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The map label for this gene is prfB

Identifier: 197105302

GI number: 197105302

Start: 2058878

End: 2059987

Strand: Reverse

Name: prfB

Synonym: PHZ_c1839

Alternate gene names: 197105302

Gene position: 2059987-2058878 (Counterclockwise)

Preceding gene: 197105303

Following gene: 197105297

Centisome position: 51.55

GC content: 69.73

Gene sequence:

>1110_bases
ATGTCGAGGCCGCCAAGGCCGACATCGAGCAGTCGATCGAACTGCTCAGGAGGCGTCTTTGACTGGGAGCCAGCGCTCCG
GAAGCTCGACGAGCTGAACGCCCGGGTCGAGGACCCGACCCTTTGGAACGATCCTGACGAGGCCCAGGCGGTCTCGCGCG
AGCGTTCGCGCCTGTCGGCCCAGGTCGAAGCGGTCCATGGCCTCGAGCGGGACCTGGCCGACGCCCTCGGCTACGCCGAG
CTGGCGGACGAGGAGGGCGACGAGGCTTCGCTGGACGAGGCCCGCGCCCAGCTACGCGAGATCAAGGAGCGCGCCGCCCG
CGCCGAGCTGGAAGCCCTGCTGTCGGGCGAGGCAGACGGCAACGACGCCTATGTCGAGATCAACTCCGGCGCCGGCGGCA
CCGAGTCCAACGACTGGGCCGGCATGCTGCTGCGCATGTACACCCGCTGGGCCCAGGCCCACGGGATGAGCGTCGACGTG
ATCGAGGAGACGGCCGGGGAGCAGGCGGGGATCAAGTCCGTCACCCTGCAGGTGAAGGGGACCAACGCCTACGGCTGGCT
GAAGACCGAGGCCGGGGTTCACCGCCTCGTGCGCATCAGCCCGTATGATTCCAGCGCCCGGCGCCACACCTCCTTCGCCT
CGGTGTGGGTCTATCCGGTCATCGACGACAACATCGAGATCGAGATCAACCCGGCCGACGTGCGGACCGACACCTACCGG
GCCTCGGGCGCCGGCGGCCAGCACGTGAACAAGACCGACTCGGCGGTGCGCCTGACGCACATCCCGACAGGCATCGCCGT
GGCCTGCCAGACCCAGCGCTCGCAGCACCAGAACCGGGACCAGGCGTGGAAGATGCTGCGCGCCCGGCTCTATGAGCTGG
AGCTCGAGAAGCGCGAGGCCGCCCAGCAGGCGCTGGAGGACCAGAAGACCGACATCGGTTGGGGCCACCAGATCCGCAGC
TACGTGCTGCAGCCGTACCAGATGGTGAAGGACCTGCGGACCGAGGTGGAGACCTCCGACACCCAGGGCGTCCTGGACGG
CGACCTCGACGCGTTCATGGGGGCGGCGCTGGCCCAGCGGGTCGGCGCGACGCGGGACGCGGCGGCCTAG

Upstream 100 bases:

>100_bases
CTCCGCCGCCGAAGAAGCAGGACGAGCTGTCGGACTTGTTCTGACGCGGGACGCAGACTATGTCCGGCCGCCTGAAGTCC
GGAGTTCCCCATGAGAGTGG

Downstream 100 bases:

>100_bases
GCTATTCCGCGGCGATGTCGAGGCGCATCTGCGCCTCGTCCGCGGCCTCGGGAAACACCAGGCTGCGGCCCTCGAAATGG
GCGCCGGCCTCGATGGCCAG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSRPPRPTSSSRSNCSGGVFDWEPALRKLDELNARVEDPTLWNDPDEAQAVSRERSRLSAQVEAVHGLERDLADALGYAE
LADEEGDEASLDEARAQLREIKERAARAELEALLSGEADGNDAYVEINSGAGGTESNDWAGMLLRMYTRWAQAHGMSVDV
IEETAGEQAGIKSVTLQVKGTNAYGWLKTEAGVHRLVRISPYDSSARRHTSFASVWVYPVIDDNIEIEINPADVRTDTYR
ASGAGGQHVNKTDSAVRLTHIPTGIAVACQTQRSQHQNRDQAWKMLRARLYELELEKREAAQQALEDQKTDIGWGHQIRS
YVLQPYQMVKDLRTEVETSDTQGVLDGDLDAFMGAALAQRVGATRDAAA

Sequences:

