| Definition | Phenylobacterium zucineum HLK1, complete genome. |
|---|---|
| Accession | NC_011144 |
| Length | 3,996,255 |
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The map label for this gene is ispG [H]
Identifier: 197104302
GI number: 197104302
Start: 920612
End: 921754
Strand: Reverse
Name: ispG [H]
Synonym: PHZ_c0836
Alternate gene names: 197104302
Gene position: 921754-920612 (Counterclockwise)
Preceding gene: 197104303
Following gene: 197104301
Centisome position: 23.07
GC content: 69.73
Gene sequence:
>1143_bases ATGAGCGCGCAAGATCCCAACCACCACCGGCCCTGGCGGGCGATCCATCGGCGGCCCTCGCGCAAGATCCGCGTCGGCGC GGTCGAGGTCGGGGGCGATGCGCCGATCACCGTCCAGTCGATGACCAACACGGTCACCGCCGACGCGGCGGCGACGATCG ACCAGATCCGCCGGCTGGAGGAGGCCGGGGCCGACATCGTGCGGGTCTCGTGCCCGGACGAGGAGTCCACCGCCGCGTTC CGGACCATCGCGCGCGAGGCCATGGTTCCGCTGGTGGCCGACATCCACTTCCACTACCGGCGCGGAATCGAGGCGGCGCA GGCGGGCGCGGCCTGCCTGCGGATCAATCCGGGCAACATCGGCAGCCCCGACCGGGTGCGCGAGGTGATCCAGGCGGCGC GCGACCACGGCTGCTCCATGCGTATCGGGGTCAACGCCGGTTCGCTGGAGCGCGAGCTGCTGGAGCGCTACGGCGAGCCC TGCCCCGAGGCCATGGTCGAGAGCGCGCTGAACCACGCGCGCATCCTGCAGGACCACGACTTCCACGAGTTCAAGATCAG CGTGAAGGCGTCGGACCCGTTCATGACGGTCGCCGCCTACCAGCAGCTGGCCGAGGCCATCGACTGCCCGCTGCACCTGG GGGTGACCGAGGCCGGCGCCCTGCGCACCGGGACGGTGAAGAGCGCCATCGGCATCGGCTCGCTGCTGTGGGCCGGGATC GGCGACACCATCCGCGTCAGCCTGGCGGCCGACCCGGTGGAGGAGATCAAGGTCGGCTTCGACATCCTGAAGTCGCTGGG CCTGCGCCACCGGGGCGTGAACATCATCGCCTGCCCCTCATGCGCGCGGCAGGGCTTCAACGTCATCGAGACGGTCGAGA AGCTGGAGCAGCGGCTGGCGCACATCTCCACCTCGATGAGCCTGTCGATCATCGGCTGCGTCGTGAACGGCCCGGGCGAG GCGCTGATGACCGACCTGGGCTTCACCGGCGGCGGCAAGGGCTCGGGCATGGTCTATGTCGCCGGACGTCCCGATCACAA GATGGACAACGACCGGATGGTCGACCACATCGTCGAACTGGTCGAAGCCAAGGCGGCCGAGATCGAGGCGGCGAAGAAGG CCCCGCTGCAGGCCGCCGAATAA
Upstream 100 bases:
>100_bases GCTGGCGCCCCCGCGCCGCTGACCGGCAGACGACGGCGCGCGGGGCCGATCGCCGCGGCGGGTTGGGAAAAGCCGCCAAC TCGCCTATTTTCCGCCCGGC
Downstream 100 bases:
>100_bases CGCAGAAAGTTGACGCCAGCGTCACTCTTGCGAAGCGGTCTCATCCGCGCTTAGGTGGCGGCCCATTTCTTCGGAGGGAC ACCATGTCCGCGGACGGCAG
Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
Products: NA
Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]
Number of amino acids: Translated: 380; Mature: 379
Protein sequence:
>380_residues MSAQDPNHHRPWRAIHRRPSRKIRVGAVEVGGDAPITVQSMTNTVTADAAATIDQIRRLEEAGADIVRVSCPDEESTAAF RTIAREAMVPLVADIHFHYRRGIEAAQAGAACLRINPGNIGSPDRVREVIQAARDHGCSMRIGVNAGSLERELLERYGEP CPEAMVESALNHARILQDHDFHEFKISVKASDPFMTVAAYQQLAEAIDCPLHLGVTEAGALRTGTVKSAIGIGSLLWAGI GDTIRVSLAADPVEEIKVGFDILKSLGLRHRGVNIIACPSCARQGFNVIETVEKLEQRLAHISTSMSLSIIGCVVNGPGE ALMTDLGFTGGGKGSGMVYVAGRPDHKMDNDRMVDHIVELVEAKAAEIEAAKKAPLQAAE
Sequences:
>Translated_380_residues MSAQDPNHHRPWRAIHRRPSRKIRVGAVEVGGDAPITVQSMTNTVTADAAATIDQIRRLEEAGADIVRVSCPDEESTAAF RTIAREAMVPLVADIHFHYRRGIEAAQAGAACLRINPGNIGSPDRVREVIQAARDHGCSMRIGVNAGSLERELLERYGEP CPEAMVESALNHARILQDHDFHEFKISVKASDPFMTVAAYQQLAEAIDCPLHLGVTEAGALRTGTVKSAIGIGSLLWAGI GDTIRVSLAADPVEEIKVGFDILKSLGLRHRGVNIIACPSCARQGFNVIETVEKLEQRLAHISTSMSLSIIGCVVNGPGE ALMTDLGFTGGGKGSGMVYVAGRPDHKMDNDRMVDHIVELVEAKAAEIEAAKKAPLQAAE >Mature_379_residues SAQDPNHHRPWRAIHRRPSRKIRVGAVEVGGDAPITVQSMTNTVTADAAATIDQIRRLEEAGADIVRVSCPDEESTAAFR TIAREAMVPLVADIHFHYRRGIEAAQAGAACLRINPGNIGSPDRVREVIQAARDHGCSMRIGVNAGSLERELLERYGEPC PEAMVESALNHARILQDHDFHEFKISVKASDPFMTVAAYQQLAEAIDCPLHLGVTEAGALRTGTVKSAIGIGSLLWAGIG DTIRVSLAADPVEEIKVGFDILKSLGLRHRGVNIIACPSCARQGFNVIETVEKLEQRLAHISTSMSLSIIGCVVNGPGEA LMTDLGFTGGGKGSGMVYVAGRPDHKMDNDRMVDHIVELVEAKAAEIEAAKKAPLQAAE
Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]
COG id: COG0821
COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the ispG family [H]
Homologues:
Organism=Escherichia coli, GI1788863, Length=366, Percent_Identity=57.9234972677596, Blast_Score=428, Evalue=1e-121,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013785 - InterPro: IPR011005 - InterPro: IPR016425 - InterPro: IPR004588 [H]
Pfam domain/function: PF04551 GcpE [H]
EC number: =1.17.7.1 [H]
Molecular weight: Translated: 40791; Mature: 40660
Theoretical pI: Translated: 6.39; Mature: 6.39
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.1 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 5.0 %Cys+Met (Translated Protein) 2.1 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 4.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSAQDPNHHRPWRAIHRRPSRKIRVGAVEVGGDAPITVQSMTNTVTADAAATIDQIRRLE CCCCCCCCCCCHHHHHCCCCCEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHH EAGADIVRVSCPDEESTAAFRTIAREAMVPLVADIHFHYRRGIEAAQAGAACLRINPGNI HCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCC GSPDRVREVIQAARDHGCSMRIGVNAGSLERELLERYGEPCPEAMVESALNHARILQDHD CCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC FHEFKISVKASDPFMTVAAYQQLAEAIDCPLHLGVTEAGALRTGTVKSAIGIGSLLWAGI CEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCCEECCCHHHHHHHHHHHHHCC GDTIRVSLAADPVEEIKVGFDILKSLGLRHRGVNIIACPSCARQGFNVIETVEKLEQRLA CCEEEEEEECCCHHHHHHHHHHHHHCCCHHCCCEEEECCHHHHHCCHHHHHHHHHHHHHH HISTSMSLSIIGCVVNGPGEALMTDLGFTGGGKGSGMVYVAGRPDHKMDNDRMVDHIVEL HHHCCCEEEEEEEEECCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH VEAKAAEIEAAKKAPLQAAE HHHHHHHHHHHHCCCCCCCC >Mature Secondary Structure SAQDPNHHRPWRAIHRRPSRKIRVGAVEVGGDAPITVQSMTNTVTADAAATIDQIRRLE CCCCCCCCCCHHHHHCCCCCEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHH EAGADIVRVSCPDEESTAAFRTIAREAMVPLVADIHFHYRRGIEAAQAGAACLRINPGNI HCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCC GSPDRVREVIQAARDHGCSMRIGVNAGSLERELLERYGEPCPEAMVESALNHARILQDHD CCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC FHEFKISVKASDPFMTVAAYQQLAEAIDCPLHLGVTEAGALRTGTVKSAIGIGSLLWAGI CEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCCEECCCHHHHHHHHHHHHHCC GDTIRVSLAADPVEEIKVGFDILKSLGLRHRGVNIIACPSCARQGFNVIETVEKLEQRLA CCEEEEEEECCCHHHHHHHHHHHHHCCCHHCCCEEEECCHHHHHCCHHHHHHHHHHHHHH HISTSMSLSIIGCVVNGPGEALMTDLGFTGGGKGSGMVYVAGRPDHKMDNDRMVDHIVEL HHHCCCEEEEEEEEECCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH VEAKAAEIEAAKKAPLQAAE HHHHHHHHHHHHCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11259647 [H]