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The map label for this gene is aceF [H]

Identifier: 197105204

GI number: 197105204

Start: 1950683

End: 1952023

Strand: Reverse

Name: aceF [H]

Synonym: PHZ_c1741

Alternate gene names: 197105204

Gene position: 1952023-1950683 (Counterclockwise)

Preceding gene: 197105205

Following gene: 197105203

Centisome position: 48.85

GC content: 70.99

Gene sequence:

>1341_bases
ATGACGGACATCCTCATGCCCGCCCTGTCTCCGACCATGGAGGAGGGCACCCTCGCCAAATGGCACGTGAAGCAGGGCGA
CGCCGTCCGCTCGGGCGACGTCATCGCCGAGATCGAGACCGACAAGGCCACCATGGAGGTCGAGGCGGTCGACGAAGGCG
TCGTCTCCGAGATCCTGGTGCCGGAAGGAACCGAGGGCGTGAAGGTGAACACGCCCATCGCCCGCCTGGGCGGCGAAGGC
GAGGCGGCCGCTCCGGCGCCGCAGCCGAAGGCCGAGGCGCCGAAGCCCGCGCCGACCCCCGAGAGCGACGGCGCCCGCGC
CGCCCGCGAGGAGAAGACCGAGGCCGCGGCCAAAACTCCGGCCCAGGCTCCCGCGCCGGCCCCCTCGCCGGCCAGGGCGC
AGGACGGCTCGCGGATCTTCGCCTCGCCTCTTGCCCGCCGGCTGGCCGAGCAGAAGGGCGTCGACCTCTCGGCGGTGAAG
GGCACCGGGCCGCACGGCCGGATCGTCAAGGCCGACATCGAGCAAGCCCGGCCTGGCGAGACCAAGCCCGGTGAAGCGAA
GGCGCCGGCGGCCCAGCCGACCGCTGCGCCCTCGGCGGCGCGCGCCGAACCGCGCCCGGCGGCGAGCCTGGAGCAGATGG
GTATCGCGCCCGGCAGCTACGACCTCATCCCGCTGGACGGCATGCGCAAGACCGTCGCCCGGCGGATGACCGACAGCTTC
CGGGACGTGCCGCACTTCCCGCTGACCATCGACCTCGAGATCGACGGCCTGCTGGCCGCCCGCGCCCGGATCAACGCTTT
GCTCGAGAAGGAAGGCGTGAAAGTCAGCGTCAACGACATGGTGATGAAGGCCGCGGCCGTGGCCCTGAAGCGCGTGCCCG
AGGCGAACGCCTCCTACACGCCCGAGGGCATCGCCATGCACCACCACGCCGACATCGCCATGGCCGTGGCGGTCCCGGGG
GGCCTGATCACGCCGATCATCCGCAAGGCCGAGACCAAGGGCCTGGCGCAGATCGCCACCGAGGCCAAGGACCTGGCCGA
GCGGGCGCGGAACAAGAAGCTGAAGCCCGAGGAATTCCAAGGCGGCACCTTCAGCGTCTCCAATCTCGGGATGTTCGGCA
TCAAGACGTTCTCCTCGATCCTCAACGAGCCGCAGGGCTGCATCCTGTCGGTCGGCGCGGGCGAGAAGCGGCCCGTGGTG
CGCGGCGACAAGCTGGAGATCGCCACGCTGATGTCCGTGACCCTGACCTGCGACCACCGGGTCGTGGACGGGGCGACCGG
CGCGCGCTGGCTGCAGGCGTTCAAGGCGCTGATCGAAGAGCCGCTGACGATGATCGTCTAG

Upstream 100 bases:

>100_bases
TCGATACGGTCGAGGGACTGGAGATCGCGATCGAAGCCTTCGACGGCCAGAACTGGGAGGCGAACGCCGCCGGCCTGGCC
CATCTGTCCAAGGAACCCGC

Downstream 100 bases:

>100_bases
GCGGGAGGGTCCGGGATGGCTTCCATCTACGACTTCACCGCCGAGACCATCGACGGCGCGCCGGCGCCGCTCGCGGACCA
CCGCGGCAAGGTGCTGCTGA

Product: pyruvate dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTDILMPALSPTMEEGTLAKWHVKQGDAVRSGDVIAEIETDKATMEVEAVDEGVVSEILVPEGTEGVKVNTPIARLGGEG
EAAAPAPQPKAEAPKPAPTPESDGARAAREEKTEAAAKTPAQAPAPAPSPARAQDGSRIFASPLARRLAEQKGVDLSAVK
GTGPHGRIVKADIEQARPGETKPGEAKAPAAQPTAAPSAARAEPRPAASLEQMGIAPGSYDLIPLDGMRKTVARRMTDSF
RDVPHFPLTIDLEIDGLLAARARINALLEKEGVKVSVNDMVMKAAAVALKRVPEANASYTPEGIAMHHHADIAMAVAVPG
GLITPIIRKAETKGLAQIATEAKDLAERARNKKLKPEEFQGGTFSVSNLGMFGIKTFSSILNEPQGCILSVGAGEKRPVV
RGDKLEIATLMSVTLTCDHRVVDGATGARWLQAFKALIEEPLTMIV

Sequences:

