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The map label for this gene is pfkB [H]

Identifier: 197103917

GI number: 197103917

Start: 459309

End: 460271

Strand: Reverse

Name: pfkB [H]

Synonym: PHZ_c0451

Alternate gene names: 197103917

Gene position: 460271-459309 (Counterclockwise)

Preceding gene: 197103921

Following gene: 197103915

Centisome position: 11.52

GC content: 75.49

Gene sequence:

>963_bases
ATGCCCGTCGTCGTCACCCTGACGCTCAACCCCGCCCTGGATGTCTCGACCTCGACGCCGCGGGTCGAGCCCGACCGCAA
GCTCCGCTGCGGCGCCGGCCGGCGCGATCCCGGCGGCGGGGGGATCAACGTCGCCCGCGTCGCCCACCGCCTGGGCGCGC
GCACGCTGGCGATCTTCCCCTCGGGCGGCCTCACCGGCCAGATGCTGGAGCGCCTCGTCGAGGCCGAAGGGGTGGGAAGC
CTGCCCGTGCCGGTCTCGGGCGAGACCCGCGAGGACGTCACCGTGCTCGACGAGTCCTCGGGCGCCCAGTACCGCTTCAT
CATGCCGGGGCCCCATCTGCACGGGGTCGAATGGATGGCCTGCCTGAAGGCGCTGGCGGGGCTCGAGGCCAAGCCCGACT
TCGTCTGCGCCAGCGGCAGCCTGCCGCCCGGCGCCCCCGACGACCTCTACGCGCGGGTGGCCGAGATCGTGGAGAGCTGG
GGCGTCCGCTTCGCCCTGGACACCTCGGGCCCGCCGCTGCAGGCGGCGCTTTCCGAACGCGTCTTCCTTATCAAGCCCAA
CCTGCGCGAGCTGCGCGAACTCACCGGCGCGCCGCTGGAGGACGAGGCGAGCCGCATCGCCGCCTGTCGGTCCCTGATCG
CGCGGGGCCGCACGGCCGCCGTGGCCCTCACCCTCGGCCACGAGGGCGCGCTGCTCGTCACCGCCCAGGACGCCTGGCGC
GCCCCCGCCCTGCCGGTGAGGCCGGTCAGCAGCGTCGGCGCCGGCGACAGCTTCCTGGGCGCCATGGTCTGGGGCCTCGC
CTCCAAGCTGACCCTGCGCGAGGCATTCGCCTTCGCCATGGCGGGCGGGTCGGCCGCCCTGCTGGCCCAGGGCACCCAGC
TCGCCGGCGCCCGCGACGTGCGCCGCCTGCTCCCGCAAGTGACCGTCGAGCGCGTCGACGACCGGGCCGCGCTGCGGGCC
TAG

Upstream 100 bases:

>100_bases
GCCGCCTTGACGCCCGTCAACCGCCGGTTTCAACCCTTGACGTGGCTCAAGGCGGGCAGGCGGGGCCCGGGCCAGCCTCC
AGGCTCGAGGAGGCCCGCCG

Downstream 100 bases:

>100_bases
CGGCGCCCCGGCGCTTGACGACTCGCCGTCAGGTGGAGTCTTCGTGCCCACCCGCCAGCGCGAGGACGGCACGTTTGCGT
ATCATCGGACTTCCGGATCA

Product: putative 6-phosphofructokinase

Products: ADP; D-fructose 1,6-bisphosphate

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MPVVVTLTLNPALDVSTSTPRVEPDRKLRCGAGRRDPGGGGINVARVAHRLGARTLAIFPSGGLTGQMLERLVEAEGVGS
LPVPVSGETREDVTVLDESSGAQYRFIMPGPHLHGVEWMACLKALAGLEAKPDFVCASGSLPPGAPDDLYARVAEIVESW
GVRFALDTSGPPLQAALSERVFLIKPNLRELRELTGAPLEDEASRIAACRSLIARGRTAAVALTLGHEGALLVTAQDAWR
APALPVRPVSSVGAGDSFLGAMVWGLASKLTLREAFAFAMAGGSAALLAQGTQLAGARDVRRLLPQVTVERVDDRAALRA

Sequences:

>Translated_320_residues
MPVVVTLTLNPALDVSTSTPRVEPDRKLRCGAGRRDPGGGGINVARVAHRLGARTLAIFPSGGLTGQMLERLVEAEGVGS
LPVPVSGETREDVTVLDESSGAQYRFIMPGPHLHGVEWMACLKALAGLEAKPDFVCASGSLPPGAPDDLYARVAEIVESW
GVRFALDTSGPPLQAALSERVFLIKPNLRELRELTGAPLEDEASRIAACRSLIARGRTAAVALTLGHEGALLVTAQDAWR
APALPVRPVSSVGAGDSFLGAMVWGLASKLTLREAFAFAMAGGSAALLAQGTQLAGARDVRRLLPQVTVERVDDRAALRA
>Mature_319_residues
PVVVTLTLNPALDVSTSTPRVEPDRKLRCGAGRRDPGGGGINVARVAHRLGARTLAIFPSGGLTGQMLERLVEAEGVGSL
PVPVSGETREDVTVLDESSGAQYRFIMPGPHLHGVEWMACLKALAGLEAKPDFVCASGSLPPGAPDDLYARVAEIVESWG
VRFALDTSGPPLQAALSERVFLIKPNLRELRELTGAPLEDEASRIAACRSLIARGRTAAVALTLGHEGALLVTAQDAWRA
PALPVRPVSSVGAGDSFLGAMVWGLASKLTLREAFAFAMAGGSAALLAQGTQLAGARDVRRLLPQVTVERVDDRAALRA

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=304, Percent_Identity=39.4736842105263, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788493, Length=278, Percent_Identity=32.7338129496403, Blast_Score=104, Evalue=8e-24,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.11

Molecular weight: Translated: 33543; Mature: 33412

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVVTLTLNPALDVSTSTPRVEPDRKLRCGAGRRDPGGGGINVARVAHRLGARTLAIFP
CCEEEEEEECCCEECCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCEEEEEC
SGGLTGQMLERLVEAEGVGSLPVPVSGETREDVTVLDESSGAQYRFIMPGPHLHGVEWMA
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHH
CLKALAGLEAKPDFVCASGSLPPGAPDDLYARVAEIVESWGVRFALDTSGPPLQAALSER
HHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHCC
VFLIKPNLRELRELTGAPLEDEASRIAACRSLIARGRTAAVALTLGHEGALLVTAQDAWR
EEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCC
APALPVRPVSSVGAGDSFLGAMVWGLASKLTLREAFAFAMAGGSAALLAQGTQLAGARDV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
RRLLPQVTVERVDDRAALRA
HHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
PVVVTLTLNPALDVSTSTPRVEPDRKLRCGAGRRDPGGGGINVARVAHRLGARTLAIFP
CEEEEEEECCCEECCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCEEEEEC
SGGLTGQMLERLVEAEGVGSLPVPVSGETREDVTVLDESSGAQYRFIMPGPHLHGVEWMA
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHH
CLKALAGLEAKPDFVCASGSLPPGAPDDLYARVAEIVESWGVRFALDTSGPPLQAALSER
HHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHCC
VFLIKPNLRELRELTGAPLEDEASRIAACRSLIARGRTAAVALTLGHEGALLVTAQDAWR
EEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCC
APALPVRPVSSVGAGDSFLGAMVWGLASKLTLREAFAFAMAGGSAALLAQGTQLAGARDV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
RRLLPQVTVERVDDRAALRA
HHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-fructose 6-phosphate

Specific reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]