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The map label for this gene is ilvE [H]

Identifier: 197105480

GI number: 197105480

Start: 2258519

End: 2259616

Strand: Reverse

Name: ilvE [H]

Synonym: PHZ_c2017

Alternate gene names: 197105480

Gene position: 2259616-2258519 (Counterclockwise)

Preceding gene: 197105481

Following gene: 197105478

Centisome position: 56.54

GC content: 71.68

Gene sequence:

>1098_bases
ATGGAAGACTCGCTTCTCATCACCCGTCGCCCCCATGCCTCGCCGACCCCGCCGCACGCGCGGGCGGCCCTCCTGGAAGA
TCCCGGCTTCGGCAAGATCTTCACGGACCACATGGTGACGGTGCGCTGGGTAGAGGGGAAAGGCTGGCATGACGCCCAGG
TGCGCGCCCGCGAGCCGTTCCAGCTCGATCCCGCCGCGGCGGTGCTGCACTACGCCCAGGAGATCTTCGAGGGCCTGAAG
GCCTACCGCACCCGCGGCAACGACGTGGTGCTCTTCCGCCCGCGCGAGAACGCCCGCCGCTTCGTCGCCTCGGCCGACCG
CATGGCCATGCCGAAGCTGCCCGAGGACCTGTTCGTCGGCGCCATCGAGGCGCTGGTCCGCGTCGACGCCGACTGGATCC
CCACGGGGGAGGGCAGCCTCTATCTGCGGCCCTTCATGATCGCCACCGAGGCCTTCCTCGGCGTCCGGCCGGCGGCCGAA
TACACCTTCTGCGTCATCGCCTGTCCGGTGGGCGCCTACTTCAAGGGCGGGGCCAAGCCGGTCACCGTCTGGGTGTCCGA
CGCCTACACCCGCGCCGCGCCCGGCGGCACCGGGGCGGCCAAGTGCGGCGGCAACTACGCCGCCAGCCTGGTGGCCCAGG
CCGAGGCCATCCGCAACGGCTGCGACCAGGTCGTCTTCCTCGACGCGGCCGAGCACCGCTACGTCGAGGAGCTGGGCGGC
ATGAACGTCTTCTTCGTCTTCGACGACGGGACCATGGTCACCCCGCCGCTGGGCGGCACCATCCTGCCCGGCATCACCCG
CGACGCGATCCTGACCCTGGCCCGCGACGAGGGCCTGAACGTCCAGGAGCGGCTCTACTCGTTCGACGAGTGGCAGGCCG
ACGCGGCCAGCGGGCGGCTGAAGGAGGCCTTCGCCTGCGGCACCGCCGCGGTGCTGGCGGCCATCGGCGAGATCCGCCAC
GTGGGCGGCGCCTTCAAGATCGGCGGCGGCCAGGAGGGCCCGGTGACGACGCGGCTGCGCGACCAGCTCACCGGCATCCA
GCGCTGCACGGCGCCCGACCCGCACGGCTGGGTGCACAAGATCGACATGGAAGCCTGA

Upstream 100 bases:

>100_bases
TGTCTGGCCCTGGCGGAACACGCGCCTGCCAAGGATCGTATCCCCTGCAGGCGCTTGAGCATTGTGCTCCCGAGCGTTTT
CCAATTAGAAAATCGCCGTC

Downstream 100 bases:

>100_bases
GGGAAGCCTGAGCAGCCCCCGCCGCCCCTCAGCGGGCGGCGGCCTTTGCGGGAGCCGGGGCCTCGTCCCCGTGCAGGGTC
ATCAGCGATTCCATCAGGTC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MEDSLLITRRPHASPTPPHARAALLEDPGFGKIFTDHMVTVRWVEGKGWHDAQVRAREPFQLDPAAAVLHYAQEIFEGLK
AYRTRGNDVVLFRPRENARRFVASADRMAMPKLPEDLFVGAIEALVRVDADWIPTGEGSLYLRPFMIATEAFLGVRPAAE
YTFCVIACPVGAYFKGGAKPVTVWVSDAYTRAAPGGTGAAKCGGNYAASLVAQAEAIRNGCDQVVFLDAAEHRYVEELGG
MNVFFVFDDGTMVTPPLGGTILPGITRDAILTLARDEGLNVQERLYSFDEWQADAASGRLKEAFACGTAAVLAAIGEIRH
VGGAFKIGGGQEGPVTTRLRDQLTGIQRCTAPDPHGWVHKIDMEA

Sequences:

>Translated_365_residues
MEDSLLITRRPHASPTPPHARAALLEDPGFGKIFTDHMVTVRWVEGKGWHDAQVRAREPFQLDPAAAVLHYAQEIFEGLK
AYRTRGNDVVLFRPRENARRFVASADRMAMPKLPEDLFVGAIEALVRVDADWIPTGEGSLYLRPFMIATEAFLGVRPAAE
YTFCVIACPVGAYFKGGAKPVTVWVSDAYTRAAPGGTGAAKCGGNYAASLVAQAEAIRNGCDQVVFLDAAEHRYVEELGG
MNVFFVFDDGTMVTPPLGGTILPGITRDAILTLARDEGLNVQERLYSFDEWQADAASGRLKEAFACGTAAVLAAIGEIRH
VGGAFKIGGGQEGPVTTRLRDQLTGIQRCTAPDPHGWVHKIDMEA
>Mature_365_residues
MEDSLLITRRPHASPTPPHARAALLEDPGFGKIFTDHMVTVRWVEGKGWHDAQVRAREPFQLDPAAAVLHYAQEIFEGLK
AYRTRGNDVVLFRPRENARRFVASADRMAMPKLPEDLFVGAIEALVRVDADWIPTGEGSLYLRPFMIATEAFLGVRPAAE
YTFCVIACPVGAYFKGGAKPVTVWVSDAYTRAAPGGTGAAKCGGNYAASLVAQAEAIRNGCDQVVFLDAAEHRYVEELGG
MNVFFVFDDGTMVTPPLGGTILPGITRDAILTLARDEGLNVQERLYSFDEWQADAASGRLKEAFACGTAAVLAAIGEIRH
VGGAFKIGGGQEGPVTTRLRDQLTGIQRCTAPDPHGWVHKIDMEA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=326, Percent_Identity=40.4907975460123, Blast_Score=248, Evalue=9e-66,
Organism=Homo sapiens, GI38176287, Length=326, Percent_Identity=40.4907975460123, Blast_Score=247, Evalue=9e-66,
Organism=Homo sapiens, GI296010904, Length=326, Percent_Identity=40.4907975460123, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI50658084, Length=352, Percent_Identity=37.5, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI296010902, Length=326, Percent_Identity=35.5828220858896, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI296010900, Length=326, Percent_Identity=35.5828220858896, Blast_Score=201, Evalue=6e-52,
Organism=Homo sapiens, GI258614015, Length=282, Percent_Identity=37.5886524822695, Blast_Score=186, Evalue=4e-47,
Organism=Escherichia coli, GI48994963, Length=321, Percent_Identity=34.2679127725857, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17568601, Length=298, Percent_Identity=39.261744966443, Blast_Score=217, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI17565728, Length=333, Percent_Identity=33.9339339339339, Blast_Score=181, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6322608, Length=354, Percent_Identity=39.8305084745763, Blast_Score=243, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6322002, Length=326, Percent_Identity=42.3312883435583, Blast_Score=241, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24641779, Length=346, Percent_Identity=39.0173410404624, Blast_Score=221, Evalue=4e-58,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39555; Mature: 39555

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDSLLITRRPHASPTPPHARAALLEDPGFGKIFTDHMVTVRWVEGKGWHDAQVRAREPF
CCCCEEEEECCCCCCCCCCHHEEEECCCCCCCEEECCEEEEEEECCCCCCCCCCCCCCCC
QLDPAAAVLHYAQEIFEGLKAYRTRGNDVVLFRPRENARRFVASADRMAMPKLPEDLFVG
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCHHCCCCCCHHHHHH
AIEALVRVDADWIPTGEGSLYLRPFMIATEAFLGVRPAAEYTFCVIACPVGAYFKGGAKP
HHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCE
VTVWVSDAYTRAAPGGTGAAKCGGNYAASLVAQAEAIRNGCDQVVFLDAAEHRYVEELGG
EEEEEECCCHHCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCC
MNVFFVFDDGTMVTPPLGGTILPGITRDAILTLARDEGLNVQERLYSFDEWQADAASGRL
CEEEEEECCCCEECCCCCCCCCCCCCHHHEEEEECCCCCCHHHHHHCCHHHCCHHHCCHH
KEAFACGTAAVLAAIGEIRHVGGAFKIGGGQEGPVTTRLRDQLTGIQRCTAPDPHGWVHK
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEE
IDMEA
EECCC
>Mature Secondary Structure
MEDSLLITRRPHASPTPPHARAALLEDPGFGKIFTDHMVTVRWVEGKGWHDAQVRAREPF
CCCCEEEEECCCCCCCCCCHHEEEECCCCCCCEEECCEEEEEEECCCCCCCCCCCCCCCC
QLDPAAAVLHYAQEIFEGLKAYRTRGNDVVLFRPRENARRFVASADRMAMPKLPEDLFVG
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCHHCCCCCCHHHHHH
AIEALVRVDADWIPTGEGSLYLRPFMIATEAFLGVRPAAEYTFCVIACPVGAYFKGGAKP
HHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHCCCCCE
VTVWVSDAYTRAAPGGTGAAKCGGNYAASLVAQAEAIRNGCDQVVFLDAAEHRYVEELGG
EEEEEECCCHHCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCC
MNVFFVFDDGTMVTPPLGGTILPGITRDAILTLARDEGLNVQERLYSFDEWQADAASGRL
CEEEEEECCCCEECCCCCCCCCCCCCHHHEEEEECCCCCCHHHHHHCCHHHCCHHHCCHH
KEAFACGTAAVLAAIGEIRHVGGAFKIGGGQEGPVTTRLRDQLTGIQRCTAPDPHGWVHK
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEE
IDMEA
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]