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The map label for this gene is folD

Identifier: 197105496

GI number: 197105496

Start: 2277177

End: 2278070

Strand: Reverse

Name: folD

Synonym: PHZ_c2033

Alternate gene names: 197105496

Gene position: 2278070-2277177 (Counterclockwise)

Preceding gene: 197105497

Following gene: 197105490

Centisome position: 57.01

GC content: 72.26

Gene sequence:

>894_bases
ATGACGGCGAAGATCATCGACGGCAAGGTCCTGGCCGAGCGCCTGCGCGGCCAGGTGGCGGACGAGGTGGCGCGGCTGCG
CGCCGACTACAGGCTCCAGCCGGGCCTGGCGGTGGTGCTGGTGGGCGAGGATCCCGCCTCCCAGGTCTATGTGCGGTCCA
AGGGCGAGCACTCCCTGGCCGTGGGCATGCACTCGGTCACCCACCGGCTGCCGGCCGACACCCGGCAGGACGAGCTGCTG
CGCCTGGTCGCCGAGCTCAACGCCGACCCGCTGATCCACGGCATCCTGGTCCAGCTTCCGCTGCCGAAGCACCTGGACGA
GAAGGCGGTGATCGCCGCCATCAGCCCCGACAAGGACGTCGACGGCCTGCATGTGGTCAACGCCGGGCGCCTGGCCAGCG
GCCTGCCGGCGCTGGTGCCCTGCACCCCGCTGGGCTGCCTCATCATGCTGCGCGAGACCCTCGGCGACCTGACGGGCAAG
CGCGCCGTCGTCGTCGGCCGCTCGCTGCTGGTGGGCAAGCCGGTGGCCCAGCTCCTGCTGCAGGCCGACTGCACCGTCAC
CATCGCCCACTCCCGCACCGTCGACCTGCCCGCCGTCTGCCGCGAGGCCGACATCCTGGTGGCCGCCGTGGGCCGTCCGC
GGATGATCCGCGGCGACTGGATCAAGCCGGGGGCGACGGTGATCGACGTGGGCATCAATCGCGTGCCGTTCGACGATCCC
GAGAAGGCCGCCCAGGGCAAGACCAAGCTGGTCGGCGACGTCCACTACAAGGAGGCGCTGCAGGTCGCCGGCGCGGTGAC
CCCCGTCCCCGGCGGCGTCGGCCTGATGACCGTCGCCTGCCTGCTGCAGAACACCGTCACGGCCGCCAAGCGCCTGGCCG
GCATCGAGGGCTAG

Upstream 100 bases:

>100_bases
CGCAGGAGGCGCTCACCGCCCTGCGCCACGAGGCCCACGCCTGGGCCGTCGCCGCATGGCTGAGATTGCGCGCGCGCGCC
TGAACCGCTAGCAGCGCGCC

Downstream 100 bases:

>100_bases
CCCGCCCTAGTCCTCCCGCCAGAGGCGGCCCTGCTGCGCCATCTCCGCGCCGTGGGCGAACATCCGCGACATCGCCTCGC
GGTCGAAGCGCAGCATGTTG

Product: FolD bifunctional protein

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTAKIIDGKVLAERLRGQVADEVARLRADYRLQPGLAVVLVGEDPASQVYVRSKGEHSLAVGMHSVTHRLPADTRQDELL
RLVAELNADPLIHGILVQLPLPKHLDEKAVIAAISPDKDVDGLHVVNAGRLASGLPALVPCTPLGCLIMLRETLGDLTGK
RAVVVGRSLLVGKPVAQLLLQADCTVTIAHSRTVDLPAVCREADILVAAVGRPRMIRGDWIKPGATVIDVGINRVPFDDP
EKAAQGKTKLVGDVHYKEALQVAGAVTPVPGGVGLMTVACLLQNTVTAAKRLAGIEG

Sequences:

>Translated_297_residues
MTAKIIDGKVLAERLRGQVADEVARLRADYRLQPGLAVVLVGEDPASQVYVRSKGEHSLAVGMHSVTHRLPADTRQDELL
RLVAELNADPLIHGILVQLPLPKHLDEKAVIAAISPDKDVDGLHVVNAGRLASGLPALVPCTPLGCLIMLRETLGDLTGK
RAVVVGRSLLVGKPVAQLLLQADCTVTIAHSRTVDLPAVCREADILVAAVGRPRMIRGDWIKPGATVIDVGINRVPFDDP
EKAAQGKTKLVGDVHYKEALQVAGAVTPVPGGVGLMTVACLLQNTVTAAKRLAGIEG
>Mature_296_residues
TAKIIDGKVLAERLRGQVADEVARLRADYRLQPGLAVVLVGEDPASQVYVRSKGEHSLAVGMHSVTHRLPADTRQDELLR
LVAELNADPLIHGILVQLPLPKHLDEKAVIAAISPDKDVDGLHVVNAGRLASGLPALVPCTPLGCLIMLRETLGDLTGKR
AVVVGRSLLVGKPVAQLLLQADCTVTIAHSRTVDLPAVCREADILVAAVGRPRMIRGDWIKPGATVIDVGINRVPFDDPE
KAAQGKTKLVGDVHYKEALQVAGAVTPVPGGVGLMTVACLLQNTVTAAKRLAGIEG

