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The map label for this gene is pleD [H]

Identifier: 197104776

GI number: 197104776

Start: 1487674

End: 1489038

Strand: Direct

Name: pleD [H]

Synonym: PHZ_c1310

Alternate gene names: 197104776

Gene position: 1487674-1489038 (Clockwise)

Preceding gene: 197104775

Following gene: 197104789

Centisome position: 37.23

GC content: 71.58

Gene sequence:

>1365_bases
ATGTCGGCCCGCATCCTGGTGGTGGACGACATCGAGGCGAACGTCCGGCTGCTCGAGGCCAAGCTGACGGCCGAGTACTA
CGAGGTGCTGACCGCCTCCGACGGCCCGACGGCCCTGGCCATGGCCGCGGCCGAACGGCCCGACATAGTGCTGCTGGACG
TGATGATGCCCGGCATGGACGGCTTCTCGGTCTGCCGCAGGCTCAAGGACGATCCCGAGACGCGCCACGTGCCGGTGGTG
CTGGTCACCGCGCTGGACGGCCGCGCCGACCGCGTGGCGGGCCTCGAGGCGGGCGCCGACGATTTCCTGACCAAGCCCAT
CGACGACGTCATGCTGCTCGCCCGCGTTCGCAGCCTGACCCGCCTGAAGGCGGTGATCGACGAGCTGCGCGAGCGCGAGG
CCTCTGGCCGCCGGATGGGCGTGATCGCCGGCGCGGCCTCGCGCCTGGGCGGCTCGGGCGGGCGGATCCTGATCATCGAC
GACAACGTCCGCCAGGCGCAGCGGGTCTGCACCGAACTGGCCATAGAGCACCGGCCGGTGATCGAGACCGACTACGACAA
GGCCCTGCTCAGCGCCCGGGGCCCCGTGGACCTGGCGATCGTCAATGCGGGCGCGAAGGCCTTCGACGGCCTGCGGTTCT
GCGCCCAGCTGCGCTCGGACGAGGCCACGCGCGGCCTGCCGATCCTGGCGGTGGTGGACTTCGACGAGCGCCAGCGGGTC
GTGAAGGCGCTGGAGATCGGGGTGAACGACATCCTGGCCCGGCCGATCGATCCGGGCGAGCTCGCCGCCCGCGCCAAGAC
CCAGATCCGCCGCAAGCGCTACACCGAGTACCTGCGCAACAACCTGGACCATTCGCTGGAGCTGGCGGTCACCGACCAGC
TCACAGGCCTGCACAACCGCCGCTACATGCAGGGCCAGCTCGAGGCGCTGATGCGCCGGGCGGCGGCGGGCGGGGATCCC
GTGGCCCTGCTGGTCATCGACATCGACCATTTCAAGCGGATCAACGACAGCTTCGGCCACGACGTGGGCGACGAGGTGCT
GCGCGAGTTCGCCGTCCGCCTGGCCTCGAACGTGCGGGCGATCGACCTGCCGGTGCGCCACGGCGGCGAGGAATTCGTCG
TCGTCATGCCCGACACCAGCCTGGAGGACGCGCGCCGCATCGCCGAGCGCATCCGCCTGCACGTGGCCGGCTCGCCGTTC
CGCGTGATGGGCGGCGAGGAGCTGCTGTCGGTGACGATCTCCATCGGCGTGGCGGCGGGCGCGGGCGGGGAGGACAGCTC
GCAGGCGCTGCTCAAGCGCGCCGACGAGGCGGTCTACGAGGCCAAGTCCCGCGGCCGCAACCGGGTCATCGCCCGGGCGG
CCTGA

Upstream 100 bases:

>100_bases
AGGAGCGGATCCGCGAGGGCGGCTGCGAGGCCTACATCTCCAAGCCCATCTCCGTCTCGCACTTCCTCGAAACCATCCGC
CGCCTCCTGGACTAGCGCCG

Downstream 100 bases:

>100_bases
TCTTTCCGCAACGAAAAACGCCCGGCGGTGAGGCCGGGCGTCTTCAACGTAATCAGCGGCTTGGCTGGATTACTTGATCT
TGCCTTCCTTGAACTCGACG

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSARILVVDDIEANVRLLEAKLTAEYYEVLTASDGPTALAMAAAERPDIVLLDVMMPGMDGFSVCRRLKDDPETRHVPVV
LVTALDGRADRVAGLEAGADDFLTKPIDDVMLLARVRSLTRLKAVIDELREREASGRRMGVIAGAASRLGGSGGRILIID
DNVRQAQRVCTELAIEHRPVIETDYDKALLSARGPVDLAIVNAGAKAFDGLRFCAQLRSDEATRGLPILAVVDFDERQRV
VKALEIGVNDILARPIDPGELAARAKTQIRRKRYTEYLRNNLDHSLELAVTDQLTGLHNRRYMQGQLEALMRRAAAGGDP
VALLVIDIDHFKRINDSFGHDVGDEVLREFAVRLASNVRAIDLPVRHGGEEFVVVMPDTSLEDARRIAERIRLHVAGSPF
RVMGGEELLSVTISIGVAAGAGGEDSSQALLKRADEAVYEAKSRGRNRVIARAA

Sequences:

>Translated_454_residues
MSARILVVDDIEANVRLLEAKLTAEYYEVLTASDGPTALAMAAAERPDIVLLDVMMPGMDGFSVCRRLKDDPETRHVPVV
LVTALDGRADRVAGLEAGADDFLTKPIDDVMLLARVRSLTRLKAVIDELREREASGRRMGVIAGAASRLGGSGGRILIID
DNVRQAQRVCTELAIEHRPVIETDYDKALLSARGPVDLAIVNAGAKAFDGLRFCAQLRSDEATRGLPILAVVDFDERQRV
VKALEIGVNDILARPIDPGELAARAKTQIRRKRYTEYLRNNLDHSLELAVTDQLTGLHNRRYMQGQLEALMRRAAAGGDP
VALLVIDIDHFKRINDSFGHDVGDEVLREFAVRLASNVRAIDLPVRHGGEEFVVVMPDTSLEDARRIAERIRLHVAGSPF
RVMGGEELLSVTISIGVAAGAGGEDSSQALLKRADEAVYEAKSRGRNRVIARAA
>Mature_453_residues
SARILVVDDIEANVRLLEAKLTAEYYEVLTASDGPTALAMAAAERPDIVLLDVMMPGMDGFSVCRRLKDDPETRHVPVVL
VTALDGRADRVAGLEAGADDFLTKPIDDVMLLARVRSLTRLKAVIDELREREASGRRMGVIAGAASRLGGSGGRILIIDD
NVRQAQRVCTELAIEHRPVIETDYDKALLSARGPVDLAIVNAGAKAFDGLRFCAQLRSDEATRGLPILAVVDFDERQRVV
KALEIGVNDILARPIDPGELAARAKTQIRRKRYTEYLRNNLDHSLELAVTDQLTGLHNRRYMQGQLEALMRRAAAGGDPV
ALLVIDIDHFKRINDSFGHDVGDEVLREFAVRLASNVRAIDLPVRHGGEEFVVVMPDTSLEDARRIAERIRLHVAGSPFR
VMGGEELLSVTISIGVAAGAGGEDSSQALLKRADEAVYEAKSRGRNRVIARAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=41.7721518987342, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=44.8275862068966, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=38.1818181818182, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=37.7906976744186, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=37.2670807453416, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=36.6459627329193, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788085, Length=172, Percent_Identity=37.2093023255814, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=36.4779874213836, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=35.5421686746988, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788381, Length=161, Percent_Identity=37.2670807453416, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=38.2113821138211, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=40.1639344262295, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI87082012, Length=145, Percent_Identity=34.4827586206897, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788394, Length=135, Percent_Identity=35.5555555555556, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081974, Length=162, Percent_Identity=30.8641975308642, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788956, Length=166, Percent_Identity=34.3373493975904, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=36.4406779661017, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=143, Percent_Identity=35.6643356643357, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI2367329, Length=118, Percent_Identity=41.5254237288136, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790863, Length=118, Percent_Identity=32.2033898305085, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081977, Length=167, Percent_Identity=31.7365269461078, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787229, Length=119, Percent_Identity=33.6134453781513, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=208, Percent_Identity=30.2884615384615, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786911, Length=113, Percent_Identity=35.3982300884956, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=41.747572815534, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790860, Length=119, Percent_Identity=35.2941176470588, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789149, Length=111, Percent_Identity=32.4324324324324, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI145693140, Length=105, Percent_Identity=36.1904761904762, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 49554; Mature: 49422

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARILVVDDIEANVRLLEAKLTAEYYEVLTASDGPTALAMAAAERPDIVLLDVMMPGMD
CCCEEEEEECCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEHHCCCCCEEEEEEECCCCC
GFSVCRRLKDDPETRHVPVVLVTALDGRADRVAGLEAGADDFLTKPIDDVMLLARVRSLT
HHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHH
RLKAVIDELREREASGRRMGVIAGAASRLGGSGGRILIIDDNVRQAQRVCTELAIEHRPV
HHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCC
IETDYDKALLSARGPVDLAIVNAGAKAFDGLRFCAQLRSDEATRGLPILAVVDFDERQRV
CCCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHH
VKALEIGVNDILARPIDPGELAARAKTQIRRKRYTEYLRNNLDHSLELAVTDQLTGLHNR
HHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHCCCCC
RYMQGQLEALMRRAAAGGDPVALLVIDIDHFKRINDSFGHDVGDEVLREFAVRLASNVRA
HHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEE
IDLPVRHGGEEFVVVMPDTSLEDARRIAERIRLHVAGSPFRVMGGEELLSVTISIGVAAG
EEEEHCCCCCEEEEEECCCCHHHHHHHHHHHEEEECCCCEEEECCHHHEEEEEEEEEEEC
AGGEDSSQALLKRADEAVYEAKSRGRNRVIARAA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
SARILVVDDIEANVRLLEAKLTAEYYEVLTASDGPTALAMAAAERPDIVLLDVMMPGMD
CCEEEEEECCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEHHCCCCCEEEEEEECCCCC
GFSVCRRLKDDPETRHVPVVLVTALDGRADRVAGLEAGADDFLTKPIDDVMLLARVRSLT
HHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHH
RLKAVIDELREREASGRRMGVIAGAASRLGGSGGRILIIDDNVRQAQRVCTELAIEHRPV
HHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCC
IETDYDKALLSARGPVDLAIVNAGAKAFDGLRFCAQLRSDEATRGLPILAVVDFDERQRV
CCCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHH
VKALEIGVNDILARPIDPGELAARAKTQIRRKRYTEYLRNNLDHSLELAVTDQLTGLHNR
HHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHCCCCC
RYMQGQLEALMRRAAAGGDPVALLVIDIDHFKRINDSFGHDVGDEVLREFAVRLASNVRA
HHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEE
IDLPVRHGGEEFVVVMPDTSLEDARRIAERIRLHVAGSPFRVMGGEELLSVTISIGVAAG
EEEEHCCCCCEEEEEECCCCHHHHHHHHHHHEEEECCCCEEEECCHHHEEEEEEEEEEEC
AGGEDSSQALLKRADEAVYEAKSRGRNRVIARAA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA