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The map label for this gene is rne [H]

Identifier: 197105656

GI number: 197105656

Start: 2480042

End: 2481157

Strand: Reverse

Name: rne [H]

Synonym: PHZ_c2193

Alternate gene names: 197105656

Gene position: 2481157-2480042 (Counterclockwise)

Preceding gene: 197105657

Following gene: 197105655

Centisome position: 62.09

GC content: 73.84

Gene sequence:

>1116_bases
ATCCTATTCGGGGGTGGTCGGCCTGCCGCTCTACGAGACCGCGAACCTTCTGACCGGCCTCGGATACAAGCTGCCATGAG
CACGCGTGTCGCCTATCTCGATCGCGGCGTCGGGGAGACCCGGGGCGTGGTGACGCTGGACGGGCGTCCCGAACGCCTGG
TGATCCGGCGTGACGGCGACGACGGCCGCCTGCTGCTGGGCGCTCGCCTCGCCGCCCGGGTGGAGACCGTCGAGCCTGCG
CTCGCCACCGCCTTTCTCGACCTCGGCGCCGGCGCCTCGGCCATCCTGCCTTTCAAGCCAGACGCGCGGCCGGCGCGGGG
CGGGTTCGTGGAGATCGAGATCCGCAGCGAGCCGCGTCGCGGCAAGCTGGCCATCGCGCGCCTGATCGGACCGGCCGAGG
GGCCGGCGCGGCTGCTGCAGCCGGCGCCCGAGCCGGCGCAGTATCTTCGCGCGCATGCGCGCGACGCCGAACTCGTCGAG
GGACGTGAGGCGCGTGACATGGCCGACGAGGCCGAGGCCCAGGCGCTTGAGGTGCTGCATCCGCTGCCGGGCGGCGGCGC
GCTGGCCATCGAGCCGACCCGCGCCCTCACGGCGATCGACGTGGACCTGGGCGAGCGCAAGGGCGCCGACGCCAAACGGG
TGACGCGCCAGGCGAACCTCGCCGCCCTGGCCGAGGCCGCCCGGCTGCTGCGGCTGAAGGGGCTGGGCGGGATCGTGGTG
ATCGATCTGGTCGGGCGCGGCCACGACGGAAATGCGCTGATGACAGCTGCCCGTGCGGCCTTTGGACCGGACAATCCCGG
CGTCGCCATCGGTCCGGTCGGCCGGTTCGGCACGATGGAGATATCGCTTCCGCGCCGGCAACGGCCGACCGCCGAACTGC
TCTGCCGCGACGACGGCGCGCTCTCGGACCGGACGCTGGCCCAGCGGCTGATCCGCGAGCTGCAGCGGGAAGGGGAGGCG
GACCGCGGCGGACGCTTCACCGGGCTCTGCTCGCCGGACGTGGCCGCCGGCGCCCAGCCGCTGCTGGTCCGTCTGGCGGA
GGCGATGGGTGCGCGGTTCTCGATCGCCGCCGAAGCGGGCAGGCCTCGCGACAGGTTCGAGGTGCGGCGGGCATGA

Upstream 100 bases:

>100_bases
CCGAGATCGCCGCCTACATCCGGTCGGGCGAGGGGCTCGGCAAGGCGGGCGGCTACGCGATCCAGGGCCGGGCGGGCGCC
TACGTCATCGACCTGCAGGG

Downstream 100 bases:

>100_bases
GCAAGGCGGGATGCCCCATCTGCGGCAAGCCGACGGACCCGGCGTATCGCCCGTTCTGTTCGCGGCGGTGCGCCGACGTC
GATCTCCAGCGCTGGTTGGC

Product: hypothetical protein

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MLFGGGRPAALRDREPSDRPRIQAAMSTRVAYLDRGVGETRGVVTLDGRPERLVIRRDGDDGRLLLGARLAARVETVEPA
LATAFLDLGAGASAILPFKPDARPARGGFVEIEIRSEPRRGKLAIARLIGPAEGPARLLQPAPEPAQYLRAHARDAELVE
GREARDMADEAEAQALEVLHPLPGGGALAIEPTRALTAIDVDLGERKGADAKRVTRQANLAALAEAARLLRLKGLGGIVV
IDLVGRGHDGNALMTAARAAFGPDNPGVAIGPVGRFGTMEISLPRRQRPTAELLCRDDGALSDRTLAQRLIRELQREGEA
DRGGRFTGLCSPDVAAGAQPLLVRLAEAMGARFSIAAEAGRPRDRFEVRRA

Sequences:

>Translated_371_residues
MLFGGGRPAALRDREPSDRPRIQAAMSTRVAYLDRGVGETRGVVTLDGRPERLVIRRDGDDGRLLLGARLAARVETVEPA
LATAFLDLGAGASAILPFKPDARPARGGFVEIEIRSEPRRGKLAIARLIGPAEGPARLLQPAPEPAQYLRAHARDAELVE
GREARDMADEAEAQALEVLHPLPGGGALAIEPTRALTAIDVDLGERKGADAKRVTRQANLAALAEAARLLRLKGLGGIVV
IDLVGRGHDGNALMTAARAAFGPDNPGVAIGPVGRFGTMEISLPRRQRPTAELLCRDDGALSDRTLAQRLIRELQREGEA
DRGGRFTGLCSPDVAAGAQPLLVRLAEAMGARFSIAAEAGRPRDRFEVRRA
>Mature_371_residues
MLFGGGRPAALRDREPSDRPRIQAAMSTRVAYLDRGVGETRGVVTLDGRPERLVIRRDGDDGRLLLGARLAARVETVEPA
LATAFLDLGAGASAILPFKPDARPARGGFVEIEIRSEPRRGKLAIARLIGPAEGPARLLQPAPEPAQYLRAHARDAELVE
GREARDMADEAEAQALEVLHPLPGGGALAIEPTRALTAIDVDLGERKGADAKRVTRQANLAALAEAARLLRLKGLGGIVV
IDLVGRGHDGNALMTAARAAFGPDNPGVAIGPVGRFGTMEISLPRRQRPTAELLCRDDGALSDRTLAQRLIRELQREGEA
DRGGRFTGLCSPDVAAGAQPLLVRLAEAMGARFSIAAEAGRPRDRFEVRRA

Specific function: This protein matures 5S rRNA from its precursors from all the rRNA genes. It is the major endoribonuclease participating in mRNA turnover [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=150, Percent_Identity=35.3333333333333, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 39482; Mature: 39482

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFGGGRPAALRDREPSDRPRIQAAMSTRVAYLDRGVGETRGVVTLDGRPERLVIRRDGD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCC
DGRLLLGARLAARVETVEPALATAFLDLGAGASAILPFKPDARPARGGFVEIEIRSEPRR
CCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCC
GKLAIARLIGPAEGPARLLQPAPEPAQYLRAHARDAELVEGREARDMADEAEAQALEVLH
CCEEEEEHHCCCCCHHHHHCCCCCHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHC
PLPGGGALAIEPTRALTAIDVDLGERKGADAKRVTRQANLAALAEAARLLRLKGLGGIVV
CCCCCCEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IDLVGRGHDGNALMTAARAAFGPDNPGVAIGPVGRFGTMEISLPRRQRPTAELLCRDDGA
EEEECCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHEEECCCCC
LSDRTLAQRLIRELQREGEADRGGRFTGLCSPDVAAGAQPLLVRLAEAMGARFSIAAEAG
CCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHCCEEEEEECCC
RPRDRFEVRRA
CCHHHHHCCCC
>Mature Secondary Structure
MLFGGGRPAALRDREPSDRPRIQAAMSTRVAYLDRGVGETRGVVTLDGRPERLVIRRDGD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCC
DGRLLLGARLAARVETVEPALATAFLDLGAGASAILPFKPDARPARGGFVEIEIRSEPRR
CCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCC
GKLAIARLIGPAEGPARLLQPAPEPAQYLRAHARDAELVEGREARDMADEAEAQALEVLH
CCEEEEEHHCCCCCHHHHHCCCCCHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHC
PLPGGGALAIEPTRALTAIDVDLGERKGADAKRVTRQANLAALAEAARLLRLKGLGGIVV
CCCCCCEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IDLVGRGHDGNALMTAARAAFGPDNPGVAIGPVGRFGTMEISLPRRQRPTAELLCRDDGA
EEEECCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHEEECCCCC
LSDRTLAQRLIRELQREGEADRGGRFTGLCSPDVAAGAQPLLVRLAEAMGARFSIAAEAG
CCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHCCEEEEEECCC
RPRDRFEVRRA
CCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]