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=295, Percent_Identity=44.7457627118644, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI94721354, Length=304, Percent_Identity=45.7236842105263, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI222418558, Length=300, Percent_Identity=44.3333333333333, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI36796743, Length=203, Percent_Identity=30.5418719211823, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1786741, Length=289, Percent_Identity=50.5190311418685, Blast_Score=252, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17568735, Length=299, Percent_Identity=44.4816053511706, Blast_Score=218, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6319558, Length=290, Percent_Identity=45.8620689655172, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321643, Length=302, Percent_Identity=42.3841059602649, Blast_Score=234, Evalue=1e-62,
Organism=Drosophila melanogaster, GI17136816, Length=300, Percent_Identity=49.3333333333333, Blast_Score=257, Evalue=6e-69,
Organism=Drosophila melanogaster, GI17136818, Length=300, Percent_Identity=49.3333333333333, Blast_Score=256, Evalue=9e-69,
Organism=Drosophila melanogaster, GI24645718, Length=296, Percent_Identity=48.9864864864865, Blast_Score=254, Evalue=3e-68,
Organism=Drosophila melanogaster, GI17137370, Length=296, Percent_Identity=48.9864864864865, Blast_Score=254, Evalue=3e-68,
Organism=Drosophila melanogaster, GI62472483, Length=296, Percent_Identity=48.9864864864865, Blast_Score=254, Evalue=3e-68,
Organism=Drosophila melanogaster, GI45551871, Length=296, Percent_Identity=48.9864864864865, Blast_Score=254, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_PHEZH (B4RE11)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130873.1
- GeneID:   6791550
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c2033
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31371; Mature: 31240

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKIIDGKVLAERLRGQVADEVARLRADYRLQPGLAVVLVGEDPASQVYVRSKGEHSLA
CCCEECCHHHHHHHHHHHHHHHHHHHHHCCEECCCEEEEEECCCCCCEEEEECCCCCEEE
VGMHSVTHRLPADTRQDELLRLVAELNADPLIHGILVQLPLPKHLDEKAVIAAISPDKDV
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHEEEEEEECCCCCCCCCCEEEEEECCCCCC
DGLHVVNAGRLASGLPALVPCTPLGCLIMLRETLGDLTGKRAVVVGRSLLVGKPVAQLLL
CCEEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCHHHHHHH
QADCTVTIAHSRTVDLPAVCREADILVAAVGRPRMIRGDWIKPGATVIDVGINRVPFDDP
HCCCEEEEECCCCCCHHHHHCCCCEEEEECCCCCEEECCCCCCCCEEEECCCCCCCCCCH
EKAAQGKTKLVGDVHYKEALQVAGAVTPVPGGVGLMTVACLLQNTVTAAKRLAGIEG
HHHHCCCHHEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAKIIDGKVLAERLRGQVADEVARLRADYRLQPGLAVVLVGEDPASQVYVRSKGEHSLA
CCEECCHHHHHHHHHHHHHHHHHHHHHCCEECCCEEEEEECCCCCCEEEEECCCCCEEE
VGMHSVTHRLPADTRQDELLRLVAELNADPLIHGILVQLPLPKHLDEKAVIAAISPDKDV
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHEEEEEEECCCCCCCCCCEEEEEECCCCCC
DGLHVVNAGRLASGLPALVPCTPLGCLIMLRETLGDLTGKRAVVVGRSLLVGKPVAQLLL
CCEEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCHHHHHHH
QADCTVTIAHSRTVDLPAVCREADILVAAVGRPRMIRGDWIKPGATVIDVGINRVPFDDP
HCCCEEEEECCCCCCHHHHHCCCCEEEEECCCCCEEECCCCCCCCEEEECCCCCCCCCCH
EKAAQGKTKLVGDVHYKEALQVAGAVTPVPGGVGLMTVACLLQNTVTAAKRLAGIEG
HHHHCCCHHEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA