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The map label for this gene is glyS

Identifier: 197104831

GI number: 197104831

Start: 1546416

End: 1548425

Strand: Direct

Name: glyS

Synonym: PHZ_c1365

Alternate gene names: 197104831

Gene position: 1546416-1548425 (Clockwise)

Preceding gene: 197104830

Following gene: 197104832

Centisome position: 38.7

GC content: 71.19

Gene sequence:

>2010_bases
ATGCCCCAACTCCTGCTCGAACTGCTCTCGGAAGAGATTCCGGCCCGCATGCAGGCGCAGGCCGCGCGCGACCTGGAGCG
CCTGGCGCGCGAAAGGCTCGCGGCCGAGGGGCTGCTGCCCGAGGCGCTGAAGACCTTCGCCGGCCCGCGGCGCCTGACGC
TGGTGGCCGAGGGGCTGCCGGCCGCCCAGGCCGACCGCCGCGAGGAGCTCAAGGGCCCCAAAGTCGGCGCTCCGGAACAG
GCGTTGGAGGGCTTCCTGCGCAAGACCGGGCTGACCCGCGACCAGCTGGTCGAGCGGGACGGCGTGTTCTTCGCGACGAT
CGAAAAGCCGGGCCGGCCGACGCCGGAGATCGTCGCCGAGATGGTCGAGGCCATCCTGCGGACCTTCCCCTGGCCTAAGT
CGATGGTTTCGGGGACCAGCAAGCTGCGCTGGGTGCGCCCGCTGCGGCGCATCCTCTGCGTCTTCGACGGCGAGGTCGTG
CCCTTCGAAGTGGACGGGATCGCGAGCGGCGACCTGTCGGAAGGCCACCGCTTCATGAGCGACGGCCAGCCGTTCCTGGT
GAAGGATTTCGAGGGCTACGCCGCGGGGCTCTCGCACCGCTCGGTGGTGCTCGACGCCGACGAGCGGAAGGAACGCATCC
TCGAGGCGGCCAAGACGCTGTGCTTCGCGCGCAACCTCGAGCTGGTCGAGGACGCCGGCCTGCTGGACGAGGTCGCGGGC
CTGGTCGAATGGCCGGTCCCGGTGCTGGGCGACATGGACCCCGCGTTCCTGGACCTGCCGCCCGAGGTGATCCGCACCTC
GATGCGGGTGCACCAGCGCTATTTCGCCGTGCGCGACCCGGCCGGCGGCAAGCTCGCGCCGCACTTCCTGACGGTGGCCA
ACATCGCGGCCCGCGACGGCGGGGCGACCATCGCCAAGGGCAACGCCAAGGTGCTCTCGGCCCGCCTGTCGGACGCGCGG
TTCTTCTGGGACGAGGACCGCAAGGTGCGCCTGGAGGACCGGCTGGAGAAGCTGAAGGGCGTCACCTTCCACGCCAAGCT
CGGGACCATGTACGAGCGGGTGCAGCGCATCGAGGCTCTGGCCGGCGAGCTGGCCCCGTTCGTGCGCGACGAGCCGGAAA
CCAGGACCAAGGCCGTGCAGGCCGCGCGGCTGGCCAAGGCCGACCTCGTCTCGGGCGTGGTGGGCGAATTCCCCGAGCTG
CAGGGGATCATGGGCGGCTACTACGCCGAGGCCGAGGGGCTGGACCCCGAGGTCGTCGACGCGATCCGCAGCCACTATCG
CCCGCAGGGGCCGAACGACGCCGTGCCGGTCAGCTCGGTGGCCGCCACCGTGGCCCTGGCCGACAAGCTGGACACGCTCG
TCAGCTTCTTCGGCATCGGCGAGAAGCCGACCGGCTCGCGCGATCCCTTCGCCCTGCGCCGCGCCGCGCTGGGCGTGATC
CGCATCGTGCTGGAAACCCGTACCCGCCTGCCGCTGAAGCGGTTCGTCTCGGACGAGGTGCTGGACTTCTTCGCTGACCG
CCTGGCCGTCCTGCTGCGCGAGCAGGGCAAGCGGCACGACCTGGTGGCGGCGGTGTTCGCCCTGGGGGACGACGACCTGG
TGCGTATCGTGGCCCGGGTCGAGGTCCTCTCGGCCTTCCTGAAGACCGAGGACGGGGCCAACCTGCTGGCCGGCTACAAG
CGGGCGGTGAACATCCTGCGGGCCGAGGAGAAGAAGGGCCCGCTGCCCGCCGGCGAGCCCGCGCAGGCGGCCGGGGCGCC
GGCGGAGGAAGCTGCGCTGGTGCAGGCCGTGGCCGCCCTGGACGCCCGACTGGGGCCCGCTCTGGAGCGCGAGGACTTCG
AAGGCGCCATGACCGAGCTCGCCAAGCTGCGCGGGCCGGTGGACGCGTTCTTCGACAAGGTGCTTGTGAACTCGGACGTT
CCGGCGGAACGGGAGAACAGGTTGCGCCTTCTTGCCAAGGTTCGCGACGCCATGGGGCGGGTTGCGGACTTCTCGCAGGT
CACAGGTTGA

Upstream 100 bases:

>100_bases
GCCGTCGCCGAGCGGCAGAGCTACATCGGCCGCATCCGCGACCTCTGCAAACTGTGCGCCGAGGCCTGGGTCGAGAACGA
AGGGGCGAACGCGTAAGGCC

Downstream 100 bases:

>100_bases
TTTCGCCCGCGCTGAGCGGGCCGGGTGGAGGTGAAGAGGCTATGGCCGACACGATGGTGAAGACGCGGTGGGTCTACGCT
TTCGGCGGCGGCGGGGCGGA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MPQLLLELLSEEIPARMQAQAARDLERLARERLAAEGLLPEALKTFAGPRRLTLVAEGLPAAQADRREELKGPKVGAPEQ
ALEGFLRKTGLTRDQLVERDGVFFATIEKPGRPTPEIVAEMVEAILRTFPWPKSMVSGTSKLRWVRPLRRILCVFDGEVV
PFEVDGIASGDLSEGHRFMSDGQPFLVKDFEGYAAGLSHRSVVLDADERKERILEAAKTLCFARNLELVEDAGLLDEVAG
LVEWPVPVLGDMDPAFLDLPPEVIRTSMRVHQRYFAVRDPAGGKLAPHFLTVANIAARDGGATIAKGNAKVLSARLSDAR
FFWDEDRKVRLEDRLEKLKGVTFHAKLGTMYERVQRIEALAGELAPFVRDEPETRTKAVQAARLAKADLVSGVVGEFPEL
QGIMGGYYAEAEGLDPEVVDAIRSHYRPQGPNDAVPVSSVAATVALADKLDTLVSFFGIGEKPTGSRDPFALRRAALGVI
RIVLETRTRLPLKRFVSDEVLDFFADRLAVLLREQGKRHDLVAAVFALGDDDLVRIVARVEVLSAFLKTEDGANLLAGYK
RAVNILRAEEKKGPLPAGEPAQAAGAPAEEAALVQAVAALDARLGPALEREDFEGAMTELAKLRGPVDAFFDKVLVNSDV
PAERENRLRLLAKVRDAMGRVADFSQVTG

Sequences:

>Translated_669_residues
MPQLLLELLSEEIPARMQAQAARDLERLARERLAAEGLLPEALKTFAGPRRLTLVAEGLPAAQADRREELKGPKVGAPEQ
ALEGFLRKTGLTRDQLVERDGVFFATIEKPGRPTPEIVAEMVEAILRTFPWPKSMVSGTSKLRWVRPLRRILCVFDGEVV
PFEVDGIASGDLSEGHRFMSDGQPFLVKDFEGYAAGLSHRSVVLDADERKERILEAAKTLCFARNLELVEDAGLLDEVAG
LVEWPVPVLGDMDPAFLDLPPEVIRTSMRVHQRYFAVRDPAGGKLAPHFLTVANIAARDGGATIAKGNAKVLSARLSDAR
FFWDEDRKVRLEDRLEKLKGVTFHAKLGTMYERVQRIEALAGELAPFVRDEPETRTKAVQAARLAKADLVSGVVGEFPEL
QGIMGGYYAEAEGLDPEVVDAIRSHYRPQGPNDAVPVSSVAATVALADKLDTLVSFFGIGEKPTGSRDPFALRRAALGVI
RIVLETRTRLPLKRFVSDEVLDFFADRLAVLLREQGKRHDLVAAVFALGDDDLVRIVARVEVLSAFLKTEDGANLLAGYK
RAVNILRAEEKKGPLPAGEPAQAAGAPAEEAALVQAVAALDARLGPALEREDFEGAMTELAKLRGPVDAFFDKVLVNSDV
PAERENRLRLLAKVRDAMGRVADFSQVTG
>Mature_668_residues
PQLLLELLSEEIPARMQAQAARDLERLARERLAAEGLLPEALKTFAGPRRLTLVAEGLPAAQADRREELKGPKVGAPEQA
LEGFLRKTGLTRDQLVERDGVFFATIEKPGRPTPEIVAEMVEAILRTFPWPKSMVSGTSKLRWVRPLRRILCVFDGEVVP
FEVDGIASGDLSEGHRFMSDGQPFLVKDFEGYAAGLSHRSVVLDADERKERILEAAKTLCFARNLELVEDAGLLDEVAGL
VEWPVPVLGDMDPAFLDLPPEVIRTSMRVHQRYFAVRDPAGGKLAPHFLTVANIAARDGGATIAKGNAKVLSARLSDARF
FWDEDRKVRLEDRLEKLKGVTFHAKLGTMYERVQRIEALAGELAPFVRDEPETRTKAVQAARLAKADLVSGVVGEFPELQ
GIMGGYYAEAEGLDPEVVDAIRSHYRPQGPNDAVPVSSVAATVALADKLDTLVSFFGIGEKPTGSRDPFALRRAALGVIR
IVLETRTRLPLKRFVSDEVLDFFADRLAVLLREQGKRHDLVAAVFALGDDDLVRIVARVEVLSAFLKTEDGANLLAGYKR
AVNILRAEEKKGPLPAGEPAQAAGAPAEEAALVQAVAALDARLGPALEREDFEGAMTELAKLRGPVDAFFDKVLVNSDVP
AERENRLRLLAKVRDAMGRVADFSQVTG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=695, Percent_Identity=36.2589928057554, Blast_Score=355, Evalue=4e-99,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_PHEZH (B4R9A9)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130208.1
- GeneID:   6790871
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1365
- HOGENOM:   HBG586599
- OMA:   SFERDFL
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 73166; Mature: 73035

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQLLLELLSEEIPARMQAQAARDLERLARERLAAEGLLPEALKTFAGPRRLTLVAEGLP
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCC
AAQADRREELKGPKVGAPEQALEGFLRKTGLTRDQLVERDGVFFATIEKPGRPTPEIVAE
CHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHH
MVEAILRTFPWPKSMVSGTSKLRWVRPLRRILCVFDGEVVPFEVDGIASGDLSEGHRFMS
HHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHCCHHHC
DGQPFLVKDFEGYAAGLSHRSVVLDADERKERILEAAKTLCFARNLELVEDAGLLDEVAG
CCCCEEEECCCHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHH
LVEWPVPVLGDMDPAFLDLPPEVIRTSMRVHQRYFAVRDPAGGKLAPHFLTVANIAARDG
HHHCCCHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCC
GATIAKGNAKVLSARLSDARFFWDEDRKVRLEDRLEKLKGVTFHAKLGTMYERVQRIEAL
CCEEECCCHHHHHHHHHCCCEEECCCCEECHHHHHHHHHCCEEEHHHHHHHHHHHHHHHH
AGELAPFVRDEPETRTKAVQAARLAKADLVSGVVGEFPELQGIMGGYYAEAEGLDPEVVD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHCCCCCCCHHHHH
AIRSHYRPQGPNDAVPVSSVAATVALADKLDTLVSFFGIGEKPTGSRDPFALRRAALGVI
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
RIVLETRTRLPLKRFVSDEVLDFFADRLAVLLREQGKRHDLVAAVFALGDDDLVRIVARV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHH
EVLSAFLKTEDGANLLAGYKRAVNILRAEEKKGPLPAGEPAQAAGAPAEEAALVQAVAAL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHH
DARLGPALEREDFEGAMTELAKLRGPVDAFFDKVLVNSDVPAERENRLRLLAKVRDAMGR
HHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VADFSQVTG
HHHHHHCCC
>Mature Secondary Structure 
PQLLLELLSEEIPARMQAQAARDLERLARERLAAEGLLPEALKTFAGPRRLTLVAEGLP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCC
AAQADRREELKGPKVGAPEQALEGFLRKTGLTRDQLVERDGVFFATIEKPGRPTPEIVAE
CHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHH
MVEAILRTFPWPKSMVSGTSKLRWVRPLRRILCVFDGEVVPFEVDGIASGDLSEGHRFMS
HHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHCCHHHC
DGQPFLVKDFEGYAAGLSHRSVVLDADERKERILEAAKTLCFARNLELVEDAGLLDEVAG
CCCCEEEECCCHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHH
LVEWPVPVLGDMDPAFLDLPPEVIRTSMRVHQRYFAVRDPAGGKLAPHFLTVANIAARDG
HHHCCCHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCC
GATIAKGNAKVLSARLSDARFFWDEDRKVRLEDRLEKLKGVTFHAKLGTMYERVQRIEAL
CCEEECCCHHHHHHHHHCCCEEECCCCEECHHHHHHHHHCCEEEHHHHHHHHHHHHHHHH
AGELAPFVRDEPETRTKAVQAARLAKADLVSGVVGEFPELQGIMGGYYAEAEGLDPEVVD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHCCCCCCCHHHHH
AIRSHYRPQGPNDAVPVSSVAATVALADKLDTLVSFFGIGEKPTGSRDPFALRRAALGVI
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
RIVLETRTRLPLKRFVSDEVLDFFADRLAVLLREQGKRHDLVAAVFALGDDDLVRIVARV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHH
EVLSAFLKTEDGANLLAGYKRAVNILRAEEKKGPLPAGEPAQAAGAPAEEAALVQAVAAL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHH
DARLGPALEREDFEGAMTELAKLRGPVDAFFDKVLVNSDVPAERENRLRLLAKVRDAMGR
HHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VADFSQVTG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA