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The map label for this gene is lcfB [H]

Identifier: 197106179

GI number: 197106179

Start: 3112742

End: 3114280

Strand: Direct

Name: lcfB [H]

Synonym: PHZ_c2718

Alternate gene names: 197106179

Gene position: 3112742-3114280 (Clockwise)

Preceding gene: 197106175

Following gene: 197106181

Centisome position: 77.89

GC content: 67.51

Gene sequence:

>1539_bases
ATGCATCCGGCGATCCACGCCAAGACCCAGCCCGACCGGGCCGCCTACGTCATGGGCTCCTCGGGCGAGACGGTGACCTA
CCGCGAGCTGGACGAGCGCTCGAACCAGGGGGCGCACCTGTTCCGCAGCCTGGGCCTGAAGACCGGCGACGTGATCGCGA
TCTTCATGGACAACAATCCGCGCTACTTCGAGATCGCCTGGGCCGCCCAGCGCTCGGGCCTCTACTACACCTGCATCTCG
TCCAAGCTGACCGCGGCCGAGGTCGAGTACATCGTGGGCGACTGCAGCGCCAAGGTGCTGATCGCCTCGCCGGGCGTGGG
GCCGGTCGTGGACGAACTGCCCGCCGTGCTGAAGGGCGTGAAGCTGTTCATGACCGGCGAGGCCCGCCCGCCCTACGAGA
GCTTCGAGGCCGCGCGGGCGGAGTTCCCGACGAGCCCGATCGCTGACGAAACCGCCGGCTCGGACATGCTCTATTCGTCG
GGCACCACCGGCCGACCCAAGGGGATCAAGCCGCCGCTGACCGGCCTGCCGATCGACGCGCCGAACGCCCTGCAGATGAT
GGCGCAAGGCCTGTTCGGCTTCCAAGAGGGCTGCACCTACATCTCGCCGGCGCCGCTGTACCACGCCGCCCCGCTGCGCT
GGTGCATGACCGTCCACAAGCTGGGCGGGACGGTGATCGTGATGGAGAAATTCGATCCCGAGCACGCCCTGGCGCTGATC
GAGAAGCACAAGGCCGACTGCGGCCAGTTCGTGCCGACCCACTTCGTGCGCATGCTGAAGCTGCCGGAGGAGGTGCGGGC
GAAGTACGACGTCAGCTCGATGAAGTCGGCGGTGCACGCCGCCGCCCCCTGCCCCATCCCCGTGAAGGAACAGATGATCG
CCTGGTGGGGCCCGGTGATCTACGAGTACTACGCCGGCACCGAGGGCAACGGCTTCTGCTTCATCAACTCGCACGACTGG
CTGACCCACAAGGGCTCGGTCGGCAAGGCGGTGCTGGGCGAGCTGAAGATCTGCGGCGAGGACGGCGACGAGTTGCCGCC
GCGTAGCGAGGGCGTGGTCCACTTCGCCAACGGCCCGCCGCTCAGCTACCACAACGCCCCCGAGAAGGTCGCCGAGGGCA
CCAACAAGCACGGCTGGACGACGCTGGGCGACGTGGGCTGGATGGACGAAGAGGGCTACCTCTACCTCACCGACCGCAAG
AGCTTCATGATCATCTCGGGCGGCGTGAACATCTATCCGCAGGAGATCGAGAACCTGCTGGTCACCCATCCCAAGGTCGC
GGACGCCGCCGTCGTCGGCGCGCCCGACGAGGAGATGGGCGAGCGGGTCGTGGCCGTCATCCAGCCGATGGACTGGACCG
CCGATCACGAAGCCCTGCGGGCCGAACTGCTGGCCTACACGCGCCAGAACCTCAGCCACGTGAAGTCGCCGCGGGTCATC
GACTTCATGCAGGAGCTGCCCCGGCACCCCACCGGCAAGCTCTACAAGCGCCTGATCCGCGACGCCTACTGGGGCAAGGA
AGGCTCGCGCATCGTCTGA

Upstream 100 bases:

>100_bases
GGCCATACCGGCCCGCTTGACGGACCGTAGGTCAAGGCCCGCATTTCCCGGAAAGAGAAGCACATGTCGGCGGGACCAAC
CCGCCCGCGGGAGTGAAAGC

Downstream 100 bases:

>100_bases
GGCGCCTATGCCCGGCGGCGCTCGTCCGCCGGAGCCACGACCGCCGGGGCGGCCGCCGCCGGCGCGGCCGACAGCAGGGC
GTCCAGCCGCTCGACCAGCA

Product: long-chain-fatty-acid--CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MHPAIHAKTQPDRAAYVMGSSGETVTYRELDERSNQGAHLFRSLGLKTGDVIAIFMDNNPRYFEIAWAAQRSGLYYTCIS
SKLTAAEVEYIVGDCSAKVLIASPGVGPVVDELPAVLKGVKLFMTGEARPPYESFEAARAEFPTSPIADETAGSDMLYSS
GTTGRPKGIKPPLTGLPIDAPNALQMMAQGLFGFQEGCTYISPAPLYHAAPLRWCMTVHKLGGTVIVMEKFDPEHALALI
EKHKADCGQFVPTHFVRMLKLPEEVRAKYDVSSMKSAVHAAAPCPIPVKEQMIAWWGPVIYEYYAGTEGNGFCFINSHDW
LTHKGSVGKAVLGELKICGEDGDELPPRSEGVVHFANGPPLSYHNAPEKVAEGTNKHGWTTLGDVGWMDEEGYLYLTDRK
SFMIISGGVNIYPQEIENLLVTHPKVADAAVVGAPDEEMGERVVAVIQPMDWTADHEALRAELLAYTRQNLSHVKSPRVI
DFMQELPRHPTGKLYKRLIRDAYWGKEGSRIV

Sequences:

>Translated_512_residues
MHPAIHAKTQPDRAAYVMGSSGETVTYRELDERSNQGAHLFRSLGLKTGDVIAIFMDNNPRYFEIAWAAQRSGLYYTCIS
SKLTAAEVEYIVGDCSAKVLIASPGVGPVVDELPAVLKGVKLFMTGEARPPYESFEAARAEFPTSPIADETAGSDMLYSS
GTTGRPKGIKPPLTGLPIDAPNALQMMAQGLFGFQEGCTYISPAPLYHAAPLRWCMTVHKLGGTVIVMEKFDPEHALALI
EKHKADCGQFVPTHFVRMLKLPEEVRAKYDVSSMKSAVHAAAPCPIPVKEQMIAWWGPVIYEYYAGTEGNGFCFINSHDW
LTHKGSVGKAVLGELKICGEDGDELPPRSEGVVHFANGPPLSYHNAPEKVAEGTNKHGWTTLGDVGWMDEEGYLYLTDRK
SFMIISGGVNIYPQEIENLLVTHPKVADAAVVGAPDEEMGERVVAVIQPMDWTADHEALRAELLAYTRQNLSHVKSPRVI
DFMQELPRHPTGKLYKRLIRDAYWGKEGSRIV
>Mature_512_residues
MHPAIHAKTQPDRAAYVMGSSGETVTYRELDERSNQGAHLFRSLGLKTGDVIAIFMDNNPRYFEIAWAAQRSGLYYTCIS
SKLTAAEVEYIVGDCSAKVLIASPGVGPVVDELPAVLKGVKLFMTGEARPPYESFEAARAEFPTSPIADETAGSDMLYSS
GTTGRPKGIKPPLTGLPIDAPNALQMMAQGLFGFQEGCTYISPAPLYHAAPLRWCMTVHKLGGTVIVMEKFDPEHALALI
EKHKADCGQFVPTHFVRMLKLPEEVRAKYDVSSMKSAVHAAAPCPIPVKEQMIAWWGPVIYEYYAGTEGNGFCFINSHDW
LTHKGSVGKAVLGELKICGEDGDELPPRSEGVVHFANGPPLSYHNAPEKVAEGTNKHGWTTLGDVGWMDEEGYLYLTDRK
SFMIISGGVNIYPQEIENLLVTHPKVADAAVVGAPDEEMGERVVAVIQPMDWTADHEALRAELLAYTRQNLSHVKSPRVI
DFMQELPRHPTGKLYKRLIRDAYWGKEGSRIV

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=518, Percent_Identity=27.4131274131274, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI156151445, Length=556, Percent_Identity=26.6187050359712, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI122937307, Length=524, Percent_Identity=27.8625954198473, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI157311624, Length=530, Percent_Identity=26.4150943396226, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI157311622, Length=530, Percent_Identity=26.4150943396226, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI58082049, Length=529, Percent_Identity=26.6540642722117, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI42544132, Length=518, Percent_Identity=26.2548262548263, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI115511026, Length=542, Percent_Identity=24.7232472324723, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI187761345, Length=527, Percent_Identity=25.6166982922201, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI187761343, Length=527, Percent_Identity=25.6166982922201, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI13375727, Length=528, Percent_Identity=24.2424242424242, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI28416953, Length=540, Percent_Identity=24.6296296296296, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI38524616, Length=548, Percent_Identity=25.5474452554745, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI227499619, Length=472, Percent_Identity=24.1525423728814, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI62865631, Length=548, Percent_Identity=20.985401459854, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI13325055, Length=548, Percent_Identity=20.985401459854, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788107, Length=541, Percent_Identity=28.2809611829945, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI145693145, Length=512, Percent_Identity=27.34375, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI221142682, Length=523, Percent_Identity=23.5181644359465, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790505, Length=566, Percent_Identity=23.321554770318, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786810, Length=496, Percent_Identity=24.1935483870968, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=523, Percent_Identity=28.2982791586998, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17558820, Length=504, Percent_Identity=27.3809523809524, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI32563687, Length=504, Percent_Identity=25.5952380952381, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71994694, Length=534, Percent_Identity=27.3408239700375, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=530, Percent_Identity=26.4150943396226, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71994703, Length=534, Percent_Identity=27.3408239700375, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71994690, Length=534, Percent_Identity=27.3408239700375, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17559526, Length=519, Percent_Identity=27.1676300578035, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17538037, Length=525, Percent_Identity=27.2380952380952, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17560140, Length=503, Percent_Identity=25.2485089463221, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=543, Percent_Identity=25.2302025782689, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17551278, Length=554, Percent_Identity=24.7292418772563, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=361, Percent_Identity=25.4847645429363, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323182, Length=575, Percent_Identity=25.7391304347826, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=27.0194986072423, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21355181, Length=512, Percent_Identity=26.3671875, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24581924, Length=546, Percent_Identity=24.5421245421245, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648253, Length=514, Percent_Identity=25.0972762645914, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24648255, Length=456, Percent_Identity=25.4385964912281, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21358303, Length=436, Percent_Identity=24.3119266055046, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18859661, Length=528, Percent_Identity=25.5681818181818, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI21356441, Length=455, Percent_Identity=22.8571428571429, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24656500, Length=512, Percent_Identity=22.4609375, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648260, Length=518, Percent_Identity=23.3590733590734, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI20130357, Length=518, Percent_Identity=25.0965250965251, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922652, Length=525, Percent_Identity=22.8571428571429, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161076582, Length=539, Percent_Identity=23.0055658627087, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI62472339, Length=552, Percent_Identity=23.0072463768116, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24667955, Length=552, Percent_Identity=23.0072463768116, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648257, Length=522, Percent_Identity=21.8390804597701, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24653035, Length=521, Percent_Identity=22.4568138195777, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21356947, Length=526, Percent_Identity=22.4334600760456, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281365686, Length=458, Percent_Identity=22.2707423580786, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355225, Length=530, Percent_Identity=22.0754716981132, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45552801, Length=548, Percent_Identity=23.5401459854015, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550496, Length=548, Percent_Identity=23.5401459854015, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24667959, Length=381, Percent_Identity=24.6719160104987, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56289; Mature: 56289

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPAIHAKTQPDRAAYVMGSSGETVTYRELDERSNQGAHLFRSLGLKTGDVIAIFMDNNP
CCCCCCCCCCCCCEEEEECCCCCEEEHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCC
RYFEIAWAAQRSGLYYTCISSKLTAAEVEYIVGDCSAKVLIASPGVGPVVDELPAVLKGV
CEEEEEEEECCCCEEEEEECCCCCHHHHHHEEECCCCEEEEECCCCCHHHHHHHHHHHCC
KLFMTGEARPPYESFEAARAEFPTSPIADETAGSDMLYSSGTTGRPKGIKPPLTGLPIDA
EEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
PNALQMMAQGLFGFQEGCTYISPAPLYHAAPLRWCMTVHKLGGTVIVMEKFDPEHALALI
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHH
EKHKADCGQFVPTHFVRMLKLPEEVRAKYDVSSMKSAVHAAAPCPIPVKEQMIAWWGPVI
HHHHHHHCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
YEYYAGTEGNGFCFINSHDWLTHKGSVGKAVLGELKICGEDGDELPPRSEGVVHFANGPP
HHHHCCCCCCEEEEECCCCCEECCCCCCHHHHCCEEEECCCCCCCCCCCCCEEEECCCCC
LSYHNAPEKVAEGTNKHGWTTLGDVGWMDEEGYLYLTDRKSFMIISGGVNIYPQEIENLL
CCCCCCHHHHHCCCCCCCCEEECCCCCCCCCCEEEEECCCEEEEEECCCEECHHHHHHHH
VTHPKVADAAVVGAPDEEMGERVVAVIQPMDWTADHEALRAELLAYTRQNLSHVKSPRVI
HCCCCHHCEEEECCCHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
DFMQELPRHPTGKLYKRLIRDAYWGKEGSRIV
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MHPAIHAKTQPDRAAYVMGSSGETVTYRELDERSNQGAHLFRSLGLKTGDVIAIFMDNNP
CCCCCCCCCCCCCEEEEECCCCCEEEHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCC
RYFEIAWAAQRSGLYYTCISSKLTAAEVEYIVGDCSAKVLIASPGVGPVVDELPAVLKGV
CEEEEEEEECCCCEEEEEECCCCCHHHHHHEEECCCCEEEEECCCCCHHHHHHHHHHHCC
KLFMTGEARPPYESFEAARAEFPTSPIADETAGSDMLYSSGTTGRPKGIKPPLTGLPIDA
EEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
PNALQMMAQGLFGFQEGCTYISPAPLYHAAPLRWCMTVHKLGGTVIVMEKFDPEHALALI
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHH
EKHKADCGQFVPTHFVRMLKLPEEVRAKYDVSSMKSAVHAAAPCPIPVKEQMIAWWGPVI
HHHHHHHCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
YEYYAGTEGNGFCFINSHDWLTHKGSVGKAVLGELKICGEDGDELPPRSEGVVHFANGPP
HHHHCCCCCCEEEEECCCCCEECCCCCCHHHHCCEEEECCCCCCCCCCCCCEEEECCCCC
LSYHNAPEKVAEGTNKHGWTTLGDVGWMDEEGYLYLTDRKSFMIISGGVNIYPQEIENLL
CCCCCCHHHHHCCCCCCCCEEECCCCCCCCCCEEEEECCCEEEEEECCCEECHHHHHHHH
VTHPKVADAAVVGAPDEEMGERVVAVIQPMDWTADHEALRAELLAYTRQNLSHVKSPRVI
HCCCCHHCEEEECCCHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
DFMQELPRHPTGKLYKRLIRDAYWGKEGSRIV
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]