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The map label for this gene is ligT [C]

Identifier: 197116507

GI number: 197116507

Start: 129392

End: 129946

Strand: Reverse

Name: ligT [C]

Synonym: Gbem_0107

Alternate gene names: 197116507

Gene position: 129946-129392 (Counterclockwise)

Preceding gene: 197116514

Following gene: 197116501

Centisome position: 2.82

GC content: 62.34

Gene sequence:

>555_bases
TTGCCAAGACTCTTCGTTGCCATCGATCTGCCAGAGGAAATCAAAGCCTCTCTCTCACAGCTTTCCTGCGCTGTGACAGG
CGCCAGGTGGGTCGCTCCTTCCGAGATCCATCTCACCTTGCGCTTCATAGGAGACGTCGACCCTCAGACAGTTTCCAAGA
TAGAGAAGGCCTTATCCGCGGTGCAGTTCCCCTCTTTTCCCCTCCGCGTAGCCGGGGTCGGCCACTTCCCTGCGCGCGGC
TATCCCCGCGTGCTTTGGGTCGGGCTGGAACCGCACCCCGAGCTCATCGCCCTGCAGCAGCGGATCGAATCCGCGCTGCA
ACCGGCAGGTGTTTCACCGGAAGACCGCCCTTTTTCCCCGCACATAACGCTCGCCCGCTTGAAAGAGACGCCCCCTGCTG
CGGTCGCCGCCTTCGAGTCGTTGCACCGCGGGCTCGCCTACCCCCCTTTCCCCGCAACCGAGTACGTCCTCTACTCCAGC
GTCCTCACCCCCAAAGGCGCCATCCACCGCAAGGAAGAGGTCTTCCGTTCAAGCGAAGAAGCGGCTTCACGGTGA

Upstream 100 bases:

>100_bases
CAGATCACCAAAGCTATACTGTCTAACACTCAAATTTCAGTTAGTCAACCGTTTTTTGCACTCCCAGATACTCTTCCTGC
ATTTCCACGAGGAGGCCACC

Downstream 100 bases:

>100_bases
AGGCGGGACTGCCGCACGTGCCGCGAACGGCGCAGGGCCAGTGAGATTCCCCGCGAAGACGGCAGGACCGAGCCAGGCCG
TTTTGGCCTTGTGAGCTTGA

Product: 2',5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MPRLFVAIDLPEEIKASLSQLSCAVTGARWVAPSEIHLTLRFIGDVDPQTVSKIEKALSAVQFPSFPLRVAGVGHFPARG
YPRVLWVGLEPHPELIALQQRIESALQPAGVSPEDRPFSPHITLARLKETPPAAVAAFESLHRGLAYPPFPATEYVLYSS
VLTPKGAIHRKEEVFRSSEEAASR

Sequences:

>Translated_184_residues
MPRLFVAIDLPEEIKASLSQLSCAVTGARWVAPSEIHLTLRFIGDVDPQTVSKIEKALSAVQFPSFPLRVAGVGHFPARG
YPRVLWVGLEPHPELIALQQRIESALQPAGVSPEDRPFSPHITLARLKETPPAAVAAFESLHRGLAYPPFPATEYVLYSS
VLTPKGAIHRKEEVFRSSEEAASR
>Mature_183_residues
PRLFVAIDLPEEIKASLSQLSCAVTGARWVAPSEIHLTLRFIGDVDPQTVSKIEKALSAVQFPSFPLRVAGVGHFPARGY
PRVLWVGLEPHPELIALQQRIESALQPAGVSPEDRPFSPHITLARLKETPPAAVAAFESLHRGLAYPPFPATEYVLYSSV
LTPKGAIHRKEEVFRSSEEAASR

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

Organism=Escherichia coli, GI87081692, Length=128, Percent_Identity=33.59375, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20166; Mature: 20035

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLFVAIDLPEEIKASLSQLSCAVTGARWVAPSEIHLTLRFIGDVDPQTVSKIEKALSA
CCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
VQFPSFPLRVAGVGHFPARGYPRVLWVGLEPHPELIALQQRIESALQPAGVSPEDRPFSP
HCCCCCCEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
HITLARLKETPPAAVAAFESLHRGLAYPPFPATEYVLYSSVLTPKGAIHRKEEVFRSSEE
CEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCHH
AASR
HHCC
>Mature Secondary Structure 
PRLFVAIDLPEEIKASLSQLSCAVTGARWVAPSEIHLTLRFIGDVDPQTVSKIEKALSA
CEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
VQFPSFPLRVAGVGHFPARGYPRVLWVGLEPHPELIALQQRIESALQPAGVSPEDRPFSP
HCCCCCCEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
HITLARLKETPPAAVAAFESLHRGLAYPPFPATEYVLYSSVLTPKGAIHRKEEVFRSSEE
CEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCHH
AASR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]