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The map label for this gene is xthA [H]

Identifier: 197105436

GI number: 197105436

Start: 2203665

End: 2204444

Strand: Direct

Name: xthA [H]

Synonym: PHZ_c1973

Alternate gene names: 197105436

Gene position: 2203665-2204444 (Clockwise)

Preceding gene: 197105432

Following gene: 197105437

Centisome position: 55.14

GC content: 68.85

Gene sequence:

>780_bases
ATGCGCATCGCCACCTGGAACGTGAACTCGATCAACGCGCGCCTGGAGACCGTCGTGCGCTGGTTCGAGGCGGCGCAGCC
GGACGTGGCGTGCCTGCAGGAGATCAAGTGCGTCGACGAGAAGTTCCCGGCCGAACCCTTTGAACGGCTTGGCTATAATC
TCGCGATCCACGGCCAGAAGACCTACAACGGCGTGGCTCTCCTCTCGAAGCGGCCGCTGGAGGACGTGACCCGCGGCCTG
CCGGAGGGCGACGGCGACGAGCACGCCCGCTACATCGAGGCGGTGGTCTCGGGACCCCAGCCCGTTCGGGTGGCGTCGAT
CTACCTTCCGAACGGCAATCCGGTGGGGACCGAGAAGTTCGCCTACAAGCTGGCCTGGATGGGCCGGCTGCACCGCCATG
CCCAGAACCTGCTCAAGCTCGAGGAGCCGCTCGTCCTGGCCGGCGACTACAACGTCATCCCCGAGCCGCGCGACGCCGAG
TTCCCCAAGAACTGGGAGGACGACGCCCTCTTCCAGCCGCAGAGCCGGGCTGCCTTCCGGGCGCTGAAGAACCTCGGCCT
CACCGAGGCCTACCTGCAGGCCGACGGCGCGCCCGGCGCCTACACGTTCTGGGACTACCAGGCCGGGGCCTGGCAGCGGA
ACAACGGCATCCGCATCGACCACGCCCTGCTCTCGCCCCAGGCCGCCGACCTGCTGCGCGGCGTGACCATCCACCGCGAC
GTGCGCGGCTGGGAGAAGCCCTCGGACCACGTCCCGCTGGTGGTCGAACTGGACGCCTGA

Upstream 100 bases:

>100_bases
TAAGTATTTCCTTAAGTATCAGCCGATGGCGCAACGCGCAAGCCATTGAGCCCGGCCGCGCGCCCCACTAGCTTCCGCTC
CACCTGAGCCGGAGCCTTCG

Downstream 100 bases:

>100_bases
GCTGCGCCGTAGCCCGACTCGCCGAAACGGGCGTAGGCTGAGCGAATGGACGAGGTCGGGCGACTGATCGTGCTGATGGC
GATCGCCGGCGGCGCCTTCA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MRIATWNVNSINARLETVVRWFEAAQPDVACLQEIKCVDEKFPAEPFERLGYNLAIHGQKTYNGVALLSKRPLEDVTRGL
PEGDGDEHARYIEAVVSGPQPVRVASIYLPNGNPVGTEKFAYKLAWMGRLHRHAQNLLKLEEPLVLAGDYNVIPEPRDAE
FPKNWEDDALFQPQSRAAFRALKNLGLTEAYLQADGAPGAYTFWDYQAGAWQRNNGIRIDHALLSPQAADLLRGVTIHRD
VRGWEKPSDHVPLVVELDA

Sequences:

>Translated_259_residues
MRIATWNVNSINARLETVVRWFEAAQPDVACLQEIKCVDEKFPAEPFERLGYNLAIHGQKTYNGVALLSKRPLEDVTRGL
PEGDGDEHARYIEAVVSGPQPVRVASIYLPNGNPVGTEKFAYKLAWMGRLHRHAQNLLKLEEPLVLAGDYNVIPEPRDAE
FPKNWEDDALFQPQSRAAFRALKNLGLTEAYLQADGAPGAYTFWDYQAGAWQRNNGIRIDHALLSPQAADLLRGVTIHRD
VRGWEKPSDHVPLVVELDA
>Mature_259_residues
MRIATWNVNSINARLETVVRWFEAAQPDVACLQEIKCVDEKFPAEPFERLGYNLAIHGQKTYNGVALLSKRPLEDVTRGL
PEGDGDEHARYIEAVVSGPQPVRVASIYLPNGNPVGTEKFAYKLAWMGRLHRHAQNLLKLEEPLVLAGDYNVIPEPRDAE
FPKNWEDDALFQPQSRAAFRALKNLGLTEAYLQADGAPGAYTFWDYQAGAWQRNNGIRIDHALLSPQAADLLRGVTIHRD
VRGWEKPSDHVPLVVELDA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=264, Percent_Identity=31.8181818181818, Blast_Score=110, Evalue=8e-25,
Organism=Homo sapiens, GI18375503, Length=264, Percent_Identity=31.8181818181818, Blast_Score=110, Evalue=8e-25,
Organism=Homo sapiens, GI18375501, Length=264, Percent_Identity=31.8181818181818, Blast_Score=110, Evalue=8e-25,
Organism=Escherichia coli, GI1788046, Length=265, Percent_Identity=33.2075471698113, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71989536, Length=266, Percent_Identity=27.4436090225564, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221330655, Length=261, Percent_Identity=30.2681992337165, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17136678, Length=261, Percent_Identity=30.2681992337165, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29073; Mature: 29073

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATWNVNSINARLETVVRWFEAAQPDVACLQEIKCVDEKFPAEPFERLGYNLAIHGQK
CEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCC
TYNGVALLSKRPLEDVTRGLPEGDGDEHARYIEAVVSGPQPVRVASIYLPNGNPVGTEKF
CCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHH
AYKLAWMGRLHRHAQNLLKLEEPLVLAGDYNVIPEPRDAEFPKNWEDDALFQPQSRAAFR
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ALKNLGLTEAYLQADGAPGAYTFWDYQAGAWQRNNGIRIDHALLSPQAADLLRGVTIHRD
HHHHCCCHHHHHHCCCCCCCEEEEECCCCCEECCCCEEEEHHHCCCHHHHHHHCCHHHHH
VRGWEKPSDHVPLVVELDA
CCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MRIATWNVNSINARLETVVRWFEAAQPDVACLQEIKCVDEKFPAEPFERLGYNLAIHGQK
CEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCC
TYNGVALLSKRPLEDVTRGLPEGDGDEHARYIEAVVSGPQPVRVASIYLPNGNPVGTEKF
CCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHH
AYKLAWMGRLHRHAQNLLKLEEPLVLAGDYNVIPEPRDAEFPKNWEDDALFQPQSRAAFR
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ALKNLGLTEAYLQADGAPGAYTFWDYQAGAWQRNNGIRIDHALLSPQAADLLRGVTIHRD
HHHHCCCHHHHHHCCCCCCCEEEEECCCCCEECCCCEEEEHHHCCCHHHHHHHCCHHHHH
VRGWEKPSDHVPLVVELDA
CCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]