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The map label for this gene is cspLA [H]

Identifier: 197116608

GI number: 197116608

Start: 244053

End: 244253

Strand: Reverse

Name: cspLA [H]

Synonym: Gbem_0208

Alternate gene names: 197116608

Gene position: 244253-244053 (Counterclockwise)

Preceding gene: 197116609

Following gene: 197116606

Centisome position: 5.29

GC content: 54.73

Gene sequence:

>201_bases
ATGGCTAAAGGCGTAGTGAAATGGTTCAATGACAGCAAGGGGTTTGGTTTCATCGAGCAGGAGAACGGCGAAGATGTCTT
CGTGCATTTTTCCGCGATCCAGGGCGACGGGTTCAAATCCTTGGCAGAGGGCGATTCCGTCACCTTCGACGTCCAGCAGG
GTCCCAAAGGCCTGCAGGCCGCCAATGTGACCAGGGTTTAA

Upstream 100 bases:

>100_bases
AAGAGCGCTTGACACCGGTAAAAGCGCTGCATAAAATCTTCACCTGTGCCGGGGCCGGTCCCGGCAGATCAACCGTAGAG
CAGCAACAGGAGATGTGGTA

Downstream 100 bases:

>100_bases
AGGAAGCTTTAAACGTGAAAAAGCCCCCGGAGCTTAGAGCTCCGGGGGCTTTTTTTATTTTGCGGGGACTAATGGTCCCG
GCGCTACTCGATGCAGCTTC

Product: cold shock DNA/RNA-binding protein

Products: NA

Alternate protein names: CspL [H]

Number of amino acids: Translated: 66; Mature: 65

Protein sequence:

>66_residues
MAKGVVKWFNDSKGFGFIEQENGEDVFVHFSAIQGDGFKSLAEGDSVTFDVQQGPKGLQAANVTRV

Sequences:

>Translated_66_residues
MAKGVVKWFNDSKGFGFIEQENGEDVFVHFSAIQGDGFKSLAEGDSVTFDVQQGPKGLQAANVTRV
>Mature_65_residues
AKGVVKWFNDSKGFGFIEQENGEDVFVHFSAIQGDGFKSLAEGDSVTFDVQQGPKGLQAANVTRV

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1788126, Length=65, Percent_Identity=66.1538461538461, Blast_Score=84, Evalue=1e-18,
Organism=Escherichia coli, GI1786841, Length=62, Percent_Identity=67.741935483871, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=58.2089552238806, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=60.9375, Blast_Score=79, Evalue=6e-17,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=60.655737704918, Blast_Score=74, Evalue=1e-15,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=54.6875, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=53.125, Blast_Score=70, Evalue=3e-14,

Paralogues:

None

Copy number: 8300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7137; Mature: 7006

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGVVKWFNDSKGFGFIEQENGEDVFVHFSAIQGDGFKSLAEGDSVTFDVQQGPKGLQA
CCCCCEEECCCCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCC
ANVTRV
CCEECC
>Mature Secondary Structure 
AKGVVKWFNDSKGFGFIEQENGEDVFVHFSAIQGDGFKSLAEGDSVTFDVQQGPKGLQA
CCCCEEECCCCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCC
ANVTRV
CCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669; 9439003 [H]