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The map label for this gene is ppsA [H]

Identifier: 197103923

GI number: 197103923

Start: 467288

End: 469729

Strand: Direct

Name: ppsA [H]

Synonym: PHZ_c0457

Alternate gene names: 197103923

Gene position: 467288-469729 (Clockwise)

Preceding gene: 197103922

Following gene: 197103924

Centisome position: 11.69

GC content: 73.14

Gene sequence:

>2442_bases
ATGGCGCCTGCCCACTACATCCGACCCTTCGACACCCTGGCCCTTGGGGACGTGCCCCTGGTGGGCGGCAAGACCGCCTC
GCTGGGCGAGCTGCAGCAGCTGCTGAAGGGGGCGAAGGTCGCCGTGCCCGAGGGGTTCGCGATCACCGCCCAGGCGTACC
GGGACGCGCTGACCGGGGCCGGCGCCTGGGAGCCGCTGCGGGCGCTGCTGGAAGGACTGGACGTCGAGGACGTGGCCGAG
CTGGCGCGCCGGGCGGCCGAGGCCCGGCGGATCGTCTACGAGGCGACAGGCGGCGAGGACCTGCGCCGCCAGATCGTCGA
GGGCTTCCGTGAGCTGAAGCGGGCGGTGGGGGCCGACATCAGCGTGGCGGTGCGCAGCTCGGCCACCGCCGAGGACCTGC
CGACAGCCAGCTTCGCCGGCCAGCACGAGAGCTTCCTGCACGTGCGCGACGAGGCCGCGCTGATCGAGGCGTGCCGCCGC
TGCTTCGCGTCGCTGTTCACCGACCGGGCGGTGGTCTACCGGACCCACAACGGCTTCGACCACCTGAAGGTCGCGCTCTC
CATCGCGGTGATGCGGATGGTCCGTTCCGACCGGGCGGCCAGCGGGGTGACCTTCACCCTGGACACCGAGAGCGGGTTCC
GGGACGTGGTGTTCGTCACCGGCGCCTACGGCCTGGGCGAGAACGTGGTGCAGGGCCTCGTCGACCCGGACGAGTTCTAC
GTCCACAAGCCGACGTTCCGGCAGGGCTACCGCGCGGTGCTGAAGCGCAGCCTGGGGGCCAAGCAGACCCGCATCGTCTG
GGACCGCAGGGCCGGCGGCGCGGCGCTGAAGACCTTGCCGGTCGGCAGGGCCGAGCGCGGCCGCTACTGCCTGACCGACG
CCGAGGTCATGGACCTGACCGACGTGGCGATCCGCATCGAGGACCACTACTCCAAGCGCGCCGGGCGGCCGACGCCGATG
GACATCGAGTGGGCCAAGGACGCGGTGGACGGGCGGCTCTACGTCGTCCAGGCGCGGCCCGAGACCGTGGCCTCGCAGCG
CGGCCAGACCCTGGAGACCTTCCGCCTGACCGGCTCGGGACCCGTGCTCGCGACCGGCAAGGCGGTGGGCGAGCGGATCG
CCTCGGGACGGGTGCGGGTGATCCGCGGCCCCGCGGACCTGCGGGCGTTCCAGGAAGGCGAGGTGCTGGTGGCGCCCGCG
ACCAGCCCAGACTGGGAGCCGGTGATGAAGCGCGCGGCGGCCGTGGTCACCGACCGCGGCGGGCGCACCTGCCACGCCGC
CATCGTCGCCCGGGAGCTGGGCCTGCCGGCGGTGGTGGGGGCGGGGGACGCCACGGCGCGGCTGGGGGACGGCGGCCTCG
TCACCGTCTCCTGCGCCGAGGGCGAGACCGGCCGGGTCTACCAGGGGCGGGTCCCGTTCGAGGTGACGCGGCTGGACCTG
GCCTCGCTGGCGACGCCCGTCACGGAGGTGATGCTGAACCTGGCGCATCCCGAGCTGGCCTTCCGGTTCGCCCAGCTGCC
CTCGGCGGGCGTGGGCCTGGCCAGGCTGGAGTTCGTGATCGGCGAGCAGATCCGGCTGCACCCGATGGCGGCGGCGCATC
CGGACCGGGTGACCTCGGCCCGGCAACGGGCGCAGATCGCCCGGCTGTCGCGCGGCTTTCCCTCGCCGCGGGCCTATTTC
GTCGAGCGGCTGGTGGAGGGCGCGGGCGTGATCGCGGCCGCCTTCCACCCGCGGCCGGTGATCGTCCGGCTGTCGGACTT
CAAGACCAACGAGTACGCCCGCCTGACCGGCGGGGCCGACTTCGAGCCCCGCGAGGAGAACCCGATGCTGGGGTTCCGGG
GCGCCTCCCGCTACGCCCATCCGCTCTACGCCGACGGCTTCGCCCTGGAGTGCGAGGCCCTGGCGCGGGTGCGGCGGGAC
CTCGGCCTGAAGAACCTGAAGGTGATGATCCCCTTCTGCCGGCGGGTGGAGGAGGCCCGGCGCGTGCTGGACGCCATGGC
CGCCAACGGCCTGAAGCGCGGCGAGGACGGGCTGGAGGTCTATGTGATGTGCGAGATCCCCAACAACGTCATCCAGATCG
ACGCCTTCGCCGAGCTGTTCGACGGGTTCTCGATCGGCTCAAACGACCTGACCCAGCTCGTGCTGGGCGTGGACCGCGAC
TCCGAGACGGTGGCCTTCGACTTCGACGAGCGCGACCCGGGCGTGCTGGAGATGCTGCGCCAGGCGGTGACCGGGGCGCA
CCGCAACGGCCGGCACGTGGGCATCTGCGGCGAGGCGCCGGCGACCTATCCCGAGGTCGCGGCGTTTCTCGCCGGCGTCG
GCATCGACTCCATCAGCGTCAATCCGCAGAGCCTGCCGCGCACGCTGGCCGCGGTGCGCGAGGCGGAAGCCGCGGCCGCC
AAGGCGCCGGCCCGGGAGCTGTCGAAGATCGGCGGTGTTTGA

