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The map label for this gene is folA [H]

Identifier: 197104283

GI number: 197104283

Start: 902351

End: 902869

Strand: Reverse

Name: folA [H]

Synonym: PHZ_c0817

Alternate gene names: 197104283

Gene position: 902869-902351 (Counterclockwise)

Preceding gene: 197104284

Following gene: 197104281

Centisome position: 22.59

GC content: 70.91

Gene sequence:

>519_bases
ATGTCGCACGTGATCCTTTCCGCCGGGCCCATCGCGCGGGCCCGCAACGGCGTCATCGGCAAGGACGGCGCCCTGCCCTG
GCGGCTGAAGTCCGACCTGGCGCTGTTCAAGCGCACCACCCTGGGCAAGCCGGTGATCATGGGCCGCAAGACCTGGGAGA
GCCTCCCCAAGCGCCCCCTGCCCGGCCGCACCAACATCGTGCTGACCCGCGACCAGAGCTACGAGGCGCCGGGGGCGCTG
GTGGTCGAGGACTTCTCGGAGGCCGTGCAGATCGCCCGCGAGCAGGCGGCCGAGGACGGCGCGGACGAGGTGTGCGTGAT
CGGCGGCGCCTCGCTGTTCGAGCTGGCGCTGAAGAAGGCCCAGCGGCTCTACCTCACCGAGGTGCATGCCGACGTCGAGG
GAGACGTGACGCTCTCGCCCATCGACGAGAGCCGCTGGCGCGAAGTCTCCCGCGAGAACCACCCCGCGTCCGACGCCGAC
GACTTCCCCTTCACCTGGCGGGTGCTGGAGCGCCTCTGA

Upstream 100 bases:

>100_bases
TCATCCTGATCGTCGCCGCGATCGTCATCGCGCAGCGCGCCGGCCTCTTCTAGCCGGGATCGGCTTGGTCGGGACGCCCG
GCGCGGGCTAGAACCCGGCC

Downstream 100 bases:

>100_bases
AAAAGGACACCGGGACACGCCCGAGGGCGGTCCCGGCGTTCAGGGGAGGAACCGGGACCTAGAAGCTGAGGGTCAGCGAG
GCGCCGTAGGTGCGCGGCTG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MSHVILSAGPIARARNGVIGKDGALPWRLKSDLALFKRTTLGKPVIMGRKTWESLPKRPLPGRTNIVLTRDQSYEAPGAL
VVEDFSEAVQIAREQAAEDGADEVCVIGGASLFELALKKAQRLYLTEVHADVEGDVTLSPIDESRWREVSRENHPASDAD
DFPFTWRVLERL

Sequences:

>Translated_172_residues
MSHVILSAGPIARARNGVIGKDGALPWRLKSDLALFKRTTLGKPVIMGRKTWESLPKRPLPGRTNIVLTRDQSYEAPGAL
VVEDFSEAVQIAREQAAEDGADEVCVIGGASLFELALKKAQRLYLTEVHADVEGDVTLSPIDESRWREVSRENHPASDAD
DFPFTWRVLERL
>Mature_171_residues
SHVILSAGPIARARNGVIGKDGALPWRLKSDLALFKRTTLGKPVIMGRKTWESLPKRPLPGRTNIVLTRDQSYEAPGALV
VEDFSEAVQIAREQAAEDGADEVCVIGGASLFELALKKAQRLYLTEVHADVEGDVTLSPIDESRWREVSRENHPASDADD
FPFTWRVLERL

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=150, Percent_Identity=32.6666666666667, Blast_Score=78, Evalue=3e-15,
Organism=Homo sapiens, GI28827793, Length=150, Percent_Identity=32, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1786233, Length=161, Percent_Identity=44.0993788819876, Blast_Score=119, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17505899, Length=180, Percent_Identity=34.4444444444444, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324810, Length=163, Percent_Identity=28.8343558282209, Blast_Score=63, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116008026, Length=181, Percent_Identity=34.2541436464088, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24647458, Length=181, Percent_Identity=34.2541436464088, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19046; Mature: 18915

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVILSAGPIARARNGVIGKDGALPWRLKSDLALFKRTTLGKPVIMGRKTWESLPKRPL
CCCEEECCCCCHHHCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCC
PGRTNIVLTRDQSYEAPGALVVEDFSEAVQIAREQAAEDGADEVCVIGGASLFELALKKA
CCCCCEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHH
QRLYLTEVHADVEGDVTLSPIDESRWREVSRENHPASDADDFPFTWRVLERL
HHHHHHHHCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHC
>Mature Secondary Structure 
SHVILSAGPIARARNGVIGKDGALPWRLKSDLALFKRTTLGKPVIMGRKTWESLPKRPL
CCEEECCCCCHHHCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCC
PGRTNIVLTRDQSYEAPGALVVEDFSEAVQIAREQAAEDGADEVCVIGGASLFELALKKA
CCCCCEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHH
QRLYLTEVHADVEGDVTLSPIDESRWREVSRENHPASDADDFPFTWRVLERL
HHHHHHHHCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]