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The map label for this gene is cysI [H]

Identifier: 197105505

GI number: 197105505

Start: 2285042

End: 2286709

Strand: Reverse

Name: cysI [H]

Synonym: PHZ_c2042

Alternate gene names: 197105505

Gene position: 2286709-2285042 (Counterclockwise)

Preceding gene: 197105506

Following gene: 197105504

Centisome position: 57.22

GC content: 69.42

Gene sequence:

>1668_bases
GTGAGGGCGGCCATGTACCGCTACGACGCCATCGACAAGGACTTCGTCGCCGACCGGACGGCGGAGTTCCGCGAACAGGT
CGCCCGCCGCCTGGCCGGCGAGATCACCGAGGAGCAGTTCAAGCCGATCCGGCTGATGAACGGCCTCTACCTGCAGCTCC
ACGCCTACATGCTGCGGATCGCGATCCCCTACGGCACGCTGACCTCGAAGAAGCTGCGCAAGCTGGCCTGGATCGCTTCG
ACCTACGACAAGGGCTACGGCCACTTCACGACCCGGACCAACCTCCAGCTCCACTGGATCAAGCTGAAGGACGCCCCCGA
GATCCTGGGGCATCTGGCCGACGTCGAGATGCACGCCATGCAGACCTCGGGCAACGTGGTCCGCAACGTGACCGCCGACC
CCTATGCCGGCGCGACGGCCGACGAGCTGGACGACCCGCGCGTCTGGGCCGAGGCGATCCGCCAGTGGTCGACTCTGCAC
CCCGAGTTCAGCTTCCTGCCGCGCAAGTTCAAGATCGCCGTGACCGCGGCCGCCAAGGACCGCACCGCGGCCAAGATCTA
CGACATCGGCCTGGCCATGCGCCGGGGCCGCGACGGCGAGCTGGGCTTCGAGGTGATGGTGGGCGGGGGCCTGGGCCGCA
CCCCGTACCTGGGCGCGACGATCCGCGAGTTCCTGCCGACCCGCCACCTTCTGTCGTACCTGGAGGCGATCCTGCGGGTC
TACAACCGCTACGGACGCCGCGACAACATCTGGAAGGCGCGCATCAAGGTGCTGGTCGCCTCGCTGGGCGCCGAGGAGTT
CGCCCGCCAGGTCGAGGCCGAATGGGCCAAGTCCGACCCCGGCCACGCCGACCTGCCCGACATCGAGCTCGCCCGCATCC
GCACCGACTTCCGGCCGGCGTTCGAGACGCTGCCCGCGCGCTCGGAGGCGCTGGAGACCGCCAAGGCCTCCAACCCCGCC
TTCGCCCGCTTCCTCCGGCACAACGTCCACCCGCACAAGCAGCCGGGCTACGCCATCGTCGACGTCTCGCTGAAGACGCC
GGGCCAGACGCCGGGCGACATCACCTCGGGCCAGATGGAGGTCGTCGCCGACCTCGCCGAGCGCTACTCGCTGGACGAGG
TGCGGGTGAACTACCAGCAGAACCTGCTGCTGCCGCACGTGAAGCTGGACGACCTGCCGACCGTCTGGGCGGCGCTGGAG
GCCGCCGGGCTCGCCACGCCGAACATCGACCTGATCAGCGACATCATCGCCTGCCCGGGCCTCGACTACTGCGCCCTGGC
CAACGCCCGGGCGATCCCGGTGGCCCAGCACATCGCAGAGCGCTTCGCCGATCCCGAGCGGGCCGAGACGATCGGCGAGC
TGAAGATCAAGATCAGCGGCTGCATCAACGCCTGCGGCCACCACCACGTCGGCCACATCGGCATCCTGGGCGTCGATAAG
AAGGGCGAGGAGTTCTACCAGGTCACCCTGGGCGGCTCGGGCGAGGACGACGCGGCCCTGGGCCAGATCCTCGGTCCGGC
GCTGCCCGCCGACAAGGTCACCGAGGCCGTCGAGCAGATGGTCGAGGTCTACCTGCGCGAGCGCCGCGACGGCGAGCGCT
TCCTCGACACCTTCCGCCGCACGGGCGTGGCGCCGTTCAAGGAGGCCGTCTATGCCGACGCACATTAG

Upstream 100 bases:

>100_bases
AGCACGGCAAGCAGGCCGCGGGCGGCGAGGACATCGCGGCCCTGCGGCGCGCCCATGGCGCGGCCCGTTCCACCGGCCGC
GTCGCCCTGATCAAGCGCAA

Downstream 100 bases:

>100_bases
GCTGCGCGACGGGCGCATGGCGCCTGTCGAGGACCCCTTCACCTACGTCGCCGACGACCAGGAACTCGTCCCCGGCGACG
TGATCCTGTCGCTGACCCGC

Product: sulfite reductase (NADPH) hemoprotein beta-component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MRAAMYRYDAIDKDFVADRTAEFREQVARRLAGEITEEQFKPIRLMNGLYLQLHAYMLRIAIPYGTLTSKKLRKLAWIAS
TYDKGYGHFTTRTNLQLHWIKLKDAPEILGHLADVEMHAMQTSGNVVRNVTADPYAGATADELDDPRVWAEAIRQWSTLH
PEFSFLPRKFKIAVTAAAKDRTAAKIYDIGLAMRRGRDGELGFEVMVGGGLGRTPYLGATIREFLPTRHLLSYLEAILRV
YNRYGRRDNIWKARIKVLVASLGAEEFARQVEAEWAKSDPGHADLPDIELARIRTDFRPAFETLPARSEALETAKASNPA
FARFLRHNVHPHKQPGYAIVDVSLKTPGQTPGDITSGQMEVVADLAERYSLDEVRVNYQQNLLLPHVKLDDLPTVWAALE
AAGLATPNIDLISDIIACPGLDYCALANARAIPVAQHIAERFADPERAETIGELKIKISGCINACGHHHVGHIGILGVDK
KGEEFYQVTLGGSGEDDAALGQILGPALPADKVTEAVEQMVEVYLRERRDGERFLDTFRRTGVAPFKEAVYADAH

Sequences:

>Translated_555_residues
MRAAMYRYDAIDKDFVADRTAEFREQVARRLAGEITEEQFKPIRLMNGLYLQLHAYMLRIAIPYGTLTSKKLRKLAWIAS
TYDKGYGHFTTRTNLQLHWIKLKDAPEILGHLADVEMHAMQTSGNVVRNVTADPYAGATADELDDPRVWAEAIRQWSTLH
PEFSFLPRKFKIAVTAAAKDRTAAKIYDIGLAMRRGRDGELGFEVMVGGGLGRTPYLGATIREFLPTRHLLSYLEAILRV
YNRYGRRDNIWKARIKVLVASLGAEEFARQVEAEWAKSDPGHADLPDIELARIRTDFRPAFETLPARSEALETAKASNPA
FARFLRHNVHPHKQPGYAIVDVSLKTPGQTPGDITSGQMEVVADLAERYSLDEVRVNYQQNLLLPHVKLDDLPTVWAALE
AAGLATPNIDLISDIIACPGLDYCALANARAIPVAQHIAERFADPERAETIGELKIKISGCINACGHHHVGHIGILGVDK
KGEEFYQVTLGGSGEDDAALGQILGPALPADKVTEAVEQMVEVYLRERRDGERFLDTFRRTGVAPFKEAVYADAH
>Mature_555_residues
MRAAMYRYDAIDKDFVADRTAEFREQVARRLAGEITEEQFKPIRLMNGLYLQLHAYMLRIAIPYGTLTSKKLRKLAWIAS
TYDKGYGHFTTRTNLQLHWIKLKDAPEILGHLADVEMHAMQTSGNVVRNVTADPYAGATADELDDPRVWAEAIRQWSTLH
PEFSFLPRKFKIAVTAAAKDRTAAKIYDIGLAMRRGRDGELGFEVMVGGGLGRTPYLGATIREFLPTRHLLSYLEAILRV
YNRYGRRDNIWKARIKVLVASLGAEEFARQVEAEWAKSDPGHADLPDIELARIRTDFRPAFETLPARSEALETAKASNPA
FARFLRHNVHPHKQPGYAIVDVSLKTPGQTPGDITSGQMEVVADLAERYSLDEVRVNYQQNLLLPHVKLDDLPTVWAALE
AAGLATPNIDLISDIIACPGLDYCALANARAIPVAQHIAERFADPERAETIGELKIKISGCINACGHHHVGHIGILGVDK
KGEEFYQVTLGGSGEDDAALGQILGPALPADKVTEAVEQMVEVYLRERRDGERFLDTFRRTGVAPFKEAVYADAH

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=289, Percent_Identity=25.6055363321799, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 61857; Mature: 61857

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAAMYRYDAIDKDFVADRTAEFREQVARRLAGEITEEQFKPIRLMNGLYLQLHAYMLRI
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AIPYGTLTSKKLRKLAWIASTYDKGYGHFTTRTNLQLHWIKLKDAPEILGHLADVEMHAM
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEEECCCHHHHHHHHHHHHHHH
QTSGNVVRNVTADPYAGATADELDDPRVWAEAIRQWSTLHPEFSFLPRKFKIAVTAAAKD
HCCCCEEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCHHHCCCEEEEEEEECCCC
RTAAKIYDIGLAMRRGRDGELGFEVMVGGGLGRTPYLGATIREFLPTRHLLSYLEAILRV
CCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHH
YNRYGRRDNIWKARIKVLVASLGAEEFARQVEAEWAKSDPGHADLPDIELARIRTDFRPA
HHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
FETLPARSEALETAKASNPAFARFLRHNVHPHKQPGYAIVDVSLKTPGQTPGDITSGQME
HHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHH
VVADLAERYSLDEVRVNYQQNLLLPHVKLDDLPTVWAALEAAGLATPNIDLISDIIACPG
HHHHHHHHCCHHHHHHCHHHCEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCC
LDYCALANARAIPVAQHIAERFADPERAETIGELKIKISGCINACGHHHVGHIGILGVDK
CCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHEEEEEHHHHHHCCCCCCCEEEEEECCC
KGEEFYQVTLGGSGEDDAALGQILGPALPADKVTEAVEQMVEVYLRERRDGERFLDTFRR
CCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TGVAPFKEAVYADAH
CCCCHHHHHHHCCCC
>Mature Secondary Structure
MRAAMYRYDAIDKDFVADRTAEFREQVARRLAGEITEEQFKPIRLMNGLYLQLHAYMLRI
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AIPYGTLTSKKLRKLAWIASTYDKGYGHFTTRTNLQLHWIKLKDAPEILGHLADVEMHAM
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEEECCCHHHHHHHHHHHHHHH
QTSGNVVRNVTADPYAGATADELDDPRVWAEAIRQWSTLHPEFSFLPRKFKIAVTAAAKD
HCCCCEEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCHHHCCCEEEEEEEECCCC
RTAAKIYDIGLAMRRGRDGELGFEVMVGGGLGRTPYLGATIREFLPTRHLLSYLEAILRV
CCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHH
YNRYGRRDNIWKARIKVLVASLGAEEFARQVEAEWAKSDPGHADLPDIELARIRTDFRPA
HHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
FETLPARSEALETAKASNPAFARFLRHNVHPHKQPGYAIVDVSLKTPGQTPGDITSGQME
HHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHH
VVADLAERYSLDEVRVNYQQNLLLPHVKLDDLPTVWAALEAAGLATPNIDLISDIIACPG
HHHHHHHHCCHHHHHHCHHHCEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCC
LDYCALANARAIPVAQHIAERFADPERAETIGELKIKISGCINACGHHHVGHIGILGVDK
CCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHEEEEEHHHHHHCCCCCCCEEEEEECCC
KGEEFYQVTLGGSGEDDAALGQILGPALPADKVTEAVEQMVEVYLRERRDGERFLDTFRR
CCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TGVAPFKEAVYADAH
CCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA