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The map label for this gene is nccH [H]

Identifier: 197104994

GI number: 197104994

Start: 1732219

End: 1732803

Strand: Direct

Name: nccH [H]

Synonym: PHZ_c1531

Alternate gene names: 197104994

Gene position: 1732219-1732803 (Clockwise)

Preceding gene: 197104993

Following gene: 197104995

Centisome position: 43.35

GC content: 71.62

Gene sequence:

>585_bases
ATGCCGGGCACGAAACCATCGAGCTTCGCCAGCCTGCACGACGTCGAGCTCTGCTCGCACGCCGCGGCCGGCGAGGGGCG
CGCCTTCGGGGAGCTTGCGCGCCGCCACGGCTCGGCCGTCCGCTACCTGCTGCGCCGGATGGGCGCCCAGGCGGCTGAGG
CCGACGACGTGGCCCAGGACGCCTTCCTCACCGCCTTCCAGCGGATCACCGAGTTCCGCGGCGAGGGGACCTTCGCCGCC
TGGGTGAAGCGGATCGCCGCGCGCCATTATCTGCGCCGGCTCCAGCGCGACCGGCGGCTGAGCGAGCTGGCGGCGGAGGG
GGGCGAAGAGGAGATCGAGATCGCGGCGGGCGACGCAGACCACCGGATCGACCTGGACGAGGCCCTGAAGGCGCTCTCCA
AGGCCGAGCGGCTGTGCGTGTCGCTGTGCTTCGGCGCGGGCCTTTCCCACGGCGAGGCGGCCGAGGCCTTGAACCTGCCG
CTTGGAACGGTCAAATCCCATGTCAAACGTGGTCTGGATAAGCTCAGAGCGCGACTGGCGCCGACCGAGGGCGCCGTCCT
GGAAGGGAGGCGGGGCAATGGCTGA

Upstream 100 bases:

>100_bases
CATCGCCTCGTTCCCGGGCTTCATCGGCCTGGCCTTCATCGTCCTCGGCCTGATCAACAAGCCGAAGTCCTAGAACCCTC
CTAAAGGGGCATAGCCGCCC

Downstream 100 bases:

>100_bases
CGATTTCGAACGCCGCCTCGAGCGCCTGTTCTCCGAAGCGCCGGAGCTGCCGGATTGCGCGGCCTTCGCGCAGGGCGTCG
AGCGTAAGCTGAACCGCGGC

Product: RNA polymerase sigma-70 factor, ECF subfamily

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MPGTKPSSFASLHDVELCSHAAAGEGRAFGELARRHGSAVRYLLRRMGAQAAEADDVAQDAFLTAFQRITEFRGEGTFAA
WVKRIAARHYLRRLQRDRRLSELAAEGGEEEIEIAAGDADHRIDLDEALKALSKAERLCVSLCFGAGLSHGEAAEALNLP
LGTVKSHVKRGLDKLRARLAPTEGAVLEGRRGNG

Sequences:

>Translated_194_residues
MPGTKPSSFASLHDVELCSHAAAGEGRAFGELARRHGSAVRYLLRRMGAQAAEADDVAQDAFLTAFQRITEFRGEGTFAA
WVKRIAARHYLRRLQRDRRLSELAAEGGEEEIEIAAGDADHRIDLDEALKALSKAERLCVSLCFGAGLSHGEAAEALNLP
LGTVKSHVKRGLDKLRARLAPTEGAVLEGRRGNG
>Mature_193_residues
PGTKPSSFASLHDVELCSHAAAGEGRAFGELARRHGSAVRYLLRRMGAQAAEADDVAQDAFLTAFQRITEFRGEGTFAAW
VKRIAARHYLRRLQRDRRLSELAAEGGEEEIEIAAGDADHRIDLDEALKALSKAERLCVSLCFGAGLSHGEAAEALNLPL
GTVKSHVKRGLDKLRARLAPTEGAVLEGRRGNG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates the genes for a membrane-located efflux system that confers resistance to nickel, cobalt and

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=187, Percent_Identity=27.807486631016, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 20956; Mature: 20825

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTKPSSFASLHDVELCSHAAAGEGRAFGELARRHGSAVRYLLRRMGAQAAEADDVAQD
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AFLTAFQRITEFRGEGTFAAWVKRIAARHYLRRLQRDRRLSELAAEGGEEEIEIAAGDAD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
HRIDLDEALKALSKAERLCVSLCFGAGLSHGEAAEALNLPLGTVKSHVKRGLDKLRARLA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PTEGAVLEGRRGNG
CCCCCEECCCCCCC
>Mature Secondary Structure 
PGTKPSSFASLHDVELCSHAAAGEGRAFGELARRHGSAVRYLLRRMGAQAAEADDVAQD
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AFLTAFQRITEFRGEGTFAAWVKRIAARHYLRRLQRDRRLSELAAEGGEEEIEIAAGDAD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
HRIDLDEALKALSKAERLCVSLCFGAGLSHGEAAEALNLPLGTVKSHVKRGLDKLRARLA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PTEGAVLEGRRGNG
CCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961470 [H]