>Translated_446_residues
MTDILMPALSPTMEEGTLAKWHVKQGDAVRSGDVIAEIETDKATMEVEAVDEGVVSEILVPEGTEGVKVNTPIARLGGEG
EAAAPAPQPKAEAPKPAPTPESDGARAAREEKTEAAAKTPAQAPAPAPSPARAQDGSRIFASPLARRLAEQKGVDLSAVK
GTGPHGRIVKADIEQARPGETKPGEAKAPAAQPTAAPSAARAEPRPAASLEQMGIAPGSYDLIPLDGMRKTVARRMTDSF
RDVPHFPLTIDLEIDGLLAARARINALLEKEGVKVSVNDMVMKAAAVALKRVPEANASYTPEGIAMHHHADIAMAVAVPG
GLITPIIRKAETKGLAQIATEAKDLAERARNKKLKPEEFQGGTFSVSNLGMFGIKTFSSILNEPQGCILSVGAGEKRPVV
RGDKLEIATLMSVTLTCDHRVVDGATGARWLQAFKALIEEPLTMIV
>Mature_445_residues
TDILMPALSPTMEEGTLAKWHVKQGDAVRSGDVIAEIETDKATMEVEAVDEGVVSEILVPEGTEGVKVNTPIARLGGEGE
AAAPAPQPKAEAPKPAPTPESDGARAAREEKTEAAAKTPAQAPAPAPSPARAQDGSRIFASPLARRLAEQKGVDLSAVKG
TGPHGRIVKADIEQARPGETKPGEAKAPAAQPTAAPSAARAEPRPAASLEQMGIAPGSYDLIPLDGMRKTVARRMTDSFR
DVPHFPLTIDLEIDGLLAARARINALLEKEGVKVSVNDMVMKAAAVALKRVPEANASYTPEGIAMHHHADIAMAVAVPGG
LITPIIRKAETKGLAQIATEAKDLAERARNKKLKPEEFQGGTFSVSNLGMFGIKTFSSILNEPQGCILSVGAGEKRPVVR
GDKLEIATLMSVTLTCDHRVVDGATGARWLQAFKALIEEPLTMIV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=451, Percent_Identity=43.0155210643016, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI203098753, Length=461, Percent_Identity=37.527114967462, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI203098816, Length=461, Percent_Identity=37.527114967462, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI260898739, Length=204, Percent_Identity=40.6862745098039, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI110671329, Length=441, Percent_Identity=25.8503401360544, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI1786946, Length=445, Percent_Identity=32.1348314606742, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1786305, Length=413, Percent_Identity=31.7191283292978, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=475, Percent_Identity=41.2631578947368, Blast_Score=324, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI17538894, Length=313, Percent_Identity=37.3801916932907, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=438, Percent_Identity=28.0821917808219, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=457, Percent_Identity=29.7592997811816, Blast_Score=164, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6324258, Length=455, Percent_Identity=40.4395604395604, Blast_Score=288, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6320352, Length=448, Percent_Identity=26.7857142857143, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321632, Length=262, Percent_Identity=27.4809160305344, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20129315, Length=451, Percent_Identity=38.5809312638581, Blast_Score=281, Evalue=8e-76,
Organism=Drosophila melanogaster, GI24582497, Length=444, Percent_Identity=37.8378378378378, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI18859875, Length=444, Percent_Identity=29.5045045045045, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=32.7433628318584, Blast_Score=122, Evalue=7e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46853; Mature: 46722

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDILMPALSPTMEEGTLAKWHVKQGDAVRSGDVIAEIETDKATMEVEAVDEGVVSEILV
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHCC
PEGTEGVKVNTPIARLGGEGEAAAPAPQPKAEAPKPAPTPESDGARAAREEKTEAAAKTP
CCCCCCEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
AQAPAPAPSPARAQDGSRIFASPLARRLAEQKGVDLSAVKGTGPHGRIVKADIEQARPGE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECHHHCCCCC
TKPGEAKAPAAQPTAAPSAARAEPRPAASLEQMGIAPGSYDLIPLDGMRKTVARRMTDSF
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHH
RDVPHFPLTIDLEIDGLLAARARINALLEKEGVKVSVNDMVMKAAAVALKRVPEANASYT
HCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCC
PEGIAMHHHADIAMAVAVPGGLITPIIRKAETKGLAQIATEAKDLAERARNKKLKPEEFQ
CCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCC
GGTFSVSNLGMFGIKTFSSILNEPQGCILSVGAGEKRPVVRGDKLEIATLMSVTLTCDHR
CCEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEEEEECCE
VVDGATGARWLQAFKALIEEPLTMIV
EECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDILMPALSPTMEEGTLAKWHVKQGDAVRSGDVIAEIETDKATMEVEAVDEGVVSEILV
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHCC
PEGTEGVKVNTPIARLGGEGEAAAPAPQPKAEAPKPAPTPESDGARAAREEKTEAAAKTP
CCCCCCEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
AQAPAPAPSPARAQDGSRIFASPLARRLAEQKGVDLSAVKGTGPHGRIVKADIEQARPGE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECHHHCCCCC
TKPGEAKAPAAQPTAAPSAARAEPRPAASLEQMGIAPGSYDLIPLDGMRKTVARRMTDSF
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHH
RDVPHFPLTIDLEIDGLLAARARINALLEKEGVKVSVNDMVMKAAAVALKRVPEANASYT
HCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCC
PEGIAMHHHADIAMAVAVPGGLITPIIRKAETKGLAQIATEAKDLAERARNKKLKPEEFQ
CCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCC
GGTFSVSNLGMFGIKTFSSILNEPQGCILSVGAGEKRPVVRGDKLEIATLMSVTLTCDHR
CCEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEEEEECCE
VVDGATGARWLQAFKALIEEPLTMIV
EECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]