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The map label for this gene is gcvA [H]

Identifier: 197105508

GI number: 197105508

Start: 2288088

End: 2289044

Strand: Direct

Name: gcvA [H]

Synonym: PHZ_c2045

Alternate gene names: 197105508

Gene position: 2288088-2289044 (Clockwise)

Preceding gene: 197105501

Following gene: 197105509

Centisome position: 57.26

GC content: 73.25

Gene sequence:

>957_bases
ATGGAACGGAACAACCAGCGTCGCCGGCTGCCGCCGCTCAATGCGCTACGCGCCTTCGAGGCCGCCGCGCGCCACCTCAA
CTTCAGCCGGGCGGCCGACGAGCTGGCGGTGACGCCGGGGGCGGTCTCGCAGCAGATCCAGAATCTGGAGGACTACGTCG
GCGCGGCGCTCTTCAAGCGCACCCCCAAGGGCCTGCTGCTGACCGACGCGGCCCAGACCGCCCTGCCCGCCCTGCGCGAG
GCGTTCGACCGCCTGGCCGAGGCCGCCAGCCTGCTCACCGCCGCGGTGGACGGCCGGCGCCTGACCCTGACCGCCGCCCC
CTCCTTCGCCGCCAAGTGGCTGGCGCCGCGCCTGGGCGCCTTCGAGCGGGCCCACCCGCAGGTGGACGTGTGGCTGAACG
CGGGCATGGAGCTGGTGGACCTGACCGCGGGCGAGGTGGACATCGCCATCCGCTACGGCGCCGGCCGCTATCCGGGGCTG
GAGGTCACGCGGCTGATGAGCGAGACGGTGATCCCGGTGATCAGCCCCGACCTGCTGGCCCAGCAGCCGCTCGACACGCC
GGAGGACCTGGCCAACCACGTGCTGCTGCACGACGGCTCGCCCGACCTCGACGATTCCTGCCCCGACTGGTCGATGTGGC
TGGCCGCCCGGGGCCTGCGGGGCGTCGACGGCCAGCGTGGGCCGCGCTTCAACCAGTCGAGCCTGGTGATCGAGGCCGCC
GCCAACGGCCGGGGCGTGGCCCTGGCCAAGCGGGCGCTGGCCCAGGCCGACCTGGAGGCCGGCCGCCTGGTCGCGCCATT
GCAGATCGCCACCGCCGTCGACTTCGCCTATTACCTCGTCCATCCCAAAGCCAAGGGTCGGCTGCCGCAGGTGAAGGCCT
TCATCGGCTGGATCGAAGCGGAGGCCCGCGCGCACGAAGCGGCGCTCGCGGCCATCGACAACGGCGCCGGCATCTGA

Upstream 100 bases:

>100_bases
GCCGGCGAAAAGGGCCGATACACCAGCGGACGCTTGCAATAAGTGGCCGGGGCGCCCAATTCGCAACCCAAGGACTTCTG
CCGGGGCTGTCAGAGATTCT

Downstream 100 bases:

>100_bases
CGACCAGACGACGAGAGTCATGACGCTGATCACCGAAGACCGCCCCGCGCCCGCGGACTGCACCACCATGGCCGAAGTCC
GGAAGGGGGTGGACGCCCTC

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MERNNQRRRLPPLNALRAFEAAARHLNFSRAADELAVTPGAVSQQIQNLEDYVGAALFKRTPKGLLLTDAAQTALPALRE
AFDRLAEAASLLTAAVDGRRLTLTAAPSFAAKWLAPRLGAFERAHPQVDVWLNAGMELVDLTAGEVDIAIRYGAGRYPGL
EVTRLMSETVIPVISPDLLAQQPLDTPEDLANHVLLHDGSPDLDDSCPDWSMWLAARGLRGVDGQRGPRFNQSSLVIEAA
ANGRGVALAKRALAQADLEAGRLVAPLQIATAVDFAYYLVHPKAKGRLPQVKAFIGWIEAEARAHEAALAAIDNGAGI

Sequences:

>Translated_318_residues
MERNNQRRRLPPLNALRAFEAAARHLNFSRAADELAVTPGAVSQQIQNLEDYVGAALFKRTPKGLLLTDAAQTALPALRE
AFDRLAEAASLLTAAVDGRRLTLTAAPSFAAKWLAPRLGAFERAHPQVDVWLNAGMELVDLTAGEVDIAIRYGAGRYPGL
EVTRLMSETVIPVISPDLLAQQPLDTPEDLANHVLLHDGSPDLDDSCPDWSMWLAARGLRGVDGQRGPRFNQSSLVIEAA
ANGRGVALAKRALAQADLEAGRLVAPLQIATAVDFAYYLVHPKAKGRLPQVKAFIGWIEAEARAHEAALAAIDNGAGI
>Mature_318_residues
MERNNQRRRLPPLNALRAFEAAARHLNFSRAADELAVTPGAVSQQIQNLEDYVGAALFKRTPKGLLLTDAAQTALPALRE
AFDRLAEAASLLTAAVDGRRLTLTAAPSFAAKWLAPRLGAFERAHPQVDVWLNAGMELVDLTAGEVDIAIRYGAGRYPGL
EVTRLMSETVIPVISPDLLAQQPLDTPEDLANHVLLHDGSPDLDDSCPDWSMWLAARGLRGVDGQRGPRFNQSSLVIEAA
ANGRGVALAKRALAQADLEAGRLVAPLQIATAVDFAYYLVHPKAKGRLPQVKAFIGWIEAEARAHEAALAAIDNGAGI

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=300, Percent_Identity=46.3333333333333, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=34.9315068493151, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=32.8813559322034, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=27.7027027027027, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786401, Length=299, Percent_Identity=23.7458193979933, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787589, Length=275, Percent_Identity=23.2727272727273, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI157672245, Length=178, Percent_Identity=28.6516853932584, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081978, Length=273, Percent_Identity=27.8388278388278, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787128, Length=140, Percent_Identity=31.4285714285714, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787879, Length=291, Percent_Identity=27.1477663230241, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34148; Mature: 34148

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERNNQRRRLPPLNALRAFEAAARHLNFSRAADELAVTPGAVSQQIQNLEDYVGAALFKR
CCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
TPKGLLLTDAAQTALPALREAFDRLAEAASLLTAAVDGRRLTLTAAPSFAAKWLAPRLGA
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCC
FERAHPQVDVWLNAGMELVDLTAGEVDIAIRYGAGRYPGLEVTRLMSETVIPVISPDLLA
HHHCCCCEEEEECCCCEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCHHCCHHHHH
QQPLDTPEDLANHVLLHDGSPDLDDSCPDWSMWLAARGLRGVDGQRGPRFNQSSLVIEAA
CCCCCCHHHHHCEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEC
ANGRGVALAKRALAQADLEAGRLVAPLQIATAVDFAYYLVHPKAKGRLPQVKAFIGWIEA
CCCCCHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHH
EARAHEAALAAIDNGAGI
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MERNNQRRRLPPLNALRAFEAAARHLNFSRAADELAVTPGAVSQQIQNLEDYVGAALFKR
CCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
TPKGLLLTDAAQTALPALREAFDRLAEAASLLTAAVDGRRLTLTAAPSFAAKWLAPRLGA
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCC
FERAHPQVDVWLNAGMELVDLTAGEVDIAIRYGAGRYPGLEVTRLMSETVIPVISPDLLA
HHHCCCCEEEEECCCCEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCHHCCHHHHH
QQPLDTPEDLANHVLLHDGSPDLDDSCPDWSMWLAARGLRGVDGQRGPRFNQSSLVIEAA
CCCCCCHHHHHCEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEC
ANGRGVALAKRALAQADLEAGRLVAPLQIATAVDFAYYLVHPKAKGRLPQVKAFIGWIEA
CCCCCHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHH
EARAHEAALAAIDNGAGI
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]