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The map label for this gene is amiD [H]

Identifier: 197105795

GI number: 197105795

Start: 2646804

End: 2647550

Strand: Reverse

Name: amiD [H]

Synonym: PHZ_c2333

Alternate gene names: 197105795

Gene position: 2647550-2646804 (Counterclockwise)

Preceding gene: 197105796

Following gene: 197105794

Centisome position: 66.25

GC content: 72.16

Gene sequence:

>747_bases
CTGGAACCGGTCCCAATGACGCCCCCCATGACGCTCATCGAGGCGCCGTCGCCCAACTTCAACGCCCGCCTCGCCCCGCC
GGACACGATCGTCCTGCACTACACGGGGATGGAGACGGGGCAGGCGGCCCTGGACCGTCTGCGCGATCCCGAGGCCAAGG
TCTCCTCCCACTACCTGGTGGAGGAGGACGGCCGGGTCTTCCGCCTGGTGCCCGAGGAGCGGCGCGCCTGGCACGCCGGG
GTCTCGTTCTGGAAGGGCCAGCGCAATCTCAACGACACCTCGATCGGCGTCGAGATCGTCAATCCGGGCCACGAGTTCGG
CTACCGGCCGTTTCCCGAGCCGCAGGTCGAGGCGGTGATCGCGCTGCTCGCCGACATCCGCAGCCGCTGGATGATCGAGG
ACGGCCGCATCGTGGGCCACTCGGACATCGCCCCCGACCGCAAGATCGACCCCGGCGAGCTGTTCCCGTGGAAGCGCCTC
GCGCAGGCCGGTCATGGCCTGTGGGCCGAGGCGGCGCCGGCCCCCGGCGCGCCGATCGGCGAGGGGGAGGAGGGCGCGGC
CGTCTTCGCCCTGCAGGCGGGGCTCACCCGCCTCGGATACGACCTGCCGCCTTCGGGCCGGTTCGACGCCGACACCACGA
CGGTGGTCTCGGCCTTCCAGCGCCATTGGCGTCCCGAGCGGATCGACGGGATCGCCGACGGCGAGACCCGGGCGCGGCTG
ATCGCGCTGCTGCGGCTCTCGGCTTGA

Upstream 100 bases:

>100_bases
CCTTCGGCCCGCGCATGGCCATCGGCACGGCGGTGGCGCTCGCCGGGGTGCTGATCATCGCCCTGCGCCGCAACCAGGTG
ATGCCGCTGCTGCTCGCCGT

Downstream 100 bases:

>100_bases
CCGCGCCCTTGCGCCGGCCCATCTCTCCTCGCGGATCAGACGGCCGGGCGGTCGCGGCCCTCCTTCGGGCGGGTCGAGGA
AAGTCCGGGCTCCACGACGG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MEPVPMTPPMTLIEAPSPNFNARLAPPDTIVLHYTGMETGQAALDRLRDPEAKVSSHYLVEEDGRVFRLVPEERRAWHAG
VSFWKGQRNLNDTSIGVEIVNPGHEFGYRPFPEPQVEAVIALLADIRSRWMIEDGRIVGHSDIAPDRKIDPGELFPWKRL
AQAGHGLWAEAAPAPGAPIGEGEEGAAVFALQAGLTRLGYDLPPSGRFDADTTTVVSAFQRHWRPERIDGIADGETRARL
IALLRLSA

Sequences:

>Translated_248_residues
MEPVPMTPPMTLIEAPSPNFNARLAPPDTIVLHYTGMETGQAALDRLRDPEAKVSSHYLVEEDGRVFRLVPEERRAWHAG
VSFWKGQRNLNDTSIGVEIVNPGHEFGYRPFPEPQVEAVIALLADIRSRWMIEDGRIVGHSDIAPDRKIDPGELFPWKRL
AQAGHGLWAEAAPAPGAPIGEGEEGAAVFALQAGLTRLGYDLPPSGRFDADTTTVVSAFQRHWRPERIDGIADGETRARL
IALLRLSA
>Mature_248_residues
MEPVPMTPPMTLIEAPSPNFNARLAPPDTIVLHYTGMETGQAALDRLRDPEAKVSSHYLVEEDGRVFRLVPEERRAWHAG
VSFWKGQRNLNDTSIGVEIVNPGHEFGYRPFPEPQVEAVIALLADIRSRWMIEDGRIVGHSDIAPDRKIDPGELFPWKRL
AQAGHGLWAEAAPAPGAPIGEGEEGAAVFALQAGLTRLGYDLPPSGRFDADTTTVVSAFQRHWRPERIDGIADGETRARL
IALLRLSA

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=236, Percent_Identity=41.1016949152542, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1786300, Length=165, Percent_Identity=33.9393939393939, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 27306; Mature: 27306

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPVPMTPPMTLIEAPSPNFNARLAPPDTIVLHYTGMETGQAALDRLRDPEAKVSSHYLV
CCCCCCCCCCEEEECCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHCCCCHHHHCCEEE
EEDGRVFRLVPEERRAWHAGVSFWKGQRNLNDTSIGVEIVNPGHEFGYRPFPEPQVEAVI
ECCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHCCCCCCCCHHHHHHH
ALLADIRSRWMIEDGRIVGHSDIAPDRKIDPGELFPWKRLAQAGHGLWAEAAPAPGAPIG
HHHHHHHHHEEEECCEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCC
EGEEGAAVFALQAGLTRLGYDLPPSGRFDADTTTVVSAFQRHWRPERIDGIADGETRARL
CCCCCCEEEEEHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCHHHHHH
IALLRLSA
HHHHHHCC
>Mature Secondary Structure
MEPVPMTPPMTLIEAPSPNFNARLAPPDTIVLHYTGMETGQAALDRLRDPEAKVSSHYLV
CCCCCCCCCCEEEECCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHCCCCHHHHCCEEE
EEDGRVFRLVPEERRAWHAGVSFWKGQRNLNDTSIGVEIVNPGHEFGYRPFPEPQVEAVI
ECCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHCCCCCCCCHHHHHHH
ALLADIRSRWMIEDGRIVGHSDIAPDRKIDPGELFPWKRLAQAGHGLWAEAAPAPGAPIG
HHHHHHHHHEEEECCEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCC
EGEEGAAVFALQAGLTRLGYDLPPSGRFDADTTTVVSAFQRHWRPERIDGIADGETRARL
CCCCCCEEEEEHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCHHHHHH
IALLRLSA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]