Upstream 100 bases:

>100_bases
TTCAGCGGAACGCGGCCGACCTGATCCCGATTGACCTCACTCAAGGCGCGCGCCGGCCGCGCGCCTAGGTTGCTCCCCTC
GCACCTTGCGGAGCGGACCC

Downstream 100 bases:

>100_bases
GCCGGATCAAACCGGCCCCGGCGCCGGCCGTGGTAGACGTCGGATCGTTCCGGTGTGGCTTTGCGGCTCGGCCGGAACGG
GGCCAGCGGCCAAACGGCCA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 813; Mature: 812

Protein sequence:

>813_residues
MAPAHYIRPFDTLALGDVPLVGGKTASLGELQQLLKGAKVAVPEGFAITAQAYRDALTGAGAWEPLRALLEGLDVEDVAE
LARRAAEARRIVYEATGGEDLRRQIVEGFRELKRAVGADISVAVRSSATAEDLPTASFAGQHESFLHVRDEAALIEACRR
CFASLFTDRAVVYRTHNGFDHLKVALSIAVMRMVRSDRAASGVTFTLDTESGFRDVVFVTGAYGLGENVVQGLVDPDEFY
VHKPTFRQGYRAVLKRSLGAKQTRIVWDRRAGGAALKTLPVGRAERGRYCLTDAEVMDLTDVAIRIEDHYSKRAGRPTPM
DIEWAKDAVDGRLYVVQARPETVASQRGQTLETFRLTGSGPVLATGKAVGERIASGRVRVIRGPADLRAFQEGEVLVAPA
TSPDWEPVMKRAAAVVTDRGGRTCHAAIVARELGLPAVVGAGDATARLGDGGLVTVSCAEGETGRVYQGRVPFEVTRLDL
ASLATPVTEVMLNLAHPELAFRFAQLPSAGVGLARLEFVIGEQIRLHPMAAAHPDRVTSARQRAQIARLSRGFPSPRAYF
VERLVEGAGVIAAAFHPRPVIVRLSDFKTNEYARLTGGADFEPREENPMLGFRGASRYAHPLYADGFALECEALARVRRD
LGLKNLKVMIPFCRRVEEARRVLDAMAANGLKRGEDGLEVYVMCEIPNNVIQIDAFAELFDGFSIGSNDLTQLVLGVDRD
SETVAFDFDERDPGVLEMLRQAVTGAHRNGRHVGICGEAPATYPEVAAFLAGVGIDSISVNPQSLPRTLAAVREAEAAAA
KAPARELSKIGGV

Sequences:

>Translated_813_residues
MAPAHYIRPFDTLALGDVPLVGGKTASLGELQQLLKGAKVAVPEGFAITAQAYRDALTGAGAWEPLRALLEGLDVEDVAE
LARRAAEARRIVYEATGGEDLRRQIVEGFRELKRAVGADISVAVRSSATAEDLPTASFAGQHESFLHVRDEAALIEACRR
CFASLFTDRAVVYRTHNGFDHLKVALSIAVMRMVRSDRAASGVTFTLDTESGFRDVVFVTGAYGLGENVVQGLVDPDEFY
VHKPTFRQGYRAVLKRSLGAKQTRIVWDRRAGGAALKTLPVGRAERGRYCLTDAEVMDLTDVAIRIEDHYSKRAGRPTPM
DIEWAKDAVDGRLYVVQARPETVASQRGQTLETFRLTGSGPVLATGKAVGERIASGRVRVIRGPADLRAFQEGEVLVAPA
TSPDWEPVMKRAAAVVTDRGGRTCHAAIVARELGLPAVVGAGDATARLGDGGLVTVSCAEGETGRVYQGRVPFEVTRLDL
ASLATPVTEVMLNLAHPELAFRFAQLPSAGVGLARLEFVIGEQIRLHPMAAAHPDRVTSARQRAQIARLSRGFPSPRAYF
VERLVEGAGVIAAAFHPRPVIVRLSDFKTNEYARLTGGADFEPREENPMLGFRGASRYAHPLYADGFALECEALARVRRD
LGLKNLKVMIPFCRRVEEARRVLDAMAANGLKRGEDGLEVYVMCEIPNNVIQIDAFAELFDGFSIGSNDLTQLVLGVDRD
SETVAFDFDERDPGVLEMLRQAVTGAHRNGRHVGICGEAPATYPEVAAFLAGVGIDSISVNPQSLPRTLAAVREAEAAAA
KAPARELSKIGGV
>Mature_812_residues
APAHYIRPFDTLALGDVPLVGGKTASLGELQQLLKGAKVAVPEGFAITAQAYRDALTGAGAWEPLRALLEGLDVEDVAEL
ARRAAEARRIVYEATGGEDLRRQIVEGFRELKRAVGADISVAVRSSATAEDLPTASFAGQHESFLHVRDEAALIEACRRC
FASLFTDRAVVYRTHNGFDHLKVALSIAVMRMVRSDRAASGVTFTLDTESGFRDVVFVTGAYGLGENVVQGLVDPDEFYV
HKPTFRQGYRAVLKRSLGAKQTRIVWDRRAGGAALKTLPVGRAERGRYCLTDAEVMDLTDVAIRIEDHYSKRAGRPTPMD
IEWAKDAVDGRLYVVQARPETVASQRGQTLETFRLTGSGPVLATGKAVGERIASGRVRVIRGPADLRAFQEGEVLVAPAT
SPDWEPVMKRAAAVVTDRGGRTCHAAIVARELGLPAVVGAGDATARLGDGGLVTVSCAEGETGRVYQGRVPFEVTRLDLA
SLATPVTEVMLNLAHPELAFRFAQLPSAGVGLARLEFVIGEQIRLHPMAAAHPDRVTSARQRAQIARLSRGFPSPRAYFV
ERLVEGAGVIAAAFHPRPVIVRLSDFKTNEYARLTGGADFEPREENPMLGFRGASRYAHPLYADGFALECEALARVRRDL
GLKNLKVMIPFCRRVEEARRVLDAMAANGLKRGEDGLEVYVMCEIPNNVIQIDAFAELFDGFSIGSNDLTQLVLGVDRDS
ETVAFDFDERDPGVLEMLRQAVTGAHRNGRHVGICGEAPATYPEVAAFLAGVGIDSISVNPQSLPRTLAAVREAEAAAAK
APARELSKIGGV

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=786, Percent_Identity=50.2544529262086, Blast_Score=781, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=449, Percent_Identity=26.9487750556793, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI48994992, Length=423, Percent_Identity=29.0780141843972, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788726, Length=239, Percent_Identity=30.9623430962343, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1789193, Length=475, Percent_Identity=27.1578947368421, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17564524, Length=236, Percent_Identity=28.3898305084746, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87788; Mature: 87657

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPAHYIRPFDTLALGDVPLVGGKTASLGELQQLLKGAKVAVPEGFAITAQAYRDALTGA
CCCCCCCCCCCHHEECCCCCCCCCCCCHHHHHHHHCCCEEECCCCCEEEHHHHHHHHCCC
GAWEPLRALLEGLDVEDVAELARRAAEARRIVYEATGGEDLRRQIVEGFRELKRAVGADI
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCE
SVAVRSSATAEDLPTASFAGQHESFLHVRDEAALIEACRRCFASLFTDRAVVYRTHNGFD
EEEEECCCCHHHCCCCHHCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCHH
HLKVALSIAVMRMVRSDRAASGVTFTLDTESGFRDVVFVTGAYGLGENVVQGLVDPDEFY
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHCCCCCCEE
VHKPTFRQGYRAVLKRSLGAKQTRIVWDRRAGGAALKTLPVGRAERGRYCLTDAEVMDLT
EECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCCCCCCCEEECCCHHHHHE
DVAIRIEDHYSKRAGRPTPMDIEWAKDAVDGRLYVVQARPETVASQRGQTLETFRLTGSG
EEEEEEEHHHHHHCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHCCCCEEEEEEEECCC
PVLATGKAVGERIASGRVRVIRGPADLRAFQEGEVLVAPATSPDWEPVMKRAAAVVTDRG
CEEECCHHHHHHHHCCCEEEEECCHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCC
GRTCHAAIVARELGLPAVVGAGDATARLGDGGLVTVSCAEGETGRVYQGRVPFEVTRLDL
CCEEHHHHHHHHCCCCEEEECCCCCEEECCCCEEEEEECCCCCCCEEECCCCEEEHHHHH
ASLATPVTEVMLNLAHPELAFRFAQLPSAGVGLARLEFVIGEQIRLHPMAAAHPDRVTSA
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHH
RQRAQIARLSRGFPSPRAYFVERLVEGAGVIAAAFHPRPVIVRLSDFKTNEYARLTGGAD
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCEEEECCCCC
FEPREENPMLGFRGASRYAHPLYADGFALECEALARVRRDLGLKNLKVMIPFCRRVEEAR
CCCCCCCCCEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RVLDAMAANGLKRGEDGLEVYVMCEIPNNVIQIDAFAELFDGFSIGSNDLTQLVLGVDRD
HHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEHHHHHHHCCCCCCCHHHHHHHEECCCC
SETVAFDFDERDPGVLEMLRQAVTGAHRNGRHVGICGEAPATYPEVAAFLAGVGIDSISV
CCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
NPQSLPRTLAAVREAEAAAAKAPARELSKIGGV
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure 
APAHYIRPFDTLALGDVPLVGGKTASLGELQQLLKGAKVAVPEGFAITAQAYRDALTGA
CCCCCCCCCCHHEECCCCCCCCCCCCHHHHHHHHCCCEEECCCCCEEEHHHHHHHHCCC
GAWEPLRALLEGLDVEDVAELARRAAEARRIVYEATGGEDLRRQIVEGFRELKRAVGADI
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCE
SVAVRSSATAEDLPTASFAGQHESFLHVRDEAALIEACRRCFASLFTDRAVVYRTHNGFD
EEEEECCCCHHHCCCCHHCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCHH
HLKVALSIAVMRMVRSDRAASGVTFTLDTESGFRDVVFVTGAYGLGENVVQGLVDPDEFY
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHCCCCCCEE
VHKPTFRQGYRAVLKRSLGAKQTRIVWDRRAGGAALKTLPVGRAERGRYCLTDAEVMDLT
EECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCCCCCCCEEECCCHHHHHE
DVAIRIEDHYSKRAGRPTPMDIEWAKDAVDGRLYVVQARPETVASQRGQTLETFRLTGSG
EEEEEEEHHHHHHCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHCCCCEEEEEEEECCC
PVLATGKAVGERIASGRVRVIRGPADLRAFQEGEVLVAPATSPDWEPVMKRAAAVVTDRG
CEEECCHHHHHHHHCCCEEEEECCHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCC
GRTCHAAIVARELGLPAVVGAGDATARLGDGGLVTVSCAEGETGRVYQGRVPFEVTRLDL
CCEEHHHHHHHHCCCCEEEECCCCCEEECCCCEEEEEECCCCCCCEEECCCCEEEHHHHH
ASLATPVTEVMLNLAHPELAFRFAQLPSAGVGLARLEFVIGEQIRLHPMAAAHPDRVTSA
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHH
RQRAQIARLSRGFPSPRAYFVERLVEGAGVIAAAFHPRPVIVRLSDFKTNEYARLTGGAD
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCEEEECCCCC
FEPREENPMLGFRGASRYAHPLYADGFALECEALARVRRDLGLKNLKVMIPFCRRVEEAR
CCCCCCCCCEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RVLDAMAANGLKRGEDGLEVYVMCEIPNNVIQIDAFAELFDGFSIGSNDLTQLVLGVDRD
HHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEHHHHHHHCCCCCCCHHHHHHHEECCCC
SETVAFDFDERDPGVLEMLRQAVTGAHRNGRHVGICGEAPATYPEVAAFLAGVGIDSISV
CCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
NPQSLPRTLAAVREAEAAAAKAPARELSKIGGV
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]