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The map label for this gene is oprM [H]

Identifier: 197103866

GI number: 197103866

Start: 402810

End: 404279

Strand: Direct

Name: oprM [H]

Synonym: PHZ_c0400

Alternate gene names: 197103866

Gene position: 402810-404279 (Clockwise)

Preceding gene: 197103853

Following gene: 197103867

Centisome position: 10.08

GC content: 75.31

Gene sequence:

>1470_bases
TTGTTCAAACAACGCCGGTCGCCCAGCCTCCAGATGAGCCGCATGCGATACGCCTCTTCAGCAGCCGTCCTGGCCGCACT
GGCGCTGGCCGCCTGCGCGAGCCCGCGCGCGGCCGACGTGCGCCTGCCCGGCGCCTACGAGGCGCCTGCCGGCGCGCCCG
TCGGCGCGGTGGCGCTGGACCAGTGGTGGACCGCCTTCGGCGACGCCGAGCTCACCGGCCTCGTCGAGCAGGCCCTCGCC
CGCAACCCGGACGTCCGCACCGCCGCGGCCCGCCTGCAGGAGGTCCGCGCCCAGCGCACGGGCGCCATCCTGCGCTACCT
GCCGCAGGGTGACGCCACAGGCTCGGCGAGCCGGACGCGCACCGAGCAGCTCGACGGCACCGAGATCAACATTCCCGGCT
TCTCCACCAGCGGCACGTCCGAGAGCTACCGGGCCAACTTCGACGTGAGCTGGGAGATCGACCTGTTCGGCCGCTCCCTC
GCCGCCCTCGGCTCGGCCAACGCCGAGGTCGCCGCCGCCCGCTTCGCCTGGGAGGGCGCGCGGGCCGCCATCGCCGCCCA
GACGGCGGACGCCTACTTCCAGGCCAAGGGCCTGGCCATCCAGCTGGCCGACGCGCGCGAGACGGCGCGGATCCAGCGCG
AGCTGTACGACCTGGCCAGCCGGCGCGCGGGCCTGGGCCTGGCCGCCTCGTCCGAGGCCGACCGCATCTCGGGCGAACTG
GCCCAGGCCGACGCCCAGGCAGCGGCGCTGGAGGCCGAGCTGCAGGCCCAGAAGCGGGCTATCCTCATTCTGGCCGGCCG
GGTGGTGGAGCCCACCGCCAACGTCGCCGCCCCGCCGGTGGTGGGCGCCGCCCCGCCCGTGCCCGCCGCCCTGCCGTCCG
AGCTGCTGGAACGCCGGCCCGACGTGCGCGAGGCCCAGGCGCGGGTCCGCTCGGCCGCCGGCCAGCAGCGGCTGGCGCAG
CTGGCGTTCTTCCCGACCTTCACCTTCAGCCCGGGCGTCGGCTGGTCGCGGCAGGAGCAGCCGGGCTTCTTCTCCGAGAG
CCAGAGCTGGACCATCGGCGGATCGGTGCTGCAGCCGATCCTGTCGATCCCGCGCCTGATGTCCGAGCTGAAGGTCCAGA
ACGCCCGCACCGAGCAGGCGGTCACCCAGTACGAGAAGACGGTCCAGACCGCCTTCCAGGAGGCCGAAGGGGCGCTGGTG
CGGCTGGACGCCGACCGCCGCCGCGTCGCCCTGCTGACCGAGGGCGAGCGCCGCGCGGCGCGGGCCTTCGAGGCGGCGCG
CACCGGCTACCAGCGCGGCCTCACCGACCTCACAACGCTCCTGAACGCCGAGCAGTCCTGGCGGGCCACCCGCGCCCAGC
TGACCTCGGCCCAGGTGCAGGCGCTGCGGCGCGCGGTCCAGGCCTACAAGGCCGTGGGCGGCGGCTGGCCTGCCCAAAGC
TACGCCCAAACCGGCCCGCAGGCCGGATGA

Upstream 100 bases:

>100_bases
GCAAAGGGCGGACTGCACGGAACCCGCCAGAGGCTCCGGCGAGGCGACGCTAAGGATTGTGCAAGGGGCGGCGGTCACCG
TTGCGCCGAACGCCCAAACT

Downstream 100 bases:

>100_bases
GACTGGCTGGATGAGACTGGCCGGATGAGACAGGCCCCGATGAGAGACGGCATGCGACTGAAGCTCACCCTCGTTCCGGT
CCTCGCGGCCCTGGCGCTGG

Product: efflux system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MFKQRRSPSLQMSRMRYASSAAVLAALALAACASPRAADVRLPGAYEAPAGAPVGAVALDQWWTAFGDAELTGLVEQALA
RNPDVRTAAARLQEVRAQRTGAILRYLPQGDATGSASRTRTEQLDGTEINIPGFSTSGTSESYRANFDVSWEIDLFGRSL
AALGSANAEVAAARFAWEGARAAIAAQTADAYFQAKGLAIQLADARETARIQRELYDLASRRAGLGLAASSEADRISGEL
AQADAQAAALEAELQAQKRAILILAGRVVEPTANVAAPPVVGAAPPVPAALPSELLERRPDVREAQARVRSAAGQQRLAQ
LAFFPTFTFSPGVGWSRQEQPGFFSESQSWTIGGSVLQPILSIPRLMSELKVQNARTEQAVTQYEKTVQTAFQEAEGALV
RLDADRRRVALLTEGERRAARAFEAARTGYQRGLTDLTTLLNAEQSWRATRAQLTSAQVQALRRAVQAYKAVGGGWPAQS
YAQTGPQAG

Sequences:

>Translated_489_residues
MFKQRRSPSLQMSRMRYASSAAVLAALALAACASPRAADVRLPGAYEAPAGAPVGAVALDQWWTAFGDAELTGLVEQALA
RNPDVRTAAARLQEVRAQRTGAILRYLPQGDATGSASRTRTEQLDGTEINIPGFSTSGTSESYRANFDVSWEIDLFGRSL
AALGSANAEVAAARFAWEGARAAIAAQTADAYFQAKGLAIQLADARETARIQRELYDLASRRAGLGLAASSEADRISGEL
AQADAQAAALEAELQAQKRAILILAGRVVEPTANVAAPPVVGAAPPVPAALPSELLERRPDVREAQARVRSAAGQQRLAQ
LAFFPTFTFSPGVGWSRQEQPGFFSESQSWTIGGSVLQPILSIPRLMSELKVQNARTEQAVTQYEKTVQTAFQEAEGALV
RLDADRRRVALLTEGERRAARAFEAARTGYQRGLTDLTTLLNAEQSWRATRAQLTSAQVQALRRAVQAYKAVGGGWPAQS
YAQTGPQAG
>Mature_489_residues
MFKQRRSPSLQMSRMRYASSAAVLAALALAACASPRAADVRLPGAYEAPAGAPVGAVALDQWWTAFGDAELTGLVEQALA
RNPDVRTAAARLQEVRAQRTGAILRYLPQGDATGSASRTRTEQLDGTEINIPGFSTSGTSESYRANFDVSWEIDLFGRSL
AALGSANAEVAAARFAWEGARAAIAAQTADAYFQAKGLAIQLADARETARIQRELYDLASRRAGLGLAASSEADRISGEL
AQADAQAAALEAELQAQKRAILILAGRVVEPTANVAAPPVVGAAPPVPAALPSELLERRPDVREAQARVRSAAGQQRLAQ
LAFFPTFTFSPGVGWSRQEQPGFFSESQSWTIGGSVLQPILSIPRLMSELKVQNARTEQAVTQYEKTVQTAFQEAEGALV
RLDADRRRVALLTEGERRAARAFEAARTGYQRGLTDLTTLLNAEQSWRATRAQLTSAQVQALRRAVQAYKAVGGGWPAQS
YAQTGPQAG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=424, Percent_Identity=26.4150943396226, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI2367348, Length=435, Percent_Identity=24.5977011494253, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52177; Mature: 52177

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKQRRSPSLQMSRMRYASSAAVLAALALAACASPRAADVRLPGAYEAPAGAPVGAVALD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHH
QWWTAFGDAELTGLVEQALARNPDVRTAAARLQEVRAQRTGAILRYLPQGDATGSASRTR
HHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
TEQLDGTEINIPGFSTSGTSESYRANFDVSWEIDLFGRSLAALGSANAEVAAARFAWEGA
HHHCCCCEEECCCCCCCCCCCCCEECCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHCCH
RAAIAAQTADAYFQAKGLAIQLADARETARIQRELYDLASRRAGLGLAASSEADRISGEL
HHHHHHHHHHHHHEECCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
AQADAQAAALEAELQAQKRAILILAGRVVEPTANVAAPPVVGAAPPVPAALPSELLERRP
HHHHHHHHHHHHHHHHHHCEEEEEECCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
DVREAQARVRSAAGQQRLAQLAFFPTFTFSPGVGWSRQEQPGFFSESQSWTIGGSVLQPI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHH
LSIPRLMSELKVQNARTEQAVTQYEKTVQTAFQEAEGALVRLDADRRRVALLTEGERRAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCHHHHH
RAFEAARTGYQRGLTDLTTLLNAEQSWRATRAQLTSAQVQALRRAVQAYKAVGGGWPAQS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
YAQTGPQAG
HHHCCCCCC
>Mature Secondary Structure
MFKQRRSPSLQMSRMRYASSAAVLAALALAACASPRAADVRLPGAYEAPAGAPVGAVALD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHH
QWWTAFGDAELTGLVEQALARNPDVRTAAARLQEVRAQRTGAILRYLPQGDATGSASRTR
HHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
TEQLDGTEINIPGFSTSGTSESYRANFDVSWEIDLFGRSLAALGSANAEVAAARFAWEGA
HHHCCCCEEECCCCCCCCCCCCCEECCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHCCH
RAAIAAQTADAYFQAKGLAIQLADARETARIQRELYDLASRRAGLGLAASSEADRISGEL
HHHHHHHHHHHHHEECCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
AQADAQAAALEAELQAQKRAILILAGRVVEPTANVAAPPVVGAAPPVPAALPSELLERRP
HHHHHHHHHHHHHHHHHHCEEEEEECCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
DVREAQARVRSAAGQQRLAQLAFFPTFTFSPGVGWSRQEQPGFFSESQSWTIGGSVLQPI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHH
LSIPRLMSELKVQNARTEQAVTQYEKTVQTAFQEAEGALVRLDADRRRVALLTEGERRAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCHHHHH
RAFEAARTGYQRGLTDLTTLLNAEQSWRATRAQLTSAQVQALRRAVQAYKAVGGGWPAQS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
YAQTGPQAG
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]