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The map label for this gene is rfbB [C]

Identifier: 197105699

GI number: 197105699

Start: 2531857

End: 2532873

Strand: Direct

Name: rfbB [C]

Synonym: PHZ_c2237

Alternate gene names: 197105699

Gene position: 2531857-2532873 (Clockwise)

Preceding gene: 197105698

Following gene: 197105700

Centisome position: 63.36

GC content: 68.73

Gene sequence:

>1017_bases
TTGGCGATCCTGGTCACCGGCTCGGCCGGCTTCATCGGCTTCCACCTGAGCCGCCGGCTGCTGCAGCGGGGCGAACAGGT
CATCGGGGTCGACAACCTCAACGCCTACTACGATCCCAGCCTAAAGGCGGCGCGCCTGGCGCTGCTGGAGGCCGAGGCCG
GCTACCGCCACGCCCGCATCGACCTCGCCGACCGGGAGGCCATGGCCGCCCTGTTCGAGGAGACGCGCCCCGATGGGGTG
GTGAACCTGGCCGCCCAGGCCGGCGTGCGCTACAGCCTCGAGAACCCCGCCGCCTATGCGGACTCGAACGTGGTGGGCTT
TCTGAACGTCCTGGAAGGCTGCCGCGCCGTGCAGCCCAGACATCTCGTCTACGCCTCCACCAGCTCGGTCTACGGCGCCA
ACGGCAAGCTGCCGTTCAGCGTCCACGACCACGCCGTCCACCCGATCACGCTCTATGCGGCGACCAAGCTGGCCAACGAG
GCCATGGCCCACGCCTACGCCCACCTATTCGGCGTGCCGTGCACGGGCCTGCGCTTCTTCACCGTCTACGGTCCCTGGGG
CCGCCCCGACATGTCGCCGTTCAAGTTCCTCTCGGCGATCCTCGAGGGTCGGCCGATCGACGTCTACGGCCAGGGCCGGA
TGCAGCGGGACTTCACCTACGTCGACGACATCGTCGACGGCGTGATCGCCGCCCTGGACCGCCCGGCGCAGGCGAATCCT
GAGTGGGATCCGCAGCGGCCCGATCCCGCCTCCAGCGGCGTGGCTCCCTGGCGCATCTACAACATCGGGGCCAGCGAGCC
GGTCGAACTGATGCGCTACATCGAGACCTTCGAGCGCAAGCTGGGCTGCAAAGCGAAGCTCAACCTGATGCCGATGCAGC
CGGGCGACGTCGTCAGCACGGCCGCCGACGTGAGCGAGACCGTCCGGGACCTCGGCTATCGCCCGACCACCTCCATCGAG
GAGGGCGTCGGCCGCTTCGTGGACTGGTACCTCGACTACTACGGCAGCAAGGCCTAG

Upstream 100 bases:

>100_bases
CCCCTGCGCCGGGCCGGCTGACGCCGTCATCGACTTGGGCGCCTGCGGACGCTAGGGAATCCCCTGCGCGATCCTCCGCG
CGGAAACTTCGTGTGGCGCC

Downstream 100 bases:

>100_bases
CGGCGGGAGAACGGGCATCTTCGGCGCCGGCGCACTGAACCTGCTGGTCGCGCTTGGCCTCGGCCTGCTGATCGGCGCCG
AGCGCGAGCGCCGCAAGATC

Product: NAD-dependent epimerase/dehydratase family protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MAILVTGSAGFIGFHLSRRLLQRGEQVIGVDNLNAYYDPSLKAARLALLEAEAGYRHARIDLADREAMAALFEETRPDGV
VNLAAQAGVRYSLENPAAYADSNVVGFLNVLEGCRAVQPRHLVYASTSSVYGANGKLPFSVHDHAVHPITLYAATKLANE
AMAHAYAHLFGVPCTGLRFFTVYGPWGRPDMSPFKFLSAILEGRPIDVYGQGRMQRDFTYVDDIVDGVIAALDRPAQANP
EWDPQRPDPASSGVAPWRIYNIGASEPVELMRYIETFERKLGCKAKLNLMPMQPGDVVSTAADVSETVRDLGYRPTTSIE
EGVGRFVDWYLDYYGSKA

Sequences:

>Translated_338_residues
MAILVTGSAGFIGFHLSRRLLQRGEQVIGVDNLNAYYDPSLKAARLALLEAEAGYRHARIDLADREAMAALFEETRPDGV
VNLAAQAGVRYSLENPAAYADSNVVGFLNVLEGCRAVQPRHLVYASTSSVYGANGKLPFSVHDHAVHPITLYAATKLANE
AMAHAYAHLFGVPCTGLRFFTVYGPWGRPDMSPFKFLSAILEGRPIDVYGQGRMQRDFTYVDDIVDGVIAALDRPAQANP
EWDPQRPDPASSGVAPWRIYNIGASEPVELMRYIETFERKLGCKAKLNLMPMQPGDVVSTAADVSETVRDLGYRPTTSIE
EGVGRFVDWYLDYYGSKA
>Mature_337_residues
AILVTGSAGFIGFHLSRRLLQRGEQVIGVDNLNAYYDPSLKAARLALLEAEAGYRHARIDLADREAMAALFEETRPDGVV
NLAAQAGVRYSLENPAAYADSNVVGFLNVLEGCRAVQPRHLVYASTSSVYGANGKLPFSVHDHAVHPITLYAATKLANEA
MAHAYAHLFGVPCTGLRFFTVYGPWGRPDMSPFKFLSAILEGRPIDVYGQGRMQRDFTYVDDIVDGVIAALDRPAQANPE
WDPQRPDPASSGVAPWRIYNIGASEPVELMRYIETFERKLGCKAKLNLMPMQPGDVVSTAADVSETVRDLGYRPTTSIEE
GVGRFVDWYLDYYGSKA

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=336, Percent_Identity=27.6785714285714, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=25.8160237388724, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI56237023, Length=348, Percent_Identity=24.1379310344828, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI56118217, Length=348, Percent_Identity=24.1379310344828, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI189083684, Length=348, Percent_Identity=24.1379310344828, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=28.328611898017, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788353, Length=361, Percent_Identity=26.5927977839335, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1786974, Length=341, Percent_Identity=25.8064516129032, Blast_Score=104, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71982035, Length=357, Percent_Identity=27.7310924369748, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71982038, Length=360, Percent_Identity=27.2222222222222, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=26.3313609467456, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115532424, Length=340, Percent_Identity=23.2352941176471, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=356, Percent_Identity=24.1573033707865, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=354, Percent_Identity=24.0112994350282, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356223, Length=336, Percent_Identity=27.6785714285714, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19923002, Length=341, Percent_Identity=26.6862170087977, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37138; Mature: 37007

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILVTGSAGFIGFHLSRRLLQRGEQVIGVDNLNAYYDPSLKAARLALLEAEAGYRHARI
CEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCEECCCHHHHHHHHHHHCCCCCEEEE
DLADREAMAALFEETRPDGVVNLAAQAGVRYSLENPAAYADSNVVGFLNVLEGCRAVQPR
EHHHHHHHHHHHHHCCCCCEEEHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCC
HLVYASTSSVYGANGKLPFSVHDHAVHPITLYAATKLANEAMAHAYAHLFGVPCTGLRFF
EEEEEECCCEECCCCCCCEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TVYGPWGRPDMSPFKFLSAILEGRPIDVYGQGRMQRDFTYVDDIVDGVIAALDRPAQANP
EEECCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
EWDPQRPDPASSGVAPWRIYNIGASEPVELMRYIETFERKLGCKAKLNLMPMQPGDVVST
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
AADVSETVRDLGYRPTTSIEEGVGRFVDWYLDYYGSKA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AILVTGSAGFIGFHLSRRLLQRGEQVIGVDNLNAYYDPSLKAARLALLEAEAGYRHARI
EEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCEECCCHHHHHHHHHHHCCCCCEEEE
DLADREAMAALFEETRPDGVVNLAAQAGVRYSLENPAAYADSNVVGFLNVLEGCRAVQPR
EHHHHHHHHHHHHHCCCCCEEEHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCC
HLVYASTSSVYGANGKLPFSVHDHAVHPITLYAATKLANEAMAHAYAHLFGVPCTGLRFF
EEEEEECCCEECCCCCCCEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TVYGPWGRPDMSPFKFLSAILEGRPIDVYGQGRMQRDFTYVDDIVDGVIAALDRPAQANP
EEECCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
EWDPQRPDPASSGVAPWRIYNIGASEPVELMRYIETFERKLGCKAKLNLMPMQPGDVVST
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
AADVSETVRDLGYRPTTSIEEGVGRFVDWYLDYYGSKA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]