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The map label for this gene is gloA [H]

Identifier: 197103848

GI number: 197103848

Start: 385761

End: 386417

Strand: Direct

Name: gloA [H]

Synonym: PHZ_c0382

Alternate gene names: 197103848

Gene position: 385761-386417 (Clockwise)

Preceding gene: 197103847

Following gene: 197103853

Centisome position: 9.65

GC content: 67.88

Gene sequence:

>657_bases
TTGCGACTATCCCGACCTCGACATGATCGTCGGGCCCGACAACGTCTACCGCCATCGGGACGGGACGCCGTACGAGACGG
ACGCGGGGCGAAAGCGTTAGGAGCCCGACCTCTTCCGCCACCCGAAGGACGACAGATGACCCCCGATCCGCGCGATCCGG
CCACGGCCGGCTTCACCCTCAACCAGACCATGCTGCGCGTCCGCGACCCCGAGGCCTCGGTGGCCTTCTACCGCGACGTG
CTGGGGATGACCCTGCTGCAGAAGCTGGACTTCCCGCCGATGAAGTTCTCGCTCTACTTCATGGCCTATCTGGCGGACGG
CGAGATGATCCCGGACGATCCGGCCGAGCGGGCGCGGTTCATCTTCAGCCGCGAGACGACGCTGGAGCTGACCCACAACT
GGGGGACCGAGAGCGATCCGGACTTCGCCGGCTATCACAGCGGCAACGACGCGCCGCGCGGCTTCGGGCACCTGGGGATC
AGCGTGCCGGACGTGGACGCGGCCTGCGCCCGCTTCGAGACCCTGGGCGTGCCGTTCCGCAAGCGGCCGAACGACGGGGC
GATGAAGGGCATCGCCTTCATCACCGACCCGGACGGCTATTGGATCGAGATCCTGTCGCCCAACTCGATGACCGGCGTGG
TGCGCGCGGGAGCGTAG

Upstream 100 bases:

>100_bases
GCTTTCCCGCCGGCGCGCCGAACGGCCATCACATCCAGAACCGCTCGGACCGCGAGGCGGTGCTGCTGGAGGTCGGCTCG
CGCCGGCCGGCCGAGGACCA

Downstream 100 bases:

>100_bases
GCAGCCCCCTCAGAACGTGTACAGGATCGAGGCGCGGGTCGTGGTGTCGAGGTCGGAGCGGGCGACCCGGGCGTCGCGCT
CGTACTGCATGTTGTAGGAG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MRLSRPRHDRRARQRLPPSGRDAVRDGRGAKALGARPLPPPEGRQMTPDPRDPATAGFTLNQTMLRVRDPEASVAFYRDV
LGMTLLQKLDFPPMKFSLYFMAYLADGEMIPDDPAERARFIFSRETTLELTHNWGTESDPDFAGYHSGNDAPRGFGHLGI
SVPDVDAACARFETLGVPFRKRPNDGAMKGIAFITDPDGYWIEILSPNSMTGVVRAGA

Sequences:

>Translated_218_residues
MRLSRPRHDRRARQRLPPSGRDAVRDGRGAKALGARPLPPPEGRQMTPDPRDPATAGFTLNQTMLRVRDPEASVAFYRDV
LGMTLLQKLDFPPMKFSLYFMAYLADGEMIPDDPAERARFIFSRETTLELTHNWGTESDPDFAGYHSGNDAPRGFGHLGI
SVPDVDAACARFETLGVPFRKRPNDGAMKGIAFITDPDGYWIEILSPNSMTGVVRAGA
>Mature_218_residues
MRLSRPRHDRRARQRLPPSGRDAVRDGRGAKALGARPLPPPEGRQMTPDPRDPATAGFTLNQTMLRVRDPEASVAFYRDV
LGMTLLQKLDFPPMKFSLYFMAYLADGEMIPDDPAERARFIFSRETTLELTHNWGTESDPDFAGYHSGNDAPRGFGHLGI
SVPDVDAACARFETLGVPFRKRPNDGAMKGIAFITDPDGYWIEILSPNSMTGVVRAGA

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=163, Percent_Identity=62.5766871165644, Blast_Score=215, Evalue=2e-56,
Organism=Escherichia coli, GI1787940, Length=149, Percent_Identity=34.8993288590604, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323639, Length=163, Percent_Identity=44.7852760736196, Blast_Score=140, Evalue=1e-34,
Organism=Drosophila melanogaster, GI20129743, Length=159, Percent_Identity=59.748427672956, Blast_Score=200, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 24160; Mature: 24160

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRPRHDRRARQRLPPSGRDAVRDGRGAKALGARPLPPPEGRQMTPDPRDPATAGFTL
CCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
NQTMLRVRDPEASVAFYRDVLGMTLLQKLDFPPMKFSLYFMAYLADGEMIPDDPAERARF
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEECCCCCCCCCCHHHHHHE
IFSRETTLELTHNWGTESDPDFAGYHSGNDAPRGFGHLGISVPDVDAACARFETLGVPFR
EECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
KRPNDGAMKGIAFITDPDGYWIEILSPNSMTGVVRAGA
CCCCCCCCCEEEEEECCCCCEEEEECCCCCEEEEEECC
>Mature Secondary Structure
MRLSRPRHDRRARQRLPPSGRDAVRDGRGAKALGARPLPPPEGRQMTPDPRDPATAGFTL
CCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
NQTMLRVRDPEASVAFYRDVLGMTLLQKLDFPPMKFSLYFMAYLADGEMIPDDPAERARF
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEECCCCCCCCCCHHHHHHE
IFSRETTLELTHNWGTESDPDFAGYHSGNDAPRGFGHLGISVPDVDAACARFETLGVPFR
EECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
KRPNDGAMKGIAFITDPDGYWIEILSPNSMTGVVRAGA
CCCCCCCCCEEEEEECCCCCEEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]