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The map label for this gene is lipB [H]

Identifier: 197104746

GI number: 197104746

Start: 1457036

End: 1457722

Strand: Reverse

Name: lipB [H]

Synonym: PHZ_c1280

Alternate gene names: 197104746

Gene position: 1457722-1457036 (Counterclockwise)

Preceding gene: 197104752

Following gene: 197104743

Centisome position: 36.48

GC content: 73.36

Gene sequence:

>687_bases
ATGTTCGGGCGGGACGACGACCGGCCGGCCGGCTGGGCCGTCTCGACGGGCCTCGTGGACTATCCCGCCGCCGTGGCGGC
GATGGAGGCCCGCGCCGCGGCCATCGCCGAGGGGAGCGCCGGCGAACTGGTGTGGCTGCTTGAGCATCCGCCGCTCTACA
CCGCGGGCGTCTCGGCCAAGCCCGCCGACCTGCTGGACGCCGGGCGATTCCCCGTCTTCGAGAGCGGCCGCGGCGGCCAA
TTCACCTACCACGGCCCGGGACAGCGGGTGGCCTACGTCATGCTGGACCTGACGAAGCGCGGGCGCGACGTGCGCGCCTT
CGTCGGCGCCCTGGAGGCCTGGGTGATCGGCGCGCTCGACGCCTTCAACGTCCGGGGCGAAATCCGCGAGGGCCGCGTAG
GCGTCTGGGTCGAGCGGCGCGCGCCGGGCGTCCCCCCGCGCGAGGACAAGATCGCCGCCATCGGCGTGAAGCTGCGCCGC
TGGGTGTCGTTCCACGGGATTGCGCTGAACGTGGAGCCGGACCTCTCGCATTTCTCCGGCATCGTGCCCTGCGGCGTGAC
CGAGCACGGGGTCACTAGCCTCGTGGACCTCGGCCTGCCGGTGACGTTGGACGAGGCCGACGCGGCCCTGAAGGCGAGCT
TCCGGCGGGTGTTCGGCGAGGTCGAGGACGCCGCCGCGCCGTTCTAG

Upstream 100 bases:

>100_bases
ACTCCACGCGAGGGGCACGAGGGGCTTTGCGCGAAGCGGCCCCAGCGCCCACATTGGCGGGAACATGCTTGACCACGCGT
TAAATTCGGCGGCCGCCCCC

Downstream 100 bases:

>100_bases
GCAGGCGCCAGCGGCCGCAGGGGATTGGGCCCGTAGCGGTTGGCGCCCGGCTCGCCCGGCATGACGGCCAGCTCGACGCC
GGCCCAGACCAGCACCAGGA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MFGRDDDRPAGWAVSTGLVDYPAAVAAMEARAAAIAEGSAGELVWLLEHPPLYTAGVSAKPADLLDAGRFPVFESGRGGQ
FTYHGPGQRVAYVMLDLTKRGRDVRAFVGALEAWVIGALDAFNVRGEIREGRVGVWVERRAPGVPPREDKIAAIGVKLRR
WVSFHGIALNVEPDLSHFSGIVPCGVTEHGVTSLVDLGLPVTLDEADAALKASFRRVFGEVEDAAAPF

Sequences:

>Translated_228_residues
MFGRDDDRPAGWAVSTGLVDYPAAVAAMEARAAAIAEGSAGELVWLLEHPPLYTAGVSAKPADLLDAGRFPVFESGRGGQ
FTYHGPGQRVAYVMLDLTKRGRDVRAFVGALEAWVIGALDAFNVRGEIREGRVGVWVERRAPGVPPREDKIAAIGVKLRR
WVSFHGIALNVEPDLSHFSGIVPCGVTEHGVTSLVDLGLPVTLDEADAALKASFRRVFGEVEDAAAPF
>Mature_228_residues
MFGRDDDRPAGWAVSTGLVDYPAAVAAMEARAAAIAEGSAGELVWLLEHPPLYTAGVSAKPADLLDAGRFPVFESGRGGQ
FTYHGPGQRVAYVMLDLTKRGRDVRAFVGALEAWVIGALDAFNVRGEIREGRVGVWVERRAPGVPPREDKIAAIGVKLRR
WVSFHGIALNVEPDLSHFSGIVPCGVTEHGVTSLVDLGLPVTLDEADAALKASFRRVFGEVEDAAAPF

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=190, Percent_Identity=35.2631578947368, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI87081767, Length=169, Percent_Identity=41.4201183431953, Blast_Score=124, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6323268, Length=179, Percent_Identity=28.4916201117318, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355483, Length=223, Percent_Identity=35.4260089686099, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 24359; Mature: 24359

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGRDDDRPAGWAVSTGLVDYPAAVAAMEARAAAIAEGSAGELVWLLEHPPLYTAGVSAK
CCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCC
PADLLDAGRFPVFESGRGGQFTYHGPGQRVAYVMLDLTKRGRDVRAFVGALEAWVIGALD
CHHHHCCCCCCEEECCCCCEEEEECCCCEEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHH
AFNVRGEIREGRVGVWVERRAPGVPPREDKIAAIGVKLRRWVSFHGIALNVEPDLSHFSG
HHCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHHEECCEEEEECCCHHHHCC
IVPCGVTEHGVTSLVDLGLPVTLDEADAALKASFRRVFGEVEDAAAPF
CCCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFGRDDDRPAGWAVSTGLVDYPAAVAAMEARAAAIAEGSAGELVWLLEHPPLYTAGVSAK
CCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCC
PADLLDAGRFPVFESGRGGQFTYHGPGQRVAYVMLDLTKRGRDVRAFVGALEAWVIGALD
CHHHHCCCCCCEEECCCCCEEEEECCCCEEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHH
AFNVRGEIREGRVGVWVERRAPGVPPREDKIAAIGVKLRRWVSFHGIALNVEPDLSHFSG
HHCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHHEECCEEEEECCCHHHHCC
IVPCGVTEHGVTSLVDLGLPVTLDEADAALKASFRRVFGEVEDAAAPF
CCCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]