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The map label for this gene is tetA [H]

Identifier: 197104915

GI number: 197104915

Start: 1646591

End: 1647853

Strand: Reverse

Name: tetA [H]

Synonym: PHZ_c1450

Alternate gene names: 197104915

Gene position: 1647853-1646591 (Counterclockwise)

Preceding gene: 197104916

Following gene: 197104904

Centisome position: 41.23

GC content: 73.63

Gene sequence:

>1263_bases
ATGCCCCCGCTGACCGGCGCTCGAGGCGCATCCTGGAGGCCGGGCTTTCCACACCCGGTCTTCGTCGTGGCGGCGCTCGA
CGCCATGTCCGTCGGCATCATCGTCCCGGCGCTGCCGCATCTGGTGGAGCAACTCGCCGGCTCGGACGGCGCGGACCCGG
CCGTGTGGCTCGCCCTCCTCGCCGCCGCCTGGGGCGCCGCGCAGTTCCTGAGCGCGCCCATCATGGGCGTCTTGGCGGAT
CGGTTCGGACGCCGCCCAGTGCTCCTGGCGGCCCTGGCCGGACTGGCGTGCGACGCCCTGTTCATGGCCCTGGCGCCCAG
TCTCGGCTGGCTTCTCGTCGGACGCCTGATCAGCGGAGCGCTCGCGGCCAGCATCATCGCCTGCAGCGCCTACATCATCG
ACGTGAGCGCAGGCGACGAACTGGCCCGCCGGCTCGGCCTGCTGGGCGCGGCCTGGTCAGCCGGGTTCATTGCGGGTCCC
GCCCTGGGGGGCGCCTTGGCCGACCTTCACCTGCGTCTTCCGTTCCTGGTGTGCGGCGCATCCGCCGCCGTGGCCTGGCT
CTACGCGCGCTTTCGCCTCAAGGAGTCGCTGCCCATAGAGCGGCGCAGCACGGGCCTCGTGTGGCGGCGCGCCGTCCCCT
TCGGTGGGCTGACCGCGCTTCTTCGGCGCCGCGCCCTCAGGACGCATGGGCTTGTGCTGGCGCTCGCCGCCCTGGCCGAC
CAGGCCTTGCCCACATTCTTCGTCCTCTACGTGCTCGAGCGGTATGGCTGGAGCGCCAGCGCCGCGGGCCTGGCGCTCAT
GGGCCTGGGCGCTGCGCGCGTCTTGGCCCAGGCGGTCGCCGTGGCCCCCCTGACCCGGCGCTACGGCGAGCCGGCCACAC
TGGTGTTCGGCCTCTTATGCCTGATGCTGGCGCTGGTGGTCTTTGCGGTCGCGCCCACGGGGCTCGTCTTCGGGCTGGCG
CTGCCGATCTACGCCGCCGGCGCGGTCGCCGCCCCGGCGCTCATGGCGATTCTGGCCGGCAGGGTGGAGCGCGAGCAGCA
AGGCCGGCTGCAAGGGGCGAGCGTCGCCGTCCAGGGAGCGGCGAGCGCGGTGGGTCCCCTCCTCTTCGGGGCCGCCTATG
CCCTGAATTCGCAGAACGGGGTTCCACACCGTGGCGCTGGCGCGATGCTCCTCGGCGCCGCGCTTCTCGCAGTGGCGGCC
ACGATCGCGCACGCCGCCGCGCGAGTTCCGCCGCGCATGCCGCGCGCGCGGTCCGAGCCGTGA

Upstream 100 bases:

>100_bases
CCTGGCCTCGGAGCTCGACCGCATCCTCGACGCGGCCCGCCAGCATGGCCTGAGCGACGACGGATGCCTCCTGTTTCGCG
GCTTCCGTCCCGCCTAGGCG

Downstream 100 bases:

>100_bases
GCCGCAGATTGGAACGCCGGAGACTATCCTGTGCCCCCTCGCGATGCGCATGCCGCCGGCGGCGGCGTGGGAAAAGGTCG
GGCTCGCGCCCGGCGGGGGA

Product: tetracycline resistance protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: TetA(A) [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MPPLTGARGASWRPGFPHPVFVVAALDAMSVGIIVPALPHLVEQLAGSDGADPAVWLALLAAAWGAAQFLSAPIMGVLAD
RFGRRPVLLAALAGLACDALFMALAPSLGWLLVGRLISGALAASIIACSAYIIDVSAGDELARRLGLLGAAWSAGFIAGP
ALGGALADLHLRLPFLVCGASAAVAWLYARFRLKESLPIERRSTGLVWRRAVPFGGLTALLRRRALRTHGLVLALAALAD
QALPTFFVLYVLERYGWSASAAGLALMGLGAARVLAQAVAVAPLTRRYGEPATLVFGLLCLMLALVVFAVAPTGLVFGLA
LPIYAAGAVAAPALMAILAGRVEREQQGRLQGASVAVQGAASAVGPLLFGAAYALNSQNGVPHRGAGAMLLGAALLAVAA
TIAHAAARVPPRMPRARSEP

Sequences:

>Translated_420_residues
MPPLTGARGASWRPGFPHPVFVVAALDAMSVGIIVPALPHLVEQLAGSDGADPAVWLALLAAAWGAAQFLSAPIMGVLAD
RFGRRPVLLAALAGLACDALFMALAPSLGWLLVGRLISGALAASIIACSAYIIDVSAGDELARRLGLLGAAWSAGFIAGP
ALGGALADLHLRLPFLVCGASAAVAWLYARFRLKESLPIERRSTGLVWRRAVPFGGLTALLRRRALRTHGLVLALAALAD
QALPTFFVLYVLERYGWSASAAGLALMGLGAARVLAQAVAVAPLTRRYGEPATLVFGLLCLMLALVVFAVAPTGLVFGLA
LPIYAAGAVAAPALMAILAGRVEREQQGRLQGASVAVQGAASAVGPLLFGAAYALNSQNGVPHRGAGAMLLGAALLAVAA
TIAHAAARVPPRMPRARSEP
>Mature_419_residues
PPLTGARGASWRPGFPHPVFVVAALDAMSVGIIVPALPHLVEQLAGSDGADPAVWLALLAAAWGAAQFLSAPIMGVLADR
FGRRPVLLAALAGLACDALFMALAPSLGWLLVGRLISGALAASIIACSAYIIDVSAGDELARRLGLLGAAWSAGFIAGPA
LGGALADLHLRLPFLVCGASAAVAWLYARFRLKESLPIERRSTGLVWRRAVPFGGLTALLRRRALRTHGLVLALAALADQ
ALPTFFVLYVLERYGWSASAAGLALMGLGAARVLAQAVAVAPLTRRYGEPATLVFGLLCLMLALVVFAVAPTGLVFGLAL
PIYAAGAVAAPALMAILAGRVEREQQGRLQGASVAVQGAASAVGPLLFGAAYALNSQNGVPHRGAGAMLLGAALLAVAAT
IAHAAARVPPRMPRARSEP

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI94721311, Length=402, Percent_Identity=25.3731343283582, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI39753965, Length=332, Percent_Identity=27.1084337349398, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI225703104, Length=344, Percent_Identity=29.0697674418605, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI225703102, Length=344, Percent_Identity=29.0697674418605, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71996331, Length=337, Percent_Identity=24.0356083086053, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21357569, Length=336, Percent_Identity=25, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI221513297, Length=330, Percent_Identity=26.0606060606061, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24656463, Length=347, Percent_Identity=24.7838616714697, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24656468, Length=347, Percent_Identity=24.7838616714697, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI161080926, Length=347, Percent_Identity=24.7838616714697, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI161080924, Length=347, Percent_Identity=24.7838616714697, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17738085, Length=353, Percent_Identity=25.4957507082153, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24652013, Length=327, Percent_Identity=25.0764525993884, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42964; Mature: 42833

Theoretical pI: Translated: 11.27; Mature: 11.27

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLTGARGASWRPGFPHPVFVVAALDAMSVGIIVPALPHLVEQLAGSDGADPAVWLALL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AAAWGAAQFLSAPIMGVLADRFGRRPVLLAALAGLACDALFMALAPSLGWLLVGRLISGA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAASIIACSAYIIDVSAGDELARRLGLLGAAWSAGFIAGPALGGALADLHLRLPFLVCGA
HHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
SAAVAWLYARFRLKESLPIERRSTGLVWRRAVPFGGLTALLRRRALRTHGLVLALAALAD
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QALPTFFVLYVLERYGWSASAAGLALMGLGAARVLAQAVAVAPLTRRYGEPATLVFGLLC
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LMLALVVFAVAPTGLVFGLALPIYAAGAVAAPALMAILAGRVEREQQGRLQGASVAVQGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ASAVGPLLFGAAYALNSQNGVPHRGAGAMLLGAALLAVAATIAHAAARVPPRMPRARSEP
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PPLTGARGASWRPGFPHPVFVVAALDAMSVGIIVPALPHLVEQLAGSDGADPAVWLALL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AAAWGAAQFLSAPIMGVLADRFGRRPVLLAALAGLACDALFMALAPSLGWLLVGRLISGA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAASIIACSAYIIDVSAGDELARRLGLLGAAWSAGFIAGPALGGALADLHLRLPFLVCGA
HHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
SAAVAWLYARFRLKESLPIERRSTGLVWRRAVPFGGLTALLRRRALRTHGLVLALAALAD
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QALPTFFVLYVLERYGWSASAAGLALMGLGAARVLAQAVAVAPLTRRYGEPATLVFGLLC
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LMLALVVFAVAPTGLVFGLALPIYAAGAVAAPALMAILAGRVEREQQGRLQGASVAVQGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ASAVGPLLFGAAYALNSQNGVPHRGAGAMLLGAALLAVAATIAHAAARVPPRMPRARSEP
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312499; 6310527 [H]