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The map label for this gene is ispE [H]

Identifier: 197104825

GI number: 197104825

Start: 1541105

End: 1542028

Strand: Direct

Name: ispE [H]

Synonym: PHZ_c1359

Alternate gene names: 197104825

Gene position: 1541105-1542028 (Clockwise)

Preceding gene: 197104824

Following gene: 197104828

Centisome position: 38.56

GC content: 77.06

Gene sequence:

>924_bases
TTGGGCCGCAGGCGCCGAAGCTCGCCGGACAGTAGGGTGCCCGCCGTCTTCGCGCCGGCCAAGGTCAACCTCTTCCTGCA
CGTGGGCGCGCCGGAGCCCTCGGGCTACCACCCGATCTGCAGCCTGATGACGTTCGCCGACGTGGGCGACCGGGTCAGCG
CGGCCGGGGCCGACGCCCTGGATCTCGTGGTGGACGGGCCGTTCGCCCGCGAGCTGGCGGGGGAGGGCGACAACCTCGTC
CTGCGCGCCGCTCGCGCGCTGATCGCCGAGGCGCGCCGGCCGGTTCCACCGATCGCGCTGCGCCTGGAGAAGCGCCTGCC
GGTGGCCAGCGGCCTGGGCGGCGGCTCCAGCGACGCCGGCGCGGCCTTGCGGCTGATGCGCGAGGCGCTGGCGCTCGACG
TTTCCGACGCGCGGCTGGAAGCCGTGGCAGCTAGCCTCGGGGCGGACGGCGCCGCCTGCCTCTGGGGACGTCCCGCACTC
GCCCAGGGGCGGGGCGAGCGTCTTTCGCCCGCGCCGGCCATGCCGGTCCTCGACGCGGTGCTGGTCAATCCGGGCGCGCC
CGTCTCCACTGCCGACGTCTACCGGCGCTTCGACGCGGCCGGCGCCTTCGGGTCCGTGGAGCCGCCCGCAGCGCCCGAAG
CGTTCGAGAGTCCCCAGGAGGTCGCCGCCTGGCTGGCCGGCCAGCGCAACGATCTGGAGGCCCCGGCGCTCGCCGTCGCA
CCGGCGGTCGGCGCGGCGCTGGAGACCCTGCGCGCCGAGCCCGAGACCCTGCTGGCGCGCGTGTCCGGCTCGGGCGGCAC
CTGCTTCGCCCTGTGCGCCGGCGACATCGAGGCCGAGAGCCTGGCCGAGCGGCTGCAGGCCCTGGCGCCGGACTGGTGGG
TCGTCCGCTGCCGGCTCGGCGGCCCCTCGCCGGACGCGGCCTGA

Upstream 100 bases:

>100_bases
GGGCGCAAGGACGAGGCGGTCTTCCAATGGCGGCGCGTCCTGACGCTGAAGCCGGACGCCAAGATCCAGGCCGACGCCGA
GGCCAAGCTGGCCTCGGGCC

Downstream 100 bases:

>100_bases
CCGCCCAAACGGAAGCGGGGGCCCCGAAGGACCCCCGCGCCACCCCGAAGACGCCGCCGCGAGGCGACGCCTCTCCCCCG
GAGCGGATCAGGTCTCGTGC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MGRRRRSSPDSRVPAVFAPAKVNLFLHVGAPEPSGYHPICSLMTFADVGDRVSAAGADALDLVVDGPFARELAGEGDNLV
LRAARALIAEARRPVPPIALRLEKRLPVASGLGGGSSDAGAALRLMREALALDVSDARLEAVAASLGADGAACLWGRPAL
AQGRGERLSPAPAMPVLDAVLVNPGAPVSTADVYRRFDAAGAFGSVEPPAAPEAFESPQEVAAWLAGQRNDLEAPALAVA
PAVGAALETLRAEPETLLARVSGSGGTCFALCAGDIEAESLAERLQALAPDWWVVRCRLGGPSPDAA

Sequences:

>Translated_307_residues
MGRRRRSSPDSRVPAVFAPAKVNLFLHVGAPEPSGYHPICSLMTFADVGDRVSAAGADALDLVVDGPFARELAGEGDNLV
LRAARALIAEARRPVPPIALRLEKRLPVASGLGGGSSDAGAALRLMREALALDVSDARLEAVAASLGADGAACLWGRPAL
AQGRGERLSPAPAMPVLDAVLVNPGAPVSTADVYRRFDAAGAFGSVEPPAAPEAFESPQEVAAWLAGQRNDLEAPALAVA
PAVGAALETLRAEPETLLARVSGSGGTCFALCAGDIEAESLAERLQALAPDWWVVRCRLGGPSPDAA
>Mature_306_residues
GRRRRSSPDSRVPAVFAPAKVNLFLHVGAPEPSGYHPICSLMTFADVGDRVSAAGADALDLVVDGPFARELAGEGDNLVL
RAARALIAEARRPVPPIALRLEKRLPVASGLGGGSSDAGAALRLMREALALDVSDARLEAVAASLGADGAACLWGRPALA
QGRGERLSPAPAMPVLDAVLVNPGAPVSTADVYRRFDAAGAFGSVEPPAAPEAFESPQEVAAWLAGQRNDLEAPALAVAP
AVGAALETLRAEPETLLARVSGSGGTCFALCAGDIEAESLAERLQALAPDWWVVRCRLGGPSPDAA

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=282, Percent_Identity=30.8510638297872, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 31468; Mature: 31337

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRRRRSSPDSRVPAVFAPAKVNLFLHVGAPEPSGYHPICSLMTFADVGDRVSAAGADAL
CCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHCCCCEE
DLVVDGPFARELAGEGDNLVLRAARALIAEARRPVPPIALRLEKRLPVASGLGGGSSDAG
EEEECCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCCCCCCHHH
AALRLMREALALDVSDARLEAVAASLGADGAACLWGRPALAQGRGERLSPAPAMPVLDAV
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCCCHHHCCCCCCCCCCCCCHHHHHH
LVNPGAPVSTADVYRRFDAAGAFGSVEPPAAPEAFESPQEVAAWLAGQRNDLEAPALAVA
HCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCCCCCCHHHHH
PAVGAALETLRAEPETLLARVSGSGGTCFALCAGDIEAESLAERLQALAPDWWVVRCRLG
HHHHHHHHHHHCCHHHHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECC
GPSPDAA
CCCCCCC
>Mature Secondary Structure 
GRRRRSSPDSRVPAVFAPAKVNLFLHVGAPEPSGYHPICSLMTFADVGDRVSAAGADAL
CCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHCCCCEE
DLVVDGPFARELAGEGDNLVLRAARALIAEARRPVPPIALRLEKRLPVASGLGGGSSDAG
EEEECCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCCCCCCHHH
AALRLMREALALDVSDARLEAVAASLGADGAACLWGRPALAQGRGERLSPAPAMPVLDAV
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCCCHHHCCCCCCCCCCCCCHHHHHH
LVNPGAPVSTADVYRRFDAAGAFGSVEPPAAPEAFESPQEVAAWLAGQRNDLEAPALAVA
HCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCCCCCCHHHHH
PAVGAALETLRAEPETLLARVSGSGGTCFALCAGDIEAESLAERLQALAPDWWVVRCRLG
HHHHHHHHHHHCCHHHHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECC
GPSPDAA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]