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The map label for this gene is amiC [H]

Identifier: 197105304

GI number: 197105304

Start: 2062536

End: 2063684

Strand: Reverse

Name: amiC [H]

Synonym: PHZ_c1841

Alternate gene names: 197105304

Gene position: 2063684-2062536 (Counterclockwise)

Preceding gene: 197105309

Following gene: 197105303

Centisome position: 51.64

GC content: 70.32

Gene sequence:

>1149_bases
ATGGCCGCCGGCCTGGGCCTCGCCGTGGCCGCGTCGCACGCCGCGCCCGACCCCGTCGGGCTGCTGAAGGTCCGCCTGGG
CGGCGACAGCGCCGAGACCCGGATCGTCCTCGACCTCGCCAAGTCGGCCAATGTGAAGATCGTCTCGGACGGCGCCGCCG
ATCGCCGGATCGTCATCCATATGCCGGGGGTGCAGGCCGCCGAGGAGCTGCAGGGCCCGGGCCGGGGCCTCGTCAAGGCC
TGGATGGTCGACGAGGTGCAGGGCGGGGCGCGCCTGCAGATGGACCTCGCCGCCGACGCCAAGGTGAAGCGCCGCTTCCT
GCTGCCGCCCGCCGACGGCGTCGAACACTACCGCTATGTCGTGGACGTGGCCGCGGCGCCGGGGGCGAGCCTGACAAAGG
CCTCGCAGGCCGGCCCCGTCGACGGCGCCCTGCGGACCAAGTTCCTGCCCGCCCGCAAGTCGGCCCTCCCGCTGAAGAAG
GTGGTCGTCGTCGACGCCGGCCACGGCGGCAAGGACGTCGGCGCGGTCGGCTCCAAGGGCTACGAGAAGGACATCAACCT
CGCCGCTGCCCAGGCGCTGAAGGACAAGCTGGAAAAGACCGGCCGCTACAAGGTGGTCATGACCCGCAACTCGGACGTCT
ACATTCCGCTTGAAACGCGGGTGCGGATCGCCCAGCGGGCCAACGCCGACCTGTTCATCTCGCTGCACTCCGATTCCGGA
CCCGACACCAGCCTGAAGGGCGCCAGCGTCTACACGCTGTCGGACAAGGCCTCGCGCCGCTCGACCAAGTTCGTCAGCAA
GGACGACTGGTTCATGAAGGCCAGCCTGACCGGCGATCAGGGGGTGCGCGACATCCTCTTCGACCTCACCCAACGGGCGA
CGCGCAACCGCTCGGCCGTCTTCGCCCAGACCCTCCTGTCCCATATCGAGGACGAGGCCCCGCTGCTGCGCCGCAGCCAC
CGGGACGCCGGTCTCGTGGTGCTGCTGGCTCCGGACGTCCCGGCGGTGCTTCTGGAGATGGGGTTCGTCAACAATCCCGA
GGACGAGGCCCGCCTGCGCGATCCCCGATCCCGCGCCCGCCTCATGGACTCGGTCGCCGACGCCATCGACGACCACTTCG
GCCACGACATGAAGGTTGCGGCGCGCTGA

Upstream 100 bases:

>100_bases
CGCATGTTGCGACTCCGCGTTGCGGAGCGAGGCTGGGCGTATGTTTGGGCGAGTGAGGGGGCTTCTGGCCGGGACTCGGG
GGCGCTTGGCGCTCGCGGCG

Downstream 100 bases:

>100_bases
CGCGGCGGCGTTGGCGCCCGGCCCGACACAGGGTAGACGACGCCTCATAAGGTGGACGCGGCGCCCCCGGAGGCTCGTCT
AAGTTGAACTCCCCCGCAGG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MAAGLGLAVAASHAAPDPVGLLKVRLGGDSAETRIVLDLAKSANVKIVSDGAADRRIVIHMPGVQAAEELQGPGRGLVKA
WMVDEVQGGARLQMDLAADAKVKRRFLLPPADGVEHYRYVVDVAAAPGASLTKASQAGPVDGALRTKFLPARKSALPLKK
VVVVDAGHGGKDVGAVGSKGYEKDINLAAAQALKDKLEKTGRYKVVMTRNSDVYIPLETRVRIAQRANADLFISLHSDSG
PDTSLKGASVYTLSDKASRRSTKFVSKDDWFMKASLTGDQGVRDILFDLTQRATRNRSAVFAQTLLSHIEDEAPLLRRSH
RDAGLVVLLAPDVPAVLLEMGFVNNPEDEARLRDPRSRARLMDSVADAIDDHFGHDMKVAAR

Sequences:

>Translated_382_residues
MAAGLGLAVAASHAAPDPVGLLKVRLGGDSAETRIVLDLAKSANVKIVSDGAADRRIVIHMPGVQAAEELQGPGRGLVKA
WMVDEVQGGARLQMDLAADAKVKRRFLLPPADGVEHYRYVVDVAAAPGASLTKASQAGPVDGALRTKFLPARKSALPLKK
VVVVDAGHGGKDVGAVGSKGYEKDINLAAAQALKDKLEKTGRYKVVMTRNSDVYIPLETRVRIAQRANADLFISLHSDSG
PDTSLKGASVYTLSDKASRRSTKFVSKDDWFMKASLTGDQGVRDILFDLTQRATRNRSAVFAQTLLSHIEDEAPLLRRSH
RDAGLVVLLAPDVPAVLLEMGFVNNPEDEARLRDPRSRARLMDSVADAIDDHFGHDMKVAAR
>Mature_381_residues
AAGLGLAVAASHAAPDPVGLLKVRLGGDSAETRIVLDLAKSANVKIVSDGAADRRIVIHMPGVQAAEELQGPGRGLVKAW
MVDEVQGGARLQMDLAADAKVKRRFLLPPADGVEHYRYVVDVAAAPGASLTKASQAGPVDGALRTKFLPARKSALPLKKV
VVVDAGHGGKDVGAVGSKGYEKDINLAAAQALKDKLEKTGRYKVVMTRNSDVYIPLETRVRIAQRANADLFISLHSDSGP
DTSLKGASVYTLSDKASRRSTKFVSKDDWFMKASLTGDQGVRDILFDLTQRATRNRSAVFAQTLLSHIEDEAPLLRRSHR
DAGLVVLLAPDVPAVLLEMGFVNNPEDEARLRDPRSRARLMDSVADAIDDHFGHDMKVAAR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=307, Percent_Identity=33.2247557003257, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1788776, Length=222, Percent_Identity=36.4864864864865, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1790611, Length=240, Percent_Identity=30.8333333333333, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 40957; Mature: 40826

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAGLGLAVAASHAAPDPVGLLKVRLGGDSAETRIVLDLAKSANVKIVSDGAADRRIVIH
CCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCEEEEE
MPGVQAAEELQGPGRGLVKAWMVDEVQGGARLQMDLAADAKVKRRFLLPPADGVEHYRYV
CCCCHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHEEECCCCCCHHHEEEE
VDVAAAPGASLTKASQAGPVDGALRTKFLPARKSALPLKKVVVVDAGHGGKDVGAVGSKG
EEEECCCCCCCCCCCCCCCCCCHHHHEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCCC
YEKDINLAAAQALKDKLEKTGRYKVVMTRNSDVYIPLETRVRIAQRANADLFISLHSDSG
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEEECCCC
PDTSLKGASVYTLSDKASRRSTKFVSKDDWFMKASLTGDQGVRDILFDLTQRATRNRSAV
CCCCCCCCEEEEECCCCCHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHH
FAQTLLSHIEDEAPLLRRSHRDAGLVVLLAPDVPAVLLEMGFVNNPEDEARLRDPRSRAR
HHHHHHHHHCCCCHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHCCHHHHHH
LMDSVADAIDDHFGHDMKVAAR
HHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
AAGLGLAVAASHAAPDPVGLLKVRLGGDSAETRIVLDLAKSANVKIVSDGAADRRIVIH
CCCCCCCEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCEEEEE
MPGVQAAEELQGPGRGLVKAWMVDEVQGGARLQMDLAADAKVKRRFLLPPADGVEHYRYV
CCCCHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHEEECCCCCCHHHEEEE
VDVAAAPGASLTKASQAGPVDGALRTKFLPARKSALPLKKVVVVDAGHGGKDVGAVGSKG
EEEECCCCCCCCCCCCCCCCCCHHHHEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCCC
YEKDINLAAAQALKDKLEKTGRYKVVMTRNSDVYIPLETRVRIAQRANADLFISLHSDSG
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEEECCCC
PDTSLKGASVYTLSDKASRRSTKFVSKDDWFMKASLTGDQGVRDILFDLTQRATRNRSAV
CCCCCCCCEEEEECCCCCHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHH
FAQTLLSHIEDEAPLLRRSHRDAGLVVLLAPDVPAVLLEMGFVNNPEDEARLRDPRSRAR
HHHHHHHHHCCCCHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHCCHHHHHH
LMDSVADAIDDHFGHDMKVAAR
HHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]