>Translated_369_residues
MSRPPRPTSSSRSNCSGGVFDWEPALRKLDELNARVEDPTLWNDPDEAQAVSRERSRLSAQVEAVHGLERDLADALGYAE
LADEEGDEASLDEARAQLREIKERAARAELEALLSGEADGNDAYVEINSGAGGTESNDWAGMLLRMYTRWAQAHGMSVDV
IEETAGEQAGIKSVTLQVKGTNAYGWLKTEAGVHRLVRISPYDSSARRHTSFASVWVYPVIDDNIEIEINPADVRTDTYR
ASGAGGQHVNKTDSAVRLTHIPTGIAVACQTQRSQHQNRDQAWKMLRARLYELELEKREAAQQALEDQKTDIGWGHQIRS
YVLQPYQMVKDLRTEVETSDTQGVLDGDLDAFMGAALAQRVGATRDAAA
>Mature_368_residues
SRPPRPTSSSRSNCSGGVFDWEPALRKLDELNARVEDPTLWNDPDEAQAVSRERSRLSAQVEAVHGLERDLADALGYAEL
ADEEGDEASLDEARAQLREIKERAARAELEALLSGEADGNDAYVEINSGAGGTESNDWAGMLLRMYTRWAQAHGMSVDVI
EETAGEQAGIKSVTLQVKGTNAYGWLKTEAGVHRLVRISPYDSSARRHTSFASVWVYPVIDDNIEIEINPADVRTDTYRA
SGAGGQHVNKTDSAVRLTHIPTGIAVACQTQRSQHQNRDQAWKMLRARLYELELEKREAAQQALEDQKTDIGWGHQIRSY
VLQPYQMVKDLRTEVETSDTQGVLDGDLDAFMGAALAQRVGATRDAAA

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=297, Percent_Identity=35.3535353535354, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI34577120, Length=247, Percent_Identity=36.8421052631579, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI166795305, Length=211, Percent_Identity=36.0189573459716, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI2367172, Length=348, Percent_Identity=55.4597701149425, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1787462, Length=334, Percent_Identity=36.2275449101796, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=218, Percent_Identity=34.4036697247706, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=235, Percent_Identity=39.1489361702128, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19921226, Length=196, Percent_Identity=42.8571428571429, Blast_Score=154, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_PHEZH (B4RCR3)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130679.1
- ProteinModelPortal:   B4RCR3
- GeneID:   6791354
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1839
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- ProtClustDB:   PRK00578
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40678; Mature: 40547

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPPRPTSSSRSNCSGGVFDWEPALRKLDELNARVEDPTLWNDPDEAQAVSRERSRLSA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QVEAVHGLERDLADALGYAELADEEGDEASLDEARAQLREIKERAARAELEALLSGEADG
HHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NDAYVEINSGAGGTESNDWAGMLLRMYTRWAQAHGMSVDVIEETAGEQAGIKSVTLQVKG
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCEEEEEEEEC
TNAYGWLKTEAGVHRLVRISPYDSSARRHTSFASVWVYPVIDDNIEIEINPADVRTDTYR
CCCCEEEECHHCCEEEEEECCCCCCHHHHCCEEEEEEEEEECCCEEEEECCCCCCCCCEE
ASGAGGQHVNKTDSAVRLTHIPTGIAVACQTQRSQHQNRDQAWKMLRARLYELELEKREA
CCCCCCCCCCCCCCEEEEEECCCCEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AQQALEDQKTDIGWGHQIRSYVLQPYQMVKDLRTEVETSDTQGVLDGDLDAFMGAALAQR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
VGATRDAAA
HCCCCCCCC
>Mature Secondary Structure 
SRPPRPTSSSRSNCSGGVFDWEPALRKLDELNARVEDPTLWNDPDEAQAVSRERSRLSA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QVEAVHGLERDLADALGYAELADEEGDEASLDEARAQLREIKERAARAELEALLSGEADG
HHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NDAYVEINSGAGGTESNDWAGMLLRMYTRWAQAHGMSVDVIEETAGEQAGIKSVTLQVKG
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCEEEEEEEEC
TNAYGWLKTEAGVHRLVRISPYDSSARRHTSFASVWVYPVIDDNIEIEINPADVRTDTYR
CCCCEEEECHHCCEEEEEECCCCCCHHHHCCEEEEEEEEEECCCEEEEECCCCCCCCCEE
ASGAGGQHVNKTDSAVRLTHIPTGIAVACQTQRSQHQNRDQAWKMLRARLYELELEKREA
CCCCCCCCCCCCCCEEEEEECCCCEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AQQALEDQKTDIGWGHQIRSYVLQPYQMVKDLRTEVETSDTQGVLDGDLDAFMGAALAQR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
VGATRDAAA
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA