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The map label for this gene is ycjG [H]

Identifier: 197105477

GI number: 197105477

Start: 2254509

End: 2255519

Strand: Reverse

Name: ycjG [H]

Synonym: PHZ_c2014

Alternate gene names: 197105477

Gene position: 2255519-2254509 (Counterclockwise)

Preceding gene: 197105478

Following gene: 197105476

Centisome position: 56.44

GC content: 73.49

Gene sequence:

>1011_bases
ATGACCGCCCTGCGCCAGCGCCTTGCCCTCAGCGTGGCCGAGGAGCCCGTCGCCCTCGCCGAGCCGTTCAGGATCACCGG
CTACGTCTTCACCGAGATGCCGGTCGTCGTCGTGCGCCTGCGGCAGGGCGCGGCGGAAGGGCAGGGGGAGGCCTCCGGCG
TCTACTACCTGGGGGACGACGTGCCCGCGATGCTCGCCGCCATCGAGGCGGCGCGGCCGCGGATCGAGGCCGGGATCGAC
CGCGAGGCGCTGCGCCGGGCGATGCCGCCGGGCGGCGGACGCAATGCGGTGGACGCCGCCCTCTGGGACCTCGAGGCCAA
GCTCACCGGCCGCCCCGCCTGGGCGCTCGCGGGCCTGGATCCGCCGAAGCCCCTGGTCACCACCTTCACCGTTGGCGCCG
CTGACCCGGATGTCATGGCGCGCAAGGCCGTCGCCTACGCCCAGGCCCGGGCGCTGAAGCTGAAACTCACCGGCGAGGCC
GAGCTCGACGCCGAGCGGGTGCGGGCGGTGCGCGCCGCCCGGCCCGACGTCTGGCTGGCCGTGGACGCCAACCAGGGCTT
CGTCCCCGACACGCTGGAGCGGCTGATGCCGGCGCTGGTCGACACCCGCGTCCAGCTCCTGGAGCAGCCGTTCGCCCGGG
GCCGTGAGAGCGACATGGAGGGGCTGGGCAGCCCCATCCCCGTGGCGGCCGACGAAAGCGTGCTGAGCTTCGCCGACCTC
GAGGAGATGGTCGGCCGCTTCGACGTCGTGAACATCAAGCTCGACAAATGCGGCGGGCTCACCGAGGGGCTGGCCATGGC
GCGGCAGGCCAGGCGTCTCGGCCTCGGGGTGATGGTCGGCAACATGGTGGGCACCAGCCTGGCCATGGGCCCGGCCTACC
TGCTCGGCCAGCTCTGCGAGGTGGTGGACCTCGACGGGCCGATCTTCATCACCCGCGACCGCCTGCCGTCGGTCAGCTAC
GCGGACGGCCTGATCGCCTATCCGGAGCACGGGTGGGGGAGCGCTCCGTGA

Upstream 100 bases:

>100_bases
CCACCTCGTGGGCCTGGGCGTGAACTACTGATCCACCGATGCGAGGCGTCCGGCCCCGGCCGGGCGCCTCCTTCCTTTTC
GCGCCATTCCCGGAGGTCGC

Downstream 100 bases:

>100_bases
CGAGTTCGGGCGCGCCGCCGGCCAGGGCGTGGTTCGGCCAGCCGCCGGGCCTGACGATCCTCTTCCTCACCGAGATGTGG
GAGAAGTTCTCGTTCTACGG

Product: mandelate racemase/muconate lactonizing enzyme

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTALRQRLALSVAEEPVALAEPFRITGYVFTEMPVVVVRLRQGAAEGQGEASGVYYLGDDVPAMLAAIEAARPRIEAGID
REALRRAMPPGGGRNAVDAALWDLEAKLTGRPAWALAGLDPPKPLVTTFTVGAADPDVMARKAVAYAQARALKLKLTGEA
ELDAERVRAVRAARPDVWLAVDANQGFVPDTLERLMPALVDTRVQLLEQPFARGRESDMEGLGSPIPVAADESVLSFADL
EEMVGRFDVVNIKLDKCGGLTEGLAMARQARRLGLGVMVGNMVGTSLAMGPAYLLGQLCEVVDLDGPIFITRDRLPSVSY
ADGLIAYPEHGWGSAP

Sequences:

>Translated_336_residues
MTALRQRLALSVAEEPVALAEPFRITGYVFTEMPVVVVRLRQGAAEGQGEASGVYYLGDDVPAMLAAIEAARPRIEAGID
REALRRAMPPGGGRNAVDAALWDLEAKLTGRPAWALAGLDPPKPLVTTFTVGAADPDVMARKAVAYAQARALKLKLTGEA
ELDAERVRAVRAARPDVWLAVDANQGFVPDTLERLMPALVDTRVQLLEQPFARGRESDMEGLGSPIPVAADESVLSFADL
EEMVGRFDVVNIKLDKCGGLTEGLAMARQARRLGLGVMVGNMVGTSLAMGPAYLLGQLCEVVDLDGPIFITRDRLPSVSY
ADGLIAYPEHGWGSAP
>Mature_335_residues
TALRQRLALSVAEEPVALAEPFRITGYVFTEMPVVVVRLRQGAAEGQGEASGVYYLGDDVPAMLAAIEAARPRIEAGIDR
EALRRAMPPGGGRNAVDAALWDLEAKLTGRPAWALAGLDPPKPLVTTFTVGAADPDVMARKAVAYAQARALKLKLTGEAE
LDAERVRAVRAARPDVWLAVDANQGFVPDTLERLMPALVDTRVQLLEQPFARGRESDMEGLGSPIPVAADESVLSFADLE
EMVGRFDVVNIKLDKCGGLTEGLAMARQARRLGLGVMVGNMVGTSLAMGPAYLLGQLCEVVDLDGPIFITRDRLPSVSYA
DGLIAYPEHGWGSAP

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=314, Percent_Identity=37.5796178343949, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 35856; Mature: 35725

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALRQRLALSVAEEPVALAEPFRITGYVFTEMPVVVVRLRQGAAEGQGEASGVYYLGDD
CHHHHHHHHHHHHCCCCHHCCCEEEEEEEEHHCHHHHHHHHHCCCCCCCCCCCEEEECCC
VPAMLAAIEAARPRIEAGIDREALRRAMPPGGGRNAVDAALWDLEAKLTGRPAWALAGLD
HHHHHHHHHHHCCHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCC
PPKPLVTTFTVGAADPDVMARKAVAYAQARALKLKLTGEAELDAERVRAVRAARPDVWLA
CCCCEEEEEEECCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHCCCCEEEE
VDANQGFVPDTLERLMPALVDTRVQLLEQPFARGRESDMEGLGSPIPVAADESVLSFADL
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHH
EEMVGRFDVVNIKLDKCGGLTEGLAMARQARRLGLGVMVGNMVGTSLAMGPAYLLGQLCE
HHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VVDLDGPIFITRDRLPSVSYADGLIAYPEHGWGSAP
HHCCCCCEEEECCCCCCCCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
TALRQRLALSVAEEPVALAEPFRITGYVFTEMPVVVVRLRQGAAEGQGEASGVYYLGDD
HHHHHHHHHHHHCCCCHHCCCEEEEEEEEHHCHHHHHHHHHCCCCCCCCCCCEEEECCC
VPAMLAAIEAARPRIEAGIDREALRRAMPPGGGRNAVDAALWDLEAKLTGRPAWALAGLD
HHHHHHHHHHHCCHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCC
PPKPLVTTFTVGAADPDVMARKAVAYAQARALKLKLTGEAELDAERVRAVRAARPDVWLA
CCCCEEEEEEECCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHCCCCEEEE
VDANQGFVPDTLERLMPALVDTRVQLLEQPFARGRESDMEGLGSPIPVAADESVLSFADL
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHH
EEMVGRFDVVNIKLDKCGGLTEGLAMARQARRLGLGVMVGNMVGTSLAMGPAYLLGQLCE
HHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VVDLDGPIFITRDRLPSVSYADGLIAYPEHGWGSAP
HHCCCCCEEEECCCCCCCCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]