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The map label for this gene is cysD [H]

Identifier: 197105725

GI number: 197105725

Start: 2565228

End: 2566181

Strand: Direct

Name: cysD [H]

Synonym: PHZ_c2263

Alternate gene names: 197105725

Gene position: 2565228-2566181 (Clockwise)

Preceding gene: 197105719

Following gene: 197105726

Centisome position: 64.19

GC content: 66.35

Gene sequence:

>954_bases
ATGGACGCCCGCGTCCTGACCCGCTCAACGATCTCCGAAGGCGCTGTGCCCCGCCAGACGCTCACCCACCTGCAGCGGCT
CGAGGCCGAGAGCATCCACATCATGCGCGAGGTCGTCGCCGAGTGCGAACGGCCGGTGATGCTCTACTCGATCGGCAAGG
ACTCGGCCGTGATGCTGCACTTGGCGGCTAAGGCCTTCTATCCTTCCAAACCGCCGTTCCCGCTGCTGCACGTCGACACG
ACCTGGAAGTTCCGGGCGATGTACGAGATGCGCGAACGCATGGCCAAGGAGCTCGGCTTCGAGCTGCTGGTCCACAAGAA
CCCGGACGCCGTCGCCCAGGGCGTCAATCCCTTCACCCACGGCAGCGCGGTCCACACGGACGTCTGGAAGACCGAGGGGC
TGAAGCAGGCGCTGGACAAGTACGGCTTCGACGCGGCCTTCGGCGGCGCCCGCCGCGACGAGGAGAAGTCCCGCGCCAAG
GAACGGATCTTCTCGTTCCGGTCGGCCCAGCATCGCTGGGATCCCAAGAACCAGCGGCCCGAGCTCTGGCGGCTCTACAA
CGCGCGCAAGAACCCCGGCGAGAGCATCCGGGTGTTCCCTCTGTCGAATTGGACCGAGCTCGACATCTGGCAATACATCT
ATCTCGAGCAGATCCCGATCGTGCCGCTCTACTTCGCCGCCGAGCGACCGGTGGTGGAGCGTGACGGGACGCTCATCATG
GTCGACGACGAGCGCTTCCCCCTGCGCGAGGGCGAGAGCCCGCAGATGCGCTCGGTCCGGTTCCGCACCCTCGGCTGCTA
CCCGCTGACCGGCGCCGTCGAGAGCACGGCGGCCACCCTGCCGCAGATCATCCAGGAGATGCTGCTCACCACCACGTCCG
AGCGCCAGGGCCGGGTCATCGACCACGACCAGTCCGCCTCGATGGAGAAGAAGAAGCAGGAGGGCTACTTCTGA

Upstream 100 bases:

>100_bases
CCTTGCGCCGCGCCCGGGCGGCGGCTAACTCGCGATCCAGGGCCGGCCCGGGCGCGCGCACGCGCACGGGAATATTCACT
CTTTGATGTAAGGTCCGCTC

Downstream 100 bases:

>100_bases
TGGCCCACCAGTCCGCCCTCATCGCCGAGGACATCGAGGCCTACCTGAAGCAGCACGAGGTGAAGAGCCTGCTGCGCTTC
ATCACCTGCGGCTCGGTGGA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MDARVLTRSTISEGAVPRQTLTHLQRLEAESIHIMREVVAECERPVMLYSIGKDSAVMLHLAAKAFYPSKPPFPLLHVDT
TWKFRAMYEMRERMAKELGFELLVHKNPDAVAQGVNPFTHGSAVHTDVWKTEGLKQALDKYGFDAAFGGARRDEEKSRAK
ERIFSFRSAQHRWDPKNQRPELWRLYNARKNPGESIRVFPLSNWTELDIWQYIYLEQIPIVPLYFAAERPVVERDGTLIM
VDDERFPLREGESPQMRSVRFRTLGCYPLTGAVESTAATLPQIIQEMLLTTTSERQGRVIDHDQSASMEKKKQEGYF

Sequences:

>Translated_317_residues
MDARVLTRSTISEGAVPRQTLTHLQRLEAESIHIMREVVAECERPVMLYSIGKDSAVMLHLAAKAFYPSKPPFPLLHVDT
TWKFRAMYEMRERMAKELGFELLVHKNPDAVAQGVNPFTHGSAVHTDVWKTEGLKQALDKYGFDAAFGGARRDEEKSRAK
ERIFSFRSAQHRWDPKNQRPELWRLYNARKNPGESIRVFPLSNWTELDIWQYIYLEQIPIVPLYFAAERPVVERDGTLIM
VDDERFPLREGESPQMRSVRFRTLGCYPLTGAVESTAATLPQIIQEMLLTTTSERQGRVIDHDQSASMEKKKQEGYF
>Mature_317_residues
MDARVLTRSTISEGAVPRQTLTHLQRLEAESIHIMREVVAECERPVMLYSIGKDSAVMLHLAAKAFYPSKPPFPLLHVDT
TWKFRAMYEMRERMAKELGFELLVHKNPDAVAQGVNPFTHGSAVHTDVWKTEGLKQALDKYGFDAAFGGARRDEEKSRAK
ERIFSFRSAQHRWDPKNQRPELWRLYNARKNPGESIRVFPLSNWTELDIWQYIYLEQIPIVPLYFAAERPVVERDGTLIM
VDDERFPLREGESPQMRSVRFRTLGCYPLTGAVESTAATLPQIIQEMLLTTTSERQGRVIDHDQSASMEKKKQEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=76.0942760942761, Blast_Score=474, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 36571; Mature: 36571

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARVLTRSTISEGAVPRQTLTHLQRLEAESIHIMREVVAECERPVMLYSIGKDSAVMLH
CCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEE
LAAKAFYPSKPPFPLLHVDTTWKFRAMYEMRERMAKELGFELLVHKNPDAVAQGVNPFTH
EHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCCCCCC
GSAVHTDVWKTEGLKQALDKYGFDAAFGGARRDEEKSRAKERIFSFRSAQHRWDPKNQRP
CCCEEHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
ELWRLYNARKNPGESIRVFPLSNWTELDIWQYIYLEQIPIVPLYFAAERPVVERDGTLIM
HHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEECCCEEEE
VDDERFPLREGESPQMRSVRFRTLGCYPLTGAVESTAATLPQIIQEMLLTTTSERQGRVI
ECCCCCCCCCCCCCCHHHEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEE
DHDQSASMEKKKQEGYF
CCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MDARVLTRSTISEGAVPRQTLTHLQRLEAESIHIMREVVAECERPVMLYSIGKDSAVMLH
CCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEE
LAAKAFYPSKPPFPLLHVDTTWKFRAMYEMRERMAKELGFELLVHKNPDAVAQGVNPFTH
EHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCCCCCC
GSAVHTDVWKTEGLKQALDKYGFDAAFGGARRDEEKSRAKERIFSFRSAQHRWDPKNQRP
CCCEEHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
ELWRLYNARKNPGESIRVFPLSNWTELDIWQYIYLEQIPIVPLYFAAERPVVERDGTLIM
HHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCEEECCCEEEE
VDDERFPLREGESPQMRSVRFRTLGCYPLTGAVESTAATLPQIIQEMLLTTTSERQGRVI
ECCCCCCCCCCCCCCHHHEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEE
DHDQSASMEKKKQEGYF
CCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]