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The map label for this gene is dusA [H]

Identifier: 197105805

GI number: 197105805

Start: 2655870

End: 2656901

Strand: Reverse

Name: dusA [H]

Synonym: PHZ_c2343

Alternate gene names: 197105805

Gene position: 2656901-2655870 (Counterclockwise)

Preceding gene: 197105807

Following gene: 197105801

Centisome position: 66.48

GC content: 71.41

Gene sequence:

>1032_bases
GTGCAGAAATATCAAGCTCATAGGTTCGCCATTGCACCTATGATGGACTGGACCGACCGGCACTGCCGGGTCCTGCACCG
GACGCTGTCGTCCCGGGCGCTGCTCTATACCGAGATGCTGACCACCGGGGCCGTGCTGCACGGCGACCGCGAGCGGCTGC
TCGCCTACGATCCCGTGGAACATCCGGTGGCGCTGCAGCTGGGCGGCTCCGAGCCGGCCGAGCTGGCGGCCGCCGCCCGG
ATCGGCGAGGCCGAGGGCTACGACGAGATCAACCTCAACGTGGGCTGCCCGTCCGACCGGGTGCAGTCGGGCCGGTTCGG
CGCCTGCCTGATGCGCGAGCCCGAGCTGGTGGCCGAGTGCATGGCGGCCATGGGCGCCGCGGTGAAGGTCCCCGTCACCG
TGAAGTGCCGGATCGGCGTGGACGACCAGGACCCCGAGGAGAGCCTGTTCACCCTGGTGGACCTCTGCGCGGAGGCGGGG
GTCACGAGCTTCGTCGTCCATGCGCGCAAGGCCTGGCTGAAGGGGCTCTCGCCGAAGGAGAACCGGGACGTCCCGCCGCT
GGACTATCCGCTGGTGTGGCGCCTGAAGGCCGAGCGCCCGGACCTGACCATCGTCATCAACGGCGGGATCGGCTCGCTGG
ATGAGGCTGAGGACCACCTGCGCCACGTGGACGGCGTCATGCTGGGCCGCGCCGCCTACCACACGCCCGATCTCCTGGGG
CAGGCCGACGCACGGATCTTCGGCGAGGACCGGCGGGTGAGCCCGTTCGAGGCGGTGGAGGCGTTCCGGCCCTATGTCGC
GGGCCAGCTCGCGAGGGGGACGCATCTGGCCGCCATGACCCGGCACATGCTGGGCCTGTTCCACGGCGTGCCGGGCGCGC
GCGCCTGGCGCCGGATCCTGACGGTGGACGGCGTGAAGGCGGGGGCGGGTCTGGAGGTGATCGACCGAGCTCTGGCGGCG
GTCTCGGAGCGGGCCTCAGGGCTGCAGGAACAGCGCCTCGCGGGTGGCCTCGAAACGGGCGAGGCGGCCTAG

Upstream 100 bases:

>100_bases
CCCTGTGCAGCGAGATCGCGAAAGTCCGCGAAAACCGGCGTGCAGGAGCGTAAAATGGCGCAAATGCATACCGATCCAAC
TAATTGAGTTTACGAGAAAA

Downstream 100 bases:

>100_bases
GTAGCTCATCCCGAGCAGCGAGGTGTCGAGACCCCGCTCGATGACGAGGGCGCCGACGTCGTCCAGACGCGCCCCGCCGA
CCGACACGGAGGCTAGCGTC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MQKYQAHRFAIAPMMDWTDRHCRVLHRTLSSRALLYTEMLTTGAVLHGDRERLLAYDPVEHPVALQLGGSEPAELAAAAR
IGEAEGYDEINLNVGCPSDRVQSGRFGACLMREPELVAECMAAMGAAVKVPVTVKCRIGVDDQDPEESLFTLVDLCAEAG
VTSFVVHARKAWLKGLSPKENRDVPPLDYPLVWRLKAERPDLTIVINGGIGSLDEAEDHLRHVDGVMLGRAAYHTPDLLG
QADARIFGEDRRVSPFEAVEAFRPYVAGQLARGTHLAAMTRHMLGLFHGVPGARAWRRILTVDGVKAGAGLEVIDRALAA
VSERASGLQEQRLAGGLETGEAA

Sequences:

>Translated_343_residues
MQKYQAHRFAIAPMMDWTDRHCRVLHRTLSSRALLYTEMLTTGAVLHGDRERLLAYDPVEHPVALQLGGSEPAELAAAAR
IGEAEGYDEINLNVGCPSDRVQSGRFGACLMREPELVAECMAAMGAAVKVPVTVKCRIGVDDQDPEESLFTLVDLCAEAG
VTSFVVHARKAWLKGLSPKENRDVPPLDYPLVWRLKAERPDLTIVINGGIGSLDEAEDHLRHVDGVMLGRAAYHTPDLLG
QADARIFGEDRRVSPFEAVEAFRPYVAGQLARGTHLAAMTRHMLGLFHGVPGARAWRRILTVDGVKAGAGLEVIDRALAA
VSERASGLQEQRLAGGLETGEAA
>Mature_343_residues
MQKYQAHRFAIAPMMDWTDRHCRVLHRTLSSRALLYTEMLTTGAVLHGDRERLLAYDPVEHPVALQLGGSEPAELAAAAR
IGEAEGYDEINLNVGCPSDRVQSGRFGACLMREPELVAECMAAMGAAVKVPVTVKCRIGVDDQDPEESLFTLVDLCAEAG
VTSFVVHARKAWLKGLSPKENRDVPPLDYPLVWRLKAERPDLTIVINGGIGSLDEAEDHLRHVDGVMLGRAAYHTPDLLG
QADARIFGEDRRVSPFEAVEAFRPYVAGQLARGTHLAAMTRHMLGLFHGVPGARAWRRILTVDGVKAGAGLEVIDRALAA
VSERASGLQEQRLAGGLETGEAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=241, Percent_Identity=29.8755186721992, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI40807366, Length=242, Percent_Identity=28.5123966942149, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI145693211, Length=319, Percent_Identity=57.6802507836991, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1789660, Length=241, Percent_Identity=28.6307053941909, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788462, Length=180, Percent_Identity=31.6666666666667, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17507177, Length=167, Percent_Identity=29.3413173652695, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=287, Percent_Identity=25.4355400696864, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24580595, Length=239, Percent_Identity=28.4518828451883, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19920448, Length=239, Percent_Identity=28.4518828451883, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37267; Mature: 37267

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKYQAHRFAIAPMMDWTDRHCRVLHRTLSSRALLYTEMLTTGAVLHGDRERLLAYDPVE
CCCCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCC
HPVALQLGGSEPAELAAAARIGEAEGYDEINLNVGCPSDRVQSGRFGACLMREPELVAEC
CCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCCCCEEECCCHHHHHHH
MAAMGAAVKVPVTVKCRIGVDDQDPEESLFTLVDLCAEAGVTSFVVHARKAWLKGLSPKE
HHHCCCEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
NRDVPPLDYPLVWRLKAERPDLTIVINGGIGSLDEAEDHLRHVDGVMLGRAAYHTPDLLG
CCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHCCCHHHC
QADARIFGEDRRVSPFEAVEAFRPYVAGQLARGTHLAAMTRHMLGLFHGVPGARAWRRIL
CCCHHEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
TVDGVKAGAGLEVIDRALAAVSERASGLQEQRLAGGLETGEAA
HHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQKYQAHRFAIAPMMDWTDRHCRVLHRTLSSRALLYTEMLTTGAVLHGDRERLLAYDPVE
CCCCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCC
HPVALQLGGSEPAELAAAARIGEAEGYDEINLNVGCPSDRVQSGRFGACLMREPELVAEC
CCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCCCCEEECCCHHHHHHH
MAAMGAAVKVPVTVKCRIGVDDQDPEESLFTLVDLCAEAGVTSFVVHARKAWLKGLSPKE
HHHCCCEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
NRDVPPLDYPLVWRLKAERPDLTIVINGGIGSLDEAEDHLRHVDGVMLGRAAYHTPDLLG
CCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHCCCHHHC
QADARIFGEDRRVSPFEAVEAFRPYVAGQLARGTHLAAMTRHMLGLFHGVPGARAWRRIL
CCCHHEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
TVDGVKAGAGLEVIDRALAAVSERASGLQEQRLAGGLETGEAA
HHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]