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The map label for this gene is thrS

Identifier: 197103889

GI number: 197103889

Start: 430522

End: 432498

Strand: Direct

Name: thrS

Synonym: PHZ_c0423

Alternate gene names: 197103889

Gene position: 430522-432498 (Clockwise)

Preceding gene: 197103888

Following gene: 197103890

Centisome position: 10.77

GC content: 68.49

Gene sequence:

>1977_bases
ATGATCGAACTGGTTTTCCCCGACGGCTCCAAGCGTGAGTTCCCGGACGGGGTTACGGGCCGCGAGATCGCCGCCGGCAT
CTCGAAGAGCCTGGAGAAGAAGGCCATCCTGGTGGAGGTCGACGGCGAACTGCTGGACCTGGAGCGCCCGCTCGAGAAGG
GCGGGGCGTTCAAGATCCTGACGCGCGAGGCGCCCGAGGCCCTCGAGACGATCCGCCACGACGTCAGCCACATCCTGGCC
GAGGCGGTGCAGGAGCTGTTCCCCGGCACGCAGGTGACGATCGGGCCCGCGATCGAGGACGGCTTCTACTACGACTTCGC
CCGCGAGGAGCCGTTCAGCCTGGACGACCTTGCGAAGATCGAGCAGCGGATGAAGGAGATCGTCGACCGCGACGAGCCCA
TCCGCCGCGAGGAGTGGGACCGTGACGAGGCGATCCGGCACTTCCGCGAGATCGGCGAGGAGTACAAGGCCCAGATCATC
GAGGCCATTCCGGCCGGCGAGAAGATCACGGTCTACCGCCAGGGCCAGTGGAAGGACCTGTGCCGCGGCCCGCACCTGCC
GTCCACGAGGCACGCCGGCAAGGCGTTCAAGCTGACGAAGCTGGCCGGCGCCTACTGGCGCGGCGACCACCGCAACGCCC
AGCTGCAGCGCATCTACGGCACGGCCTGGGCCTCGGAAGCCGACCTCGAGGCCTACATCCAGCGCCTTGAGGAGGCCGAG
AAGCGCGACCACCGCAAGCTCGGCCGCCAGATGGACCTCTTCCATATGCAGGAAGAGGGCAAGGGCATGGTGTTCTGGCA
CGAGAAGGGCCTGACGCTGTGGCGCACGGTGGAAAGCTACGTCCGCCGCCGGCTCGACGAGGCCGGCTACCAGGAGGTGC
GCACGCCGCAGGTCCTGGACCGGGTCTTCTGGGAGAAGTCCGGCCACTGGGACAAGTACCGGCCCAACATGTTCGTCTGC
GAGACGGAGGAGGGCGAGGAGCTCTCCCTGAAGCCGATGAACTGCCCCGGCCACGTGCAGATCTTCAAGTTCGGCCAGAA
GAGCTACCGCGACCTGCCGCTGCGGATGGCCGAGTTCGGCGCCTGCCACCGCTACGAGCCGTCGGGAGCCCTGCACGGGC
TGATGCGGGTGCGCGCGTTCACGCAGGACGACGCGCACATCTTCTGCCGCGAGGACCAGATCGAGGAGGAGACGGCGCGG
TTCATCGAGCTGGCCAACTCGATCCACGCCGACTTCGGCCTGGAGCGCGACCACATCGCGCTCGCCACGCGTCCGGAGGT
GCGGGCCGGCTCGGACGAGTTCTGGGACAAGGCCGAGGCGCAGATGCTGGCGGCGGCCCGCAAGGCCGGCGTCGAGCCGG
TGATCGCCGAGGGCGACGGGGCGTTCTACGCGCCGAAGCTGGACTGGGTGCTGAAGGATTCCATCGGCCGGACGTGGACG
TGCGGCACGATCCAGCTGGACTACGTCCTGCCCGAGCGCCTGGGCGCGGAATATGTCGCCGAGGATGGATCAAAGCAGCG
GCCTGTGATGTTGCACCGGGCGATCTGCGGCTCGCTCGAGCGCTTCATCGGCGTGCTGATCGAGAACTACGCCGGCGCCT
TCCCGCTCTGGCTCGCGCCGGTGCAGGTGGTGGTGGCGACGATCACGTCCGACGCCGACGACTACGCCCGCGCGGCCGCG
GCCGAGCTGAAGGCGAAGGGCCTGCGCGTGGAACTCGACCTGCGCAACGAGAAGATCAACTACAAGGTCCGCGAGCACAG
CCTCGCGAAGGTCCCGGTGATCGCCGTCGTCGGCCGGCGTGAGGCCGAAGAGGGCAAGGTCGCCCTGAGGCGCCTCGGAT
CCGACGGCCAGCAGATCCTCGCCCTGGGCGAGGCGGCGGAAGCACTTGCCATGGAGGCGTTACCGCCTGATCTTGCGCGC
AAGCGCCAGGACGGGGGTGTCCAGGCGGGGTTGGGCGGCGAGAGGGAGGCTGGATGA

Upstream 100 bases:

>100_bases
TGACCGTGCGCCGCCTGTGCCGATGCCATCCCTGGGGGCCGTCGGGCTGGGACCCGCCGCCGCCCCCGCGCCGCAAAGGC
GCGAAATGGACGTGTGAGAC

Downstream 100 bases:

>100_bases
ACGGCATCGCCGCGTCGGTCGTCGCCGCCGAATCCGTCCTGACGACCGAGGCCCTGCGGCCGCGCCGCAGCGTCTCGGTG
GTGATGGTGGTCTACATGAC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MIELVFPDGSKREFPDGVTGREIAAGISKSLEKKAILVEVDGELLDLERPLEKGGAFKILTREAPEALETIRHDVSHILA
EAVQELFPGTQVTIGPAIEDGFYYDFAREEPFSLDDLAKIEQRMKEIVDRDEPIRREEWDRDEAIRHFREIGEEYKAQII
EAIPAGEKITVYRQGQWKDLCRGPHLPSTRHAGKAFKLTKLAGAYWRGDHRNAQLQRIYGTAWASEADLEAYIQRLEEAE
KRDHRKLGRQMDLFHMQEEGKGMVFWHEKGLTLWRTVESYVRRRLDEAGYQEVRTPQVLDRVFWEKSGHWDKYRPNMFVC
ETEEGEELSLKPMNCPGHVQIFKFGQKSYRDLPLRMAEFGACHRYEPSGALHGLMRVRAFTQDDAHIFCREDQIEEETAR
FIELANSIHADFGLERDHIALATRPEVRAGSDEFWDKAEAQMLAAARKAGVEPVIAEGDGAFYAPKLDWVLKDSIGRTWT
CGTIQLDYVLPERLGAEYVAEDGSKQRPVMLHRAICGSLERFIGVLIENYAGAFPLWLAPVQVVVATITSDADDYARAAA
AELKAKGLRVELDLRNEKINYKVREHSLAKVPVIAVVGRREAEEGKVALRRLGSDGQQILALGEAAEALAMEALPPDLAR
KRQDGGVQAGLGGEREAG

Sequences:

>Translated_658_residues
MIELVFPDGSKREFPDGVTGREIAAGISKSLEKKAILVEVDGELLDLERPLEKGGAFKILTREAPEALETIRHDVSHILA
EAVQELFPGTQVTIGPAIEDGFYYDFAREEPFSLDDLAKIEQRMKEIVDRDEPIRREEWDRDEAIRHFREIGEEYKAQII
EAIPAGEKITVYRQGQWKDLCRGPHLPSTRHAGKAFKLTKLAGAYWRGDHRNAQLQRIYGTAWASEADLEAYIQRLEEAE
KRDHRKLGRQMDLFHMQEEGKGMVFWHEKGLTLWRTVESYVRRRLDEAGYQEVRTPQVLDRVFWEKSGHWDKYRPNMFVC
ETEEGEELSLKPMNCPGHVQIFKFGQKSYRDLPLRMAEFGACHRYEPSGALHGLMRVRAFTQDDAHIFCREDQIEEETAR
FIELANSIHADFGLERDHIALATRPEVRAGSDEFWDKAEAQMLAAARKAGVEPVIAEGDGAFYAPKLDWVLKDSIGRTWT
CGTIQLDYVLPERLGAEYVAEDGSKQRPVMLHRAICGSLERFIGVLIENYAGAFPLWLAPVQVVVATITSDADDYARAAA
AELKAKGLRVELDLRNEKINYKVREHSLAKVPVIAVVGRREAEEGKVALRRLGSDGQQILALGEAAEALAMEALPPDLAR
KRQDGGVQAGLGGEREAG
>Mature_658_residues
MIELVFPDGSKREFPDGVTGREIAAGISKSLEKKAILVEVDGELLDLERPLEKGGAFKILTREAPEALETIRHDVSHILA
EAVQELFPGTQVTIGPAIEDGFYYDFAREEPFSLDDLAKIEQRMKEIVDRDEPIRREEWDRDEAIRHFREIGEEYKAQII
EAIPAGEKITVYRQGQWKDLCRGPHLPSTRHAGKAFKLTKLAGAYWRGDHRNAQLQRIYGTAWASEADLEAYIQRLEEAE
KRDHRKLGRQMDLFHMQEEGKGMVFWHEKGLTLWRTVESYVRRRLDEAGYQEVRTPQVLDRVFWEKSGHWDKYRPNMFVC
ETEEGEELSLKPMNCPGHVQIFKFGQKSYRDLPLRMAEFGACHRYEPSGALHGLMRVRAFTQDDAHIFCREDQIEEETAR
FIELANSIHADFGLERDHIALATRPEVRAGSDEFWDKAEAQMLAAARKAGVEPVIAEGDGAFYAPKLDWVLKDSIGRTWT
CGTIQLDYVLPERLGAEYVAEDGSKQRPVMLHRAICGSLERFIGVLIENYAGAFPLWLAPVQVVVATITSDADDYARAAA
AELKAKGLRVELDLRNEKINYKVREHSLAKVPVIAVVGRREAEEGKVALRRLGSDGQQILALGEAAEALAMEALPPDLAR
KRQDGGVQAGLGGEREAG

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=615, Percent_Identity=39.349593495935, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI38570111, Length=598, Percent_Identity=39.1304347826087, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI20070344, Length=632, Percent_Identity=37.1835443037975, Blast_Score=396, Evalue=1e-110,
Organism=Escherichia coli, GI1788013, Length=617, Percent_Identity=50.4051863857374, Blast_Score=646, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984184, Length=620, Percent_Identity=40.3225806451613, Blast_Score=453, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI71984192, Length=620, Percent_Identity=40.3225806451613, Blast_Score=452, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6322112, Length=630, Percent_Identity=38.4126984126984, Blast_Score=438, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6322654, Length=393, Percent_Identity=34.8600508905852, Blast_Score=255, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24583839, Length=614, Percent_Identity=39.2508143322476, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24583842, Length=614, Percent_Identity=39.2508143322476, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI28574141, Length=614, Percent_Identity=39.2508143322476, Blast_Score=447, Evalue=1e-126,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_PHEZH (B4RE95)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129266.1
- ProteinModelPortal:   B4RE95
- GeneID:   6789913
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0423
- HOGENOM:   HBG352811
- OMA:   TAKDPEC
- ProtClustDB:   PRK00413
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 74425; Mature: 74425

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELVFPDGSKREFPDGVTGREIAAGISKSLEKKAILVEVDGELLDLERPLEKGGAFKIL
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHCCHHHHHCCCCEEEE
TREAPEALETIRHDVSHILAEAVQELFPGTQVTIGPAIEDGFYYDFAREEPFSLDDLAKI
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCCCCCHHHHHHH
EQRMKEIVDRDEPIRREEWDRDEAIRHFREIGEEYKAQIIEAIPAGEKITVYRQGQWKDL
HHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHH
CRGPHLPSTRHAGKAFKLTKLAGAYWRGDHRNAQLQRIYGTAWASEADLEAYIQRLEEAE
HCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
KRDHRKLGRQMDLFHMQEEGKGMVFWHEKGLTLWRTVESYVRRRLDEAGYQEVRTPQVLD
HHHHHHHCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHCCCHHHHH
RVFWEKSGHWDKYRPNMFVCETEEGEELSLKPMNCPGHVQIFKFGQKSYRDLPLRMAEFG
HHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEECCCHHHHCCCHHHHHHC
ACHRYEPSGALHGLMRVRAFTQDDAHIFCREDQIEEETARFIELANSIHADFGLERDHIA
CCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LATRPEVRAGSDEFWDKAEAQMLAAARKAGVEPVIAEGDGAFYAPKLDWVLKDSIGRTWT
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEECCCCCHHHHHCCCCEEE
CGTIQLDYVLPERLGAEYVAEDGSKQRPVMLHRAICGSLERFIGVLIENYAGAFPLWLAP
ECEEEEEEECHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VQVVVATITSDADDYARAAAAELKAKGLRVELDLRNEKINYKVREHSLAKVPVIAVVGRR
HHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCEEEEECCC
EAEEGKVALRRLGSDGQQILALGEAAEALAMEALPPDLARKRQDGGVQAGLGGEREAG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEECCCCCCCCCC
>Mature Secondary Structure
MIELVFPDGSKREFPDGVTGREIAAGISKSLEKKAILVEVDGELLDLERPLEKGGAFKIL
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHCCHHHHHCCCCEEEE
TREAPEALETIRHDVSHILAEAVQELFPGTQVTIGPAIEDGFYYDFAREEPFSLDDLAKI
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCCCCCHHHHHHH
EQRMKEIVDRDEPIRREEWDRDEAIRHFREIGEEYKAQIIEAIPAGEKITVYRQGQWKDL
HHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHH
CRGPHLPSTRHAGKAFKLTKLAGAYWRGDHRNAQLQRIYGTAWASEADLEAYIQRLEEAE
HCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
KRDHRKLGRQMDLFHMQEEGKGMVFWHEKGLTLWRTVESYVRRRLDEAGYQEVRTPQVLD
HHHHHHHCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHCCCHHHHH
RVFWEKSGHWDKYRPNMFVCETEEGEELSLKPMNCPGHVQIFKFGQKSYRDLPLRMAEFG
HHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEECCCHHHHCCCHHHHHHC
ACHRYEPSGALHGLMRVRAFTQDDAHIFCREDQIEEETARFIELANSIHADFGLERDHIA
CCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LATRPEVRAGSDEFWDKAEAQMLAAARKAGVEPVIAEGDGAFYAPKLDWVLKDSIGRTWT
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEECCCCCHHHHHCCCCEEE
CGTIQLDYVLPERLGAEYVAEDGSKQRPVMLHRAICGSLERFIGVLIENYAGAFPLWLAP
ECEEEEEEECHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VQVVVATITSDADDYARAAAAELKAKGLRVELDLRNEKINYKVREHSLAKVPVIAVVGRR
HHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCCEEEEECCC
EAEEGKVALRRLGSDGQQILALGEAAEALAMEALPPDLARKRQDGGVQAGLGGEREAG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA