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The map label for this gene is nuoE [H]

Identifier: 197105263

GI number: 197105263

Start: 2015910

End: 2016572

Strand: Reverse

Name: nuoE [H]

Synonym: PHZ_c1800

Alternate gene names: 197105263

Gene position: 2016572-2015910 (Counterclockwise)

Preceding gene: 197105264

Following gene: 197105262

Centisome position: 50.46

GC content: 68.78

Gene sequence:

>663_bases
GTGAGCGTCCGCAGGCTTTCCCCCGTCCAGCCCGAGAGCTTCGCCTTCAGCCCGGAGACGCTGAAGAAGGCGCAGGCCTG
GATGGCCAACTTCCCGCCCGGCAAGCAGCAGTCGGCCGTGGTGCCGGTCCTGTGGCTGGTCCAGAAGCAGGAAGGCTGGG
TCTCCGAGCCGGCCATCCGCGCGGTGGCCGAGCTGCTCGGCATGCCGGTGATCCGGGTGCTGGAGGTGGCGACCTTCTAC
ACCATGTTCATGCTGGAGCCGGTGGGCACCCATGCGCTGGTGCAGGTCTGCGGCACCACCCCGTGCCAGAGCCGCGGCGC
CGAAGCGCTGATGGAGGTCTGCAAGCGCCGCATCGGGCCGCAGAGCCACCGCTCGGCCGACGGCAAGTTCTACTGGCAGG
AAGTCGAGTGCCTGGGCGCCTGCGCCAACGCGCCGATGGCCGCCATCAACGACTACTACTACGAGGATCTGACGCCCGAG
AGCTTCGAGAAGCTGCTGGACGACTTCGCCGCCGGCAAGACCCCGCCGCCGGGCTCGGCCATCGGCCGCCAGTGCTCCGC
CCCCGAGGGCGGGCCCATGACGCTGACCGATCCGAAGCTCTACGACGGCTCGCTCGCCAAGAAGATCAAGATCCCGAACC
TGCCCTCGAAGGAGCCTGTGTGA

Upstream 100 bases:

>100_bases
ACCCGATCGCCCGGCGGCGAGACCTTCCACGTCGCCGCCATCTACACCCTCGCCGGCGGCAAGATCGCCCGCGTCGATTT
CGTGAAGTGAGGCCGAGACC

Downstream 100 bases:

>100_bases
TGCCGGGCATCCTGGCCGACAAGGACCGCATCTTCCTCAACCTCTACGGCCTGCACGACTGGGGCCTGGAGGGCGCGAAG
GCGCGCGGGGCGTGGAACGG

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 2; NDH-1, chain 2 [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSVRRLSPVQPESFAFSPETLKKAQAWMANFPPGKQQSAVVPVLWLVQKQEGWVSEPAIRAVAELLGMPVIRVLEVATFY
TMFMLEPVGTHALVQVCGTTPCQSRGAEALMEVCKRRIGPQSHRSADGKFYWQEVECLGACANAPMAAINDYYYEDLTPE
SFEKLLDDFAAGKTPPPGSAIGRQCSAPEGGPMTLTDPKLYDGSLAKKIKIPNLPSKEPV

Sequences:

>Translated_220_residues
MSVRRLSPVQPESFAFSPETLKKAQAWMANFPPGKQQSAVVPVLWLVQKQEGWVSEPAIRAVAELLGMPVIRVLEVATFY
TMFMLEPVGTHALVQVCGTTPCQSRGAEALMEVCKRRIGPQSHRSADGKFYWQEVECLGACANAPMAAINDYYYEDLTPE
SFEKLLDDFAAGKTPPPGSAIGRQCSAPEGGPMTLTDPKLYDGSLAKKIKIPNLPSKEPV
>Mature_219_residues
SVRRLSPVQPESFAFSPETLKKAQAWMANFPPGKQQSAVVPVLWLVQKQEGWVSEPAIRAVAELLGMPVIRVLEVATFYT
MFMLEPVGTHALVQVCGTTPCQSRGAEALMEVCKRRIGPQSHRSADGKFYWQEVECLGACANAPMAAINDYYYEDLTPES
FEKLLDDFAAGKTPPPGSAIGRQCSAPEGGPMTLTDPKLYDGSLAKKIKIPNLPSKEPV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=186, Percent_Identity=46.7741935483871, Blast_Score=177, Evalue=7e-45,
Organism=Escherichia coli, GI1788621, Length=161, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17561328, Length=178, Percent_Identity=46.0674157303371, Blast_Score=167, Evalue=4e-42,
Organism=Drosophila melanogaster, GI18859877, Length=185, Percent_Identity=47.027027027027, Blast_Score=180, Evalue=6e-46,
Organism=Drosophila melanogaster, GI21355353, Length=184, Percent_Identity=38.5869565217391, Blast_Score=144, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 24043; Mature: 23912

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRRLSPVQPESFAFSPETLKKAQAWMANFPPGKQQSAVVPVLWLVQKQEGWVSEPAIR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
AVAELLGMPVIRVLEVATFYTMFMLEPVGTHALVQVCGTTPCQSRGAEALMEVCKRRIGP
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
QSHRSADGKFYWQEVECLGACANAPMAAINDYYYEDLTPESFEKLLDDFAAGKTPPPGSA
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHH
IGRQCSAPEGGPMTLTDPKLYDGSLAKKIKIPNLPSKEPV
HCCCCCCCCCCCEECCCCCCCCCHHHHEEECCCCCCCCCC
>Mature Secondary Structure 
SVRRLSPVQPESFAFSPETLKKAQAWMANFPPGKQQSAVVPVLWLVQKQEGWVSEPAIR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
AVAELLGMPVIRVLEVATFYTMFMLEPVGTHALVQVCGTTPCQSRGAEALMEVCKRRIGP
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
QSHRSADGKFYWQEVECLGACANAPMAAINDYYYEDLTPESFEKLLDDFAAGKTPPPGSA
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHH
IGRQCSAPEGGPMTLTDPKLYDGSLAKKIKIPNLPSKEPV
HCCCCCCCCCCCEECCCCCCCCCHHHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1909571 [H]