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The map label for this gene is mraW

Identifier: 197105793

GI number: 197105793

Start: 2644698

End: 2645630

Strand: Reverse

Name: mraW

Synonym: PHZ_c2331

Alternate gene names: 197105793

Gene position: 2645630-2644698 (Counterclockwise)

Preceding gene: 197105794

Following gene: 197105792

Centisome position: 66.2

GC content: 72.78

Gene sequence:

>933_bases
ATGACGACCCCGCACGTCCCCGTCCTGATGCAAGAGGTCGTGGACGCGCTCGCCCCTGCGCCCGGCCGGACGATCATCGA
CGGGACCTTCGGCGCCGGCGGCTACACCCGCGCGATCCTGGCGACCGGCGCCTCGGTGGTGGCCTTCGACCGCGACCCCA
CGGTCAGGCGCTTCGCCGAGGGGCTTCCCGCCGACCGCTTCCGCCTGGTGGAGGCGCGCTTTTCCGAGATGGACGAGGTG
CTGGGCGAGGGCGCCGCCGACGGCGTGGCCCTGGACCTCGGCGTCTCGTCCATGCAGCTCGACCAGGCCGAGCGCGGCTT
CTCGTTCATGCAGGACGGGCCGCTGGACATGCGCATGGGCGCGAGCGGGCCCACCGCCGCCGACCTCGTGAACACCGCCG
AGCCGGCCGAGCTCGCGCGGATCTTCTTCGTCTATGGAGAGGAGCGCGAGAGCCGCCGCATCGCCCGCGCCATCGCGCGC
CGGCGCGAGGAGCAGCCCTTCACCCGCACCCTGGAGCTGGCGGACTTCATCGAGAAGGCGCTGGGCGGCCGCCGCGGCGC
GAAGATCCACCCGGCGACCCGGGCCTTCCAGGGCCTTCGTATCGCCGTGAACGAGGAGCTATCCGAACTGGAGGCCGGCC
TTGCGGCCGCCGAGCGCGTCCTGAAGCCGGAGGGCCGCCTCTGCGTCGTCACGTTCCATTCGCTGGAAGACAGAATCGTG
AAGAGCTTCCTCTCGGTGCGAGCGGGTAGAACGCCGGCGGGATCGCGCCACGCGCCCCCGGTCCGCCAAGGCCCGGCGCC
CAGCTTCCAGCTCTTGTTCAACGGCGCCCGCGGGCCGTCCGAGACGGAGGTGGCGGCAAACCCCCGAGCCCGCTCGGCGA
AGCTGAGGGCGGCCGTGCGCACCGACGCGCCGGTCTGGAGGGATGCGGCATGA

Upstream 100 bases:

>100_bases
ACCGCGCCGCCCAGCGCGAGCTGGCCCGACAGGGGCTGGCCGAACTGCGCGCCCAGCAGCGGGCCGCACGCCTCGCCCAC
TCCGGACAGGGGAGCGCCGG

Downstream 100 bases:

>100_bases
ACCTGCTGAACCGCCGCATTCGCGGTTTCCGGCTGGTGGACCTGGTCGGCGTCGGCCTGCTGGTGGTGGTGATCCTGGGG
GTCTACCTGGCCAAGACCAT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTTPHVPVLMQEVVDALAPAPGRTIIDGTFGAGGYTRAILATGASVVAFDRDPTVRRFAEGLPADRFRLVEARFSEMDEV
LGEGAADGVALDLGVSSMQLDQAERGFSFMQDGPLDMRMGASGPTAADLVNTAEPAELARIFFVYGEERESRRIARAIAR
RREEQPFTRTLELADFIEKALGGRRGAKIHPATRAFQGLRIAVNEELSELEAGLAAAERVLKPEGRLCVVTFHSLEDRIV
KSFLSVRAGRTPAGSRHAPPVRQGPAPSFQLLFNGARGPSETEVAANPRARSAKLRAAVRTDAPVWRDAA

Sequences:

>Translated_310_residues
MTTPHVPVLMQEVVDALAPAPGRTIIDGTFGAGGYTRAILATGASVVAFDRDPTVRRFAEGLPADRFRLVEARFSEMDEV
LGEGAADGVALDLGVSSMQLDQAERGFSFMQDGPLDMRMGASGPTAADLVNTAEPAELARIFFVYGEERESRRIARAIAR
RREEQPFTRTLELADFIEKALGGRRGAKIHPATRAFQGLRIAVNEELSELEAGLAAAERVLKPEGRLCVVTFHSLEDRIV
KSFLSVRAGRTPAGSRHAPPVRQGPAPSFQLLFNGARGPSETEVAANPRARSAKLRAAVRTDAPVWRDAA
>Mature_309_residues
TTPHVPVLMQEVVDALAPAPGRTIIDGTFGAGGYTRAILATGASVVAFDRDPTVRRFAEGLPADRFRLVEARFSEMDEVL
GEGAADGVALDLGVSSMQLDQAERGFSFMQDGPLDMRMGASGPTAADLVNTAEPAELARIFFVYGEERESRRIARAIARR
REEQPFTRTLELADFIEKALGGRRGAKIHPATRAFQGLRIAVNEELSELEAGLAAAERVLKPEGRLCVVTFHSLEDRIVK
SFLSVRAGRTPAGSRHAPPVRQGPAPSFQLLFNGARGPSETEVAANPRARSAKLRAAVRTDAPVWRDAA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=340, Percent_Identity=42.0588235294118, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI165377202, Length=191, Percent_Identity=44.5026178010471, Blast_Score=151, Evalue=8e-37,
Organism=Escherichia coli, GI1786270, Length=312, Percent_Identity=47.4358974358974, Blast_Score=241, Evalue=5e-65,
Organism=Drosophila melanogaster, GI62472493, Length=336, Percent_Identity=40.1785714285714, Blast_Score=214, Evalue=5e-56,
Organism=Drosophila melanogaster, GI28571637, Length=336, Percent_Identity=40.1785714285714, Blast_Score=214, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_PHEZH (B4RFS8)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002131170.1
- ProteinModelPortal:   B4RFS8
- GeneID:   6791852
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c2331
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 33442; Mature: 33311

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: BINDING 50-50 BINDING 74-74 BINDING 92-92 BINDING 99-99

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPHVPVLMQEVVDALAPAPGRTIIDGTFGAGGYTRAILATGASVVAFDRDPTVRRFAE
CCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHC
GLPADRFRLVEARFSEMDEVLGEGAADGVALDLGVSSMQLDQAERGFSFMQDGPLDMRMG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCHHHCCCCCCEEEC
ASGPTAADLVNTAEPAELARIFFVYGEERESRRIARAIARRREEQPFTRTLELADFIEKA
CCCCCHHHHHCCCCHHHHHEEHEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LGGRRGAKIHPATRAFQGLRIAVNEELSELEAGLAAAERVLKPEGRLCVVTFHSLEDRIV
HCCCCCCCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHH
KSFLSVRAGRTPAGSRHAPPVRQGPAPSFQLLFNGARGPSETEVAANPRARSAKLRAAVR
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
TDAPVWRDAA
CCCCCCCCCC
>Mature Secondary Structure 
TTPHVPVLMQEVVDALAPAPGRTIIDGTFGAGGYTRAILATGASVVAFDRDPTVRRFAE
CCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHC
GLPADRFRLVEARFSEMDEVLGEGAADGVALDLGVSSMQLDQAERGFSFMQDGPLDMRMG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCHHHCCCCCCEEEC
ASGPTAADLVNTAEPAELARIFFVYGEERESRRIARAIARRREEQPFTRTLELADFIEKA
CCCCCHHHHHCCCCHHHHHEEHEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LGGRRGAKIHPATRAFQGLRIAVNEELSELEAGLAAAERVLKPEGRLCVVTFHSLEDRIV
HCCCCCCCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHH
KSFLSVRAGRTPAGSRHAPPVRQGPAPSFQLLFNGARGPSETEVAANPRARSAKLRAAVR
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
TDAPVWRDAA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA