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The map label for this gene is prfA [H]

Identifier: 197104299

GI number: 197104299

Start: 918665

End: 919798

Strand: Reverse

Name: prfA [H]

Synonym: PHZ_c0833

Alternate gene names: 197104299

Gene position: 919798-918665 (Counterclockwise)

Preceding gene: 197104301

Following gene: 197104297

Centisome position: 23.02

GC content: 70.63

Gene sequence:

>1134_bases
TTGGCTTCGGCGGCGAGCTCCGCTAGAGGAAGCAACCTTCGAAAACGGACATCCAAGTTGCGACTTCCCCAGGCCAGGCT
CGACCAGGTTCTCGACCGCTTCCGCGAGATCGAAGCCCGCATGGGCGCGGCCTCGGACGGGGCCGAGATCGTGCGGCTGT
CGAAGGAGCACGCCGAGCTGAAGCCGGTGGCCGACGCGGTCCAGGCGCTGGAGCGCGCCCGCGCCGAGCAGCCCGAGCTG
GAGGAGATGGCCGCTTCGGGCGACGCCGAGATGGCCGCCCTCGCCCGCGACGAGCTGGAGGCGCTGAAAGAGCGGCTGCC
CGAGCTGGAGCGCGAGGTCGCCCTGCTGCTGGCGCCCAAGGACAAGGACGAGAACGCCTCGGCCATCCTGGAGGTGCGCG
CCGGCACCGGCGGGGACGAGGCGGCGCTGTTCGCCGGCGACCTGTTCCGCATGTACCAGCGCTACGCCCAGACCCGCGGC
TGGCGCGTGGAGATCGACAGCATCTCGGAGGGCGACGCCGGGGGCTACAAGGAGATCATCGCCTCGATCACCGGCGAGGG
CGTGTTCGGCCGGCTGAAGTTCGAGAGCGGCGTCCACCGGGTCCAGCGGGTGCCCGCGACCGAGGCGCAGGGGCGCATCC
ACACCTCGGCCGCCACCGTGGCCGTCCTGCCCGAGGTGGAGGACGTGGAGATCGAGGTCCGCGACCAGGACATCCGCATC
GACACCTACCGCTCGTCGGGCGCCGGCGGCCAGCACGTGAACAAGACGGACTCGGCCGTGCGCATCACCCACCTGCCCAC
CGGCATCGTGGTCACCAGCTCGGAGAAGTCGCAGCACCAGAACCGCGCCCGGGCGATGAAGGTGCTGAAGGCCCGCCTTT
ACGAGCAGCAGCGCGAGGCGCTGGACTCGGCCCGCGCCGAAAGCCGCAAGAGCCAGGTGGGCTCGGGCGACCGCTCGGAG
CGGATTCGCACCTACAACTTCCCGCAGGGGCGGGTGACCGACCACCGCATCAACCTGACCCTCTACAACCTGGCGAAGGT
GATGGAGGGCGAGGCGCTGGACGACGTGATCGAGCCGCTGATCGCCGAGGACCAGGCCGCGCGCCTGGCTGCCCTCGAGG
ACGAGTTCGCCTAG

Upstream 100 bases:

>100_bases
GTCAGGCCGTCGTTGCGCTCGGTGACTCGTTCAGTCGCCATTTCATAGTCTCCTCAGGTCACCGGCCCCAAACGGGCGTG
AGCGTGGGGCGTTCCGCCGT

Downstream 100 bases:

>100_bases
GCGCCGAACCGTTCGCGGTAGGCGCTGGGGGCCACGCCCAGCTGGCGTCTGAAGGCGCGGCGCAAGTTCTGCTCGTCGGC
GAAGCCGCAGGCGCGGGCCA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MASAASSARGSNLRKRTSKLRLPQARLDQVLDRFREIEARMGAASDGAEIVRLSKEHAELKPVADAVQALERARAEQPEL
EEMAASGDAEMAALARDELEALKERLPELEREVALLLAPKDKDENASAILEVRAGTGGDEAALFAGDLFRMYQRYAQTRG
WRVEIDSISEGDAGGYKEIIASITGEGVFGRLKFESGVHRVQRVPATEAQGRIHTSAATVAVLPEVEDVEIEVRDQDIRI
DTYRSSGAGGQHVNKTDSAVRITHLPTGIVVTSSEKSQHQNRARAMKVLKARLYEQQREALDSARAESRKSQVGSGDRSE
RIRTYNFPQGRVTDHRINLTLYNLAKVMEGEALDDVIEPLIAEDQAARLAALEDEFA

Sequences:

>Translated_377_residues
MASAASSARGSNLRKRTSKLRLPQARLDQVLDRFREIEARMGAASDGAEIVRLSKEHAELKPVADAVQALERARAEQPEL
EEMAASGDAEMAALARDELEALKERLPELEREVALLLAPKDKDENASAILEVRAGTGGDEAALFAGDLFRMYQRYAQTRG
WRVEIDSISEGDAGGYKEIIASITGEGVFGRLKFESGVHRVQRVPATEAQGRIHTSAATVAVLPEVEDVEIEVRDQDIRI
DTYRSSGAGGQHVNKTDSAVRITHLPTGIVVTSSEKSQHQNRARAMKVLKARLYEQQREALDSARAESRKSQVGSGDRSE
RIRTYNFPQGRVTDHRINLTLYNLAKVMEGEALDDVIEPLIAEDQAARLAALEDEFA
>Mature_376_residues
ASAASSARGSNLRKRTSKLRLPQARLDQVLDRFREIEARMGAASDGAEIVRLSKEHAELKPVADAVQALERARAEQPELE
EMAASGDAEMAALARDELEALKERLPELEREVALLLAPKDKDENASAILEVRAGTGGDEAALFAGDLFRMYQRYAQTRGW
RVEIDSISEGDAGGYKEIIASITGEGVFGRLKFESGVHRVQRVPATEAQGRIHTSAATVAVLPEVEDVEIEVRDQDIRID
TYRSSGAGGQHVNKTDSAVRITHLPTGIVVTSSEKSQHQNRARAMKVLKARLYEQQREALDSARAESRKSQVGSGDRSER
IRTYNFPQGRVTDHRINLTLYNLAKVMEGEALDDVIEPLIAEDQAARLAALEDEFA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=297, Percent_Identity=45.4545454545455, Blast_Score=268, Evalue=6e-72,
Organism=Homo sapiens, GI34577120, Length=334, Percent_Identity=41.0179640718563, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI166795305, Length=202, Percent_Identity=44.0594059405941, Blast_Score=183, Evalue=3e-46,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=58.4269662921348, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI2367172, Length=311, Percent_Identity=40.1929260450161, Blast_Score=208, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=274, Percent_Identity=40.8759124087591, Blast_Score=198, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=332, Percent_Identity=43.9759036144578, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI19921226, Length=370, Percent_Identity=41.3513513513514, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41481; Mature: 41350

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAASSARGSNLRKRTSKLRLPQARLDQVLDRFREIEARMGAASDGAEIVRLSKEHAEL
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KPVADAVQALERARAEQPELEEMAASGDAEMAALARDELEALKERLPELEREVALLLAPK
HHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
DKDENASAILEVRAGTGGDEAALFAGDLFRMYQRYAQTRGWRVEIDSISEGDAGGYKEII
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHH
ASITGEGVFGRLKFESGVHRVQRVPATEAQGRIHTSAATVAVLPEVEDVEIEVRDQDIRI
HHHCCCCEEEEEEHHHHHHHHHHCCCCCCCCEEECCCEEEEECCCCCCCEEEEECCEEEE
DTYRSSGAGGQHVNKTDSAVRITHLPTGIVVTSSEKSQHQNRARAMKVLKARLYEQQREA
EEEECCCCCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSARAESRKSQVGSGDRSERIRTYNFPQGRVTDHRINLTLYNLAKVMEGEALDDVIEPL
HHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHH
IAEDQAARLAALEDEFA
HCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ASAASSARGSNLRKRTSKLRLPQARLDQVLDRFREIEARMGAASDGAEIVRLSKEHAEL
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KPVADAVQALERARAEQPELEEMAASGDAEMAALARDELEALKERLPELEREVALLLAPK
HHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
DKDENASAILEVRAGTGGDEAALFAGDLFRMYQRYAQTRGWRVEIDSISEGDAGGYKEII
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHH
ASITGEGVFGRLKFESGVHRVQRVPATEAQGRIHTSAATVAVLPEVEDVEIEVRDQDIRI
HHHCCCCEEEEEEHHHHHHHHHHCCCCCCCCEEECCCEEEEECCCCCCCEEEEECCEEEE
DTYRSSGAGGQHVNKTDSAVRITHLPTGIVVTSSEKSQHQNRARAMKVLKARLYEQQREA
EEEECCCCCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSARAESRKSQVGSGDRSERIRTYNFPQGRVTDHRINLTLYNLAKVMEGEALDDVIEPL
HHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHH
IAEDQAARLAALEDEFA
HCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]