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The map label for this gene is purU [H]

Identifier: 197105876

GI number: 197105876

Start: 2751201

End: 2752043

Strand: Direct

Name: purU [H]

Synonym: PHZ_c2415

Alternate gene names: 197105876

Gene position: 2751201-2752043 (Clockwise)

Preceding gene: 197105873

Following gene: 197105883

Centisome position: 68.84

GC content: 69.04

Gene sequence:

>843_bases
ATGATCCTGACGCTATCCTGTCCCGACCAGCCCGGCATCGTGGCCCGGGTTTCCGCCCTGCTCTTTGAGCGCGGCGCCAA
CATCCTCGACGCCCAGCAGTTCGACGACGAGGAGACCGGCCGGTTCTTCATGCGCGTGGTCTTCGACCCGGGTCAGGCCG
ACGTGGCCGCCTGGCGCGACGCCTTCGCTCCGCTCGGCGAACAGTACGGCATGACGTGGCAGATGCGCGCCCGCGGCGAG
CGCCGCCGGGTGATGATCCTGGCGTCCCAGCAGGACCACTGCCTGGCCGACCTCATCTGGCGCTGGCGCCAGGGCGAGCT
GCCGATGGACATCACCGCGGTGGTCTCCAACCACCCGGCGTCCACCTATCCGCACACCGACCTGCACGGCATCGCCTTCC
ATCACCTGCCGATCACGGCCGACACCAAGCCGCAGCAGGAAGCCCGGCTCTGGAAGCTGATCCAGGAGACCGGGACCGAG
CTCGTGGTGCTCGCCCGCTACATGCAGATCCTGTCGGACGATCTGTCGGGCAAGCTCGAGGGCCGCTGCATCAACATCCA
CCACTCGTTCCTGCCGGGCTTCAAGGGCGCCAGGCCCTACCACCAGGCGCACGCCCGCGGCGTGAAGGTGATCGGCGCGA
CCGCCCACTACGTCACCGCCGACCTCGACGAGGGTCCGATCATCGAGCAGGACGTCGAGCGCATCAGCCACCGCGACCAT
CCGCGCGACCTGGTCCGCAAGGGCCGCGACATCGAGCGCCGCGTCCTGGCCCGCGCCGTCCGCTGGCATCTGGAGGACCG
CGTGCTCCTGAACGGCCGCAAGACCGTGGTCTTCACCGACTAG

Upstream 100 bases:

>100_bases
CCCCCCAGCCCTGGCTCCGGAAGCACGGCCAGACCAACTGGACAGGGTCACCCTGTGTCAGCCTTGACCGGGCTCACTGA
GGTTGGCACGGGCGGCCTCG

Downstream 100 bases:

>100_bases
GCTGTGACGCCCGAAAGGTGAGACGCTCGCTCACGCCGGGCGACGGCGGTCAGCGCCGCGAACTCAGCGGGGCGATGTCA
TGCGGACGAAGGACGTTGTC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MILTLSCPDQPGIVARVSALLFERGANILDAQQFDDEETGRFFMRVVFDPGQADVAAWRDAFAPLGEQYGMTWQMRARGE
RRRVMILASQQDHCLADLIWRWRQGELPMDITAVVSNHPASTYPHTDLHGIAFHHLPITADTKPQQEARLWKLIQETGTE
LVVLARYMQILSDDLSGKLEGRCINIHHSFLPGFKGARPYHQAHARGVKVIGATAHYVTADLDEGPIIEQDVERISHRDH
PRDLVRKGRDIERRVLARAVRWHLEDRVLLNGRKTVVFTD

Sequences:

>Translated_280_residues
MILTLSCPDQPGIVARVSALLFERGANILDAQQFDDEETGRFFMRVVFDPGQADVAAWRDAFAPLGEQYGMTWQMRARGE
RRRVMILASQQDHCLADLIWRWRQGELPMDITAVVSNHPASTYPHTDLHGIAFHHLPITADTKPQQEARLWKLIQETGTE
LVVLARYMQILSDDLSGKLEGRCINIHHSFLPGFKGARPYHQAHARGVKVIGATAHYVTADLDEGPIIEQDVERISHRDH
PRDLVRKGRDIERRVLARAVRWHLEDRVLLNGRKTVVFTD
>Mature_280_residues
MILTLSCPDQPGIVARVSALLFERGANILDAQQFDDEETGRFFMRVVFDPGQADVAAWRDAFAPLGEQYGMTWQMRARGE
RRRVMILASQQDHCLADLIWRWRQGELPMDITAVVSNHPASTYPHTDLHGIAFHHLPITADTKPQQEARLWKLIQETGTE
LVVLARYMQILSDDLSGKLEGRCINIHHSFLPGFKGARPYHQAHARGVKVIGATAHYVTADLDEGPIIEQDVERISHRDH
PRDLVRKGRDIERRVLARAVRWHLEDRVLLNGRKTVVFTD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=109, Percent_Identity=34.8623853211009, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI209869995, Length=109, Percent_Identity=34.8623853211009, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI209869993, Length=109, Percent_Identity=34.8623853211009, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=39.3501805054152, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788846, Length=188, Percent_Identity=28.1914893617021, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=152, Percent_Identity=38.1578947368421, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31931; Mature: 31931

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILTLSCPDQPGIVARVSALLFERGANILDAQQFDDEETGRFFMRVVFDPGQADVAAWRD
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEEECCCCHHHHHHHH
AFAPLGEQYGMTWQMRARGERRRVMILASQQDHCLADLIWRWRQGELPMDITAVVSNHPA
HHHHCHHHHCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCCCC
STYPHTDLHGIAFHHLPITADTKPQQEARLWKLIQETGTELVVLARYMQILSDDLSGKLE
CCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
GRCINIHHSFLPGFKGARPYHQAHARGVKVIGATAHYVTADLDEGPIIEQDVERISHRDH
CEEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECEEEEEEEECCCCCCCHHHHHHHHCCCC
PRDLVRKGRDIERRVLARAVRWHLEDRVLLNGRKTVVFTD
CHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCEEEEEEC
>Mature Secondary Structure
MILTLSCPDQPGIVARVSALLFERGANILDAQQFDDEETGRFFMRVVFDPGQADVAAWRD
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEEECCCCHHHHHHHH
AFAPLGEQYGMTWQMRARGERRRVMILASQQDHCLADLIWRWRQGELPMDITAVVSNHPA
HHHHCHHHHCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCCCC
STYPHTDLHGIAFHHLPITADTKPQQEARLWKLIQETGTELVVLARYMQILSDDLSGKLE
CCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
GRCINIHHSFLPGFKGARPYHQAHARGVKVIGATAHYVTADLDEGPIIEQDVERISHRDH
CEEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECEEEEEEEECCCCCCCHHHHHHHHCCCC
PRDLVRKGRDIERRVLARAVRWHLEDRVLLNGRKTVVFTD
CHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]