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The map label for this gene is nylA [H]

Identifier: 197104666

GI number: 197104666

Start: 1384245

End: 1385648

Strand: Direct

Name: nylA [H]

Synonym: PHZ_c1200

Alternate gene names: 197104666

Gene position: 1384245-1385648 (Clockwise)

Preceding gene: 197104665

Following gene: 197104671

Centisome position: 34.64

GC content: 74.29

Gene sequence:

>1404_bases
ATGCGGTTCGAAGAGTACCGGGCGCACGATGCGGTGGGGCTGGCCGAGCTTGTGGCCCGCGGCGAGGTCTCGGCCGGCGA
GCTGCTGGACGCGGCGGTCACGCGGATGGCGGAGGTCAACCCGCGGATCAACGCCGTCACCCTGGACCTGTCCGACGTGG
CGCGGACGGCGGCGCCCGGATCGGGGCCGCTCGCGGGCGTCCCGTTTCTGCTGAAGGACCTGGGGGCGACGCTGGCCGGG
ACGGTCACCAGCGGCGGCTCGAAGCTGTTCGCGGACGCCGCGGCGGAGGCCGACAGCGCTACGACCCGGCTCTACAAGGC
CGCGGGGCTCAACATCTTCGGCAAGACCAACACGCCCGAGTTCGGCCTGTGGCCGGTCACCGAATCCGAGCACCTGGGCG
TCTGCCGCAATCCCTGGGATCTCGCCCGCACGCCGGGCGGCTCGTCGGGCGGGGCGGCGGCGGCGGTCGCGGCCGGGATC
GTGCCGGCGGCCCACGCCAGCGACGGCGGCGGCTCGATCCGCACGCCGGCCGCCTGCTGCGGCCTGTTCGGGATGAAGCC
GTCGCGGGGGCGGGTCAGCTTCGCGCCGCAGGGCGAGGGCTGGGCCGGCGCCTCGGTGCAGCACGCGGTGACGCGGTCGG
TCCGGGACAGCGCCGCCCTGCTGGACGCCGTCTGCGCGCCGCAGCCGGGCGATCCCTATTTCCTGCCGCCGCCCGAGCGG
CCGTTCCTGGAGGCGGCGGGCCGCGATCCCGGCCGGCTGCGGATCGGCTTCACCACCGCGGCGCTGCAGTCGCAGGCGCT
GGACCCCGAATGCGCCGAGGCCGTGCGCGACGCCGCGAAGCTCTGCGAGGACCTCGGCCATGCCGTGGAAGAGGTGAAGG
TCCCCGGCGACTTCCCCGCCATGCAGGCGGCCGCCGGCCTGGTGATCGCCGCCAGCGTCGCGGCAAACCTCGACGCCGAG
GCCGAACGCCGCGGCCGGCCCGTCGGGAAGGGCGAGGTCGAGGGGCTGACGATGGCCACCTACCGGCGGGGGCAGGGGGT
GACCGGCTCGGCCTACGTCCAGGCGCTGGCGACGCTGCACGCCTTCGGCCGCGACGTGGCGGCGCTGTTCGAGACCTACG
ATGTGCTGCTGCTCTCGACCCTGGGGCGTCCGGCGATTCCGATCGGCTGGATCTTCGAGGACCGGGACCAGATCGCCGAT
CGCCTGTTCAGCTTCATGCCGAACACCCAACCCTTCAACAATTCGGGGCAGCCGGCGATGACCGTGCCGCTGGCCTGGAG
CGGGGAGGGCTTGCCGATCGGCCTGCAGTTCGTCGGCCGCACGGGCGAGGAGGCGATGCTGTTCAGCCTGGCGGGCCAGC
TGGAGCGGGCCCGGCCCTGGTTCGACAGGGTCGCGCCGCTCTAG

Upstream 100 bases:

>100_bases
CGCCGCCTATGGGGGGAAGCCGCCCGCTTCCGCAAGGGCGCGGTTGGACGGCGGGCGCGCCTGTGCTTGCCTGGCCGGAT
AACCAGACAGGGAGGGTCCG

Downstream 100 bases:

>100_bases
GCCCTTCGCGCGCGGCGCTGCTGGATCAGCCAGGCGCCGATCACGCCCGTGGCCACCGCCCCGATCAGCAGGGTCGAGAC
GGCGTTGATCTCGGGGTTCA

Product: 6-aminohexanoate-cyclic-dimer hydrolase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MRFEEYRAHDAVGLAELVARGEVSAGELLDAAVTRMAEVNPRINAVTLDLSDVARTAAPGSGPLAGVPFLLKDLGATLAG
TVTSGGSKLFADAAAEADSATTRLYKAAGLNIFGKTNTPEFGLWPVTESEHLGVCRNPWDLARTPGGSSGGAAAAVAAGI
VPAAHASDGGGSIRTPAACCGLFGMKPSRGRVSFAPQGEGWAGASVQHAVTRSVRDSAALLDAVCAPQPGDPYFLPPPER
PFLEAAGRDPGRLRIGFTTAALQSQALDPECAEAVRDAAKLCEDLGHAVEEVKVPGDFPAMQAAAGLVIAASVAANLDAE
AERRGRPVGKGEVEGLTMATYRRGQGVTGSAYVQALATLHAFGRDVAALFETYDVLLLSTLGRPAIPIGWIFEDRDQIAD
RLFSFMPNTQPFNNSGQPAMTVPLAWSGEGLPIGLQFVGRTGEEAMLFSLAGQLERARPWFDRVAPL

Sequences:

>Translated_467_residues
MRFEEYRAHDAVGLAELVARGEVSAGELLDAAVTRMAEVNPRINAVTLDLSDVARTAAPGSGPLAGVPFLLKDLGATLAG
TVTSGGSKLFADAAAEADSATTRLYKAAGLNIFGKTNTPEFGLWPVTESEHLGVCRNPWDLARTPGGSSGGAAAAVAAGI
VPAAHASDGGGSIRTPAACCGLFGMKPSRGRVSFAPQGEGWAGASVQHAVTRSVRDSAALLDAVCAPQPGDPYFLPPPER
PFLEAAGRDPGRLRIGFTTAALQSQALDPECAEAVRDAAKLCEDLGHAVEEVKVPGDFPAMQAAAGLVIAASVAANLDAE
AERRGRPVGKGEVEGLTMATYRRGQGVTGSAYVQALATLHAFGRDVAALFETYDVLLLSTLGRPAIPIGWIFEDRDQIAD
RLFSFMPNTQPFNNSGQPAMTVPLAWSGEGLPIGLQFVGRTGEEAMLFSLAGQLERARPWFDRVAPL
>Mature_467_residues
MRFEEYRAHDAVGLAELVARGEVSAGELLDAAVTRMAEVNPRINAVTLDLSDVARTAAPGSGPLAGVPFLLKDLGATLAG
TVTSGGSKLFADAAAEADSATTRLYKAAGLNIFGKTNTPEFGLWPVTESEHLGVCRNPWDLARTPGGSSGGAAAAVAAGI
VPAAHASDGGGSIRTPAACCGLFGMKPSRGRVSFAPQGEGWAGASVQHAVTRSVRDSAALLDAVCAPQPGDPYFLPPPER
PFLEAAGRDPGRLRIGFTTAALQSQALDPECAEAVRDAAKLCEDLGHAVEEVKVPGDFPAMQAAAGLVIAASVAANLDAE
AERRGRPVGKGEVEGLTMATYRRGQGVTGSAYVQALATLHAFGRDVAALFETYDVLLLSTLGRPAIPIGWIFEDRDQIAD
RLFSFMPNTQPFNNSGQPAMTVPLAWSGEGLPIGLQFVGRTGEEAMLFSLAGQLERARPWFDRVAPL

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=27.4, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI195972892, Length=235, Percent_Identity=32.3404255319149, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI166795287, Length=283, Percent_Identity=30.7420494699647, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=309, Percent_Identity=30.7443365695793, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17537465, Length=491, Percent_Identity=24.847250509165, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17556276, Length=323, Percent_Identity=30.3405572755418, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=323, Percent_Identity=30.3405572755418, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=306, Percent_Identity=29.4117647058824, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=298, Percent_Identity=26.8456375838926, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=423, Percent_Identity=25.2955082742317, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24644968, Length=490, Percent_Identity=25.5102040816327, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648435, Length=486, Percent_Identity=25.7201646090535, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648437, Length=486, Percent_Identity=25.7201646090535, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI45550774, Length=486, Percent_Identity=25.7201646090535, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648113, Length=435, Percent_Identity=30.3448275862069, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI161078093, Length=487, Percent_Identity=25.2566735112936, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356731, Length=237, Percent_Identity=31.2236286919831, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24652985, Length=488, Percent_Identity=25.4098360655738, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922090, Length=488, Percent_Identity=25.4098360655738, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24652981, Length=488, Percent_Identity=25.4098360655738, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24652983, Length=488, Percent_Identity=25.4098360655738, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648441, Length=417, Percent_Identity=25.8992805755396, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648439, Length=417, Percent_Identity=25.8992805755396, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 48584; Mature: 48584

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFEEYRAHDAVGLAELVARGEVSAGELLDAAVTRMAEVNPRINAVTLDLSDVARTAAPG
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCC
SGPLAGVPFLLKDLGATLAGTVTSGGSKLFADAAAEADSATTRLYKAAGLNIFGKTNTPE
CCCCCCHHHHHHHHCHHHEEEECCCCCHHHHHHHHCCCHHHHHHHHHHCCEEEECCCCCC
FGLWPVTESEHLGVCRNPWDLARTPGGSSGGAAAAVAAGIVPAAHASDGGGSIRTPAACC
CCEECCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
GLFGMKPSRGRVSFAPQGEGWAGASVQHAVTRSVRDSAALLDAVCAPQPGDPYFLPPPER
HHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PFLEAAGRDPGRLRIGFTTAALQSQALDPECAEAVRDAAKLCEDLGHAVEEVKVPGDFPA
HHHHHCCCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
MQAAAGLVIAASVAANLDAEAERRGRPVGKGEVEGLTMATYRRGQGVTGSAYVQALATLH
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
AFGRDVAALFETYDVLLLSTLGRPAIPIGWIFEDRDQIADRLFSFMPNTQPFNNSGQPAM
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEE
TVPLAWSGEGLPIGLQFVGRTGEEAMLFSLAGQLERARPWFDRVAPL
EEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MRFEEYRAHDAVGLAELVARGEVSAGELLDAAVTRMAEVNPRINAVTLDLSDVARTAAPG
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCC
SGPLAGVPFLLKDLGATLAGTVTSGGSKLFADAAAEADSATTRLYKAAGLNIFGKTNTPE
CCCCCCHHHHHHHHCHHHEEEECCCCCHHHHHHHHCCCHHHHHHHHHHCCEEEECCCCCC
FGLWPVTESEHLGVCRNPWDLARTPGGSSGGAAAAVAAGIVPAAHASDGGGSIRTPAACC
CCEECCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
GLFGMKPSRGRVSFAPQGEGWAGASVQHAVTRSVRDSAALLDAVCAPQPGDPYFLPPPER
HHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PFLEAAGRDPGRLRIGFTTAALQSQALDPECAEAVRDAAKLCEDLGHAVEEVKVPGDFPA
HHHHHCCCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
MQAAAGLVIAASVAANLDAEAERRGRPVGKGEVEGLTMATYRRGQGVTGSAYVQALATLH
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
AFGRDVAALFETYDVLLLSTLGRPAIPIGWIFEDRDQIADRLFSFMPNTQPFNNSGQPAM
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCEE
TVPLAWSGEGLPIGLQFVGRTGEEAMLFSLAGQLERARPWFDRVAPL
EEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]