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The map label for this gene is phaD [H]

Identifier: 197105608

GI number: 197105608

Start: 2416639

End: 2418219

Strand: Direct

Name: phaD [H]

Synonym: PHZ_c2145

Alternate gene names: 197105608

Gene position: 2416639-2418219 (Clockwise)

Preceding gene: 197105607

Following gene: 197105609

Centisome position: 60.47

GC content: 72.68

Gene sequence:

>1581_bases
GTGGCCGAGCACCTCCCCATCCTGCCGATCCTGCTGCCGCTGGCCTTCGGCGCGCTCCTGCTGCTGGCCGGCGGCCGCCG
GCGGCGCCTGGAGGCCGCCCTCGCGCTGGGCGGGTCCCTGGCCACCCTTTTCGCGGCGCTCGCCCTGGCGGTCGCGGTGG
ACGGCGCCGAGGGCTCGCTCTCGCTCACCTACCGACTGGGCGACTGGCCGGCGGCGGTGGCGATCGTGCTCGTGGCCGAC
CGGCTGGCCGCGCTCATGGTGCTGCTCGCCACCACCCTGGCGCTCGTCACGAGCGGCTTTGCCCTCGTCCGCTGGTCGCG
CCTTGGCCCCTACTACAGCGCGCTCACCCAGTTCCTGCTCATGGGGCTGAACGGCGCCTTCCTGACGGGCGACCTGTTCA
ACCTGTTCGTCTTCTTCGAGATCCTGCTCGCCGCCTCCTACGGCCTGCTGCTGCACGGATCAGGCGAGCGGCGGGTCGCG
GCGGGGCTGCACTACATCGTGCTGAACCTCGCCGCCTCGCTGCTGTTCCTGATCGGCGTGAGCGCGATCTTCGGCGTCAC
CGGCACCCTCAACATGGCGCTCCTCGCCGAGCGCATCCCCGAGGCTTCGGACGGGGCGCGGGGCCTGCTCCATGCGGGGG
CGGCGATCCTGACGGTGGCCTTCCTGGTCAAGGCGGCGGCCTGGCCGCTGGGGTTCTGGCTGCCCCGGGCCTACGACGCC
GCCGTTCCGCCCGCCGCCGCCATGTTCGCGATCATGACGAAGGTCGGGATCTACGCCGTGCTGCGCTTCGGGCTGCTGCT
GTTCGGCGACGAGGCGGGCGCCTCCGCGGGCTTCGGGCGCGACTGGATCTTCGCCATCGGCGGGGCGACGCTCGCGGTCG
GCGTGGTGGGGATGCTGGCGGTGCGCGACCTCGGCCGCATGGTCGGTTACAGCCTGCTCGTCTCGGTCGGCACGCTCCTG
GGCGTCGTCGCCTTCGCAGACGCGCAGGCGCTCGCCGGGGCGCTGCTCTATCTGGTGATCTCGACGCTGGGCGCGGCGGC
GTTCTTCCTGCTGAACGGGCTGATCGGGCCCGACGAATTCGAGGACGCGCCGACGCTGGAGGCCTACGACCCCGAGGACG
ATGGCCTGTCCGGGGATGAGGACGAGCGGCCGATCCTGATTTCCGCCCCGATCGGCGTCCTGAGCGGCGGGTTCCTCGTG
TGCACCCTCATCGTCGCCGGGATACCGCCCCTGCCCGGCTTCCTGGCCAAGGTCGCGATGCTTGCGCCGCTGCTGCAGGG
CCCCGCCGGACCGCTTCCAGGCGCCGCGCCGGTCATGGCGGCGATCATCATCGGCTCGAGCCTCTTCGCGCTGATCGCCC
TGGCCCGCGCCGGCATCCAGATCTGGTGGGCCGAGCCGGACCGGATCCCGCCGGCGATCCGCCTCGGCGAGGCGGCCCCG
CTGGGGCTGCTGCTGGTTGCCCTCCTTGCGCTCACGTTCGTCGTGGAGGCCCCGATGGGCTTCATGCAGCGCACCGCGGC
CCAACTCCTGGCTCCCGACCGGTACATGGGCGCCGTCCTCGACCGGGAGGGCGGGTCGTGA

Upstream 100 bases:

>100_bases
ATCGTGATCAGCTTCGCCACCACCGCCCTGCTGCTCGTCGTGGTCCTCGCGGCGCGCGGCCTGACCGGCGCCGACCACGT
CGACGGCAAGGACTGACGCG

Downstream 100 bases:

>100_bases
GGCGGCTGCTCCCCTATCCGACCATGAGCCTCTTCCTGATGGGGACCTGGCTGGTCCTCAACCAGAGCCTCGCGGCGGGC
CATCTGCTGATCGGCGCGCT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MAEHLPILPILLPLAFGALLLLAGGRRRRLEAALALGGSLATLFAALALAVAVDGAEGSLSLTYRLGDWPAAVAIVLVAD
RLAALMVLLATTLALVTSGFALVRWSRLGPYYSALTQFLLMGLNGAFLTGDLFNLFVFFEILLAASYGLLLHGSGERRVA
AGLHYIVLNLAASLLFLIGVSAIFGVTGTLNMALLAERIPEASDGARGLLHAGAAILTVAFLVKAAAWPLGFWLPRAYDA
AVPPAAAMFAIMTKVGIYAVLRFGLLLFGDEAGASAGFGRDWIFAIGGATLAVGVVGMLAVRDLGRMVGYSLLVSVGTLL
GVVAFADAQALAGALLYLVISTLGAAAFFLLNGLIGPDEFEDAPTLEAYDPEDDGLSGDEDERPILISAPIGVLSGGFLV
CTLIVAGIPPLPGFLAKVAMLAPLLQGPAGPLPGAAPVMAAIIIGSSLFALIALARAGIQIWWAEPDRIPPAIRLGEAAP
LGLLLVALLALTFVVEAPMGFMQRTAAQLLAPDRYMGAVLDREGGS

Sequences:

>Translated_526_residues
MAEHLPILPILLPLAFGALLLLAGGRRRRLEAALALGGSLATLFAALALAVAVDGAEGSLSLTYRLGDWPAAVAIVLVAD
RLAALMVLLATTLALVTSGFALVRWSRLGPYYSALTQFLLMGLNGAFLTGDLFNLFVFFEILLAASYGLLLHGSGERRVA
AGLHYIVLNLAASLLFLIGVSAIFGVTGTLNMALLAERIPEASDGARGLLHAGAAILTVAFLVKAAAWPLGFWLPRAYDA
AVPPAAAMFAIMTKVGIYAVLRFGLLLFGDEAGASAGFGRDWIFAIGGATLAVGVVGMLAVRDLGRMVGYSLLVSVGTLL
GVVAFADAQALAGALLYLVISTLGAAAFFLLNGLIGPDEFEDAPTLEAYDPEDDGLSGDEDERPILISAPIGVLSGGFLV
CTLIVAGIPPLPGFLAKVAMLAPLLQGPAGPLPGAAPVMAAIIIGSSLFALIALARAGIQIWWAEPDRIPPAIRLGEAAP
LGLLLVALLALTFVVEAPMGFMQRTAAQLLAPDRYMGAVLDREGGS
>Mature_525_residues
AEHLPILPILLPLAFGALLLLAGGRRRRLEAALALGGSLATLFAALALAVAVDGAEGSLSLTYRLGDWPAAVAIVLVADR
LAALMVLLATTLALVTSGFALVRWSRLGPYYSALTQFLLMGLNGAFLTGDLFNLFVFFEILLAASYGLLLHGSGERRVAA
GLHYIVLNLAASLLFLIGVSAIFGVTGTLNMALLAERIPEASDGARGLLHAGAAILTVAFLVKAAAWPLGFWLPRAYDAA
VPPAAAMFAIMTKVGIYAVLRFGLLLFGDEAGASAGFGRDWIFAIGGATLAVGVVGMLAVRDLGRMVGYSLLVSVGTLLG
VVAFADAQALAGALLYLVISTLGAAAFFLLNGLIGPDEFEDAPTLEAYDPEDDGLSGDEDERPILISAPIGVLSGGFLVC
TLIVAGIPPLPGFLAKVAMLAPLLQGPAGPLPGAAPVMAAIIIGSSLFALIALARAGIQIWWAEPDRIPPAIRLGEAAPL
GLLLVALLALTFVVEAPMGFMQRTAAQLLAPDRYMGAVLDREGGS

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=300, Percent_Identity=29.6666666666667, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788829, Length=435, Percent_Identity=25.7471264367816, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1788827, Length=305, Percent_Identity=26.5573770491803, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 54268; Mature: 54137

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHLPILPILLPLAFGALLLLAGGRRRRLEAALALGGSLATLFAALALAVAVDGAEGSL
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCE
SLTYRLGDWPAAVAIVLVADRLAALMVLLATTLALVTSGFALVRWSRLGPYYSALTQFLL
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHH
MGLNGAFLTGDLFNLFVFFEILLAASYGLLLHGSGERRVAAGLHYIVLNLAASLLFLIGV
HCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
SAIFGVTGTLNMALLAERIPEASDGARGLLHAGAAILTVAFLVKAAAWPLGFWLPRAYDA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCC
AVPPAAAMFAIMTKVGIYAVLRFGLLLFGDEAGASAGFGRDWIFAIGGATLAVGVVGMLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEECHHHHHHHHHHHHH
VRDLGRMVGYSLLVSVGTLLGVVAFADAQALAGALLYLVISTLGAAAFFLLNGLIGPDEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EDAPTLEAYDPEDDGLSGDEDERPILISAPIGVLSGGFLVCTLIVAGIPPLPGFLAKVAM
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LAPLLQGPAGPLPGAAPVMAAIIIGSSLFALIALARAGIQIWWAEPDRIPPAIRLGEAAP
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHCCCCCCC
LGLLLVALLALTFVVEAPMGFMQRTAAQLLAPDRYMGAVLDREGGS
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
AEHLPILPILLPLAFGALLLLAGGRRRRLEAALALGGSLATLFAALALAVAVDGAEGSL
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCE
SLTYRLGDWPAAVAIVLVADRLAALMVLLATTLALVTSGFALVRWSRLGPYYSALTQFLL
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHH
MGLNGAFLTGDLFNLFVFFEILLAASYGLLLHGSGERRVAAGLHYIVLNLAASLLFLIGV
HCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
SAIFGVTGTLNMALLAERIPEASDGARGLLHAGAAILTVAFLVKAAAWPLGFWLPRAYDA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCC
AVPPAAAMFAIMTKVGIYAVLRFGLLLFGDEAGASAGFGRDWIFAIGGATLAVGVVGMLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEECHHHHHHHHHHHHH
VRDLGRMVGYSLLVSVGTLLGVVAFADAQALAGALLYLVISTLGAAAFFLLNGLIGPDEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EDAPTLEAYDPEDDGLSGDEDERPILISAPIGVLSGGFLVCTLIVAGIPPLPGFLAKVAM
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LAPLLQGPAGPLPGAAPVMAAIIIGSSLFALIALARAGIQIWWAEPDRIPPAIRLGEAAP
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHCCCCCCC
LGLLLVALLALTFVVEAPMGFMQRTAAQLLAPDRYMGAVLDREGGS
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]