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The map label for this gene is nuoB [H]

Identifier: 197105267

GI number: 197105267

Start: 2018788

End: 2019369

Strand: Reverse

Name: nuoB [H]

Synonym: PHZ_c1804

Alternate gene names: 197105267

Gene position: 2019369-2018788 (Counterclockwise)

Preceding gene: 197105268

Following gene: 197105266

Centisome position: 50.53

GC content: 68.38

Gene sequence:

>582_bases
ATGGAAGAAAGGCGCCCTGGAATGGGAGTGATCGTTCCCGCGGGCGAGGCCGGCCGGACCACGGTCGAAGGCTACGACCC
CAAGCTCCATGACCCGTATTTCGACGGCGTCTCCCAGCGGCTGGCCGACAAGGGGTTCGTCACCGCCGCGGCCGACGACC
TGATCACCTGGGCCCGCACGGGCTCCCTGATGTGGATGACCTTCGGTCTCGCCTGCTGCGCGGTCGAGATGATGCAGGCG
TCCATGCCGCGCTACGACCTCGAGCGGTACGGCTTCGCCCCGCGCGCGAGCCCGCGCCAGTCGGACGTGATGATCGTCGC
CGGCACGCTGGTGAACAAGATGGCTCCGGCCCTGCGCAAGGTCTACGACCAGATGCCCGAGCCGCGCTACGTGATCTCGA
TGGGCAGCTGCGCCAACGGCGGCGGCTACTACTACTTCAGCTACTCCACGGTCCGCGGCTGCGACCGGGTGGTGCCGGTG
GACGTCTACGTCCCGGGCTGCCCGCCGACCGCCGAGGCCCTGGTCTACGGCGTGCTGCAGCTTCAGAAGAAGATCCGCCG
CACCGGGACGATCGTCCGATGA

Upstream 100 bases:

>100_bases
GGGCCGTGACGCTGATGAAGCTGCCGCCCGAGGCCAGCCAGTTCGCCTTCTGGTCGATGATGACCTTCCTGGGCGTGCTC
ACGGTGGGCTTCATCTACGA

Downstream 100 bases:

>100_bases
GCTGGCCCGCGACCCATGACGAGCTCGAGGCCCTGGGGCGTGAGCTGGTGGCCGGCGGCCTCGGCCCGTTCGGCGGCTAC
GAGGTGGCGTTCGGCGAGCT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MEERRPGMGVIVPAGEAGRTTVEGYDPKLHDPYFDGVSQRLADKGFVTAAADDLITWARTGSLMWMTFGLACCAVEMMQA
SMPRYDLERYGFAPRASPRQSDVMIVAGTLVNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYYFSYSTVRGCDRVVPV
DVYVPGCPPTAEALVYGVLQLQKKIRRTGTIVR

Sequences:

>Translated_193_residues
MEERRPGMGVIVPAGEAGRTTVEGYDPKLHDPYFDGVSQRLADKGFVTAAADDLITWARTGSLMWMTFGLACCAVEMMQA
SMPRYDLERYGFAPRASPRQSDVMIVAGTLVNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYYFSYSTVRGCDRVVPV
DVYVPGCPPTAEALVYGVLQLQKKIRRTGTIVR
>Mature_193_residues
MEERRPGMGVIVPAGEAGRTTVEGYDPKLHDPYFDGVSQRLADKGFVTAAADDLITWARTGSLMWMTFGLACCAVEMMQA
SMPRYDLERYGFAPRASPRQSDVMIVAGTLVNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYYFSYSTVRGCDRVVPV
DVYVPGCPPTAEALVYGVLQLQKKIRRTGTIVR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=157, Percent_Identity=71.9745222929936, Blast_Score=242, Evalue=1e-64,
Organism=Escherichia coli, GI1788624, Length=176, Percent_Identity=43.75, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789074, Length=126, Percent_Identity=38.8888888888889, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=38.2113821138211, Blast_Score=94, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=69.0140845070423, Blast_Score=220, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24651058, Length=190, Percent_Identity=62.1052631578947, Blast_Score=243, Evalue=7e-65,
Organism=Drosophila melanogaster, GI18859983, Length=168, Percent_Identity=67.2619047619048, Blast_Score=239, Evalue=6e-64,
Organism=Drosophila melanogaster, GI24642371, Length=168, Percent_Identity=67.2619047619048, Blast_Score=239, Evalue=6e-64,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 21270; Mature: 21270

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERRPGMGVIVPAGEAGRTTVEGYDPKLHDPYFDGVSQRLADKGFVTAAADDLITWART
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHC
GSLMWMTFGLACCAVEMMQASMPRYDLERYGFAPRASPRQSDVMIVAGTLVNKMAPALRK
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHH
VYDQMPEPRYVISMGSCANGGGYYYFSYSTVRGCDRVVPVDVYVPGCPPTAEALVYGVLQ
HHHHCCCCEEEEEECCCCCCCCEEEEEEHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHH
LQKKIRRTGTIVR
HHHHHHHHCCCCC
>Mature Secondary Structure
MEERRPGMGVIVPAGEAGRTTVEGYDPKLHDPYFDGVSQRLADKGFVTAAADDLITWART
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHC
GSLMWMTFGLACCAVEMMQASMPRYDLERYGFAPRASPRQSDVMIVAGTLVNKMAPALRK
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHH
VYDQMPEPRYVISMGSCANGGGYYYFSYSTVRGCDRVVPVDVYVPGCPPTAEALVYGVLQ
HHHHCCCCEEEEEECCCCCCCCEEEEEEHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHH
LQKKIRRTGTIVR
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA