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The map label for this gene is hpt [H]

Identifier: 197104098

GI number: 197104098

Start: 681561

End: 682088

Strand: Direct

Name: hpt [H]

Synonym: PHZ_c0632

Alternate gene names: 197104098

Gene position: 681561-682088 (Clockwise)

Preceding gene: 197104097

Following gene: 197104099

Centisome position: 17.05

GC content: 73.11

Gene sequence:

>528_bases
ATGGCTGACGCCGAGCCCGTCGTCCTGCTGTCCGAGGTGGAGATCGCCGAGCGGGTGAACGCCCTGGCGCATGCGATCGC
GCCGCGCATCGACGACGAGACGGTGGCGGTCTGCCTGCTGACCGGAGGCCTCTGGTTCTGCTCGGACCTGACCCGGGCGC
TGGGAAGGCTCGGCCGGTTCGTGCGCTTCGACGCCCTGTGGCTGGCCTCGTACCGCGACGAGCGGATCTCCAGCGGCCGC
TGCGAGGTGCGGGCCGACCTTCAGCGGCCGATCGCCGGCCGCAAGGCGCTGGTGGTGGACGACGTGTTCGACACCGGCCT
GTCGCTGTCGGAGGCGGCCCGGCTGGTGAGGGACTCCGGCGCGACCGAGGTGCTGACCGCGGTGTTCGCGCGCAAGCCCT
GGCCCACGCCCCGCGCCATCGAGCCCGACTTCGTCGCCTGGGAGGCGCCGGCCCGGTTCCTGGTCGGCTACGGCATGGAC
GCCGCCGGCCAGATGCGGGGCCTGCCGTACATCGGGGCGATGGACTAG

Upstream 100 bases:

>100_bases
TTCGAGGCGGAAGGCGCCGCGGCCGCCCCGTCCGCCGCCCAGCCGGTCGAGGCCGTCCTCGGACCCGAGCCCATAGAGGC
GCAGCCGCTGCCGGCCGGCC

Downstream 100 bases:

>100_bases
GGACCATGCCCGCGCTCACGATCCGCGAGGACGACCTCAGCCACGAGCAGACCCGAGCGCTGCTGGCGCTGCACCTGGCG
GGGATGCACGAGACCTCGCC

Product: hypoxanthine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MADAEPVVLLSEVEIAERVNALAHAIAPRIDDETVAVCLLTGGLWFCSDLTRALGRLGRFVRFDALWLASYRDERISSGR
CEVRADLQRPIAGRKALVVDDVFDTGLSLSEAARLVRDSGATEVLTAVFARKPWPTPRAIEPDFVAWEAPARFLVGYGMD
AAGQMRGLPYIGAMD

Sequences:

>Translated_175_residues
MADAEPVVLLSEVEIAERVNALAHAIAPRIDDETVAVCLLTGGLWFCSDLTRALGRLGRFVRFDALWLASYRDERISSGR
CEVRADLQRPIAGRKALVVDDVFDTGLSLSEAARLVRDSGATEVLTAVFARKPWPTPRAIEPDFVAWEAPARFLVGYGMD
AAGQMRGLPYIGAMD
>Mature_174_residues
ADAEPVVLLSEVEIAERVNALAHAIAPRIDDETVAVCLLTGGLWFCSDLTRALGRLGRFVRFDALWLASYRDERISSGRC
EVRADLQRPIAGRKALVVDDVFDTGLSLSEAARLVRDSGATEVLTAVFARKPWPTPRAIEPDFVAWEAPARFLVGYGMDA
AGQMRGLPYIGAMD

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=164, Percent_Identity=28.6585365853659, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI9910262, Length=175, Percent_Identity=27.4285714285714, Blast_Score=70, Evalue=8e-13,
Organism=Escherichia coli, GI87081689, Length=170, Percent_Identity=29.4117647058824, Blast_Score=76, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17510817, Length=174, Percent_Identity=30.4597701149425, Blast_Score=76, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 19120; Mature: 18989

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAEPVVLLSEVEIAERVNALAHAIAPRIDDETVAVCLLTGGLWFCSDLTRALGRLGRF
CCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHH
VRFDALWLASYRDERISSGRCEVRADLQRPIAGRKALVVDDVFDTGLSLSEAARLVRDSG
HHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCEEEEEHHHHCCCCHHHHHHHHHCCC
ATEVLTAVFARKPWPTPRAIEPDFVAWEAPARFLVGYGMDAAGQMRGLPYIGAMD
HHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
ADAEPVVLLSEVEIAERVNALAHAIAPRIDDETVAVCLLTGGLWFCSDLTRALGRLGRF
CCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHH
VRFDALWLASYRDERISSGRCEVRADLQRPIAGRKALVVDDVFDTGLSLSEAARLVRDSG
HHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCEEEEEHHHHCCCCHHHHHHHHHCCC
ATEVLTAVFARKPWPTPRAIEPDFVAWEAPARFLVGYGMDAAGQMRGLPYIGAMD
HHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]