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The map label for this gene is gatA [H]

Identifier: 197106134

GI number: 197106134

Start: 3062803

End: 3064290

Strand: Direct

Name: gatA [H]

Synonym: PHZ_c2673

Alternate gene names: 197106134

Gene position: 3062803-3064290 (Clockwise)

Preceding gene: 197106133

Following gene: 197106135

Centisome position: 76.64

GC content: 74.4

Gene sequence:

>1488_bases
ATGCGCGACGGCCGGGCCACCGCCCACGCCCTCGTCCGCGCCTACTTCGACCGGATCGCCGCGGTGGACGATGCGGGCCC
GAAACTCAACAGCGTCATCGCCCTCAACCCGCAGGCCCTGGCCGACGCCGAGCGCCTGGACGCCGAGCGCCGCGCCGGCC
GCACGCGCGGGCCGCTGCACGGGATCACCGTGCTGCTGAAGGACAACATCGAGGCCGACATCGGCCTGCCGACCACCGCC
GGCTCCCTGGCTCTGGCCGACAACGTGACGGGCCGCGACGCCCCGATCGTGAAACGCCTGCGCGACGCCGGCGCGATCGT
GCTGGGCAAGGCGAACCTCTCCGAATGGGCCAACTTCCGCTCCACCCGCTCGATCAGCGGCTGGAGCGCCGTGGGCGGGC
TCACGCGCAACCCCTACGGCCTCGACCGCACGGCCTGCGGTTCAAGCTCGGGCTCGGGCGCGGCGGTGGCTGCGGGGCTC
GCCGCGGCGGCCATCGGCACCGAGACCGACGGCTCCATCACCTGCCCGGCGGCGATGACCGGGATCGTCGGCCTGAAGCC
CACCGTGGGCCTCGTCTCGCGCGCGCTGATCGTGCCGATCAGCCCCGCCCAGGACACCGCCGGCCCGATGACCCGCACGG
TCGCCGACGCCGCGGCGGTGCTGCAGGTGATCGCCGGCTCCGACCCCGCCGATCCGGCGACGGCCGAGGCCGACGCGAAG
AAGGTCGACTACCTGGGGGCCCTCGACCGCGACGCCCTGAAGGGCGCCCGCATCGGCGTCTGGCACGGCTTCAAGGGCCG
GCATGCGGCGCTGGATGAGGCGTTCGAGGCCGCGCTCGCCGATCTCAAGGCCGCCGGCGCCGAGCTGGTGGAGATCAAGG
GGCCCGCCGAGGCCGAGCTCGCCCGCATCGGCGAGCTGGAAGGCGAGCTCCTGCGCTGGGAGTTCAAGGCGGCCCTCGAC
GACTACCTGGCCCGCACGCCGCCTGCGGTGAAGAGCCGGACCCTGGCCGACCTCATCGCCTTCAACCGCGCCGAGATCCG
CGAGACGCCGCTGTTCGGCCAGGAGATCTTCGAACAGGCGGTGAAGGCGCCCCCCGCCTCGGACCCGGCGATGCAGACCA
AGCGGGCCGAGGCCCGCCGGCTGGCCGCCCAGGCCCTCGACCGGATGCTGGCCGAGCAGAAGGTCCTGGCGATCGTCGCG
CCCAGCGGCGGGCCCGCCTCGATCGTCGATCCTGTGAACGGCGGGCGGTTCTTCGGCTCGCCCTCGACCCTGCCGGCGGT
CTCCGGCTATCCGCACCTGACCGTGCCGATGGGCCATGTGACGGGCCTGCCGGTGGGGATCTCCTTCCTGGGGCCGGCCT
GGTCCGAGGCGCGGCTGCTGTCGCTGGGCTTCGCCTACGAACAGGCCGCGCGGGCGCGGCGCGAACCCGGGTTCCCGCCG
GACGTGGCCGCCCGGCCCGAGATCGCCCGCGCCTACGATCCGCGCTGA

Upstream 100 bases:

>100_bases
CGTCATCGCCGCGGGCCTCGCGCTCGCCGCCACCGCCGCCGTCAAGGCCCAGGCCCAGCCGCTCAGCCCCTACGCCTCGG
CCGGCCAGCAGCAGGCGGCC

Downstream 100 bases:

>100_bases
ACGGCCAAGCGGAACGGCCAAGCGGAACGGCCAGGCGGAACGGGCGGGCGCAACCCTCGCGCGGCCCGGCGGGTTACAGC
CGGGCGCCGGCGCCGCCGGC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MRDGRATAHALVRAYFDRIAAVDDAGPKLNSVIALNPQALADAERLDAERRAGRTRGPLHGITVLLKDNIEADIGLPTTA
GSLALADNVTGRDAPIVKRLRDAGAIVLGKANLSEWANFRSTRSISGWSAVGGLTRNPYGLDRTACGSSSGSGAAVAAGL
AAAAIGTETDGSITCPAAMTGIVGLKPTVGLVSRALIVPISPAQDTAGPMTRTVADAAAVLQVIAGSDPADPATAEADAK
KVDYLGALDRDALKGARIGVWHGFKGRHAALDEAFEAALADLKAAGAELVEIKGPAEAELARIGELEGELLRWEFKAALD
DYLARTPPAVKSRTLADLIAFNRAEIRETPLFGQEIFEQAVKAPPASDPAMQTKRAEARRLAAQALDRMLAEQKVLAIVA
PSGGPASIVDPVNGGRFFGSPSTLPAVSGYPHLTVPMGHVTGLPVGISFLGPAWSEARLLSLGFAYEQAARARREPGFPP
DVAARPEIARAYDPR

Sequences:

>Translated_495_residues
MRDGRATAHALVRAYFDRIAAVDDAGPKLNSVIALNPQALADAERLDAERRAGRTRGPLHGITVLLKDNIEADIGLPTTA
GSLALADNVTGRDAPIVKRLRDAGAIVLGKANLSEWANFRSTRSISGWSAVGGLTRNPYGLDRTACGSSSGSGAAVAAGL
AAAAIGTETDGSITCPAAMTGIVGLKPTVGLVSRALIVPISPAQDTAGPMTRTVADAAAVLQVIAGSDPADPATAEADAK
KVDYLGALDRDALKGARIGVWHGFKGRHAALDEAFEAALADLKAAGAELVEIKGPAEAELARIGELEGELLRWEFKAALD
DYLARTPPAVKSRTLADLIAFNRAEIRETPLFGQEIFEQAVKAPPASDPAMQTKRAEARRLAAQALDRMLAEQKVLAIVA
PSGGPASIVDPVNGGRFFGSPSTLPAVSGYPHLTVPMGHVTGLPVGISFLGPAWSEARLLSLGFAYEQAARARREPGFPP
DVAARPEIARAYDPR
>Mature_495_residues
MRDGRATAHALVRAYFDRIAAVDDAGPKLNSVIALNPQALADAERLDAERRAGRTRGPLHGITVLLKDNIEADIGLPTTA
GSLALADNVTGRDAPIVKRLRDAGAIVLGKANLSEWANFRSTRSISGWSAVGGLTRNPYGLDRTACGSSSGSGAAVAAGL
AAAAIGTETDGSITCPAAMTGIVGLKPTVGLVSRALIVPISPAQDTAGPMTRTVADAAAVLQVIAGSDPADPATAEADAK
KVDYLGALDRDALKGARIGVWHGFKGRHAALDEAFEAALADLKAAGAELVEIKGPAEAELARIGELEGELLRWEFKAALD
DYLARTPPAVKSRTLADLIAFNRAEIRETPLFGQEIFEQAVKAPPASDPAMQTKRAEARRLAAQALDRMLAEQKVLAIVA
PSGGPASIVDPVNGGRFFGSPSTLPAVSGYPHLTVPMGHVTGLPVGISFLGPAWSEARLLSLGFAYEQAARARREPGFPP
DVAARPEIARAYDPR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=229, Percent_Identity=37.117903930131, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI195972892, Length=238, Percent_Identity=30.2521008403361, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17556264, Length=307, Percent_Identity=27.6872964169381, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=235, Percent_Identity=28.936170212766, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=231, Percent_Identity=29.4372294372294, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=188, Percent_Identity=30.3191489361702, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=436, Percent_Identity=27.7522935779816, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323950, Length=293, Percent_Identity=26.6211604095563, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648113, Length=469, Percent_Identity=28.997867803838, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24644968, Length=496, Percent_Identity=27.2177419354839, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356731, Length=254, Percent_Identity=32.2834645669291, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648435, Length=245, Percent_Identity=30.2040816326531, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648437, Length=245, Percent_Identity=30.2040816326531, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI45550774, Length=245, Percent_Identity=30.2040816326531, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24652985, Length=243, Percent_Identity=30.8641975308642, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19922090, Length=243, Percent_Identity=30.8641975308642, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24652981, Length=243, Percent_Identity=30.8641975308642, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24652983, Length=243, Percent_Identity=30.8641975308642, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24648441, Length=180, Percent_Identity=33.8888888888889, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648439, Length=180, Percent_Identity=33.8888888888889, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI161078093, Length=325, Percent_Identity=29.8461538461538, Blast_Score=79, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 51488; Mature: 51488

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDGRATAHALVRAYFDRIAAVDDAGPKLNSVIALNPQALADAERLDAERRAGRTRGPLH
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCC
GITVLLKDNIEADIGLPTTAGSLALADNVTGRDAPIVKRLRDAGAIVLGKANLSEWANFR
CEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCEEEEECCCHHHHHHHH
STRSISGWSAVGGLTRNPYGLDRTACGSSSGSGAAVAAGLAAAAIGTETDGSITCPAAMT
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECHHHHH
GIVGLKPTVGLVSRALIVPISPAQDTAGPMTRTVADAAAVLQVIAGSDPADPATAEADAK
HCCCCCCHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
KVDYLGALDRDALKGARIGVWHGFKGRHAALDEAFEAALADLKAAGAELVEIKGPAEAEL
HHHHHCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
ARIGELEGELLRWEFKAALDDYLARTPPAVKSRTLADLIAFNRAEIRETPLFGQEIFEQA
HHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHH
VKAPPASDPAMQTKRAEARRLAAQALDRMLAEQKVLAIVAPSGGPASIVDPVNGGRFFGS
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCEECCC
PSTLPAVSGYPHLTVPMGHVTGLPVGISFLGPAWSEARLLSLGFAYEQAARARREPGFPP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DVAARPEIARAYDPR
CCCCCCCHHHCCCCC
>Mature Secondary Structure
MRDGRATAHALVRAYFDRIAAVDDAGPKLNSVIALNPQALADAERLDAERRAGRTRGPLH
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCC
GITVLLKDNIEADIGLPTTAGSLALADNVTGRDAPIVKRLRDAGAIVLGKANLSEWANFR
CEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCEEEEECCCHHHHHHHH
STRSISGWSAVGGLTRNPYGLDRTACGSSSGSGAAVAAGLAAAAIGTETDGSITCPAAMT
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECHHHHH
GIVGLKPTVGLVSRALIVPISPAQDTAGPMTRTVADAAAVLQVIAGSDPADPATAEADAK
HCCCCCCHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
KVDYLGALDRDALKGARIGVWHGFKGRHAALDEAFEAALADLKAAGAELVEIKGPAEAEL
HHHHHCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
ARIGELEGELLRWEFKAALDDYLARTPPAVKSRTLADLIAFNRAEIRETPLFGQEIFEQA
HHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHH
VKAPPASDPAMQTKRAEARRLAAQALDRMLAEQKVLAIVAPSGGPASIVDPVNGGRFFGS
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCEECCC
PSTLPAVSGYPHLTVPMGHVTGLPVGISFLGPAWSEARLLSLGFAYEQAARARREPGFPP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DVAARPEIARAYDPR
CCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA