_Identity=21.6949152542373, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1787096, Length=567, Percent_Identity=33.3333333333333, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1790104, Length=570, Percent_Identity=27.5438596491228, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI87081685, Length=569, Percent_Identity=28.1195079086116, Blast_Score=172, Evalue=8e-44,
Organism=Escherichia coli, GI1786717, Length=544, Percent_Identity=24.0808823529412, Blast_Score=108, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17531299, Length=574, Percent_Identity=23.6933797909408, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17531301, Length=574, Percent_Identity=23.6933797909408, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323755, Length=575, Percent_Identity=25.2173913043478, Blast_Score=143, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6320816, Length=463, Percent_Identity=22.6781857451404, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922626, Length=579, Percent_Identity=23.6614853195164, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 65176; Mature: 65045

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTTSDYLVQRLYDWGVRRVYGYPGDGINGVMAALRKQVERVEFIQARHEEEAAFLACA
CCCCHHHHHHHHHHHHCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEE
HAKFTGEVGICIATSGPGAIHLLNGLYDAKLDHQPVVAIVGQQGTTALGSDYQQEVDLIS
CCEECCCEEEEEEECCCCCEEHHHHHHHCCCCCCCEEEEECCCCCCHHCCCHHHHHHHHH
LFKDVAHHYVHMASDPSQIRHLIDRAIRIALAERTVTCVILPDNVQEMEAVESPERKHGT
HHHHHHHHHEECCCCHHHHHHHHHHHHHHHEECCEEEEEEECCCHHHHHHHCCCCHHCCC
TFTGLGFSRPDVTPARDDLARAAEVLNAGKKVAMLVGAGALGASDEVAIAADILGAGVAK
EEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
ALLGKAVVPDDLPFVTGSIGLLGTKPSWEMMMECDTLLMVGSGFPYSEFLPKEGQARGVQ
HHHCCCCCCCCCCEEECCCEEEECCCCHHHHHHCCEEEEEECCCCHHHCCCCCCCCCCEE
IDISPRMLGLRYPMEVNLQGDSLLTLRALIPLLERKSDRGWRQGIEKNVREWWEIMQARA
EEECCCEEEEECCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
MLSANPINPQRLFWELSSRLPDNCILTCDSGSAANWYARDVRIRSGMMASLSGGLATMCP
HHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHEEHHCCCHHHHCCCHHHHCC
GVPYATAAKINHPDRTVVAMVGDGAMQMLGINGLVTISKNWKSWSNPRLAILVLDNKDLN
CCCCCCEEECCCCCCEEEEEECCCCEEEECCCEEEEEECCCCCCCCCEEEEEEECCCCCC
QVTWEQRVMSGDRKFSGSQDIPPFPYARYAEMLGLQGIELDDPQLIGAAWDHALNADRPV
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEHHHHHHCCCCCCCE
VIDAHCDPDVPPLPPHITFEQAKGFMFSLAKGDPNLGGIISQSVKQMMSTLLPRREE
EEECCCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ATTTSDYLVQRLYDWGVRRVYGYPGDGINGVMAALRKQVERVEFIQARHEEEAAFLACA
CCCHHHHHHHHHHHHCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEE
HAKFTGEVGICIATSGPGAIHLLNGLYDAKLDHQPVVAIVGQQGTTALGSDYQQEVDLIS
CCEECCCEEEEEEECCCCCEEHHHHHHHCCCCCCCEEEEECCCCCCHHCCCHHHHHHHHH
LFKDVAHHYVHMASDPSQIRHLIDRAIRIALAERTVTCVILPDNVQEMEAVESPERKHGT
HHHHHHHHHEECCCCHHHHHHHHHHHHHHHEECCEEEEEEECCCHHHHHHHCCCCHHCCC
TFTGLGFSRPDVTPARDDLARAAEVLNAGKKVAMLVGAGALGASDEVAIAADILGAGVAK
EEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
ALLGKAVVPDDLPFVTGSIGLLGTKPSWEMMMECDTLLMVGSGFPYSEFLPKEGQARGVQ
HHHCCCCCCCCCCEEECCCEEEECCCCHHHHHHCCEEEEEECCCCHHHCCCCCCCCCCEE
IDISPRMLGLRYPMEVNLQGDSLLTLRALIPLLERKSDRGWRQGIEKNVREWWEIMQARA
EEECCCEEEEECCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
MLSANPINPQRLFWELSSRLPDNCILTCDSGSAANWYARDVRIRSGMMASLSGGLATMCP
HHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHEEHHCCCHHHHCCCHHHHCC
GVPYATAAKINHPDRTVVAMVGDGAMQMLGINGLVTISKNWKSWSNPRLAILVLDNKDLN
CCCCCCEEECCCCCCEEEEEECCCCEEEECCCEEEEEECCCCCCCCCEEEEEEECCCCCC
QVTWEQRVMSGDRKFSGSQDIPPFPYARYAEMLGLQGIELDDPQLIGAAWDHALNADRPV
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEHHHHHHCCCCCCCE
VIDAHCDPDVPPLPPHITFEQAKGFMFSLAKGDPNLGGIISQSVKQMMSTLLPRREE
EEECCCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0022 {lysolecithin}} 0.0017 {lecithin}} 0.03 {sodium} 2 {ferricyanide}} 0.0012 {thiamine} 11 {pyruvate}} 10 {pyruvate}} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]