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The map label for this gene is rnhB [H]

Identifier: 197104042

GI number: 197104042

Start: 611111

End: 611803

Strand: Direct

Name: rnhB [H]

Synonym: PHZ_c0576

Alternate gene names: 197104042

Gene position: 611111-611803 (Clockwise)

Preceding gene: 197104041

Following gene: 197104043

Centisome position: 15.29

GC content: 69.99

Gene sequence:

>693_bases
ATGGCCGGCAGGAGCGCGCCAAGAGCATCCAAGGGACCGACCCTGCTGCTGGAGAAGGCCTTCGTCGGCGGGCCGGTCTG
CGGGGTGGACGAGGCGGGGCGCGGCCCCTGGGCCGGACCGGTCTGCGCCGGCGCGGTGATCCTCGATTCCAATCCGCGCA
AGGTCCCCAAGGGCCTCGACGATTCCAAGAAGCTCTCGGCCAAGGCGCGAGAGGCGCTGGAGGAGGAGATCAAGGAGAAG
GCCGTGGCCTGGGGCGTCGGCTTCGCCAGCGTCTCCGAGATCGCCAGCCTGAACATCCTGCACGCCACGGGCCTGGCCAT
GTGCCGGGCTATCGAGGCCATGGCGGTGCAGCCCGCCTTCGCCCTGGTGGACGGCAACTACCGCTTCGACCTGCCGTGCG
AGGTGACGACGGTGGTGGGGGGCGACGCCAAGTCGAAATCGATCGCCGCGGCCTCGATCCTCGCCAAGGTCGCCCGCGAC
CGGCTGATGGTGGAGATGGACGCGCTCTATCCCGGCTACGGCTTCGCCAGCCACAAGGGCTACAACGCCCCCAGCCACGT
CGAGGCCCTGGTCAGCCTCGGCCCCTGCGAGATCCATCGCCTGGCCTGGCGGCCGGTGAAGCTTGTCCTGGCCGGCAAGG
ATCCGAGGCTCGCGGGCGACCTCCAGGACGAGCTGCCGTTCGGCGAGGCTTAA

Upstream 100 bases:

>100_bases
GTCCGGTGATCGTCTACGCCTGGATGCAGGCCACCGGCCTGGTCAACGACCACCTGACCTGCTGCTTCCGCCACGAGCAG
GTGAAGGGGCTGGCGCGCTG

Downstream 100 bases:

>100_bases
CTTCTCGTTGACCATCCGCGTCAACGGCCTCTTCACCATAAGCCCCGAGGATTCAGACTCTGGTTCTCTTGGGGTTTGAG
CATGGGCCTGCCGGTCGACA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MAGRSAPRASKGPTLLLEKAFVGGPVCGVDEAGRGPWAGPVCAGAVILDSNPRKVPKGLDDSKKLSAKAREALEEEIKEK
AVAWGVGFASVSEIASLNILHATGLAMCRAIEAMAVQPAFALVDGNYRFDLPCEVTTVVGGDAKSKSIAAASILAKVARD
RLMVEMDALYPGYGFASHKGYNAPSHVEALVSLGPCEIHRLAWRPVKLVLAGKDPRLAGDLQDELPFGEA

Sequences:

>Translated_230_residues
MAGRSAPRASKGPTLLLEKAFVGGPVCGVDEAGRGPWAGPVCAGAVILDSNPRKVPKGLDDSKKLSAKAREALEEEIKEK
AVAWGVGFASVSEIASLNILHATGLAMCRAIEAMAVQPAFALVDGNYRFDLPCEVTTVVGGDAKSKSIAAASILAKVARD
RLMVEMDALYPGYGFASHKGYNAPSHVEALVSLGPCEIHRLAWRPVKLVLAGKDPRLAGDLQDELPFGEA
>Mature_229_residues
AGRSAPRASKGPTLLLEKAFVGGPVCGVDEAGRGPWAGPVCAGAVILDSNPRKVPKGLDDSKKLSAKAREALEEEIKEKA
VAWGVGFASVSEIASLNILHATGLAMCRAIEAMAVQPAFALVDGNYRFDLPCEVTTVVGGDAKSKSIAAASILAKVARDR
LMVEMDALYPGYGFASHKGYNAPSHVEALVSLGPCEIHRLAWRPVKLVLAGKDPRLAGDLQDELPFGEA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=185, Percent_Identity=52.4324324324324, Blast_Score=175, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 24102; Mature: 23971

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRSAPRASKGPTLLLEKAFVGGPVCGVDEAGRGPWAGPVCAGAVILDSNPRKVPKGLD
CCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHCEEEECCCCCCCCCCCC
DSKKLSAKAREALEEEIKEKAVAWGVGFASVSEIASLNILHATGLAMCRAIEAMAVQPAF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ALVDGNYRFDLPCEVTTVVGGDAKSKSIAAASILAKVARDRLMVEMDALYPGYGFASHKG
EEEECCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
YNAPSHVEALVSLGPCEIHRLAWRPVKLVLAGKDPRLAGDLQDELPFGEA
CCCHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
AGRSAPRASKGPTLLLEKAFVGGPVCGVDEAGRGPWAGPVCAGAVILDSNPRKVPKGLD
CCCCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHCEEEECCCCCCCCCCCC
DSKKLSAKAREALEEEIKEKAVAWGVGFASVSEIASLNILHATGLAMCRAIEAMAVQPAF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ALVDGNYRFDLPCEVTTVVGGDAKSKSIAAASILAKVARDRLMVEMDALYPGYGFASHKG
EEEECCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
YNAPSHVEALVSLGPCEIHRLAWRPVKLVLAGKDPRLAGDLQDELPFGEA
CCCHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA