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The map label for this gene is menB [C]

Identifier: 197104755

GI number: 197104755

Start: 1466661

End: 1467440

Strand: Direct

Name: menB [C]

Synonym: PHZ_c1289

Alternate gene names: 197104755

Gene position: 1466661-1467440 (Clockwise)

Preceding gene: 197104751

Following gene: 197104756

Centisome position: 36.7

GC content: 72.69

Gene sequence:

>780_bases
GTGCGGCTGGACTCCACGGCCGAGGGCGTGGTGGTCGTCACCCTCAACCGGCCCCAGCGGAAGAACGCCTTCGACGCCGA
CCTGATCTCGGCGATGGAGGAGGCCTTTTCGACCCTGCACGCGCAGGAGCACGTGCGGGTGGTGTTCGTGCGTGGCGCGG
GCGGGATGTTCAGCGCCGGCGCCGACCTGGAGTGGATGCGCGCCGCCGCCGACCGCTCCGAGGCCGACAACCGCGACGAC
GCCTTCCAGATGGCCAAGATGCTGAAGGCGCTGTGGGACATCCCGGCGCTGACGGTGGCCCTGGTGGAGGGCGGGGCGTT
CGGCGGCGGCGCGGGCGTCGCGGCCGCGTGCGACCTGGCGATCGCCACGGCGGACGCGAAGTTCAGCTTCTCCGAGGTCC
GGCTGGGCCTGGTGCCGGCCACCATCAGTCCGTACGTCGTCCAGGCGATCGGCCCGCGCGCCGCCCGCGGCCTGTTCGCC
ACGGCGCGCACCTTCGACGCCGCCTACGCCGAGAAGATCGGCCTCGTCACCGAGGTGGTGGCCGACGCGGCCGAGCTGAA
CAAGGCCATGGACCGGGTGATCGACCACATCCTCTCGTGCGCGCCGGGCGCCGTGGCCGACTCCAAGCGGCTGGTGCACG
ACGTCTACGGCCACCGGATCGATCACGGCCTGATGGACATGACCGCCCACCGCATCGCCGCCGCCCGCGTCGGCGAGGAA
GGGCAGGAGGGCGTCCGCGCCTTCCTCGAGCGGCGCAAGCCGTCCTGGGCCCAAGGCTGA

Upstream 100 bases:

>100_bases
GGGGGCTATCAGCTTGCCGGAAGAGGAGTGAGACCGAATGCCCAATCCCATCGCCGACCCGCTCGTCGAAGGCGTCGACG
TCAGCGCCACCTCCGGCCTC

Downstream 100 bases:

>100_bases
CGGACGCGGCGGCCTGAGGCGGGCCGCCGCACTTTCCAAGGACCGCGCCGGGCCGTAATCCCAGGGCATGGCGCTGCACA
TGATCAAGCTGGTGGTCGGC

Product: enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MRLDSTAEGVVVVTLNRPQRKNAFDADLISAMEEAFSTLHAQEHVRVVFVRGAGGMFSAGADLEWMRAAADRSEADNRDD
AFQMAKMLKALWDIPALTVALVEGGAFGGGAGVAAACDLAIATADAKFSFSEVRLGLVPATISPYVVQAIGPRAARGLFA
TARTFDAAYAEKIGLVTEVVADAAELNKAMDRVIDHILSCAPGAVADSKRLVHDVYGHRIDHGLMDMTAHRIAAARVGEE
GQEGVRAFLERRKPSWAQG

Sequences:

>Translated_259_residues
MRLDSTAEGVVVVTLNRPQRKNAFDADLISAMEEAFSTLHAQEHVRVVFVRGAGGMFSAGADLEWMRAAADRSEADNRDD
AFQMAKMLKALWDIPALTVALVEGGAFGGGAGVAAACDLAIATADAKFSFSEVRLGLVPATISPYVVQAIGPRAARGLFA
TARTFDAAYAEKIGLVTEVVADAAELNKAMDRVIDHILSCAPGAVADSKRLVHDVYGHRIDHGLMDMTAHRIAAARVGEE
GQEGVRAFLERRKPSWAQG
>Mature_259_residues
MRLDSTAEGVVVVTLNRPQRKNAFDADLISAMEEAFSTLHAQEHVRVVFVRGAGGMFSAGADLEWMRAAADRSEADNRDD
AFQMAKMLKALWDIPALTVALVEGGAFGGGAGVAAACDLAIATADAKFSFSEVRLGLVPATISPYVVQAIGPRAARGLFA
TARTFDAAYAEKIGLVTEVVADAAELNKAMDRVIDHILSCAPGAVADSKRLVHDVYGHRIDHGLMDMTAHRIAAARVGEE
GQEGVRAFLERRKPSWAQG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI213417737, Length=184, Percent_Identity=30.9782608695652, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI157694516, Length=184, Percent_Identity=30.9782608695652, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=24.2063492063492, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI70995211, Length=220, Percent_Identity=30.4545454545455, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4502327, Length=268, Percent_Identity=27.6119402985075, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=26.5873015873016, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157694520, Length=139, Percent_Identity=32.3741007194245, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI20127408, Length=228, Percent_Identity=28.9473684210526, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=27.5862068965517, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI221142681, Length=257, Percent_Identity=29.9610894941634, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787659, Length=254, Percent_Identity=27.1653543307087, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=28.7878787878788, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787660, Length=265, Percent_Identity=26.4150943396226, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788682, Length=196, Percent_Identity=29.0816326530612, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790281, Length=182, Percent_Identity=28.021978021978, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=26.8482490272374, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=26.9230769230769, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=214, Percent_Identity=29.4392523364486, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=24.7058823529412, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=231, Percent_Identity=26.4069264069264, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=29.5454545454545, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=27.0161290322581, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=27.0161290322581, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=24.7104247104247, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24650670, Length=187, Percent_Identity=30.4812834224599, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19920382, Length=259, Percent_Identity=27.027027027027, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27578; Mature: 27578

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDSTAEGVVVVTLNRPQRKNAFDADLISAMEEAFSTLHAQEHVRVVFVRGAGGMFSAG
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCC
ADLEWMRAAADRSEADNRDDAFQMAKMLKALWDIPALTVALVEGGAFGGGAGVAAACDLA
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHH
IATADAKFSFSEVRLGLVPATISPYVVQAIGPRAARGLFATARTFDAAYAEKIGLVTEVV
HEECCCCCCHHHHHEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADAAELNKAMDRVIDHILSCAPGAVADSKRLVHDVYGHRIDHGLMDMTAHRIAAARVGEE
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GQEGVRAFLERRKPSWAQG
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRLDSTAEGVVVVTLNRPQRKNAFDADLISAMEEAFSTLHAQEHVRVVFVRGAGGMFSAG
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCC
ADLEWMRAAADRSEADNRDDAFQMAKMLKALWDIPALTVALVEGGAFGGGAGVAAACDLA
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHH
IATADAKFSFSEVRLGLVPATISPYVVQAIGPRAARGLFATARTFDAAYAEKIGLVTEVV
HEECCCCCCHHHHHEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADAAELNKAMDRVIDHILSCAPGAVADSKRLVHDVYGHRIDHGLMDMTAHRIAAARVGEE
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GQEGVRAFLERRKPSWAQG
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA