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The map label for this gene is mepA [H]

Identifier: 197104441

GI number: 197104441

Start: 1077140

End: 1078237

Strand: Reverse

Name: mepA [H]

Synonym: PHZ_c0975

Alternate gene names: 197104441

Gene position: 1078237-1077140 (Counterclockwise)

Preceding gene: 197104444

Following gene: 197104440

Centisome position: 26.98

GC content: 74.59

Gene sequence:

>1098_bases
TTGGCGCTTGTTGGCTGCGGACGGAAGGAGGGACCGCCCGTCGCGGCCGCCCCCGCGGTTTCGGTCGCCACGCCCCTGAA
GGCGACGGTGCGCGACTGGGACGACTTCACGGGACGCTTCGAGGCCTCGCACCGGGTGGACGTGCGCGCCCGCGCCGGCG
GTTACCTCCAGGCCGTGCACTTCCAGGAGGGCCAGACGGTCCGCAAGGGCCAGCTCCTCTTCACCCTCGATCCGCGTCCC
GCCCAGGCCCAGCTCGCCGCCGCCCGCGCCCAGGCCGACCTGGCCCGCGCCGAGCTGAAGCGGGCCGAGAGCCTGCTGGC
CGCGACCGCGATCTCGCAGGAGGAGTACGAGAACCGCCGCTCGGCCGCCGCGGTCGCCGAGGCGACCCTGCGCGCCCGCC
AGCTCGACGTGGAGTTCACCCGCGTCACCGCGCCCATCTCGGGCCGCATCTCGGCCCGGCGGGTGGATCCCGGCAACCTG
ATCGCCGGCGGCACCTCCGCGGGCGACGTGCTGACCACCATCGTCGCCGCCGACCCCATCCACTTCATCTTCGACGCCTC
GGAGGCCCAGCTGCTGAAGCTGCAGCGCCAGACCGCCGGCAAGGGCGGGGTGCCGGTGCAGGTGCGGCTGCAGGACGAAG
CGGACTACCGCTGGACCGGGACGGTGGATTTCCTCGACAACGCCGTGGACCCGGCCTCGGGCACGATCCGGCTGCGCGCC
CGCATCCGCAATCCGGACGGCTTCCTGAAGGAAGGCATGTACGGCTCGGCCCGGATGGCCGGCGCCGGCGCCTACGAGGC
GCTGCTGATCCCCGAGACCGCCGTGGCCTCGGACGGTCCGCGCAAGGTAGCCTATGTCGTGGCCGCGGACGGCCAGGCGC
AGAGCCGGGCGCTGCAGCTCGGCCCCGTCGTGGACGGCCTGCGGGTGATCCGCGCCGGCCTGAACCCGACCGACCGGGTC
GTGGTGAACGGCGTCCAGCGCGTCCGCCCAGGCCAGAAGGTCCAGCCCAAGACCGTCACCATCAGCCGCACGGCCCAGGC
GGCCGAGCGGCCCGCCCCGGCCGCGGCGCCGGCCTCCACCGCCACCATCGTCCGCTAG

Upstream 100 bases:

>100_bases
ATCACACGTTGACTTGATGCCGCGCGCGCCTCCCCGCGCGCCGAGGGGATAGAAATCGATGCGGACCTTCCGAACGCTGG
CGCTCACCGCGCTGGCGAGC

Downstream 100 bases:

>100_bases
GCCGCCCCGCCAGATGAGCTTCTCCCGCTTCTTCATCGACCGGCCGATCTTCGCCGGCGTCATCTCGGTGTTCGTGGCCC
TGCTCGGCCTCTTCGCCTAC

Product: multidrug-efflux system secretion protein, HlyD family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MALVGCGRKEGPPVAAAPAVSVATPLKATVRDWDDFTGRFEASHRVDVRARAGGYLQAVHFQEGQTVRKGQLLFTLDPRP
AQAQLAAARAQADLARAELKRAESLLAATAISQEEYENRRSAAAVAEATLRARQLDVEFTRVTAPISGRISARRVDPGNL
IAGGTSAGDVLTTIVAADPIHFIFDASEAQLLKLQRQTAGKGGVPVQVRLQDEADYRWTGTVDFLDNAVDPASGTIRLRA
RIRNPDGFLKEGMYGSARMAGAGAYEALLIPETAVASDGPRKVAYVVAADGQAQSRALQLGPVVDGLRVIRAGLNPTDRV
VVNGVQRVRPGQKVQPKTVTISRTAQAAERPAPAAAPASTATIVR

Sequences:

>Translated_365_residues
MALVGCGRKEGPPVAAAPAVSVATPLKATVRDWDDFTGRFEASHRVDVRARAGGYLQAVHFQEGQTVRKGQLLFTLDPRP
AQAQLAAARAQADLARAELKRAESLLAATAISQEEYENRRSAAAVAEATLRARQLDVEFTRVTAPISGRISARRVDPGNL
IAGGTSAGDVLTTIVAADPIHFIFDASEAQLLKLQRQTAGKGGVPVQVRLQDEADYRWTGTVDFLDNAVDPASGTIRLRA
RIRNPDGFLKEGMYGSARMAGAGAYEALLIPETAVASDGPRKVAYVVAADGQAQSRALQLGPVVDGLRVIRAGLNPTDRV
VVNGVQRVRPGQKVQPKTVTISRTAQAAERPAPAAAPASTATIVR
>Mature_364_residues
ALVGCGRKEGPPVAAAPAVSVATPLKATVRDWDDFTGRFEASHRVDVRARAGGYLQAVHFQEGQTVRKGQLLFTLDPRPA
QAQLAAARAQADLARAELKRAESLLAATAISQEEYENRRSAAAVAEATLRARQLDVEFTRVTAPISGRISARRVDPGNLI
AGGTSAGDVLTTIVAADPIHFIFDASEAQLLKLQRQTAGKGGVPVQVRLQDEADYRWTGTVDFLDNAVDPASGTIRLRAR
IRNPDGFLKEGMYGSARMAGAGAYEALLIPETAVASDGPRKVAYVVAADGQAQSRALQLGPVVDGLRVIRAGLNPTDRVV
VNGVQRVRPGQKVQPKTVTISRTAQAAERPAPAAAPASTATIVR

Specific function: The periplasmic linker protein component of an organic solvent and antibiotic efflux pump; confers resistance to toluene, hexane, p-xylene, ampicillin, penicillin G, erythromycin, novobiocin and tetracycline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=349, Percent_Identity=33.5243553008596, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1789929, Length=353, Percent_Identity=30.028328611898, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1786668, Length=382, Percent_Identity=30.6282722513089, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1789665, Length=374, Percent_Identity=29.9465240641711, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87081796, Length=319, Percent_Identity=25.0783699059561, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38633; Mature: 38502

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVGCGRKEGPPVAAAPAVSVATPLKATVRDWDDFTGRFEASHRVDVRARAGGYLQAVH
CCEEECCCCCCCCCCCCCCCHHCCCCHHHHHCCHHCCCEECCCCEEEEEECCCCEEEEEE
FQEGQTVRKGQLLFTLDPRPAQAQLAAARAQADLARAELKRAESLLAATAISQEEYENRR
ECCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAAAVAEATLRARQLDVEFTRVTAPISGRISARRVDPGNLIAGGTSAGDVLTTIVAADPI
HHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHCCCE
HFIFDASEAQLLKLQRQTAGKGGVPVQVRLQDEADYRWTGTVDFLDNAVDPASGTIRLRA
EEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEHHHHHCCCCCCCCEEEEEE
RIRNPDGFLKEGMYGSARMAGAGAYEALLIPETAVASDGPRKVAYVVAADGQAQSRALQL
EECCCCCHHHCCCCCCCEECCCCCEEEEEECCHHHCCCCCCEEEEEEEECCCCHHCEEEE
GPVVDGLRVIRAGLNPTDRVVVNGVQRVRPGQKVQPKTVTISRTAQAAERPAPAAAPAST
CCHHHHHHHHHCCCCCHHHHHHCCHHHCCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCC
ATIVR
EEECC
>Mature Secondary Structure 
ALVGCGRKEGPPVAAAPAVSVATPLKATVRDWDDFTGRFEASHRVDVRARAGGYLQAVH
CEEECCCCCCCCCCCCCCCHHCCCCHHHHHCCHHCCCEECCCCEEEEEECCCCEEEEEE
FQEGQTVRKGQLLFTLDPRPAQAQLAAARAQADLARAELKRAESLLAATAISQEEYENRR
ECCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAAAVAEATLRARQLDVEFTRVTAPISGRISARRVDPGNLIAGGTSAGDVLTTIVAADPI
HHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHCCCE
HFIFDASEAQLLKLQRQTAGKGGVPVQVRLQDEADYRWTGTVDFLDNAVDPASGTIRLRA
EEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEHHHHHCCCCCCCCEEEEEE
RIRNPDGFLKEGMYGSARMAGAGAYEALLIPETAVASDGPRKVAYVVAADGQAQSRALQL
EECCCCCHHHCCCCCCCEECCCCCEEEEEECCHHHCCCCCCEEEEEEEECCCCHHCEEEE
GPVVDGLRVIRAGLNPTDRVVVNGVQRVRPGQKVQPKTVTISRTAQAAERPAPAAAPAST
CCHHHHHHHHHCCCCCHHHHHHCCHHHCCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCC
ATIVR
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9827328 [H]