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The map label for this gene is glnA [H]

Identifier: 197105035

GI number: 197105035

Start: 1774043

End: 1775452

Strand: Reverse

Name: glnA [H]

Synonym: PHZ_c1572

Alternate gene names: 197105035

Gene position: 1775452-1774043 (Counterclockwise)

Preceding gene: 197105036

Following gene: 197105033

Centisome position: 44.43

GC content: 64.4

Gene sequence:

>1410_bases
ATGGCTTCCGCCCAGGACATCCTGAACCTGATCAAGGAAAAGGACGTCAAATACGTCGACGTCCGCTTCACCGACATCCG
CGGGAAGATGCAGCACGTCACCTTCGACATCGACCTCGTCGACGAGGACTTCCTCACCGACGGCACGATGTTCGACGGCT
CGTCCATCGCCGGCTGGAAGGCCATCAACGAGTCCGACATGAAGCTGCGGCCGGACCTGGACTCGGCGATCATCGACCCG
TTCTACCAGCAGACCACGCTCGCCCTGTTCTGCGACATCGTGAACCCCGACACCAACACGCCCTACAACCGCGATCCCCG
CTCGATCGCCAAGGCGGCGCTGTCCTACGTCAAGGCCGCCGGCGTGGGCGACACCGTGTTCTTCGGTCCCGAGGCCGAAT
TCTTCGTCTTCGACGACGTCCAGTGGAACACCGCCGCGCACGACACGCGCTACTCCTACGACTCCACCGAGCTGCCGGTG
AATTCGGGCAAGTCCTATCCCGAGGGCAACATGGGCCACCGCCCGGGCCCCAAGGGCGGGTACTTCCCCGTCAATCCGGT
GGACAGCGGCCAGGACCTGCGCGGCGAGATGCTGGCGGTCATGGGCGAGCTCGGCATGAAGCCCGAGAAGCACCACCACG
AGGTGGCGCCGGCCCAGCACGAGCTCGGCCTGAAGTTCGCCGAGCTCATCACCATGGCCGACCGGATGCAGCTCTATAAG
TACGTGATCCACAACGTGGCCCACGCCTACGGCAAGACCGCCACCTTCATGGCCAAGCCGATGTTCGGCGACAACGGCTC
GGGCATGCACGTCCACCAGTCGATCTGGAAGGACGGCAAGCCCATGTTCGCGGGCGACAAGTACGCCGGCCTGTCCGAGA
TGTGCCTCTGGTACATCGGCGGCATCATCAAGCACGCCAAGGCGATCAACGCCTTCTCGAACTCGACCACGAACTCGTAC
AAGCGCCTGGTGCCCGGCTACGAGGCGCCGGTGAAGCTGGCCTACTCGGCCCGCAACCGCTCGGCCTCGATCCGCATCCC
GCACGTGGACTCGCCGAAGGCCAAGCGCATCGAGGCCCGCTTCCCGGACCCGATGGGCAACCCCTACCTCACGTTCGTGG
CGCTGCTGATGGCCGGCCTCGACGGCATCGAGAACAAGATCGATCCCGGCCCGGCCATGGACAAGAACCTCTACGACCTG
CCCCCGCGCGAGCAGAAGAAGGTCCCCGAGGTCTGCGGCAGCCTGCGCGAGGCCCTCGAGAACCTCGACAAGGACCGCGC
CTTCCTCAAGAAGGGCGGCGTCATGGACGACGACTTCATCGACGCCTACATCGAGCTGAAGATGGAGGAGGTCATGCGCC
TCCAGCTCCATCCGCACCCGGTGGAATTCGACATGTACTTCAAGTGCTAG

Upstream 100 bases:

>100_bases
TCATGCGGATCCGGACCGGCGAGACCGGACCCGTCGCCATCTGACCTCAACGACACCCGCCAAAAGCAACCGATCGAGAC
CAAGCCAAGGGGATAACAAC

Downstream 100 bases:

>100_bases
TCGGGATTTCCTGACCGAACAGGCAAGGGCCGCGGAGCGATCCGCGGCCCTTTTCTTGTCGGCTCCGCGTCAGGCCGGCT
TGCAGGCGCCGGTCGAGGCG

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MASAQDILNLIKEKDVKYVDVRFTDIRGKMQHVTFDIDLVDEDFLTDGTMFDGSSIAGWKAINESDMKLRPDLDSAIIDP
FYQQTTLALFCDIVNPDTNTPYNRDPRSIAKAALSYVKAAGVGDTVFFGPEAEFFVFDDVQWNTAAHDTRYSYDSTELPV
NSGKSYPEGNMGHRPGPKGGYFPVNPVDSGQDLRGEMLAVMGELGMKPEKHHHEVAPAQHELGLKFAELITMADRMQLYK
YVIHNVAHAYGKTATFMAKPMFGDNGSGMHVHQSIWKDGKPMFAGDKYAGLSEMCLWYIGGIIKHAKAINAFSNSTTNSY
KRLVPGYEAPVKLAYSARNRSASIRIPHVDSPKAKRIEARFPDPMGNPYLTFVALLMAGLDGIENKIDPGPAMDKNLYDL
PPREQKKVPEVCGSLREALENLDKDRAFLKKGGVMDDDFIDAYIELKMEEVMRLQLHPHPVEFDMYFKC

Sequences:

>Translated_469_residues
MASAQDILNLIKEKDVKYVDVRFTDIRGKMQHVTFDIDLVDEDFLTDGTMFDGSSIAGWKAINESDMKLRPDLDSAIIDP
FYQQTTLALFCDIVNPDTNTPYNRDPRSIAKAALSYVKAAGVGDTVFFGPEAEFFVFDDVQWNTAAHDTRYSYDSTELPV
NSGKSYPEGNMGHRPGPKGGYFPVNPVDSGQDLRGEMLAVMGELGMKPEKHHHEVAPAQHELGLKFAELITMADRMQLYK
YVIHNVAHAYGKTATFMAKPMFGDNGSGMHVHQSIWKDGKPMFAGDKYAGLSEMCLWYIGGIIKHAKAINAFSNSTTNSY
KRLVPGYEAPVKLAYSARNRSASIRIPHVDSPKAKRIEARFPDPMGNPYLTFVALLMAGLDGIENKIDPGPAMDKNLYDL
PPREQKKVPEVCGSLREALENLDKDRAFLKKGGVMDDDFIDAYIELKMEEVMRLQLHPHPVEFDMYFKC
>Mature_468_residues
ASAQDILNLIKEKDVKYVDVRFTDIRGKMQHVTFDIDLVDEDFLTDGTMFDGSSIAGWKAINESDMKLRPDLDSAIIDPF
YQQTTLALFCDIVNPDTNTPYNRDPRSIAKAALSYVKAAGVGDTVFFGPEAEFFVFDDVQWNTAAHDTRYSYDSTELPVN
SGKSYPEGNMGHRPGPKGGYFPVNPVDSGQDLRGEMLAVMGELGMKPEKHHHEVAPAQHELGLKFAELITMADRMQLYKY
VIHNVAHAYGKTATFMAKPMFGDNGSGMHVHQSIWKDGKPMFAGDKYAGLSEMCLWYIGGIIKHAKAINAFSNSTTNSYK
RLVPGYEAPVKLAYSARNRSASIRIPHVDSPKAKRIEARFPDPMGNPYLTFVALLMAGLDGIENKIDPGPAMDKNLYDLP
PREQKKVPEVCGSLREALENLDKDRAFLKKGGVMDDDFIDAYIELKMEEVMRLQLHPHPVEFDMYFKC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=62.1794871794872, Blast_Score=609, Evalue=1e-175,
Organism=Escherichia coli, GI87081870, Length=389, Percent_Identity=23.1362467866324, Blast_Score=82, Evalue=9e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52532; Mature: 52401

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAQDILNLIKEKDVKYVDVRFTDIRGKMQHVTFDIDLVDEDFLTDGTMFDGSSIAGWK
CCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCEECCCCCCCCC
AINESDMKLRPDLDSAIIDPFYQQTTLALFCDIVNPDTNTPYNRDPRSIAKAALSYVKAA
CCCCCCCEECCCCCHHHHCHHHHHHEEHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
GVGDTVFFGPEAEFFVFDDVQWNTAAHDTRYSYDSTELPVNSGKSYPEGNMGHRPGPKGG
CCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVNPVDSGQDLRGEMLAVMGELGMKPEKHHHEVAPAQHELGLKFAELITMADRMQLYK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHCHHHHHHHHHHHHHHHHH
YVIHNVAHAYGKTATFMAKPMFGDNGSGMHVHQSIWKDGKPMFAGDKYAGLSEMCLWYIG
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
GIIKHAKAINAFSNSTTNSYKRLVPGYEAPVKLAYSARNRSASIRIPHVDSPKAKRIEAR
HHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCHHEEECC
FPDPMGNPYLTFVALLMAGLDGIENKIDPGPAMDKNLYDLPPREQKKVPEVCGSLREALE
CCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NLDKDRAFLKKGGVMDDDFIDAYIELKMEEVMRLQLHPHPVEFDMYFKC
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
>Mature Secondary Structure 
ASAQDILNLIKEKDVKYVDVRFTDIRGKMQHVTFDIDLVDEDFLTDGTMFDGSSIAGWK
CCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCEECCCCCCCCC
AINESDMKLRPDLDSAIIDPFYQQTTLALFCDIVNPDTNTPYNRDPRSIAKAALSYVKAA
CCCCCCCEECCCCCHHHHCHHHHHHEEHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
GVGDTVFFGPEAEFFVFDDVQWNTAAHDTRYSYDSTELPVNSGKSYPEGNMGHRPGPKGG
CCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVNPVDSGQDLRGEMLAVMGELGMKPEKHHHEVAPAQHELGLKFAELITMADRMQLYK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHCHHHHHHHHHHHHHHHHH
YVIHNVAHAYGKTATFMAKPMFGDNGSGMHVHQSIWKDGKPMFAGDKYAGLSEMCLWYIG
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
GIIKHAKAINAFSNSTTNSYKRLVPGYEAPVKLAYSARNRSASIRIPHVDSPKAKRIEAR
HHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCHHEEECC
FPDPMGNPYLTFVALLMAGLDGIENKIDPGPAMDKNLYDLPPREQKKVPEVCGSLREALE
CCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NLDKDRAFLKKGGVMDDDFIDAYIELKMEEVMRLQLHPHPVEFDMYFKC
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275; 2859270; 2793830 [H]